Cyanobacteriochromes: photoreceptors covering the entire UV-to-visible spectrum.

abstract：:The radical intermediates generated during the catalytic cycles of adenosylcobalamin-dependent enzymes occur in pairs. The positions of radicals residing on the cofactor, substrate or protein, relative to the position of the low-spin Co(2+) from the cob(II)alamin intermediate, can be extracted from electron paramagnet...

journal_title：Current opinion in structural biology

authors： Reed GH,Mansoorabadi SO

更新日期：2003-12-01 00:00:00

abstract：:Few biological macromolecular complexes exhibit the combination of massive size and hierarchical, symmetrical architecture embodied in icosahedral viruses. X-ray crystallography, electron cryomicroscopy and small-angle X-ray scattering provide complementary approaches to studying these remarkable structures. Through a...

journal_title：Current opinion in structural biology

authors： Lee KK,Johnson JE

更新日期：2003-10-01 00:00:00

abstract：:Electron cryo-microscopy (cryoEM) is used to determine structures of biological molecules, including multi-protein complexes. Maps at better than 3.0Å resolution are relatively straightforward to interpret since atomic models of proteins and nucleic acids can be built directly. Still, these resolutions are often diffi...

journal_title：Current opinion in structural biology

authors： Casañal A,Shakeel S,Passmore LA

更新日期：2019-10-01 00:00:00

abstract：:Historically it has been virtually impossible to experimentally determine the contribution of residual protein entropy to fundamental protein activities such as the binding of ligands. Recent progress has illuminated the possibility of employing NMR relaxation methods to quantitatively determine the role of changes in...

journal_title：Current opinion in structural biology

authors： Wand AJ

更新日期：2013-02-01 00:00:00

abstract：:Recent studies from several laboratories have provided evidence that cell surface complex carbohydrates play key roles in the regulation of developmentally relevant signal transduction events. The demonstration that Fringe, a known modifier of Notch function, is a fucose-specific N-acetylglucosaminyltransferase provid...

journal_title：Current opinion in structural biology

authors： Haltiwanger RS

更新日期：2002-10-01 00:00:00

abstract：:In all contemporary organisms, the active site of the ribosome--the peptidyl transferase center--catalyzes two distinct reactions, peptide bond formation between peptidyl-tRNA and aminoacyl-tRNA as well as the hydrolysis of peptidyl-tRNA with the help of a release factor. However, when provided with appropriate substr...

journal_title：Current opinion in structural biology

authors： Rodnina MV

更新日期：2013-08-01 00:00:00

abstract：:Solid-state NMR (ssNMR) offers insight into the formation of protein complexes and ligand binding for a large range of molecular sizes and binding affinities. Recent instrumental and methodological progress has enabled novel possibilities for using multi-dimensional ssNMR to study molecular 3D structures and interacti...

journal_title：Current opinion in structural biology

authors： Baldus M

更新日期：2006-10-01 00:00:00

abstract：:Mechanisms for making and breaking the heme b cofactor (heme) are more diverse than previously expected. Biosynthetic pathways have diverged at least twice along taxonomic lines, reflecting differences in membrane organization and O2 utilization among major groups of organisms. At least three families of heme degradas...

journal_title：Current opinion in structural biology

authors： Celis AI,DuBois JL

更新日期：2019-12-01 00:00:00

abstract：:Channel-forming bacterial toxins undergo a series of remarkable changes in solubility, oligomerization state, structure and dynamics during the processes of membrane binding, assembly, membrane insertion and channel formation. Recent high-resolution crystal structures of channel-forming toxins, in both water-soluble a...

journal_title：Current opinion in structural biology

authors： Gouaux E

更新日期：1997-08-01 00:00:00

abstract：:Fucose is a common terminal modification on protein and lipid glycans. Fucose can also be directly linked to protein via an O-linkage to Serine or Threonine residues located within consensus sequences contained in Epidermal Growth Factor-like (EGF) repeats and Thrombospondin Type 1 Repeats (TSRs). In this context, fuc...

