Gamma-tubulin complexes and their interaction with microtubule-organizing centers.

abstract：:The protein folding problem can be viewed as three different problems: defining the thermodynamic folding code; devising a good computational structure prediction algorithm; and answering Levinthal's question regarding the kinetic mechanism of how proteins can fold so quickly. Once regarded as a grand challenge, prote...

journal_title：Current opinion in structural biology

authors： Dill KA,Ozkan SB,Weikl TR,Chodera JD,Voelz VA

更新日期：2007-06-01 00:00:00

abstract：:Protein-protein interactions involving the catalytic domain of protein kinases are likely to be generally important in the regulation of signal transduction pathways, but are rather sparsely represented in crystal structures. Recently determined structures of the kinase domains of the mitogen-activated protein kinase ...

journal_title：Current opinion in structural biology

authors： Pellicena P,Kuriyan J

更新日期：2006-12-01 00:00:00

abstract：:The past decade has seen a revolution in our ability to engineer designer enzymes using genetic tools that mimic evolution on a laboratory timescale. Many excellent examples of directed evolution applied to a wide range of enzymes have clearly demonstrated its future role in adapting enzymes for use in the chemical in...

journal_title：Current opinion in structural biology

authors： Dalby PA

更新日期：2003-08-01 00:00:00

abstract：:Over 5000 halogenated natural products have been reported so far, many of these arising from the marine environment. The introduction of a halogen into a molecule can significantly impact its bioavailability and bioactivity. More recently enzymatic halogenation has been used to enable late stage functionalisation thro...

journal_title：Current opinion in structural biology

authors： Ludewig H,Molyneux S,Ferrinho S,Guo K,Lynch R,Gkotsi DS,Goss RJ

更新日期：2020-12-01 00:00:00

abstract：:The last two years have seen major advances in understanding the structural basis of bacterial cell envelope glycoconjugate biosynthesis, including capsules, lipopolysaccharide, teichoic acid, cellulose, and peptidoglycan. The recent crystal and cryo-electron microscopy structures of proteins involved in the initial g...

journal_title：Current opinion in structural biology

authors： Liston SD,Willis LM

更新日期：2021-01-08 00:00:00

abstract：:The folding and assembly of myosin motor proteins is essential for most movement processes at the cellular, but also at the organism level. Importantly, myosins, which represent a very diverse family of proteins, require the activity of general and specialized folding factors to develop their full motor function. The ...

journal_title：Current opinion in structural biology

authors： Hellerschmied D,Clausen T

更新日期：2014-04-01 00:00:00

abstract：:Kinetochores are large macromolecular assemblies that link chromosomes to spindle microtubules (MTs) during mitosis. Here we review recent advances in the study of core MT-binding kinetochore complexes using electron microcopy methods in vitro and nanometer-accuracy fluorescence microscopy in vivo. We synthesize these...

journal_title：Current opinion in structural biology

authors： Alushin G,Nogales E

更新日期：2011-10-01 00:00:00

abstract：:Centrioles are microtubule-based structures in eukaryotic cells. From organizing the microtubule cytoskeleton during interphase to focusing the mitotic spindle during mitosis, centrioles are busy at all stages of the cell cycle. One crucial interphase function of centrioles is to assemble cilia, microtubular projectio...

journal_title：Current opinion in structural biology

authors： Kumar D,Reiter J

更新日期：2020-11-04 00:00:00

abstract：:Intermediate filaments are built from one to several members of a multigene family encoding fibrous proteins that share a highly conserved hierarchic assembly plan for the formation of multistranded filaments from distinctly structured extended coiled coils. Despite the rather low primary sequence identity, intermedia...

journal_title：Current opinion in structural biology

authors： Herrmann H,Aebi U

更新日期：1998-04-01 00:00:00

abstract：:The three-dimensional structures of membrane proteins are essential for understanding their functions, interactions and architectures. Their requirement for lipids has hampered structure determination by conventional approaches. With optimized samples, it is possible to apply solution NMR methods to small membrane pro...

