Large-scale production of oligosaccharides using engineered bacteria.

abstract：:Small-angle X-ray scattering (SAXS) has emerged as an enabling integrative technique for comprehensive analyses of macromolecular structures and interactions in solution. Over the past two decades, SAXS has become a mainstay of the structural biologist's toolbox, supplying multiplexed measurements of molecular shape a...

journal_title：Current opinion in structural biology

authors： Brosey CA,Tainer JA

更新日期：2019-10-01 00:00:00

abstract：:PUF proteins are RNA-binding proteins named for founding members PUMILIO and fem-3 binding factor (FBF). Together these proteins represent the range of known RNA recognition properties. PUMILIO is a prototypical PUF protein whose RNA sequence specificity is simple, elegant, and predictable. FBF displays differences in...

journal_title：Current opinion in structural biology

authors： Lu G,Dolgner SJ,Hall TM

更新日期：2009-02-01 00:00:00

abstract：:Continuing investigations into protein-protein interactions have revealed their key role in regulating a wide range of cellular processes. Although efforts to modulate these interactions are more challenging and much less mature than work on conventional drug discovery pathways, significant progress has been made on s...

journal_title：Current opinion in structural biology

authors： Zhao L,Chmielewski J

更新日期：2005-02-01 00:00:00

abstract：:The first high-resolution structure of a P-type ATPase, that of the Ca(2+)-ATPase of skeletal muscle sarcoplasmic reticulum, was published in 2000. This structure has provided many clues to how the Ca(2+)-ATPase might work, but no complete answers. The Ca(2+)-ATPase structure reveals no clear pathway from the cytoplas...

journal_title：Current opinion in structural biology

authors： Lee AG

更新日期：2002-08-01 00:00:00

abstract：:There are now numerous examples of proteins that are unstructured or only partially structured under physiological conditions and yet are nevertheless functional. Such proteins are especially prevalent in eukaryotes. In many cases, intrinsically disordered proteins adopt folded structures upon binding to their biologi...

journal_title：Current opinion in structural biology

authors： Dyson HJ,Wright PE

更新日期：2002-02-01 00:00:00

abstract：:Structure-specific DNA nucleases play important roles in various DNA transactions such as DNA replication, repair and recombination. These enzymes recognize loops and branched DNA structures. Recent structural studies have provided detailed insights into the functions of these enzymes. Structures of Holliday junction ...

journal_title：Current opinion in structural biology

authors： Nishino T,Ishino Y,Morikawa K

更新日期：2006-02-01 00:00:00

abstract：:RNA structure underpins many essential functions in biology. New chemical reagents and techniques for probing RNA structure in living cells have emerged in recent years. High-throughput, genome-wide techniques such as Structure-seq2 and DMS-MaPseq exploit nucleobase modification by dimethylsulfate (DMS) to obtain comp...

journal_title：Current opinion in structural biology

authors： Mitchell D 3rd,Assmann SM,Bevilacqua PC

更新日期：2019-12-01 00:00:00

abstract：:The spliceosome executes eukaryotic precursor messenger RNA (pre-mRNA) splicing to remove noncoding introns through two sequential transesterification reactions, branching and exon ligation. The fidelity of this process is based on the recognition of the conserved sequences in the intron and dynamic compositional and ...

journal_title：Current opinion in structural biology

authors： Wan R,Bai R,Shi Y

更新日期：2019-12-01 00:00:00

abstract：:Pyrrolopyrimidine containing natural products are widely distributed in Nature. The biosynthesis of the 7-deazapurine moiety that is common to all pyrrolopyrimidines entails multiple steps, one of which is a complex radical-mediated ring contraction reaction catalyzed by CDG synthase. Herein we review the biosynthetic...

journal_title：Current opinion in structural biology

authors： Bandarian V,Drennan CL

更新日期：2015-12-01 00:00:00

abstract：:An important goal of structural genomics is to complete the structural analysis of all the enzymes in metabolic pathways and to understand the structural similarities and differences. A preliminary glimpse of this type of analysis was achieved before structural genomics efforts with the glycolytic pathway and efforts ...

