Exposing the kinetic traps in RNA folding.

abstract：:Eukaryotic transcriptional coactivators are multi-subunit complexes that both modify chromatin and recognize histone modifications. Until recently, structural information on these large complexes has been limited to isolated enzymatic domains or chromatin-binding motifs. This review summarizes recent structural studie...

journal_title：Current opinion in structural biology

authors： Samara NL,Wolberger C

更新日期：2011-12-01 00:00:00

abstract：:ATP synthases are rotary enzymes found in bacteria, chloroplasts, and mitochondria. These complexes produce the majority of cellular ATP in aerobic cells using energy from the transmembrane proton motive force established by the electron transport chain. In mitochondria, dimeric ATP synthase is essential for formation...

journal_title：Current opinion in structural biology

authors： Guo H,Rubinstein JL

更新日期：2018-10-01 00:00:00

abstract：:Helical filaments were the first structures to be reconstructed in three dimensions from electron microscopic images, and continue to be extensively studied due to the large number of such helical polymers found in biology. In principle, a single image of a helical polymer provides all of the different projections nee...

journal_title：Current opinion in structural biology

authors： Egelman EH

更新日期：2007-10-01 00:00:00

abstract：:For many protein families, such as serine proteinases or serine proteinase inhibitors, the family assignment predicts reactivity only in general terms. Both detailed specificity and quantitative reactivity are lacking. We believe that, for many such protein families, algorithms can be devised by defining the subset of...

journal_title：Current opinion in structural biology

authors： Laskowski M Jr,Qasim MA,Yi Z

更新日期：2003-02-01 00:00:00

abstract：:Molecular dynamics simulations of membrane proteins are making rapid progress, because of new high-resolution structures, advances in computer hardware and atomistic simulation algorithms, and the recent introduction of coarse-grained models for membranes and proteins. In addition to several large ion channel simulati...

journal_title：Current opinion in structural biology

authors： Lindahl E,Sansom MS

更新日期：2008-08-01 00:00:00

abstract：:Current research into the morphology of lipid membranes focuses on three areas: first, the transformations and fluctuations of the shape of freely suspended vesicles; second, the morphology of membranes that experience mutual interactions or external forces arising, for example, from a macroscopic surface or from opti...

journal_title：Current opinion in structural biology

authors： Lipowsky R

更新日期：1995-08-01 00:00:00

abstract：:Due to the contribution of drug-target binding kinetics to drug efficacy, there is a high level of interest in developing methods to predict drug-target binding kinetic parameters. During the review period, a wide range of enhanced sampling molecular dynamics simulation-based methods has been developed for computing d...

journal_title：Current opinion in structural biology

authors： Nunes-Alves A,Kokh DB,Wade RC

更新日期：2020-10-01 00:00:00

abstract：:H+ transporting, reversible F-type ATPases (ATP synthases) and V-type ATPases share major structural features and function by similar mechanisms. Recent structural and genetic experiments provide new insights into the organization of the transmembrane and coupling sectors of the enzymes and the molecular mechanics of ...

journal_title：Current opinion in structural biology

authors： Fillingame RH

更新日期：1996-08-01 00:00:00

abstract：:Protein design efforts are beginning to yield molecules with many of the properties of natural proteins. Such experiments are informed by and contribute to our understanding of the sequence determinants of protein folding and stability. The most important design elements seem to be the proper placement of hydrophobic ...

journal_title：Current opinion in structural biology

authors： Cordes MH,Davidson AR,Sauer RT

更新日期：1996-02-01 00:00:00

abstract：:Ribosome recycling usually occurs after canonical termination triggered by a stop codon. Additionally, ribosomes that are stalled by aberrant mRNAs need to be recognized and subsequently recycled. In eukaryotes and archaea, the factors involved in canonical termination and ribosome rescue are structurally and function...

journal_title：Current opinion in structural biology

authors： Franckenberg S,Becker T,Beckmann R

更新日期：2012-12-01 00:00:00

abstract：:Histones are highly positively charged proteins that wrap our genome. Their surface properties also make them prone to nonspecific interactions and aggregation. A class of proteins known as histone chaperones is dedicated to safeguard histones by aiding their proper incorporation into nucleosomes. Histone chaperones f...

