Structural biology of telomerase and its interaction at telomeres.

abstract：:The radical intermediates generated during the catalytic cycles of adenosylcobalamin-dependent enzymes occur in pairs. The positions of radicals residing on the cofactor, substrate or protein, relative to the position of the low-spin Co(2+) from the cob(II)alamin intermediate, can be extracted from electron paramagnet...

journal_title：Current opinion in structural biology

authors： Reed GH,Mansoorabadi SO

更新日期：2003-12-01 00:00:00

abstract：:Multivalent molecules with a precise array of recognition elements that interact with specific cell types are important for characterizing the topology of molecules on a cell surface. Applications ranging from the control of cellular signaling to drug delivery and tissue imaging rely on these surface-mapping molecules...

journal_title：Current opinion in structural biology

authors： Lee Y,Sampson NS

更新日期：2006-08-01 00:00:00

abstract：:Dynamin is an important component of membrane recycling at the plasma membrane and, potentially, within the cell. The role of dynamin in clathrin-mediated endocytosis has been based on numerous endocytosis assays, as well as on the discovery and gross characterization of the assembled spiral structure of dynamin. Rece...

journal_title：Current opinion in structural biology

authors： Hinshaw JE

更新日期：1999-04-01 00:00:00

abstract：:Zinc finger proteins are among the most abundant proteins in eukaryotic genomes. Their functions are extraordinarily diverse and include DNA recognition, RNA packaging, transcriptional activation, regulation of apoptosis, protein folding and assembly, and lipid binding. Zinc finger structures are as diverse as their f...

journal_title：Current opinion in structural biology

authors： Laity JH,Lee BM,Wright PE

更新日期：2001-02-01 00:00:00

abstract：:Akt1-3 (Akt) are a subset of the AGC protein Ser/Thr kinase family and play important roles in cell growth, metabolic regulation, cancer, and other diseases. We describe some of the roles of Akt in cell signaling and the biochemical and structural mechanisms of the regulation of Akt catalysis by the phospholipid PIP3 ...

journal_title：Current opinion in structural biology

authors： Cole PA,Chu N,Salguero AL,Bae H

更新日期：2019-12-01 00:00:00

abstract：:The three-dimensional structures of several biotin-binding proteins are now known, giving insights into the molecular architecture of the binding sites for biotin. In combination with biochemical and computational approaches, these structural insights provide the basis for our present understanding of biotin-protein i...

journal_title：Current opinion in structural biology

authors： Lindqvist Y,Schneider G

更新日期：1996-12-01 00:00:00

abstract：:Covalent modifications of histone proteins regulate a wide variety of cellular processes. Methylation of histone H3K79 and H3K4 is associated with active transcription and is catalyzed by Dot1L and Set1, respectively. Both Dot1L and Set1 are activated by prior ubiquitination of histone H2B on K120 in a process termed ...

journal_title：Current opinion in structural biology

authors： Worden EJ,Wolberger C

更新日期：2019-12-01 00:00:00

abstract：:Magic-angle spinning solid-state NMR experiments are well suited to investigating the structures and mechanisms of important proteins that are inaccessible to X-ray crystallography and solution NMR spectroscopy, including membrane proteins and disease-related protein aggregates. Good progress has been made in the deve...

journal_title：Current opinion in structural biology

authors： Thompson LK

更新日期：2002-10-01 00:00:00

abstract：:The 5' m7GpppN cap plays an essential role in the life cycle of eukaryotic mRNA and is required for efficient pre-mRNA splicing, export, stability and translation. Nascent pre-mRNA is capped through a series of three enzymatic steps that result in a 5' N7-methyl guanosine linked by an inverted 5'-5' triphosphate bridg...

journal_title：Current opinion in structural biology

authors： Gu M,Lima CD

更新日期：2005-02-01 00:00:00

abstract：:Coiled coils have a rich history in the field of protein design and engineering. Novel structures, such as the first seven-helix coiled coil, continue to provide surprises and insights. Large-scale datasets quantifying the influence of systematic mutations on coiled-coil stability are a valuable new asset to the area....

