Antibody engineering.

abstract：:This review focuses on pharmacophore approaches in researching protein interfaces that bind protein ligands. Pharmacophore descriptions of binding interfaces that employ molecular dynamics simulation can account for effects of solvation and conformational flexibility. In addition, these calculations provide an approxi...

journal_title：Current opinion in structural biology

authors： Shrestha R,Fajardo JE,Fiser A

更新日期：2021-01-21 00:00:00

abstract：:Recent advances in atomic force microscopy (AFM) imaging of nucleic acids include the visualization of DNA and RNA incorporated into devices and patterns, and into structures based on their sequences or sequence recognition. AFM imaging of nuclear structures has contributed to advances in telomere research and to our ...

journal_title：Current opinion in structural biology

authors： Hansma HG,Kasuya K,Oroudjev E

更新日期：2004-06-01 00:00:00

abstract：:The structure of the visual pigment rhodopsin in the dark state was first investigated by electron microscopy (EM). More recently, rhodopsin has been crystallised in two different space groups--a tetragonal P4(1) crystal form and a trigonal P3(1) packing arrangement. The structures of the pigment, determined by X-ray ...

journal_title：Current opinion in structural biology

authors： Schertler GF

更新日期：2005-08-01 00:00:00

abstract：:Transition states in protein folding are being analyzed experimentally and by computer simulation to reveal the sequence of events in the folding and unfolding of proteins at high resolution. Results of this analysis have enabled the reconstruction of folding pathways in vitro and open the prospect of analyzing pathwa...

journal_title：Current opinion in structural biology

authors： Fersht AR

更新日期：1995-02-01 00:00:00

abstract：:Newly solved chaperone structures include the thermosome, a group II chaperonin, and a small heat-shock protein. Novel ideas on chaperone mechanism are presented in the forced unfolding hypothesis of GroEL action. Structures of chaperone-pilin complexes reveal the mechanism of chaperone interaction in bacterial pilus ...

journal_title：Current opinion in structural biology

authors： Saibil H

更新日期：2000-04-01 00:00:00

abstract：:Magic-angle-spinning (MAS) solid-state NMR spectroscopy is a versatile technique to elucidate functionally important protein-ligand interactions in lipid membranes. Here, we review recent solid-state NMR studies of membrane protein interactions with cholesterol, lipids, transported substrates, and peptide ligands. The...

journal_title：Current opinion in structural biology

authors： Elkins MR,Hong M

更新日期：2019-08-01 00:00:00

abstract：:There has been significant recent progress in the computational design of protein interactions including the creation of novel heterodimers, homodimers, nanohedra, fibril caps and a protein crystal. Essential to these successes has been the use of innovative strategies for finding binding modes that are achievable, th...

journal_title：Current opinion in structural biology

authors： Der BS,Kuhlman B

更新日期：2013-08-01 00:00:00

abstract：:The polyadenosine (poly(A)) tail found on the 3'-end of almost all eukaryotic mRNAs is important for mRNA stability and regulation of translation. mRNA 3'-end processing occurs co-transcriptionally and involves more than 20 proteins to specifically recognize the polyadenylation site, cleave the pre-mRNA, add a poly(A)...

journal_title：Current opinion in structural biology

authors： Kumar A,Clerici M,Muckenfuss LM,Passmore LA,Jinek M

更新日期：2019-12-01 00:00:00

abstract：:Hydrogen-deuterium exchange (HDX) mass spectrometry (MS) can provide information about proteins that can be challenging to obtain by other means. Structure/function relationships, binding interactions, and the effects of modification have all been measured with HDX MS for a diverse and growing array of signaling prote...

journal_title：Current opinion in structural biology

authors： Harrison RA,Engen JR

更新日期：2016-12-01 00:00:00

abstract：:The winged helix proteins constitute a subfamily within the large ensemble of helix-turn-helix proteins. Since the discovery of the winged helix/fork head motif in 1993, a large number of topologically related proteins with diverse biological functions have been characterized by X-ray crystallography and solution NMR ...

