Influence of core protein sequence on glycosaminoglycan assembly.

abstract：:Glycoproteins and protein-carbohydrate complexes in the worldwide Protein Data Bank (wwPDB) can be an excellent source of information for glycoscientists. Unfortunately, a rather large number of errors and inconsistencies is found in the glycan moieties of these 3D structures. This review illustrates frequent problems...

journal_title：Current opinion in structural biology

authors： Joosten RP,Lütteke T

更新日期：2017-06-01 00:00:00

abstract：:Aromatic polyketides are secondary metabolites that afford some of the most common antibiotics and anti-cancer drugs currently in clinical use. Not least because of their medical importance, the biosynthesis of these compounds has attracted considerable interest during the past few years; important advances have been ...

journal_title：Current opinion in structural biology

authors： Schneider G

更新日期：2005-12-01 00:00:00

abstract：:Cells are equipped with cosolvents that modulate protein folding and aggregation to withstand water stress. The effect of cosolvents on the aggregation rates depends on whether the polypeptide sequence is an intrinsically disordered protein (IDP) or can fold into a specific native structure. Cosolvents, which act as d...

journal_title：Current opinion in structural biology

authors： Reddy G,Muttathukattil AN,Mondal B

更新日期：2020-02-01 00:00:00

abstract：:Prediction and modeling of RNA structures has become an indispensable tool of biological research disciplines. Currently, reliable predictions require massive input of experimental data. Structure-forming elements are conventional base pairs, as well as a rapidly increasing repertoire of novel structural motifs. New d...

journal_title：Current opinion in structural biology

authors： Schuster P,Stadler PF,Renner A

更新日期：1997-04-01 00:00:00

abstract：:The biological functions of protein molecules are intimately dependent on their conformational dynamics. This aspect is particularly evident for disordered proteins, which constitute perhaps one-third of the human proteome. Therefore, structural ensembles often offer more useful representations of proteins than indivi...

journal_title：Current opinion in structural biology

authors： Bonomi M,Heller GT,Camilloni C,Vendruscolo M

更新日期：2017-02-01 00:00:00

abstract：:2-Oxoacid:ferredoxin oxidoreductases (OFORs) are essential enzymes in microbial one-carbon metabolism. They use thiamine pyrophosphate to reversibly cleave carbon-carbon bonds, generating low potential (∼-500mV) electrons. Crystallographic analysis of a recently discovered OFOR, an oxalate oxidoreductase (OOR), has pr...

journal_title：Current opinion in structural biology

authors： Gibson MI,Chen PY,Drennan CL

更新日期：2016-12-01 00:00:00

abstract：:This review focuses on recent structural insights into regulation and nucleic acid binding of Superfamily 2 (SF2)-type helicases as they relate to chromatin remodelers. We review structural features of the Chd1 chromatin remodeler regarding regulation of the ATPase motor, and discuss related strategies observed for ot...

journal_title：Current opinion in structural biology

authors： Hauk G,Bowman GD

更新日期：2011-12-01 00:00:00

abstract：:Magic-angle-spinning (MAS) solid-state NMR spectroscopy is a versatile technique to elucidate functionally important protein-ligand interactions in lipid membranes. Here, we review recent solid-state NMR studies of membrane protein interactions with cholesterol, lipids, transported substrates, and peptide ligands. The...

journal_title：Current opinion in structural biology

authors： Elkins MR,Hong M

更新日期：2019-08-01 00:00:00

abstract：:Recent advances in algorithmic techniques and statistics of sequence alignment have helped to make near-optimal alignments an important tool in the delineation of biologically valid alignments. Near-optimal alignments serve to judge the reliability of portions of a sequence alignment. The statistical assessment of sub...

journal_title：Current opinion in structural biology

authors： Vingron M

更新日期：1996-06-01 00:00:00

abstract：:The observed distribution of protein structures can give us important clues about the underlying evolutionary process, imposing important constraints on possible models. The availability of results from an increasing number of genome projects has made the development of these models an active area of research. Models ...

