Advances in direct methods for protein crystallography.

abstract：:NMR relaxation experiments of isotopically labeled proteins provide a wealth of information on reorientational global and local dynamics on nanosecond and subnanosecond timescales for folded and nonfolded proteins in solution. Recent methodological advances in the interpretation of relaxation data have led to a better...

journal_title：Current opinion in structural biology

authors： Brüschweiler R

更新日期：2003-04-01 00:00:00

abstract：:Significant progress has been made over the past several years on structural studies of the eukaryotic translation initiation factors that facilitate the assembly of a translation-competent ribosome at the initiation codon of an mRNA. These structural studies have revealed the repeated use of a set of common structura...

journal_title：Current opinion in structural biology

authors： Sonenberg N,Dever TE

更新日期：2003-02-01 00:00:00

abstract：:RNA motifs are directed and ordered stacked arrays of non-Watson-Crick base pairs forming distinctive foldings of the phosphodiester backbones of the interacting RNA strands. They correspond to the 'loops' - hairpin, internal and junction - that intersperse the Watson-Crick two-dimensional helices as seen in two-dimen...

journal_title：Current opinion in structural biology

authors： Leontis NB,Westhof E

更新日期：2003-06-01 00:00:00

abstract：:The major component of bacterial cell wall is peptidoglycan (PG), a complex polymer formed by long glycan chains cross-linked by peptide stems. PG is in constant equilibrium requiring well-orchestrated coordination between synthesis and degradation. The resulting cell-wall fragments can be recycled, act as messengers ...

journal_title：Current opinion in structural biology

authors： Alcorlo M,Martínez-Caballero S,Molina R,Hermoso JA

更新日期：2017-06-01 00:00:00

abstract：:Functional conformational changes of proteins can facilitate numerous biological events in cells. The Markov state model (MSM) built from molecular dynamics simulations provide a powerful approach to study them. We here introduce a protocol that is tailor-made for constructing MSMs to study the functional conformation...

journal_title：Current opinion in structural biology

authors： Wang X,Unarta IC,Cheung PP,Huang X

更新日期：2020-10-27 00:00:00

abstract：:Over 5000 halogenated natural products have been reported so far, many of these arising from the marine environment. The introduction of a halogen into a molecule can significantly impact its bioavailability and bioactivity. More recently enzymatic halogenation has been used to enable late stage functionalisation thro...

journal_title：Current opinion in structural biology

authors： Ludewig H,Molyneux S,Ferrinho S,Guo K,Lynch R,Gkotsi DS,Goss RJ

更新日期：2020-12-01 00:00:00

abstract：:In vitro selection experiments are now routinely used to identify functional nucleic acid residues and structures, and have become a tool for studying molecular recognition, molecular biology, and molecular evolution. Technical innovations that have been made during the past year include the use of modified monomers t...

journal_title：Current opinion in structural biology

authors： Uphoff KW,Bell SD,Ellington AD

更新日期：1996-06-01 00:00:00

abstract：:Biological macromolecules have evolved over billions of years to function inside cells, so it is not surprising that researchers studying the properties of such molecules, either in extracts or in purified form, take care to control factors that reflect the intracellular environment, such as pH, ionic strength and com...

journal_title：Current opinion in structural biology

authors： Ellis RJ

更新日期：2001-02-01 00:00:00

abstract：:The bacterial cell wall is a complex polymeric structure with essential roles in defence, survival and pathogenesis. Common to both Gram-positive and Gram-negative bacteria is the mesh-like peptidoglycan sacculus that surrounds the outer leaflet of the cytoplasmic membrane. Recent crystallographic studies of enzymes t...

journal_title：Current opinion in structural biology

authors： Caveney NA,Li FK,Strynadka NC

更新日期：2018-12-01 00:00:00

abstract：:Force spectroscopy has developed into an indispensable tool for studying folding and binding of proteins on a single molecule level in real time. Design of the pulling geometry allows tuning the reaction coordinate in a very precise manner. Many recent experiments have taken advantage of this possibility and have prov...