journal_title：Current opinion in structural biology

authors： Grigoryan G,Keating AE

更新日期：2008-08-01 00:00:00

abstract：:Pyrrolopyrimidine containing natural products are widely distributed in Nature. The biosynthesis of the 7-deazapurine moiety that is common to all pyrrolopyrimidines entails multiple steps, one of which is a complex radical-mediated ring contraction reaction catalyzed by CDG synthase. Herein we review the biosynthetic...

journal_title：Current opinion in structural biology

authors： Bandarian V,Drennan CL

更新日期：2015-12-01 00:00:00

abstract：:The ClC proteins are members of a large family of chloride transport proteins, which are involved in a variety of physiological processes. All family members share a conserved molecular architecture consisting of a complex transmembrane transport domain and a soluble regulatory domain. To date, representative structur...

journal_title：Current opinion in structural biology

authors： Dutzler R

更新日期：2006-08-01 00:00:00

abstract：:The annotation of protein function at genomic scale is essential for day-to-day work in biology and for any systematic approach to the modeling of biological systems. Currently, functional annotation is essentially based on the expansion of the relatively small number of experimentally determined functions to large co...

journal_title：Current opinion in structural biology

authors： Valencia A

更新日期：2005-06-01 00:00:00

abstract：:Gamma-tubulin is as ubiquitous in eukaryotes as alpha- and beta-tubulin. Rather than forming part of the microtubule wall, however, gamma-tubulin is involved in microtubule nucleation. Although gamma-tubulin concentrates at microtubule-organizing centers, it also exists in a cytoplasmic complex whose size and complexi...

journal_title：Current opinion in structural biology

authors： Wiese C,Zheng Y

更新日期：1999-04-01 00:00:00

abstract：:A selection of World Wide Web sites relevant to papers published in this issue of Current Opinion in Structural Biology. ...

journal_title：Current opinion in structural biology

authors： Lakey JH,Reddy BV,Murray-Rust J

更新日期：2001-04-01 00:00:00

abstract：:Significant progress has been made over the past several years on structural studies of the eukaryotic translation initiation factors that facilitate the assembly of a translation-competent ribosome at the initiation codon of an mRNA. These structural studies have revealed the repeated use of a set of common structura...

journal_title：Current opinion in structural biology

authors： Sonenberg N,Dever TE

更新日期：2003-02-01 00:00:00

abstract：:New studies have shown that folding of beta-sheet proteins can occur with and without intermediates, with fast to slow refolding rates and late to very late transition states. These experiments demonstrate that, despite early speculation to the contrary, beta-sheet protein folding does not appear to be fundamentally d...

journal_title：Current opinion in structural biology

authors： Capaldi AP,Radford SE

更新日期：1998-02-01 00:00:00

abstract：:Biology has, and continues to be, shaped by evolutionary mechanisms. Within the past decade, local fitness landscapes have become experimentally tractable and are providing new perspectives on evolutionary mechanisms. Powered by next-generation sequencing, the impacts of all individual amino acid substitutions on func...

journal_title：Current opinion in structural biology

authors： Canale AS,Cote-Hammarlof PA,Flynn JM,Bolon DN

更新日期：2018-02-01 00:00:00

abstract：:Although intermediates have long been recognised as fascinating species that form during the folding of large proteins, the role that intermediates play in the folding of small, single-domain proteins has been widely debated. Recent discoveries using new, sensitive methods of detection and studies combining simulation...

journal_title：Current opinion in structural biology

authors： Brockwell DJ,Radford SE

更新日期：2007-02-01 00:00:00

abstract：:Protein aggregation constitutes a constant threat for the living cell as is demonstrated by the several pathologies in which the mechanisms to prevent it fail. It is therefore a question of increasing importance to understand in detail the defence strategies. Here we discuss how molecular interactions can represent a ...