journal_title：Current opinion in structural biology

authors： Goldstein RA

更新日期：2008-04-01 00:00:00

abstract：:Complementary developments in comparative genomics, protein structure determination and in-depth comparison of protein sequences and structures have provided a better understanding of the prevailing trends in the emergence and diversification of protein domains. The investigation of deep relationships among different ...

journal_title：Current opinion in structural biology

authors： Aravind L,Mazumder R,Vasudevan S,Koonin EV

更新日期：2002-06-01 00:00:00

abstract：:Can a stereochemical recognition code explain sequence-specific protein-nucleic acid interactions? Whereas a code that is generally applicable to DNA-binding proteins of all known structural families is unattainable, the indications are that a code can describe at least some of the interactions of classical zinc finge...

journal_title：Current opinion in structural biology

authors： Choo Y,Klug A

更新日期：1997-02-01 00:00:00

abstract：:During the past few years, there have been exciting developments in the field of flavoenzymology. New flavoenzymes have been discovered that are implicated in a variety of biological processes, including cell signaling, chromatin remodeling and cell development. The structures of several of these new flavoenzymes have...

journal_title：Current opinion in structural biology

authors： De Colibus L,Mattevi A

更新日期：2006-12-01 00:00:00

abstract：:Peptide-mediated interactions are gaining increased attention due to their predominant roles in the many regulatory processes that involve dynamic interactions between proteins. The structures of such interactions provide an excellent starting point for their characterization and manipulation, and can provide leads fo...

journal_title：Current opinion in structural biology

authors： London N,Raveh B,Schueler-Furman O

更新日期：2013-12-01 00:00:00

abstract：:Demand for novel GPCR modulators is increasing as the association between the GPCR signaling pathway and numerous diseases such as cancers, psychological and metabolic disorders continues to be established. In silico structure-based drug design (SBDD) offers an outlet where researchers could exploit the accumulating s...

journal_title：Current opinion in structural biology

authors： Lee Y,Lazim R,Macalino SJY,Choi S

更新日期：2019-04-01 00:00:00

abstract：:ATP-binding cassette (ABC)-type ATPases are chemo-mechanical engines for diverse biological pathways. ABC ATPase domains act not only in ABC transporters but also in DNA mismatch, nucleotide excision and double-strand break repair enzymes, as well as in chromosome segregation. Atomic-resolution crystal structures sugg...

journal_title：Current opinion in structural biology

authors： Hopfner KP,Tainer JA

更新日期：2003-04-01 00:00:00

abstract：:This review focuses on pharmacophore approaches in researching protein interfaces that bind protein ligands. Pharmacophore descriptions of binding interfaces that employ molecular dynamics simulation can account for effects of solvation and conformational flexibility. In addition, these calculations provide an approxi...

journal_title：Current opinion in structural biology

authors： Shrestha R,Fajardo JE,Fiser A

更新日期：2021-01-21 00:00:00

abstract：:Electrostatics plays a major role in the stabilization and function of biomolecules; as such, it remains a major focus of theoretical and computational studies of macromolecules. Electrostatic interactions are long range, and strongly dependent on the solvent and ions surrounding the biomolecule under study. During th...

journal_title：Current opinion in structural biology

authors： Koehl P

更新日期：2006-04-01 00:00:00

abstract：:The structure of the visual pigment rhodopsin in the dark state was first investigated by electron microscopy (EM). More recently, rhodopsin has been crystallised in two different space groups--a tetragonal P4(1) crystal form and a trigonal P3(1) packing arrangement. The structures of the pigment, determined by X-ray ...

journal_title：Current opinion in structural biology

authors： Schertler GF

更新日期：2005-08-01 00:00:00

abstract：:O-Glycosylation is an increasingly recognized modification of intracellular proteins in all kingdoms of life, and its occurrence in protists has been investigated to understand its evolution and its roles in the virulence of unicellular pathogens. We focus here on two kinds of glycoregulation found in unicellular euka...

