Abstract:
:Over recent years, nuclear magnetic resonance (NMR) spectroscopy has developed into a powerful mechanistic tool for the investigation of G protein-coupled receptors (GPCRs). NMR provides insights which underpin the dynamic nature of these important receptors and reveals experimental evidence for a complex conformational energy landscape that is explored during receptor activation resulting in signalling. NMR studies have highlighted both the dynamic properties of different receptor states as well as the exchange pathways and intermediates formed during activation, extending the static view of GPCRs obtained from other techniques. NMR studies can be undertaken in realistic membrane-like phospholipid environments and an ever-increasing choice of labelling strategies provides comprehensive, receptor-wide information. Combined with other structural methods, NMR is contributing to our understanding of allosteric signal propagation and the interaction of GPCRs with intracellular binding partners (IBP), crucial to explaining cellular signalling.
journal_name
Curr Opin Struct Bioljournal_title
Current opinion in structural biologyauthors
Bostock MJ,Solt AS,Nietlispach Ddoi
10.1016/j.sbi.2019.03.030subject
Has Abstractpub_date
2019-08-01 00:00:00pages
145-156eissn
0959-440Xissn
1879-033Xpii
S0959-440X(19)30028-4journal_volume
57pub_type
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