journal_title：Current opinion in structural biology

authors： Gajiwala KS,Burley SK

更新日期：2000-02-01 00:00:00

abstract：:Histones are highly positively charged proteins that wrap our genome. Their surface properties also make them prone to nonspecific interactions and aggregation. A class of proteins known as histone chaperones is dedicated to safeguard histones by aiding their proper incorporation into nucleosomes. Histone chaperones f...

journal_title：Current opinion in structural biology

authors： Hondele M,Ladurner AG

更新日期：2011-12-01 00:00:00

abstract：:Integrative structural modeling uses multiple types of input information and proceeds in four stages: (i) gathering information, (ii) designing model representation and converting information into a scoring function, (iii) sampling good-scoring models, and (iv) analyzing models and information. In the first stage, unc...

journal_title：Current opinion in structural biology

authors： Schneidman-Duhovny D,Pellarin R,Sali A

更新日期：2014-10-01 00:00:00

abstract：:Bacterial transcription initiation is controlled by sigma factors, the RNA polymerase (RNAP) subunits responsive for promoter specificity. While the primary sigma factor ensures the bulk of transcription during growth, a major strategy used by bacteria to regulate gene expression consists of modifying the RNAP promote...

journal_title：Current opinion in structural biology

authors： Campagne S,Allain FH,Vorholt JA

更新日期：2015-02-01 00:00:00

abstract：:You want to know how proteins do it? Take a walk in protein fold space. More often than not you will get a clue if not the answer. If you know what you are looking for and how to find it. In fact, there is more information than we can presently handle. Charting fold space and chasing its creatures has occupied us for ...

journal_title：Current opinion in structural biology

authors： Sippl MJ

更新日期：2009-06-01 00:00:00

abstract：:ATP-dependent initiation factors help process replication origins and coordinate replisome assembly to control the onset of DNA synthesis. Although the specific properties and regulatory mechanisms of initiator proteins can vary greatly between different organisms, certain nucleotide-binding elements and assembly patt...

journal_title：Current opinion in structural biology

authors： Duderstadt KE,Berger JM

更新日期：2013-02-01 00:00:00

abstract：:C-type lectins are the largest and most diverse family of mammalian carbohydrate-binding proteins. They share a common protein fold, which provides the unifying basis for calcium-mediated carbohydrate recognition. Their involvement in a multitude of biological functions is remarkable. Here, we review the variety of ta...

journal_title：Current opinion in structural biology

authors： Keller BG,Rademacher C

更新日期：2020-06-01 00:00:00

abstract：:Optogenetic dimerizers are modular domains that can be utilized in a variety of versatile ways to modulate cellular biochemistry. Because of their modularity, many applications using these tools can be easily transferred to new targets without extensive engineering. While a number of photodimerizer systems are current...

journal_title：Current opinion in structural biology

authors： Spiltoir JI,Tucker CL

更新日期：2019-08-01 00:00:00

abstract：:Specialized metabolic enzymes and metabolite diversity evolve through a variety of mechanisms including promiscuity, changes in substrate specificity, modifications of gene expression and gene duplication. For example, gene duplication and substrate binding site changes led to the evolution of the glucosinolate biosyn...

journal_title：Current opinion in structural biology

authors： Leong BJ,Last RL

更新日期：2017-12-01 00:00:00

abstract：:Membrane protein structural biology is enjoying a steady acceleration in the rate of success. Nevertheless, numerous membrane protein targets are resistant to the traditional approach of directly crystallizing detergent solubilized and purified protein and the 'niche market' of lipidic phase crystallization is emergin...

journal_title：Current opinion in structural biology

authors： Johansson LC,Wöhri AB,Katona G,Engström S,Neutze R

更新日期：2009-08-01 00:00:00

abstract：:Complementary developments in comparative genomics, protein structure determination and in-depth comparison of protein sequences and structures have provided a better understanding of the prevailing trends in the emergence and diversification of protein domains. The investigation of deep relationships among different ...

