Structure and function of the small ribozymes.

abstract：:Specific, noncovalent binding of biomolecules can only be understood by considering structural, thermodynamic, and kinetic issues. The theoretical foundations for such analyses have been clarified in the past year. Computational techniques for both particle-based and continuum models continue to improve and to yield u...

journal_title：Current opinion in structural biology

authors： McCammon JA

更新日期：1998-04-01 00:00:00

abstract：:2-Oxoacid:ferredoxin oxidoreductases (OFORs) are essential enzymes in microbial one-carbon metabolism. They use thiamine pyrophosphate to reversibly cleave carbon-carbon bonds, generating low potential (∼-500mV) electrons. Crystallographic analysis of a recently discovered OFOR, an oxalate oxidoreductase (OOR), has pr...

journal_title：Current opinion in structural biology

authors： Gibson MI,Chen PY,Drennan CL

更新日期：2016-12-01 00:00:00

abstract：:Diverse glycans on proteins impact cell and organism physiology, along with drug activity. Since many protein-based biotherapeutics are glycosylated and these glycans have biological activity, there is a desire to engineer glycosylation for recombinant protein-based biotherapeutics. Engineered glycosylation can impact...

journal_title：Current opinion in structural biology

authors： Chiang AW,Li S,Spahn PN,Richelle A,Kuo CC,Samoudi M,Lewis NE

更新日期：2016-10-01 00:00:00

abstract：:Since user-friendly atomic force microscopes came onto the market a few years ago, scientists have explored the response of many proteins to applied force. This field has now matured beyond the phenomenological with exciting recent developments, particularly with regards to research into biological questions. For exam...

journal_title：Current opinion in structural biology

authors： Forman JR,Clarke J

更新日期：2007-02-01 00:00:00

abstract：:Recently, new methods for determining time-evolving structures using infrared analogs of NMR spectroscopy have been introduced that have outstanding potential in structural biology. Already, within the past two years, structures of dipeptides, tripeptides and pentapeptides have been determined on much faster timescale...

journal_title：Current opinion in structural biology

authors： Zanni MT,Hochstrasser RM

更新日期：2001-10-01 00:00:00

abstract：:Advances in the crystallization of biological macromolecules have come not only from the application of biochemical, molecular biological and immunological principles and techniques, but also from continued efforts to understand the crystallization process. Developments in crystallization methodologies, protocols, and...

journal_title：Current opinion in structural biology

authors： Gilliland GL,Ladner JE

更新日期：1996-10-01 00:00:00

abstract：:Biology can teach the physical world of electronics, computing, materials science and manufacturing how to assemble complex functional devices and systems that operate at the molecular level. Our present capability to fabricate simple molecular tools, devices, materials and machines is primitive compared with the soph...

journal_title：Current opinion in structural biology

authors： Lowe CR

更新日期：2000-08-01 00:00:00

abstract：:The polycomb repressive complex 2 (PRC2) is a conserved multiprotein, repressive chromatin complex essential for development and maintenance of eukaryotic cellular identity. PRC2 comprises a trimeric core of SUZ12, EED and EZH1/2, which together with RBBP4/7 is sufficient to catalyse mono-methylation, di-methylation a...

journal_title：Current opinion in structural biology

authors： Glancy E,Ciferri C,Bracken AP

更新日期：2020-11-21 00:00:00

abstract：:Histones are highly positively charged proteins that wrap our genome. Their surface properties also make them prone to nonspecific interactions and aggregation. A class of proteins known as histone chaperones is dedicated to safeguard histones by aiding their proper incorporation into nucleosomes. Histone chaperones f...

journal_title：Current opinion in structural biology

authors： Hondele M,Ladurner AG

更新日期：2011-12-01 00:00:00

abstract：:The first high-resolution structure of a P-type ATPase, that of the Ca(2+)-ATPase of skeletal muscle sarcoplasmic reticulum, was published in 2000. This structure has provided many clues to how the Ca(2+)-ATPase might work, but no complete answers. The Ca(2+)-ATPase structure reveals no clear pathway from the cytoplas...