journal_title：Current opinion in structural biology

authors： Marassi FM,Opella SJ

更新日期：1998-10-01 00:00:00

abstract：:Protein biogenesis integrates multiple finely regulated mechanisms, ensuring nascent polypeptide chains are correctly enzymatically processed, targeted to membranes and folded to native structure. Recent studies show that the cellular translation machinery serves as hub that coordinates the maturation events in space ...

journal_title：Current opinion in structural biology

authors： Gloge F,Becker AH,Kramer G,Bukau B

更新日期：2014-02-01 00:00:00

abstract：:Progress in structural biology has begun to reveal the precise architecture of integral membrane proteins. However, the manner in which these complex structures are achieved remains unclear. Recent developments are starting to shed light on the unfolding and folding of a small but growing number of membrane proteins. ...

journal_title：Current opinion in structural biology

authors： Booth PJ,Curnow P

更新日期：2006-08-01 00:00:00

abstract：:ATP-binding cassette (ABC)-type ATPases are chemo-mechanical engines for diverse biological pathways. ABC ATPase domains act not only in ABC transporters but also in DNA mismatch, nucleotide excision and double-strand break repair enzymes, as well as in chromosome segregation. Atomic-resolution crystal structures sugg...

journal_title：Current opinion in structural biology

authors： Hopfner KP,Tainer JA

更新日期：2003-04-01 00:00:00

abstract：:Recent studies from several laboratories have provided evidence that cell surface complex carbohydrates play key roles in the regulation of developmentally relevant signal transduction events. The demonstration that Fringe, a known modifier of Notch function, is a fucose-specific N-acetylglucosaminyltransferase provid...

journal_title：Current opinion in structural biology

authors： Haltiwanger RS

更新日期：2002-10-01 00:00:00

abstract：:Rapid advances in the cloning and expression of glycosyltransferase genes, especially from bacteria, could open the way to overcoming difficulties in the mass production of oligosaccharides. The large-scale production of oligosaccharides using either glycosyltransferases isolated from engineered microorganisms or whol...

journal_title：Current opinion in structural biology

authors： Endo T,Koizumi S

更新日期：2000-10-01 00:00:00

abstract：:Malaria remains the world's most prevalent human parasitic disease. Because of the rapid spread of drug resistance in parasites, there is an urgent need to identify diverse new drug targets. One group of proteases that are emerging as targets for novel antimalarials are the metalloaminopeptidases. These enzymes cataly...

journal_title：Current opinion in structural biology

authors： McGowan S

更新日期：2013-12-01 00:00:00

abstract：:Force can drive conformational changes in proteins, as well as modulate their stability and the affinity of their complexes, allowing a mechanical input to be converted into a biochemical output. These properties have been utilised by nature and force is now recognised to be widely used at the cellular level. The effe...

journal_title：Current opinion in structural biology

authors： Chen Y,Radford SE,Brockwell DJ

更新日期：2015-02-01 00:00:00

abstract：:The 26S proteasome is the essential compartmental protease in eukaryotic cells required for the ubiquitin-dependent clearance of damaged polypeptides and obsolete regulatory proteins. Recently, a combination of high-resolution structural, biochemical, and biophysical studies has provided crucial new insights into the ...

journal_title：Current opinion in structural biology

authors： Greene ER,Dong KC,Martin A

更新日期：2020-04-01 00:00:00

abstract：:Synapses are cell adhesion structures specialized for signal transmission between neurons. At the synapse, presynaptic and postsynaptic terminals of neurons are functionally connected but spatially separated and form a cleft. Membrane receptor-like cell adhesion molecules and secreted proteins in the synaptic cleft (s...

journal_title：Current opinion in structural biology

authors： Yamagata A,Fukai S

更新日期：2019-02-01 00:00:00

abstract：:ATP synthases are rotary enzymes found in bacteria, chloroplasts, and mitochondria. These complexes produce the majority of cellular ATP in aerobic cells using energy from the transmembrane proton motive force established by the electron transport chain. In mitochondria, dimeric ATP synthase is essential for formation...