journal_title：Current opinion in structural biology

authors： Hondele M,Ladurner AG

更新日期：2011-12-01 00:00:00

abstract：:Rapid advances in the cloning and expression of glycosyltransferase genes, especially from bacteria, could open the way to overcoming difficulties in the mass production of oligosaccharides. The large-scale production of oligosaccharides using either glycosyltransferases isolated from engineered microorganisms or whol...

journal_title：Current opinion in structural biology

authors： Endo T,Koizumi S

更新日期：2000-10-01 00:00:00

abstract：:The first high-resolution structure of a P-type ATPase, that of the Ca(2+)-ATPase of skeletal muscle sarcoplasmic reticulum, was published in 2000. This structure has provided many clues to how the Ca(2+)-ATPase might work, but no complete answers. The Ca(2+)-ATPase structure reveals no clear pathway from the cytoplas...

journal_title：Current opinion in structural biology

authors： Lee AG

更新日期：2002-08-01 00:00:00

abstract：:The small multidrug resistance proteins constitute a family of bacterial antiporters that confer multidrug resistance by H(+)-linked drug efflux across the bacterial cytoplasmic membrane. The structure of EmrE, the family archetype, has been determined by electron crystallography and shows that EmrE in the membrane is...

journal_title：Current opinion in structural biology

authors： Tate CG

更新日期：2006-08-01 00:00:00

abstract：:Transition states in protein folding are being analyzed experimentally and by computer simulation to reveal the sequence of events in the folding and unfolding of proteins at high resolution. Results of this analysis have enabled the reconstruction of folding pathways in vitro and open the prospect of analyzing pathwa...

journal_title：Current opinion in structural biology

authors： Fersht AR

更新日期：1995-02-01 00:00:00

abstract：:Recently solved structures and proposed models have helped to reveal the structural characteristics of the beta-propeller fold, as well as the features that contribute to its high rigidity and stability. Possible strategies for identifying beta-propeller proteins in newly characterised sequences are helping to overcom...

journal_title：Current opinion in structural biology

authors： Fülöp V,Jones DT

更新日期：1999-12-01 00:00:00

abstract：:2-Oxoacid:ferredoxin oxidoreductases (OFORs) are essential enzymes in microbial one-carbon metabolism. They use thiamine pyrophosphate to reversibly cleave carbon-carbon bonds, generating low potential (∼-500mV) electrons. Crystallographic analysis of a recently discovered OFOR, an oxalate oxidoreductase (OOR), has pr...

journal_title：Current opinion in structural biology

authors： Gibson MI,Chen PY,Drennan CL

更新日期：2016-12-01 00:00:00

abstract：:Aromatic polyketides are secondary metabolites that afford some of the most common antibiotics and anti-cancer drugs currently in clinical use. Not least because of their medical importance, the biosynthesis of these compounds has attracted considerable interest during the past few years; important advances have been ...

journal_title：Current opinion in structural biology

authors： Schneider G

更新日期：2005-12-01 00:00:00

abstract：:The surfaces of all microbes are 'sugar coated' with molecules such as lipopolysaccharides in Gram-negative bacteria, capsular polysaccharides in bacteria, lipoarabinomannans in mycobacteria and lipophosphoglycan in Leishmania. The basic structures of these glycoconjugates are known and, in the case of pathogens, they...

journal_title：Current opinion in structural biology

authors： Mengeling BJ,Turco SJ

更新日期：1998-10-01 00:00:00

abstract：:Protein-RNA interactions play essential roles in gene regulation and RNA metabolism. While high-resolution structures have revealed principles of RNA recognition by individual RNA binding domains (RBDs), the presence of multiple RBDs in many eukaryotic proteins suggests additional modes of RNA recognition by combinati...

