Near-optimal sequence alignment.

abstract：:TolC and its many homologues comprise an alpha-helical transperiplasmic tunnel embedded in the bacterial outer membrane by a contiguous beta-barrel channel, providing a large exit duct for diverse substrates. The 'channel-tunnel' is closed at its periplasmic entrance, but can be opened by an 'iris-like' mechanism when...

journal_title：Current opinion in structural biology

authors： Koronakis V,Andersen C,Hughes C

更新日期：2001-08-01 00:00:00

abstract：:Structure-specific DNA nucleases play important roles in various DNA transactions such as DNA replication, repair and recombination. These enzymes recognize loops and branched DNA structures. Recent structural studies have provided detailed insights into the functions of these enzymes. Structures of Holliday junction ...

journal_title：Current opinion in structural biology

authors： Nishino T,Ishino Y,Morikawa K

更新日期：2006-02-01 00:00:00

abstract：:Newly synthesized proteins leave the ribosome through a narrow tunnel in the large subunit. During ongoing synthesis, nascent protein chains are particularly sensitive to aggregation and degradation because they emerge from the ribosome in an unfolded state. In bacteria, the first protein to interact with nascent chai...

journal_title：Current opinion in structural biology

authors： Maier T,Ferbitz L,Deuerling E,Ban N

更新日期：2005-04-01 00:00:00

abstract：:The computational evaluation of protein-protein interactions will play an important role in organising the wealth of data being generated by high-throughput initiatives. Here we discuss future applications, report recent developments and identify areas requiring further investigation. Many functions have been develope...

journal_title：Current opinion in structural biology

authors： Moal IH,Moretti R,Baker D,Fernández-Recio J

更新日期：2013-12-01 00:00:00

abstract：:Advances in the crystallization of biological macromolecules have come not only from the application of biochemical, molecular biological and immunological principles and techniques, but also from continued efforts to understand the crystallization process. Developments in crystallization methodologies, protocols, and...

journal_title：Current opinion in structural biology

authors： Gilliland GL,Ladner JE

更新日期：1996-10-01 00:00:00

abstract：:Structures of three different pleckstrin homology domains have been determined within the past year. They have a common core consisting of a seven-stranded and strongly bent beta-sheet and a C-terminal alpha-helix that packs against the beta-sheet. Phosphatidylinositol 4,5-bisphosphate and related compounds specifical...

journal_title：Current opinion in structural biology

authors： Saraste M,Hyvönen M

更新日期：1995-06-01 00:00:00

abstract：:ATP synthases are rotary enzymes found in bacteria, chloroplasts, and mitochondria. These complexes produce the majority of cellular ATP in aerobic cells using energy from the transmembrane proton motive force established by the electron transport chain. In mitochondria, dimeric ATP synthase is essential for formation...

journal_title：Current opinion in structural biology

authors： Guo H,Rubinstein JL

更新日期：2018-10-01 00:00:00

abstract：:Recently, advances have been made in methods and applications that integrate electron microscopy density maps and comparative modeling to produce atomic structures of macromolecular assemblies. Electron microscopy can benefit from comparative modeling through the fitting of comparative models into electron microscopy ...

journal_title：Current opinion in structural biology

authors： Topf M,Sali A

更新日期：2005-10-01 00:00:00

abstract：:Toll-like receptors (TLRs) activate the innate immune system in response to invading pathogens. Although nucleic acids are one of the principal TLR ligands, they are not inherently pathogen-specific and, thus, carry the risk of triggering autoimmunity. There are multiple unique regulatory mechanisms aimed at preventin...

journal_title：Current opinion in structural biology

authors： Shimizu T

更新日期：2017-12-01 00:00:00

abstract：:Computational protein design - the search for amino acids that adopt defined structures and functions - has achieved significant successes in recent years. Design is often approached from the perspective of targeting the design objective one structure-sequence pair at a time. Here we summarize studies that take an alt...

journal_title：Current opinion in structural biology

authors： Friedland GD,Kortemme T

更新日期：2010-06-01 00:00:00

abstract：:Assembly line polyketide synthases (PKSs) are remarkable biosynthetic machines with considerable potential for structure-based engineering. Several types of protein-protein interactions, both within and between PKS modules, play important roles in the catalytic cycle of a multimodular PKS. Additionally, vectorial bios...

journal_title：Current opinion in structural biology

authors： Robbins T,Liu YC,Cane DE,Khosla C

更新日期：2016-12-01 00:00:00

abstract：:Structures of two restriction endonucleases, BamHI and PvuII, were reported in the past year. This doubles the number of restriction endonuclease structures now known from two to four, and enables a comparative analysis of their structures and modes of DNA recognition. Despite a lack of sequence homology between the e...

journal_title：Current opinion in structural biology

authors： Aggarwal AK

更新日期：1995-02-01 00:00:00

abstract：:The wide occurrence of sialic acids (Sia) in various chemical forms linked as monomers or polymers in an outstanding position in a multitude of complex carbohydrates of animals and microorganisms renders them as most versatile function modulators in cell biology and pathology. A survey is presented of recent advances ...

journal_title：Current opinion in structural biology

authors： Schauer R

更新日期：2009-10-01 00:00:00

abstract：:Recent developments in implicit solvent models may be compared in terms of accuracy and computational efficiency. Based on improvements in the accuracy of generalized Born methods and the speed of Poisson-Boltzmann solvers, it appears that the two techniques are converging to a point at which both will be suitable for...