journal_title：Current opinion in structural biology

authors： Holdener BC,Haltiwanger RS

更新日期：2019-06-01 00:00:00

abstract：:The winged helix proteins constitute a subfamily within the large ensemble of helix-turn-helix proteins. Since the discovery of the winged helix/fork head motif in 1993, a large number of topologically related proteins with diverse biological functions have been characterized by X-ray crystallography and solution NMR ...

journal_title：Current opinion in structural biology

authors： Gajiwala KS,Burley SK

更新日期：2000-02-01 00:00:00

abstract：:The surfaces of all microbes are 'sugar coated' with molecules such as lipopolysaccharides in Gram-negative bacteria, capsular polysaccharides in bacteria, lipoarabinomannans in mycobacteria and lipophosphoglycan in Leishmania. The basic structures of these glycoconjugates are known and, in the case of pathogens, they...

journal_title：Current opinion in structural biology

authors： Mengeling BJ,Turco SJ

更新日期：1998-10-01 00:00:00

abstract：:The three-dimensional structures of several biotin-binding proteins are now known, giving insights into the molecular architecture of the binding sites for biotin. In combination with biochemical and computational approaches, these structural insights provide the basis for our present understanding of biotin-protein i...

journal_title：Current opinion in structural biology

authors： Lindqvist Y,Schneider G

更新日期：1996-12-01 00:00:00

abstract：:Akt1-3 (Akt) are a subset of the AGC protein Ser/Thr kinase family and play important roles in cell growth, metabolic regulation, cancer, and other diseases. We describe some of the roles of Akt in cell signaling and the biochemical and structural mechanisms of the regulation of Akt catalysis by the phospholipid PIP3 ...

journal_title：Current opinion in structural biology

authors： Cole PA,Chu N,Salguero AL,Bae H

更新日期：2019-12-01 00:00:00

abstract：:High-throughput interaction discovery initiatives have revealed the existence of hundreds of multiprotein complexes whose functions are regulated through thousands of protein-protein interactions (PPIs). However, the structural details of these interactions, often necessary to understand their function, are only avail...

journal_title：Current opinion in structural biology

authors： Stein A,Mosca R,Aloy P

更新日期：2011-04-01 00:00:00

abstract：:Toll-like receptors (TLRs) activate the innate immune system in response to invading pathogens. Although nucleic acids are one of the principal TLR ligands, they are not inherently pathogen-specific and, thus, carry the risk of triggering autoimmunity. There are multiple unique regulatory mechanisms aimed at preventin...

journal_title：Current opinion in structural biology

authors： Shimizu T

更新日期：2017-12-01 00:00:00

abstract：:Synapses are cell adhesion structures specialized for signal transmission between neurons. At the synapse, presynaptic and postsynaptic terminals of neurons are functionally connected but spatially separated and form a cleft. Membrane receptor-like cell adhesion molecules and secreted proteins in the synaptic cleft (s...

journal_title：Current opinion in structural biology

authors： Yamagata A,Fukai S

更新日期：2019-02-01 00:00:00

abstract：:The accelerated elucidation of three-dimensional structures of protein complexes, both natural and designed, is providing new examples of large supramolecular assemblies with intriguing shapes. Those with high symmetry - based on the geometries of the Platonic solids - are particularly notable as their innately closed...

journal_title：Current opinion in structural biology

authors： Cannon KA,Ochoa JM,Yeates TO

更新日期：2019-04-01 00:00:00

abstract：:Electrostatics plays a major role in the stabilization and function of biomolecules; as such, it remains a major focus of theoretical and computational studies of macromolecules. Electrostatic interactions are long range, and strongly dependent on the solvent and ions surrounding the biomolecule under study. During th...

journal_title：Current opinion in structural biology

authors： Koehl P

更新日期：2006-04-01 00:00:00

abstract：:Specialized metabolic enzymes and metabolite diversity evolve through a variety of mechanisms including promiscuity, changes in substrate specificity, modifications of gene expression and gene duplication. For example, gene duplication and substrate binding site changes led to the evolution of the glucosinolate biosyn...