journal_title：Current opinion in structural biology

authors： West CM,Kim HW

更新日期：2019-06-01 00:00:00

abstract：:The ribosome undergoes numerous large-scale conformational changes during protein synthesis, but the molecular basis for these changes have been unclear. Recent cryo-electron microscopic and X-ray crystallographic structures of both the bacterial and eukaryotic ribosome now provide snapshots of the wide range of motio...

journal_title：Current opinion in structural biology

authors： Noeske J,Cate JH

更新日期：2012-12-01 00:00:00

abstract：:Since its debut in the mid 1970s, electron crystallography has been a valuable alternative in the structure determination of biological macromolecules. Its reliance on single-layered or double-layered two-dimensionally ordered arrays and the ability to obtain structural information from small and disordered crystals m...

journal_title：Current opinion in structural biology

authors： Pope CR,Unger VM

更新日期：2012-08-01 00:00:00

abstract：:Recently, major advances have been made toward increasing our understanding of small ribozyme structure and function. The first general acid-base catalytic mechanism for a ribozyme has been defined. Shifted nucleotide pK(a) values have been found to be surprisingly frequent structural elements. Finally, the dynamic na...

journal_title：Current opinion in structural biology

authors： Butcher SE

更新日期：2001-06-01 00:00:00

abstract：:You want to know how proteins do it? Take a walk in protein fold space. More often than not you will get a clue if not the answer. If you know what you are looking for and how to find it. In fact, there is more information than we can presently handle. Charting fold space and chasing its creatures has occupied us for ...

journal_title：Current opinion in structural biology

authors： Sippl MJ

更新日期：2009-06-01 00:00:00

abstract：:Biological macromolecules have evolved over billions of years to function inside cells, so it is not surprising that researchers studying the properties of such molecules, either in extracts or in purified form, take care to control factors that reflect the intracellular environment, such as pH, ionic strength and com...

journal_title：Current opinion in structural biology

authors： Ellis RJ

更新日期：2001-02-01 00:00:00

abstract：:O-GlcNAcylation is an enzymatic post-translational modification occurring in hundreds of protein substrates. This modification occurs through the addition of the monosaccharide N-acetylglucosamine to serine and threonine residues on intracellular proteins in the cytosol, nucleus, and mitochondria. As a highly dynamic ...

journal_title：Current opinion in structural biology

authors： Balana AT,Moon SP,Pratt MR

更新日期：2021-01-09 00:00:00

abstract：:CCA-adding enzymes (tRNA nucleotidyltransferases) are responsible for the maturation or repair of the functional 3' end of tRNAs. These enzymes are remarkable because they polymerize the essential nucleotides CCA onto the 3' terminus of tRNA precursors without using a nucleic acid template. Recent crystal structures, ...

journal_title：Current opinion in structural biology

authors： Xiong Y,Steitz TA

更新日期：2006-02-01 00:00:00

abstract：:Investigating the in vitro refolding of proteins that naturally reside in biological membranes is a notoriously difficult task. Biophysical studies on model systems are beginning to provide a sound physical basis for membrane protein folding that should help to alleviate this problem. Highlights of these studies inclu...

journal_title：Current opinion in structural biology

authors： Booth PJ,Curran AR

更新日期：1999-02-01 00:00:00

abstract：:A network of dynamic protein interactions with their protein partners, substrates, and ligands is known to be crucial for biological function. Revealing molecular and structural-based mechanisms at atomic resolution and in real-time is fundamental for achieving a basic understanding of cellular processes. These techni...

journal_title：Current opinion in structural biology

authors： Grossman M,Sela-Passwell N,Sagi I

更新日期：2011-10-01 00:00:00

abstract：:Synapses are cell adhesion structures specialized for signal transmission between neurons. At the synapse, presynaptic and postsynaptic terminals of neurons are functionally connected but spatially separated and form a cleft. Membrane receptor-like cell adhesion molecules and secreted proteins in the synaptic cleft (s...

journal_title：Current opinion in structural biology

authors： Yamagata A,Fukai S

更新日期：2019-02-01 00:00:00