journal_title：Current opinion in structural biology

authors： Zoldák G,Rief M

更新日期：2013-02-01 00:00:00

abstract：:Complementary developments in comparative genomics, protein structure determination and in-depth comparison of protein sequences and structures have provided a better understanding of the prevailing trends in the emergence and diversification of protein domains. The investigation of deep relationships among different ...

journal_title：Current opinion in structural biology

authors： Aravind L,Mazumder R,Vasudevan S,Koonin EV

更新日期：2002-06-01 00:00:00

abstract：:Conformational dynamics is a hallmark of diverse non-coding RNA functions. During these functional processes, RNA molecules almost ubiquitously undergo conformational transitions that are tuned to meet distinct structural and kinetic requirements for proper function. A complete mechanistic understanding of RNA functio...

journal_title：Current opinion in structural biology

authors： Zhao B,Zhang Q

更新日期：2015-02-01 00:00:00

abstract：:Bacterial transcription initiation is controlled by sigma factors, the RNA polymerase (RNAP) subunits responsive for promoter specificity. While the primary sigma factor ensures the bulk of transcription during growth, a major strategy used by bacteria to regulate gene expression consists of modifying the RNAP promote...

journal_title：Current opinion in structural biology

authors： Campagne S,Allain FH,Vorholt JA

更新日期：2015-02-01 00:00:00

abstract：:The encounter complex of two proteins is a dynamic intermediate state that guides proteins to their binding site, thus enhancing the rate of complex formation. It is particularly useful for complexes that must balance a biological requirement for high turnover with the need for specific binding, such as electron trans...

journal_title：Current opinion in structural biology

authors： Schilder J,Ubbink M

更新日期：2013-12-01 00:00:00

abstract：:Helical filaments were the first structures to be reconstructed in three dimensions from electron microscopic images, and continue to be extensively studied due to the large number of such helical polymers found in biology. In principle, a single image of a helical polymer provides all of the different projections nee...

journal_title：Current opinion in structural biology

authors： Egelman EH

更新日期：2007-10-01 00:00:00

abstract：:Given the increasing interest in protein-protein interactions, the prediction of these interactions from sequence and structural information has become a booming activity. CAPRI, the community-wide experiment for assessing blind predictions of protein-protein interactions, is playing an important role in fostering pro...

journal_title：Current opinion in structural biology

authors： Wodak SJ,Méndez R

更新日期：2004-04-01 00:00:00

abstract：:Large ribozymes fold on a 'glacial' timescale compared to the folding of their protein counterparts. The sluggish folding exhibited by large RNAs results from the formation of kinetically trapped, misfolded intermediates, which are nonessential features of the folding mechanism. Newly developed mutant ribozymes that a...

journal_title：Current opinion in structural biology

authors： Treiber DK,Williamson JR

更新日期：1999-06-01 00:00:00

abstract：:The small multidrug resistance proteins constitute a family of bacterial antiporters that confer multidrug resistance by H(+)-linked drug efflux across the bacterial cytoplasmic membrane. The structure of EmrE, the family archetype, has been determined by electron crystallography and shows that EmrE in the membrane is...

journal_title：Current opinion in structural biology

authors： Tate CG

更新日期：2006-08-01 00:00:00

abstract：:The spliceosome executes eukaryotic precursor messenger RNA (pre-mRNA) splicing to remove noncoding introns through two sequential transesterification reactions, branching and exon ligation. The fidelity of this process is based on the recognition of the conserved sequences in the intron and dynamic compositional and ...

journal_title：Current opinion in structural biology

authors： Wan R,Bai R,Shi Y

更新日期：2019-12-01 00:00:00

abstract：:X-ray radiation damage to cryocooled ( approximately 100 K) macromolecular crystals has emerged as a general problem, especially since the advent of third generation synchrotron undulator sources. Interest in understanding the physical and chemical phenomena behind the observed effects is growing rapidly. The specific...