journal_title：Current opinion in structural biology

authors： Lee AG

更新日期：2002-08-01 00:00:00

abstract：:The past decade has witnessed the development of numerous systems for the presentation of antigens on the surface of self-assembling macromolecules. Although the sites for insertion were initially chosen empirically, the determination of the three-dimensional structures of a number of carrier macromolecules has enable...

journal_title：Current opinion in structural biology

authors： Lomonossoff GP,Johnson JE

更新日期：1996-04-01 00:00:00

abstract：:Ring-shaped nucleic acid translocases and helicases catalyze the directed and processive movement of nucleic acid strands to support essential transactions such as replication, transcription, and chromosome partitioning. Assembled typically as hexamers, ring helicase/translocase systems use coordinated cycles of nucle...

journal_title：Current opinion in structural biology

authors： O'Shea VL,Berger JM

更新日期：2014-04-01 00:00:00

abstract：:The bacterial cell wall is a complex polymeric structure with essential roles in defence, survival and pathogenesis. Common to both Gram-positive and Gram-negative bacteria is the mesh-like peptidoglycan sacculus that surrounds the outer leaflet of the cytoplasmic membrane. Recent crystallographic studies of enzymes t...

journal_title：Current opinion in structural biology

authors： Caveney NA,Li FK,Strynadka NC

更新日期：2018-12-01 00:00:00

abstract：:The alpha helices of transmembrane proteins interact to form higher order structures. These interactions are frequently mediated by packing motifs (such as GxxxG) and polar residues. Recent structural data have revealed that small sidechains are able to both stabilize helical membrane proteins and allow conformational...

journal_title：Current opinion in structural biology

authors： Curran AR,Engelman DM

更新日期：2003-08-01 00:00:00

abstract：:The polyadenosine (poly(A)) tail found on the 3'-end of almost all eukaryotic mRNAs is important for mRNA stability and regulation of translation. mRNA 3'-end processing occurs co-transcriptionally and involves more than 20 proteins to specifically recognize the polyadenylation site, cleave the pre-mRNA, add a poly(A)...

journal_title：Current opinion in structural biology

authors： Kumar A,Clerici M,Muckenfuss LM,Passmore LA,Jinek M

更新日期：2019-12-01 00:00:00

abstract：:Ribosome recycling usually occurs after canonical termination triggered by a stop codon. Additionally, ribosomes that are stalled by aberrant mRNAs need to be recognized and subsequently recycled. In eukaryotes and archaea, the factors involved in canonical termination and ribosome rescue are structurally and function...

journal_title：Current opinion in structural biology

authors： Franckenberg S,Becker T,Beckmann R

更新日期：2012-12-01 00:00:00

abstract：:The protein folding problem can be viewed as three different problems: defining the thermodynamic folding code; devising a good computational structure prediction algorithm; and answering Levinthal's question regarding the kinetic mechanism of how proteins can fold so quickly. Once regarded as a grand challenge, prote...

journal_title：Current opinion in structural biology

authors： Dill KA,Ozkan SB,Weikl TR,Chodera JD,Voelz VA

更新日期：2007-06-01 00:00:00

abstract：:Almost two decades after their identification, the components of the mitotic checkpoint are finally revealing their structural secrets. The activation of Mad2, a central piece of the checkpoint protein machinery, is linked to the rare ability of this protein to adopt two distinct topologies. Current models of checkpoi...

journal_title：Current opinion in structural biology

authors： Mapelli M,Musacchio A

更新日期：2007-12-01 00:00:00

abstract：:A remarkable group of proteins challenge the notions that protein sequence determines a unique three-dimensional structure, and that membrane and soluble proteins are very distinct. The pore-forming toxins typically transform from soluble, monomeric proteins to oligomers that form transmembrane channels. Recent struct...

journal_title：Current opinion in structural biology

authors： Tilley SJ,Saibil HR

更新日期：2006-04-01 00:00:00

abstract：:Understanding the structural rules that govern specific, high-affinity binding characteristic of aptamer-protein interactions is important in view of the increasing use of aptamers across many applications. From the modest number of 16 aptamer-protein structures currently available, trends are emerging. The flexible p...

