Winged helix proteins.

abstract：:Protein design efforts are beginning to yield molecules with many of the properties of natural proteins. Such experiments are informed by and contribute to our understanding of the sequence determinants of protein folding and stability. The most important design elements seem to be the proper placement of hydrophobic ...

journal_title：Current opinion in structural biology

authors： Cordes MH,Davidson AR,Sauer RT

更新日期：1996-02-01 00:00:00

abstract：:Significant progress has been made over the past several years on structural studies of the eukaryotic translation initiation factors that facilitate the assembly of a translation-competent ribosome at the initiation codon of an mRNA. These structural studies have revealed the repeated use of a set of common structura...

journal_title：Current opinion in structural biology

authors： Sonenberg N,Dever TE

更新日期：2003-02-01 00:00:00

abstract：:There are now many successful examples of the design of new ligands based on knowledge of target protein structures. In most cases those ligands are unsuitable as drugs because of problems of toxicity, stability or bioavailability. The past twelve months have also seen the description of the structures of many protein...

journal_title：Current opinion in structural biology

authors： Colman PM

更新日期：1994-12-01 00:00:00

abstract：:Newly solved chaperone structures include the thermosome, a group II chaperonin, and a small heat-shock protein. Novel ideas on chaperone mechanism are presented in the forced unfolding hypothesis of GroEL action. Structures of chaperone-pilin complexes reveal the mechanism of chaperone interaction in bacterial pilus ...

journal_title：Current opinion in structural biology

authors： Saibil H

更新日期：2000-04-01 00:00:00

abstract：:Transmembrane proton gradients, known as Proton Motive Force (PMF), serve important physiological functions. In addition to driving ATP synthesis, PMF is harnessed by a large variety of secondary transporters to achieve 'uphill' translocation of specific substrates across the membrane. Proton-coupled secondary transpo...

journal_title：Current opinion in structural biology

authors： Yan N

更新日期：2013-08-01 00:00:00

abstract：:The RNA exosome participates in the degradation and processing of a wide range of RNA molecules. Recent advances in understanding how the exosome is organized and functions largely stem from structural studies. Crystal structures of archaeal exosomes bound to RNA and of the corresponding nine-subunit human exosome cor...

journal_title：Current opinion in structural biology

authors： Lorentzen E,Basquin J,Conti E

更新日期：2008-12-01 00:00:00

abstract：:Investigating the in vitro refolding of proteins that naturally reside in biological membranes is a notoriously difficult task. Biophysical studies on model systems are beginning to provide a sound physical basis for membrane protein folding that should help to alleviate this problem. Highlights of these studies inclu...

journal_title：Current opinion in structural biology

authors： Booth PJ,Curran AR

更新日期：1999-02-01 00:00:00

abstract：:Glycoproteins and protein-carbohydrate complexes in the worldwide Protein Data Bank (wwPDB) can be an excellent source of information for glycoscientists. Unfortunately, a rather large number of errors and inconsistencies is found in the glycan moieties of these 3D structures. This review illustrates frequent problems...

journal_title：Current opinion in structural biology

authors： Joosten RP,Lütteke T

更新日期：2017-06-01 00:00:00

abstract：:Self-compartmentalizing proteases, such as the proteasome and several prokaryotic energy-dependent proteases, are designed to act in the crowded environment of the cell. Proteins destined for degradation are recognized and unfolded by regulatory subcomplexes that invariably contain ATPase modules, before being translo...

journal_title：Current opinion in structural biology

authors： Zwickl P,Baumeister W,Steven A

更新日期：2000-04-01 00:00:00

abstract：:Rapid advances in the cloning and expression of glycosyltransferase genes, especially from bacteria, could open the way to overcoming difficulties in the mass production of oligosaccharides. The large-scale production of oligosaccharides using either glycosyltransferases isolated from engineered microorganisms or whol...