journal_title：Current opinion in structural biology

authors： Ravelli RB,Garman EF

更新日期：2006-10-01 00:00:00

abstract：:The winged helix proteins constitute a subfamily within the large ensemble of helix-turn-helix proteins. Since the discovery of the winged helix/fork head motif in 1993, a large number of topologically related proteins with diverse biological functions have been characterized by X-ray crystallography and solution NMR ...

journal_title：Current opinion in structural biology

authors： Gajiwala KS,Burley SK

更新日期：2000-02-01 00:00:00

abstract：:Alternatively spliced protein segments tend to be intrinsically disordered and contain linear interaction motifs and/or post-translational modification sites. An emerging concept is that differential inclusion of such disordered segments can mediate new protein interactions, and hence change the context in which the b...

journal_title：Current opinion in structural biology

authors： Buljan M,Chalancon G,Dunker AK,Bateman A,Balaji S,Fuxreiter M,Babu MM

更新日期：2013-06-01 00:00:00

abstract：:The eukaryotic genome is hierarchically packaged into chromatin in the nucleus. The organization and dynamics of 30-nm chromatin fibers, which is typically regarded as the secondary structure of chromatin, play a crucial role in regulating DNA accessibility for gene expression. Here we reviewed some recent progresses ...

journal_title：Current opinion in structural biology

authors： Zhu P,Li G

更新日期：2016-02-01 00:00:00

abstract：:Mass spectrometry is becoming a more and more powerful tool for investigating protein complexes. Recent developments, based on different ionization techniques, electrospray, desorption/ionization and others are contributing to the usefulness of MS to describe the organization and structure of large non-covalent assemb...

journal_title：Current opinion in structural biology

authors： Bich C,Zenobi R

更新日期：2009-10-01 00:00:00

abstract：:Current research into the morphology of lipid membranes focuses on three areas: first, the transformations and fluctuations of the shape of freely suspended vesicles; second, the morphology of membranes that experience mutual interactions or external forces arising, for example, from a macroscopic surface or from opti...

journal_title：Current opinion in structural biology

authors： Lipowsky R

更新日期：1995-08-01 00:00:00

abstract：:High-throughput interaction discovery initiatives have revealed the existence of hundreds of multiprotein complexes whose functions are regulated through thousands of protein-protein interactions (PPIs). However, the structural details of these interactions, often necessary to understand their function, are only avail...

journal_title：Current opinion in structural biology

authors： Stein A,Mosca R,Aloy P

更新日期：2011-04-01 00:00:00

abstract：:There are now numerous examples of proteins that are unstructured or only partially structured under physiological conditions and yet are nevertheless functional. Such proteins are especially prevalent in eukaryotes. In many cases, intrinsically disordered proteins adopt folded structures upon binding to their biologi...

journal_title：Current opinion in structural biology

authors： Dyson HJ,Wright PE

更新日期：2002-02-01 00:00:00

abstract：:Eukaryotes possess a variety of translational control mechanisms which function in the surveillance of mRNAs, discriminating between normal and aberrant translation elongation and termination, triggering mRNA decay. The three major evolutionarily conserved eukaryotic pathways are No-Go, Non-Stop and Nonsense-Mediated ...

journal_title：Current opinion in structural biology

authors： Powers KT,Szeto JA,Schaffitzel C

更新日期：2020-12-01 00:00:00

abstract：:Recently, new methods for determining time-evolving structures using infrared analogs of NMR spectroscopy have been introduced that have outstanding potential in structural biology. Already, within the past two years, structures of dipeptides, tripeptides and pentapeptides have been determined on much faster timescale...

journal_title：Current opinion in structural biology

authors： Zanni MT,Hochstrasser RM

更新日期：2001-10-01 00:00:00

abstract：:Many proteins are composed of protein domains, functional units of common descent. Multidomain forms are common in all eukaryotes making up more than half of the proteome and the evolution of novel domain architecture has been accelerated in metazoans. It is also becoming increasingly clear that alternative splicing i...

journal_title：Current opinion in structural biology

authors： Light S,Elofsson A

更新日期：2013-06-01 00:00:00