journal_title：Current opinion in structural biology

authors： Aravind L,Mazumder R,Vasudevan S,Koonin EV

更新日期：2002-06-01 00:00:00

abstract：:Newly synthesized proteins leave the ribosome through a narrow tunnel in the large subunit. During ongoing synthesis, nascent protein chains are particularly sensitive to aggregation and degradation because they emerge from the ribosome in an unfolded state. In bacteria, the first protein to interact with nascent chai...

journal_title：Current opinion in structural biology

authors： Maier T,Ferbitz L,Deuerling E,Ban N

更新日期：2005-04-01 00:00:00

abstract：:Although intermediates have long been recognised as fascinating species that form during the folding of large proteins, the role that intermediates play in the folding of small, single-domain proteins has been widely debated. Recent discoveries using new, sensitive methods of detection and studies combining simulation...

journal_title：Current opinion in structural biology

authors： Brockwell DJ,Radford SE

更新日期：2007-02-01 00:00:00

abstract：:Magic-angle spinning solid-state NMR experiments are well suited to investigating the structures and mechanisms of important proteins that are inaccessible to X-ray crystallography and solution NMR spectroscopy, including membrane proteins and disease-related protein aggregates. Good progress has been made in the deve...

journal_title：Current opinion in structural biology

authors： Thompson LK

更新日期：2002-10-01 00:00:00

abstract：:Current knowledge about the determinants of beta-sheet formation has been notably improved by the structural and kinetic analysis of model peptides, by mutagenesis experiments in proteins and by the statistical analysis of the protein structure database (Protein Data Bank; PDB). In the past year, several peptides comp...

journal_title：Current opinion in structural biology

authors： Lacroix E,Kortemme T,Lopez de la Paz M,Serrano L

更新日期：1999-08-01 00:00:00

abstract：:Centrioles are microtubule-based structures in eukaryotic cells. From organizing the microtubule cytoskeleton during interphase to focusing the mitotic spindle during mitosis, centrioles are busy at all stages of the cell cycle. One crucial interphase function of centrioles is to assemble cilia, microtubular projectio...

journal_title：Current opinion in structural biology

authors： Kumar D,Reiter J

更新日期：2020-11-04 00:00:00

abstract：:The alpha helices of transmembrane proteins interact to form higher order structures. These interactions are frequently mediated by packing motifs (such as GxxxG) and polar residues. Recent structural data have revealed that small sidechains are able to both stabilize helical membrane proteins and allow conformational...

journal_title：Current opinion in structural biology

authors： Curran AR,Engelman DM

更新日期：2003-08-01 00:00:00

abstract：:2-Oxoacid:ferredoxin oxidoreductases (OFORs) are essential enzymes in microbial one-carbon metabolism. They use thiamine pyrophosphate to reversibly cleave carbon-carbon bonds, generating low potential (∼-500mV) electrons. Crystallographic analysis of a recently discovered OFOR, an oxalate oxidoreductase (OOR), has pr...

journal_title：Current opinion in structural biology

authors： Gibson MI,Chen PY,Drennan CL

更新日期：2016-12-01 00:00:00

abstract：:2D-IR spectroscopy has matured to a powerful technique to study the structure and dynamics of peptides, but its extension to larger proteins is still in its infancy, the major limitations being sensitivity and selectivity. Site-selective information requires measuring single vibrational probes at sub-millimolar concen...

journal_title：Current opinion in structural biology

authors： Koziol KL,Johnson PJ,Stucki-Buchli B,Waldauer SA,Hamm P

更新日期：2015-10-01 00:00:00

abstract：:Messenger RNAs often contain structures in their 5'-untranslated region that serve to regulate or promote expression of their gene product. Recent structural studies have revealed that riboswitches, which bind a variety of small molecule metabolites, including purine bases, S-adenosylmethionine, amino acids and cofact...

journal_title：Current opinion in structural biology

authors： Batey RT

更新日期：2006-06-01 00:00:00

abstract：:The annotation of protein function at genomic scale is essential for day-to-day work in biology and for any systematic approach to the modeling of biological systems. Currently, functional annotation is essentially based on the expansion of the relatively small number of experimentally determined functions to large co...

journal_title：Current opinion in structural biology

authors： Valencia A

更新日期：2005-06-01 00:00:00