journal_title：Current opinion in structural biology

authors： Gelinas AD,Davies DR,Janjic N

更新日期：2016-02-01 00:00:00

abstract：:Caspases are a family of aspartate-specific cysteine proteases that regulate cellular homeostasis through the mediation of apoptosis and inflammation. Despite keen interest in caspases as therapeutic targets for cancer, inflammatory, and neurodegenerative diseases, no active-site directed small molecule has yet succee...

journal_title：Current opinion in structural biology

authors： Murray J,Renslo AR

更新日期：2013-12-01 00:00:00

abstract：:Solid-state NMR (ssNMR) offers insight into the formation of protein complexes and ligand binding for a large range of molecular sizes and binding affinities. Recent instrumental and methodological progress has enabled novel possibilities for using multi-dimensional ssNMR to study molecular 3D structures and interacti...

journal_title：Current opinion in structural biology

authors： Baldus M

更新日期：2006-10-01 00:00:00

abstract：:Eukaryotic transcriptional coactivators are multi-subunit complexes that both modify chromatin and recognize histone modifications. Until recently, structural information on these large complexes has been limited to isolated enzymatic domains or chromatin-binding motifs. This review summarizes recent structural studie...

journal_title：Current opinion in structural biology

authors： Samara NL,Wolberger C

更新日期：2011-12-01 00:00:00

abstract：:The radical intermediates generated during the catalytic cycles of adenosylcobalamin-dependent enzymes occur in pairs. The positions of radicals residing on the cofactor, substrate or protein, relative to the position of the low-spin Co(2+) from the cob(II)alamin intermediate, can be extracted from electron paramagnet...

journal_title：Current opinion in structural biology

authors： Reed GH,Mansoorabadi SO

更新日期：2003-12-01 00:00:00

abstract：:Among the crystal structures of lectins determined recently, three--snowdrop lectin, jacalin and amaranthin--represent new lectin families. Their polypeptide folds share remarkably similar features and consist exclusively of beta structure. Autonomously folded beta-sheet subdomains, inter-related by a pseudothreefold ...

journal_title：Current opinion in structural biology

authors： Wright CS

更新日期：1997-10-01 00:00:00

abstract：:Fucose is a common terminal modification on protein and lipid glycans. Fucose can also be directly linked to protein via an O-linkage to Serine or Threonine residues located within consensus sequences contained in Epidermal Growth Factor-like (EGF) repeats and Thrombospondin Type 1 Repeats (TSRs). In this context, fuc...

journal_title：Current opinion in structural biology

authors： Holdener BC,Haltiwanger RS

更新日期：2019-06-01 00:00:00

abstract：:Lipidic mesophases have been used to produce diffraction-quality crystals of several membrane proteins. The mechanism by which the method works is a mystery. The thrust is to continue to use it whilst deciphering the underlying mechanism and solving the second 'phase problem' in membrane protein crystallography. The m...

journal_title：Current opinion in structural biology

authors： Caffrey M

更新日期：2000-08-01 00:00:00

abstract：:Recent advances in neutron and X-ray sources and instrumentation, new and improved scattering techniques, and molecular biology techniques, which have permitted facile preparation of samples, have each led to new opportunities in using small-angle scattering to study the conformations and interactions of biological ma...

journal_title：Current opinion in structural biology

authors： Trewhella J

更新日期：1997-10-01 00:00:00

abstract：:The biological functions of protein molecules are intimately dependent on their conformational dynamics. This aspect is particularly evident for disordered proteins, which constitute perhaps one-third of the human proteome. Therefore, structural ensembles often offer more useful representations of proteins than indivi...

journal_title：Current opinion in structural biology

authors： Bonomi M,Heller GT,Camilloni C,Vendruscolo M

更新日期：2017-02-01 00:00:00

abstract：:There has been fantastic progress in solving GPCR crystal structures. However, the ability of X-ray crystallography to guide the drug discovery process for GPCR targets is limited by the availability of accurate tools to explore receptor-ligand interactions. Visual inspection and molecular mechanics approaches cannot ...

journal_title：Current opinion in structural biology

authors： Heifetz A,James T,Southey M,Morao I,Aldeghi M,Sarrat L,Fedorov DG,Bodkin MJ,Townsend-Nicholson A

更新日期：2019-04-01 00:00:00