journal_title：Current opinion in structural biology

authors： Pastore A,Temussi PA

更新日期：2012-02-01 00:00:00

abstract：:For various reasons, it seems sensible to redesign or design proteins from the inside out. Past approaches in this field have involved iterations of mutagenesis and characterisation to 'evolve' designs. Increasingly, combinatorial approaches are being taken to select 'fit' sequences from libraries of variant proteins....

journal_title：Current opinion in structural biology

authors： Woolfson DN

更新日期：2001-08-01 00:00:00

abstract：:Over recent years, nuclear magnetic resonance (NMR) spectroscopy has developed into a powerful mechanistic tool for the investigation of G protein-coupled receptors (GPCRs). NMR provides insights which underpin the dynamic nature of these important receptors and reveals experimental evidence for a complex conformation...

journal_title：Current opinion in structural biology

authors： Bostock MJ,Solt AS,Nietlispach D

更新日期：2019-08-01 00:00:00

abstract：:Small-angle X-ray scattering (SAXS) has emerged as an enabling integrative technique for comprehensive analyses of macromolecular structures and interactions in solution. Over the past two decades, SAXS has become a mainstay of the structural biologist's toolbox, supplying multiplexed measurements of molecular shape a...

journal_title：Current opinion in structural biology

authors： Brosey CA,Tainer JA

更新日期：2019-10-01 00:00:00

abstract：:The alpha helices of transmembrane proteins interact to form higher order structures. These interactions are frequently mediated by packing motifs (such as GxxxG) and polar residues. Recent structural data have revealed that small sidechains are able to both stabilize helical membrane proteins and allow conformational...

journal_title：Current opinion in structural biology

authors： Curran AR,Engelman DM

更新日期：2003-08-01 00:00:00

abstract：:For many protein families, such as serine proteinases or serine proteinase inhibitors, the family assignment predicts reactivity only in general terms. Both detailed specificity and quantitative reactivity are lacking. We believe that, for many such protein families, algorithms can be devised by defining the subset of...

journal_title：Current opinion in structural biology

authors： Laskowski M Jr,Qasim MA,Yi Z

更新日期：2003-02-01 00:00:00

abstract：:Helical filaments were the first structures to be reconstructed in three dimensions from electron microscopic images, and continue to be extensively studied due to the large number of such helical polymers found in biology. In principle, a single image of a helical polymer provides all of the different projections nee...

journal_title：Current opinion in structural biology

authors： Egelman EH

更新日期：2007-10-01 00:00:00

abstract：:Molecular dynamics simulation studies of protein-nucleic acid complexes are more complicated than studies of either component alone-the force field has to be properly balanced, the systems tend to become very large, and a careful treatment of solvent and of electrostatic interactions is necessary. Recent investigation...

journal_title：Current opinion in structural biology

authors： Mackerell AD Jr,Nilsson L

更新日期：2008-04-01 00:00:00

abstract：:Computational methods play an important role at all stages of the process of determining protein-protein interactions. They are used to predict potential interactions, to validate the results of high-throughput interaction screens and to analyze the protein networks inferred from interaction databases. ...

journal_title：Current opinion in structural biology

authors： Salwinski L,Eisenberg D

更新日期：2003-06-01 00:00:00

abstract：:Intrinsically Disordered Proteins (IDPs) perform a broad range of biological functions. Their relevance has motivated intense research activity seeking to characterize their sequence/structure/function relationships. However, the conformational plasticity of these molecules hampers the application of traditional struc...

journal_title：Current opinion in structural biology

authors： Cordeiro TN,Herranz-Trillo F,Urbanek A,Estaña A,Cortés J,Sibille N,Bernadó P

更新日期：2017-02-01 00:00:00

abstract：:Molecular flexibility over a wide time range is of central importance to the function of many proteins, both soluble and membrane. Revealing the modes of flexibility, their amplitudes, and time scales under physiological conditions is the challenge for spectroscopic methods, one of which is site-directed spin labeling...

journal_title：Current opinion in structural biology

authors： Hubbell WL,López CJ,Altenbach C,Yang Z

更新日期：2013-10-01 00:00:00