journal_title：Current opinion in structural biology

authors： Erlandsen H,Abola EE,Stevens RC

更新日期：2000-12-01 00:00:00

abstract：:Protein-protein interactions involving the catalytic domain of protein kinases are likely to be generally important in the regulation of signal transduction pathways, but are rather sparsely represented in crystal structures. Recently determined structures of the kinase domains of the mitogen-activated protein kinase ...

journal_title：Current opinion in structural biology

authors： Pellicena P,Kuriyan J

更新日期：2006-12-01 00:00:00

abstract：:Recently, advances have been made in methods and applications that integrate electron microscopy density maps and comparative modeling to produce atomic structures of macromolecular assemblies. Electron microscopy can benefit from comparative modeling through the fitting of comparative models into electron microscopy ...

journal_title：Current opinion in structural biology

authors： Topf M,Sali A

更新日期：2005-10-01 00:00:00

abstract：:Telomerase is an RNP that synthesizes the 3' ends of linear chromosomes and is an important regulator of telomere length. It contains a single long non-coding telomerase RNA (TER), telomerase reverse transcriptase (TERT), and other proteins that vary among organisms. Recent progress in structural biology of telomerase...

journal_title：Current opinion in structural biology

authors： Wang Y,Feigon J

更新日期：2017-12-01 00:00:00

abstract：:For various reasons, it seems sensible to redesign or design proteins from the inside out. Past approaches in this field have involved iterations of mutagenesis and characterisation to 'evolve' designs. Increasingly, combinatorial approaches are being taken to select 'fit' sequences from libraries of variant proteins....

journal_title：Current opinion in structural biology

authors： Woolfson DN

更新日期：2001-08-01 00:00:00

abstract：:Over recent years, nuclear magnetic resonance (NMR) spectroscopy has developed into a powerful mechanistic tool for the investigation of G protein-coupled receptors (GPCRs). NMR provides insights which underpin the dynamic nature of these important receptors and reveals experimental evidence for a complex conformation...

journal_title：Current opinion in structural biology

authors： Bostock MJ,Solt AS,Nietlispach D

更新日期：2019-08-01 00:00:00

abstract：:Cyanobacteriochrome photoreceptors are linear tetrapyrrole-binding photoreceptors that are distantly related to the canonical phytochrome photoreceptors. The chromophore-binding region of the cyanobacteriochromes consists of only a cGMP-phosphodiesterase/adenylate cyclase/FhlA (GAF) domain, while that of the phytochro...

journal_title：Current opinion in structural biology

authors： Fushimi K,Narikawa R

更新日期：2019-08-01 00:00:00

abstract：:Computer simulation methods are increasingly being used to study possible conformations and dynamics of structural motifs in RNA. Recent results of molecular dynamics simulations and continum solvent studies of RNA structures and RNA-ligand complexes show promising agreement with experimental data. Combined with the o...

journal_title：Current opinion in structural biology

authors： Zacharias M

更新日期：2000-06-01 00:00:00

abstract：:The last two years have seen major advances in understanding the structural basis of bacterial cell envelope glycoconjugate biosynthesis, including capsules, lipopolysaccharide, teichoic acid, cellulose, and peptidoglycan. The recent crystal and cryo-electron microscopy structures of proteins involved in the initial g...

journal_title：Current opinion in structural biology

authors： Liston SD,Willis LM

更新日期：2021-01-08 00:00:00

abstract：:Protein-RNA interactions play essential roles in gene regulation and RNA metabolism. While high-resolution structures have revealed principles of RNA recognition by individual RNA binding domains (RBDs), the presence of multiple RBDs in many eukaryotic proteins suggests additional modes of RNA recognition by combinati...

journal_title：Current opinion in structural biology

authors： Mackereth CD,Sattler M

更新日期：2012-06-01 00:00:00

abstract：:The RNA exosome participates in the degradation and processing of a wide range of RNA molecules. Recent advances in understanding how the exosome is organized and functions largely stem from structural studies. Crystal structures of archaeal exosomes bound to RNA and of the corresponding nine-subunit human exosome cor...