journal_title：Current opinion in structural biology

authors： Endo T,Koizumi S

更新日期：2000-10-01 00:00:00

abstract：:Channel-forming bacterial toxins undergo a series of remarkable changes in solubility, oligomerization state, structure and dynamics during the processes of membrane binding, assembly, membrane insertion and channel formation. Recent high-resolution crystal structures of channel-forming toxins, in both water-soluble a...

journal_title：Current opinion in structural biology

authors： Gouaux E

更新日期：1997-08-01 00:00:00

abstract：:A large body of work has gone into understanding the effect of mutations on protein structure and function. Conventional treatments have involved quantifying the change in stability, activity and relaxation rates of the mutants with respect to the wild-type protein. However, it is now becoming increasingly apparent th...

journal_title：Current opinion in structural biology

authors： Naganathan AN

更新日期：2019-02-01 00:00:00

abstract：:In the prokaryotic CRISPR-Cas adaptive immune systems, a CRISPR RNA (crRNA) assembles with multiple or single Cas proteins to form crRNA ribonucleoprotein (crRNP) effector complexes, responsible for the destruction of invading genetic elements. Although the mechanisms of target recognition and cleavage by the crRNP ef...

journal_title：Current opinion in structural biology

authors： Nishimasu H,Nureki O

更新日期：2017-04-01 00:00:00

abstract：:Salt-dependent electrostatic effects are a major factor in determining the stability, structure, reactivity, and binding behavior of nucleic acids. Increasingly detailed theoretical methods, especially those based on Monte Carlo and Poisson-Boltzmann methodologies, combined with powerful computational algorithms are b...

journal_title：Current opinion in structural biology

authors： Sharp KA,Honig B

更新日期：1995-06-01 00:00:00

abstract：:Covalent modifications of histone proteins regulate a wide variety of cellular processes. Methylation of histone H3K79 and H3K4 is associated with active transcription and is catalyzed by Dot1L and Set1, respectively. Both Dot1L and Set1 are activated by prior ubiquitination of histone H2B on K120 in a process termed ...

journal_title：Current opinion in structural biology

authors： Worden EJ,Wolberger C

更新日期：2019-12-01 00:00:00

abstract：:Transmembrane helix-helix interactions mediate the folding and assembly of membrane proteins. Recognition motifs range from GxxxG and leucine zippers to polar side chains and salt bridges. Some canonical membrane proteins contain local charge clusters that are important for folding and function, and which have to be c...

journal_title：Current opinion in structural biology

authors： Walther TH,Ulrich AS

更新日期：2014-08-01 00:00:00

abstract：:The past two years have seen the extension of our knowledge on the cellular prion protein structure with new NMR data on both the hamster and human proteins. In addition, the folding dynamics of two cellular prion proteins have been elucidated. There are now several examples of recombinant prion proteins that are able...

journal_title：Current opinion in structural biology

authors： Jackson GS,Clarke AR

更新日期：2000-02-01 00:00:00

abstract：:Given the increasing interest in protein-protein interactions, the prediction of these interactions from sequence and structural information has become a booming activity. CAPRI, the community-wide experiment for assessing blind predictions of protein-protein interactions, is playing an important role in fostering pro...

journal_title：Current opinion in structural biology

authors： Wodak SJ,Méndez R

更新日期：2004-04-01 00:00:00

abstract：:Molecular dynamics simulation studies of protein-nucleic acid complexes are more complicated than studies of either component alone-the force field has to be properly balanced, the systems tend to become very large, and a careful treatment of solvent and of electrostatic interactions is necessary. Recent investigation...

journal_title：Current opinion in structural biology

authors： Mackerell AD Jr,Nilsson L

更新日期：2008-04-01 00:00:00

abstract：:DNA photolyases are extremely efficient light-driven DNA repair enzymes that use the energy of a blue-light photon to 'inject' an electron onto UV-damaged DNA, catalyzing the splitting of mutagenic pyrimidine dimers. By contrast, cryptochromes use blue light to trigger signaling cascades in multicellular organisms, fu...