journal_title：Current opinion in structural biology

authors： Leong BJ,Last RL

更新日期：2017-12-01 00:00:00

abstract：:TolC and its many homologues comprise an alpha-helical transperiplasmic tunnel embedded in the bacterial outer membrane by a contiguous beta-barrel channel, providing a large exit duct for diverse substrates. The 'channel-tunnel' is closed at its periplasmic entrance, but can be opened by an 'iris-like' mechanism when...

journal_title：Current opinion in structural biology

authors： Koronakis V,Andersen C,Hughes C

更新日期：2001-08-01 00:00:00

abstract：:Due to the contribution of drug-target binding kinetics to drug efficacy, there is a high level of interest in developing methods to predict drug-target binding kinetic parameters. During the review period, a wide range of enhanced sampling molecular dynamics simulation-based methods has been developed for computing d...

journal_title：Current opinion in structural biology

authors： Nunes-Alves A,Kokh DB,Wade RC

更新日期：2020-10-01 00:00:00

abstract：:CCA-adding enzymes (tRNA nucleotidyltransferases) are responsible for the maturation or repair of the functional 3' end of tRNAs. These enzymes are remarkable because they polymerize the essential nucleotides CCA onto the 3' terminus of tRNA precursors without using a nucleic acid template. Recent crystal structures, ...

journal_title：Current opinion in structural biology

authors： Xiong Y,Steitz TA

更新日期：2006-02-01 00:00:00

abstract：:Allostery plays important roles in many biological processes. Although all non-fibrous proteins may be allosteric, currently only a limited number of allosteric proteins are known. How allosteric regulation depends on protein topology and what are the preferred folds in allosteric proteins need to be explored. Alloste...

journal_title：Current opinion in structural biology

authors： Xie J,Lai L

更新日期：2020-06-01 00:00:00

abstract：:Force can drive conformational changes in proteins, as well as modulate their stability and the affinity of their complexes, allowing a mechanical input to be converted into a biochemical output. These properties have been utilised by nature and force is now recognised to be widely used at the cellular level. The effe...

journal_title：Current opinion in structural biology

authors： Chen Y,Radford SE,Brockwell DJ

更新日期：2015-02-01 00:00:00

abstract：:Protein allostery is a vitally important protein function that has proven to be a vexing problem to understand at the molecular level. Allosteric communication is a hallmark of many protein functions. However, despite more than four decades of study the details regarding allosteric communication in protein systems are...

journal_title：Current opinion in structural biology

authors： Herde ZD,Short AE,Kay VE,Huang BD,Realff MJ,Wilson CJ

更新日期：2020-08-01 00:00:00

abstract：:The most important advances in the field of genome annotation over the past two years involve the use of cDNA sequences, protein structures and gene expression data to predict genes. These types of information not only improve gene identification, but they also give insights into variation in gene structure and functi...

journal_title：Current opinion in structural biology

authors： Gaasterland T,Oprea M

更新日期：2001-06-01 00:00:00

abstract：:Protein biogenesis integrates multiple finely regulated mechanisms, ensuring nascent polypeptide chains are correctly enzymatically processed, targeted to membranes and folded to native structure. Recent studies show that the cellular translation machinery serves as hub that coordinates the maturation events in space ...

journal_title：Current opinion in structural biology

authors： Gloge F,Becker AH,Kramer G,Bukau B

更新日期：2014-02-01 00:00:00

abstract：:Large ribozymes fold on a 'glacial' timescale compared to the folding of their protein counterparts. The sluggish folding exhibited by large RNAs results from the formation of kinetically trapped, misfolded intermediates, which are nonessential features of the folding mechanism. Newly developed mutant ribozymes that a...

journal_title：Current opinion in structural biology

authors： Treiber DK,Williamson JR

更新日期：1999-06-01 00:00:00

abstract：:Molecular flexibility over a wide time range is of central importance to the function of many proteins, both soluble and membrane. Revealing the modes of flexibility, their amplitudes, and time scales under physiological conditions is the challenge for spectroscopic methods, one of which is site-directed spin labeling...

journal_title：Current opinion in structural biology

authors： Hubbell WL,López CJ,Altenbach C,Yang Z

更新日期：2013-10-01 00:00:00