journal_title：Current opinion in structural biology

authors： Lorentzen E,Basquin J,Conti E

更新日期：2008-12-01 00:00:00

abstract：:Helical filaments were the first structures to be reconstructed in three dimensions from electron microscopic images, and continue to be extensively studied due to the large number of such helical polymers found in biology. In principle, a single image of a helical polymer provides all of the different projections nee...

journal_title：Current opinion in structural biology

authors： Egelman EH

更新日期：2007-10-01 00:00:00

abstract：:Electron cryo-microscopy (cryoEM) is used to determine structures of biological molecules, including multi-protein complexes. Maps at better than 3.0Å resolution are relatively straightforward to interpret since atomic models of proteins and nucleic acids can be built directly. Still, these resolutions are often diffi...

journal_title：Current opinion in structural biology

authors： Casañal A,Shakeel S,Passmore LA

更新日期：2019-10-01 00:00:00

abstract：:X-ray radiation damage to cryocooled ( approximately 100 K) macromolecular crystals has emerged as a general problem, especially since the advent of third generation synchrotron undulator sources. Interest in understanding the physical and chemical phenomena behind the observed effects is growing rapidly. The specific...

journal_title：Current opinion in structural biology

authors： Ravelli RB,Garman EF

更新日期：2006-10-01 00:00:00

abstract：:Histones are highly positively charged proteins that wrap our genome. Their surface properties also make them prone to nonspecific interactions and aggregation. A class of proteins known as histone chaperones is dedicated to safeguard histones by aiding their proper incorporation into nucleosomes. Histone chaperones f...

journal_title：Current opinion in structural biology

authors： Hondele M,Ladurner AG

更新日期：2011-12-01 00:00:00

abstract：:Computational protein design - the search for amino acids that adopt defined structures and functions - has achieved significant successes in recent years. Design is often approached from the perspective of targeting the design objective one structure-sequence pair at a time. Here we summarize studies that take an alt...

journal_title：Current opinion in structural biology

authors： Friedland GD,Kortemme T

更新日期：2010-06-01 00:00:00

abstract：:Historically it has been virtually impossible to experimentally determine the contribution of residual protein entropy to fundamental protein activities such as the binding of ligands. Recent progress has illuminated the possibility of employing NMR relaxation methods to quantitatively determine the role of changes in...

journal_title：Current opinion in structural biology

authors： Wand AJ

更新日期：2013-02-01 00:00:00

abstract：:Since user-friendly atomic force microscopes came onto the market a few years ago, scientists have explored the response of many proteins to applied force. This field has now matured beyond the phenomenological with exciting recent developments, particularly with regards to research into biological questions. For exam...

journal_title：Current opinion in structural biology

authors： Forman JR,Clarke J

更新日期：2007-02-01 00:00:00

abstract：:Force can drive conformational changes in proteins, as well as modulate their stability and the affinity of their complexes, allowing a mechanical input to be converted into a biochemical output. These properties have been utilised by nature and force is now recognised to be widely used at the cellular level. The effe...

journal_title：Current opinion in structural biology

authors： Chen Y,Radford SE,Brockwell DJ

更新日期：2015-02-01 00:00:00

abstract：:Neutron diffraction provides different information from X-ray diffraction, because neutrons are scattered by atomic nuclei, whereas X-rays are scattered by electrons. One of the key advantages of neutron crystallography is the ability to visualize hydrogen and deuterium atoms, making it possible to observe the protona...

journal_title：Current opinion in structural biology

authors： Nakamura A,Ishida T,Samejima M,Igarashi K

更新日期：2016-10-01 00:00:00

abstract：:ATP- and NAD(+)-dependent DNA ligases, ATP-dependent RNA ligases and GTP-dependent mRNA capping enzymes comprise a superfamily of proteins that catalyze nucleotidyl transfer to polynucleotide 5' ends via covalent enzyme-(lysyl-N)-NMP intermediates. The superfamily is defined by five peptide motifs that line the nucleo...

journal_title：Current opinion in structural biology

authors： Shuman S,Lima CD

更新日期：2004-12-01 00:00:00