Additivity-based prediction of equilibrium constants for some protein-protein associations.

Abstract:

:For many protein families, such as serine proteinases or serine proteinase inhibitors, the family assignment predicts reactivity only in general terms. Both detailed specificity and quantitative reactivity are lacking. We believe that, for many such protein families, algorithms can be devised by defining the subset of n functionally important sequence positions, making the 19n possible single mutants and measuring their reactivity. Given the assumption that the contributions of the n positions are additive, the reactivities of the 20(n) variants can be predicted. This is illustrated by an almost complete algorithm for the Kazal family of protein inhibitors of serine proteinases.

journal_name

Curr Opin Struct Biol

authors

Laskowski M Jr,Qasim MA,Yi Z

doi

10.1016/s0959-440x(03)00013-7

subject

Has Abstract

pub_date

2003-02-01 00:00:00

pages

130-9

issue

1

eissn

0959-440X

issn

1879-033X

pii

S0959440X03000137

journal_volume

13

pub_type

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