journal_title：Current opinion in structural biology

authors： Essen LO

更新日期：2006-02-01 00:00:00

abstract：:Current knowledge about the determinants of beta-sheet formation has been notably improved by the structural and kinetic analysis of model peptides, by mutagenesis experiments in proteins and by the statistical analysis of the protein structure database (Protein Data Bank; PDB). In the past year, several peptides comp...

journal_title：Current opinion in structural biology

authors： Lacroix E,Kortemme T,Lopez de la Paz M,Serrano L

更新日期：1999-08-01 00:00:00

abstract：:Proteins are chief actors in life that perform a myriad of exquisite functions. This diversity has been enabled through the evolution and diversification of protein folds. Analysis of sequences and structures strongly suggest that numerous protein pieces have been reused as building blocks and propagated to many moder...

journal_title：Current opinion in structural biology

authors： Romero-Romero S,Kordes S,Michel F,Höcker B

更新日期：2021-01-13 00:00:00

abstract：:Helical filaments were the first structures to be reconstructed in three dimensions from electron microscopic images, and continue to be extensively studied due to the large number of such helical polymers found in biology. In principle, a single image of a helical polymer provides all of the different projections nee...

journal_title：Current opinion in structural biology

authors： Egelman EH

更新日期：2007-10-01 00:00:00

abstract：:Membrane protein structural biology is enjoying a steady acceleration in the rate of success. Nevertheless, numerous membrane protein targets are resistant to the traditional approach of directly crystallizing detergent solubilized and purified protein and the 'niche market' of lipidic phase crystallization is emergin...

journal_title：Current opinion in structural biology

authors： Johansson LC,Wöhri AB,Katona G,Engström S,Neutze R

更新日期：2009-08-01 00:00:00

abstract：:Although intermediates have long been recognised as fascinating species that form during the folding of large proteins, the role that intermediates play in the folding of small, single-domain proteins has been widely debated. Recent discoveries using new, sensitive methods of detection and studies combining simulation...

journal_title：Current opinion in structural biology

authors： Brockwell DJ,Radford SE

更新日期：2007-02-01 00:00:00

abstract：:Congenital disorders of glycosylation (CDG) are a group of diseases that affect glycoprotein biogenesis. Eighteen different types of CDG have been defined genetically. They result from deficiencies in either the biosynthesis of oligosaccharide precursors or specific steps of N-glycan assembly, resulting in the absence...

journal_title：Current opinion in structural biology

authors： Freeze HH,Aebi M

更新日期：2005-10-01 00:00:00

abstract：:O-Glycosylation is an increasingly recognized modification of intracellular proteins in all kingdoms of life, and its occurrence in protists has been investigated to understand its evolution and its roles in the virulence of unicellular pathogens. We focus here on two kinds of glycoregulation found in unicellular euka...

journal_title：Current opinion in structural biology

authors： West CM,Kim HW

更新日期：2019-06-01 00:00:00

abstract：:Recently, advances have been made in methods and applications that integrate electron microscopy density maps and comparative modeling to produce atomic structures of macromolecular assemblies. Electron microscopy can benefit from comparative modeling through the fitting of comparative models into electron microscopy ...

journal_title：Current opinion in structural biology

authors： Topf M,Sali A

更新日期：2005-10-01 00:00:00

abstract：:If protein structure prediction methods are to make any impact on the impending onerous task of analyzing the large numbers of unknown protein sequences generated by the ongoing genome-sequencing projects, it is vital that they make the difficult transition from computational 'gedankenexperiments' to practical softwar...

journal_title：Current opinion in structural biology

authors： Jones DT

更新日期：1997-06-01 00:00:00

abstract：:Force spectroscopy has developed into an indispensable tool for studying folding and binding of proteins on a single molecule level in real time. Design of the pulling geometry allows tuning the reaction coordinate in a very precise manner. Many recent experiments have taken advantage of this possibility and have prov...

journal_title：Current opinion in structural biology

authors： Zoldák G,Rief M

更新日期：2013-02-01 00:00:00