journal_title：Current opinion in structural biology

authors： Guo H,Rubinstein JL

更新日期：2018-10-01 00:00:00

abstract：:Recent advances in algorithmic techniques and statistics of sequence alignment have helped to make near-optimal alignments an important tool in the delineation of biologically valid alignments. Near-optimal alignments serve to judge the reliability of portions of a sequence alignment. The statistical assessment of sub...

journal_title：Current opinion in structural biology

authors： Vingron M

更新日期：1996-06-01 00:00:00

abstract：:Although intermediates have long been recognised as fascinating species that form during the folding of large proteins, the role that intermediates play in the folding of small, single-domain proteins has been widely debated. Recent discoveries using new, sensitive methods of detection and studies combining simulation...

journal_title：Current opinion in structural biology

authors： Brockwell DJ,Radford SE

更新日期：2007-02-01 00:00:00

abstract：:Recent developments in implicit solvent models may be compared in terms of accuracy and computational efficiency. Based on improvements in the accuracy of generalized Born methods and the speed of Poisson-Boltzmann solvers, it appears that the two techniques are converging to a point at which both will be suitable for...

journal_title：Current opinion in structural biology

authors： Baker NA

更新日期：2005-04-01 00:00:00

abstract：:Recent advances in atomic force microscopy (AFM) imaging of nucleic acids include the visualization of DNA and RNA incorporated into devices and patterns, and into structures based on their sequences or sequence recognition. AFM imaging of nuclear structures has contributed to advances in telomere research and to our ...

journal_title：Current opinion in structural biology

authors： Hansma HG,Kasuya K,Oroudjev E

更新日期：2004-06-01 00:00:00

abstract：:Molecular flexibility over a wide time range is of central importance to the function of many proteins, both soluble and membrane. Revealing the modes of flexibility, their amplitudes, and time scales under physiological conditions is the challenge for spectroscopic methods, one of which is site-directed spin labeling...

journal_title：Current opinion in structural biology

authors： Hubbell WL,López CJ,Altenbach C,Yang Z

更新日期：2013-10-01 00:00:00

abstract：:This review focuses on pharmacophore approaches in researching protein interfaces that bind protein ligands. Pharmacophore descriptions of binding interfaces that employ molecular dynamics simulation can account for effects of solvation and conformational flexibility. In addition, these calculations provide an approxi...

journal_title：Current opinion in structural biology

authors： Shrestha R,Fajardo JE,Fiser A

更新日期：2021-01-21 00:00:00

abstract：:Cells have evolved intricate ways to propagate signals through signaling networks rich in crosstalks between signaling pathways and feedforward and feedback loops. The enzymatic products of phosphorylation-dependent signaling, namely phosphopeptides, can be identified and quantified systematically by mass spectrometry...

journal_title：Current opinion in structural biology

authors： Gingras AC,Wong CJ

更新日期：2016-12-01 00:00:00

abstract：:Arf GTPases are major regulators of membrane traffic and organelle structure in eukaryotes where they recruit many different effectors, including components of vesicular coats, proteins that tether membranes, sort lipids or have diverse other functions in vesicular traffic, and bacterial proteins that divert Arf funct...

journal_title：Current opinion in structural biology

authors： Cherfils J

更新日期：2014-12-01 00:00:00

abstract：:This review emphasizes the effects of naturally occurring mutations on structural features and physico-chemical properties of proteins. The basic protein characteristics considered are stability, dynamics, and the binding of proteins and methods for assessing effects of mutations on these macromolecular characteristic...

journal_title：Current opinion in structural biology

authors： Kucukkal TG,Petukh M,Li L,Alexov E

更新日期：2015-06-01 00:00:00

abstract：:Force spectroscopy has developed into an indispensable tool for studying folding and binding of proteins on a single molecule level in real time. Design of the pulling geometry allows tuning the reaction coordinate in a very precise manner. Many recent experiments have taken advantage of this possibility and have prov...

journal_title：Current opinion in structural biology

authors： Zoldák G,Rief M

更新日期：2013-02-01 00:00:00