Abstract:
:For many protein families, such as serine proteinases or serine proteinase inhibitors, the family assignment predicts reactivity only in general terms. Both detailed specificity and quantitative reactivity are lacking. We believe that, for many such protein families, algorithms can be devised by defining the subset of n functionally important sequence positions, making the 19n possible single mutants and measuring their reactivity. Given the assumption that the contributions of the n positions are additive, the reactivities of the 20(n) variants can be predicted. This is illustrated by an almost complete algorithm for the Kazal family of protein inhibitors of serine proteinases.
journal_name
Curr Opin Struct Bioljournal_title
Current opinion in structural biologyauthors
Laskowski M Jr,Qasim MA,Yi Zdoi
10.1016/s0959-440x(03)00013-7subject
Has Abstractpub_date
2003-02-01 00:00:00pages
130-9issue
1eissn
0959-440Xissn
1879-033Xpii
S0959440X03000137journal_volume
13pub_type
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