journal_title：Current opinion in structural biology

authors： Mackereth CD,Sattler M

更新日期：2012-06-01 00:00:00

abstract：:Metal-binding sites have been engineered into both de novo designed and naturally occurring proteins. Although the redesign of existing metal-binding sites in naturally occurring proteins still offers the most promise for a successful design, the more challenging goal of engineering metal-binding sites in de novo desi...

journal_title：Current opinion in structural biology

authors： Lu Y,Valentine JS

更新日期：1997-08-01 00:00:00

abstract：:The most important advances in the field of genome annotation over the past two years involve the use of cDNA sequences, protein structures and gene expression data to predict genes. These types of information not only improve gene identification, but they also give insights into variation in gene structure and functi...

journal_title：Current opinion in structural biology

authors： Gaasterland T,Oprea M

更新日期：2001-06-01 00:00:00

abstract：:Centrioles are microtubule-based structures in eukaryotic cells. From organizing the microtubule cytoskeleton during interphase to focusing the mitotic spindle during mitosis, centrioles are busy at all stages of the cell cycle. One crucial interphase function of centrioles is to assemble cilia, microtubular projectio...

journal_title：Current opinion in structural biology

authors： Kumar D,Reiter J

更新日期：2020-11-04 00:00:00

abstract：:Alternatively spliced protein segments tend to be intrinsically disordered and contain linear interaction motifs and/or post-translational modification sites. An emerging concept is that differential inclusion of such disordered segments can mediate new protein interactions, and hence change the context in which the b...

journal_title：Current opinion in structural biology

authors： Buljan M,Chalancon G,Dunker AK,Bateman A,Balaji S,Fuxreiter M,Babu MM

更新日期：2013-06-01 00:00:00

abstract：:Connecting dynamics to structural data from diverse experimental sources, molecular dynamics simulations permit the exploration of biological phenomena in unparalleled detail. Advances in simulations are moving the atomic resolution descriptions of biological systems into the million-to-billion atom regime, in which n...

journal_title：Current opinion in structural biology

authors： Perilla JR,Goh BC,Cassidy CK,Liu B,Bernardi RC,Rudack T,Yu H,Wu Z,Schulten K

更新日期：2015-04-01 00:00:00

abstract：:Cells are equipped with cosolvents that modulate protein folding and aggregation to withstand water stress. The effect of cosolvents on the aggregation rates depends on whether the polypeptide sequence is an intrinsically disordered protein (IDP) or can fold into a specific native structure. Cosolvents, which act as d...

journal_title：Current opinion in structural biology

authors： Reddy G,Muttathukattil AN,Mondal B

更新日期：2020-02-01 00:00:00

abstract：:There are now many successful examples of the design of new ligands based on knowledge of target protein structures. In most cases those ligands are unsuitable as drugs because of problems of toxicity, stability or bioavailability. The past twelve months have also seen the description of the structures of many protein...

journal_title：Current opinion in structural biology

authors： Colman PM

更新日期：1994-12-01 00:00:00

abstract：:Molecular dynamics simulations allow for atomic-level characterization of biomolecular processes such as the conformational transitions associated with protein function. The computational demands of such simulations, however, have historically prevented them from reaching the microsecond and greater timescales on whic...

journal_title：Current opinion in structural biology

authors： Klepeis JL,Lindorff-Larsen K,Dror RO,Shaw DE

更新日期：2009-04-01 00:00:00

abstract：:Biological macromolecules have evolved over billions of years to function inside cells, so it is not surprising that researchers studying the properties of such molecules, either in extracts or in purified form, take care to control factors that reflect the intracellular environment, such as pH, ionic strength and com...

journal_title：Current opinion in structural biology

authors： Ellis RJ

更新日期：2001-02-01 00:00:00

abstract：:Recent advances in ab initio direct methods have enabled the solution of crystal structures of small proteins from native X-ray data alone, that is, without the use of fragments of known structure or the need to prepare heavy-atom or selenomethionine derivatives, provided that the data are available to atomic resoluti...

journal_title：Current opinion in structural biology

authors： Usón I,Sheldrick GM

更新日期：1999-10-01 00:00:00