journal_title：Current opinion in structural biology

authors： Baker NA

更新日期：2005-04-01 00:00:00

abstract：:Recently solved structures and proposed models have helped to reveal the structural characteristics of the beta-propeller fold, as well as the features that contribute to its high rigidity and stability. Possible strategies for identifying beta-propeller proteins in newly characterised sequences are helping to overcom...

journal_title：Current opinion in structural biology

authors： Fülöp V,Jones DT

更新日期：1999-12-01 00:00:00

abstract：:Congenital disorders of glycosylation (CDG) are a group of diseases that affect glycoprotein biogenesis. Eighteen different types of CDG have been defined genetically. They result from deficiencies in either the biosynthesis of oligosaccharide precursors or specific steps of N-glycan assembly, resulting in the absence...

journal_title：Current opinion in structural biology

authors： Freeze HH,Aebi M

更新日期：2005-10-01 00:00:00

abstract：:PUF proteins are RNA-binding proteins named for founding members PUMILIO and fem-3 binding factor (FBF). Together these proteins represent the range of known RNA recognition properties. PUMILIO is a prototypical PUF protein whose RNA sequence specificity is simple, elegant, and predictable. FBF displays differences in...

journal_title：Current opinion in structural biology

authors： Lu G,Dolgner SJ,Hall TM

更新日期：2009-02-01 00:00:00

abstract：:In vitro selection experiments are now routinely used to identify functional nucleic acid residues and structures, and have become a tool for studying molecular recognition, molecular biology, and molecular evolution. Technical innovations that have been made during the past year include the use of modified monomers t...

journal_title：Current opinion in structural biology

authors： Uphoff KW,Bell SD,Ellington AD

更新日期：1996-06-01 00:00:00

abstract：:You want to know how proteins do it? Take a walk in protein fold space. More often than not you will get a clue if not the answer. If you know what you are looking for and how to find it. In fact, there is more information than we can presently handle. Charting fold space and chasing its creatures has occupied us for ...

journal_title：Current opinion in structural biology

authors： Sippl MJ

更新日期：2009-06-01 00:00:00

abstract：:Computational methods play an important role at all stages of the process of determining protein-protein interactions. They are used to predict potential interactions, to validate the results of high-throughput interaction screens and to analyze the protein networks inferred from interaction databases. ...

journal_title：Current opinion in structural biology

authors： Salwinski L,Eisenberg D

更新日期：2003-06-01 00:00:00

abstract：:As the fundamental packing units of DNA in eukaryotes, nucleosomes play a central role in governing DNA accessibility in a variety of cellular processes. Our understanding of the mechanisms underlying this complex regulation has been aided by unique structural and dynamic perspectives offered by single molecule techni...

journal_title：Current opinion in structural biology

authors： Killian JL,Li M,Sheinin MY,Wang MD

更新日期：2012-02-01 00:00:00

abstract：:Few biological macromolecular complexes exhibit the combination of massive size and hierarchical, symmetrical architecture embodied in icosahedral viruses. X-ray crystallography, electron cryomicroscopy and small-angle X-ray scattering provide complementary approaches to studying these remarkable structures. Through a...

journal_title：Current opinion in structural biology

authors： Lee KK,Johnson JE

更新日期：2003-10-01 00:00:00

abstract：:Historically it has been virtually impossible to experimentally determine the contribution of residual protein entropy to fundamental protein activities such as the binding of ligands. Recent progress has illuminated the possibility of employing NMR relaxation methods to quantitatively determine the role of changes in...

journal_title：Current opinion in structural biology

authors： Wand AJ

更新日期：2013-02-01 00:00:00

abstract：:The accelerated elucidation of three-dimensional structures of protein complexes, both natural and designed, is providing new examples of large supramolecular assemblies with intriguing shapes. Those with high symmetry - based on the geometries of the Platonic solids - are particularly notable as their innately closed...

journal_title：Current opinion in structural biology

authors： Cannon KA,Ochoa JM,Yeates TO

更新日期：2019-04-01 00:00:00

abstract：:Due to the contribution of drug-target binding kinetics to drug efficacy, there is a high level of interest in developing methods to predict drug-target binding kinetic parameters. During the review period, a wide range of enhanced sampling molecular dynamics simulation-based methods has been developed for computing d...

journal_title：Current opinion in structural biology

authors： Nunes-Alves A,Kokh DB,Wade RC

更新日期：2020-10-01 00:00:00

abstract：:Improved rational drug design methods are needed to lower the cost and increase the success rate of drug discovery and development. Alchemical binding free energy calculations, one potential tool for rational design, have progressed rapidly over the past decade, but still fall short of providing robust tools for pharm...

journal_title：Current opinion in structural biology

authors： Chodera JD,Mobley DL,Shirts MR,Dixon RW,Branson K,Pande VS

更新日期：2011-04-01 00:00:00

abstract：:The polyadenosine (poly(A)) tail found on the 3'-end of almost all eukaryotic mRNAs is important for mRNA stability and regulation of translation. mRNA 3'-end processing occurs co-transcriptionally and involves more than 20 proteins to specifically recognize the polyadenylation site, cleave the pre-mRNA, add a poly(A)...

journal_title：Current opinion in structural biology

authors： Kumar A,Clerici M,Muckenfuss LM,Passmore LA,Jinek M

更新日期：2019-12-01 00:00:00

abstract：:Integrative structural modeling uses multiple types of input information and proceeds in four stages: (i) gathering information, (ii) designing model representation and converting information into a scoring function, (iii) sampling good-scoring models, and (iv) analyzing models and information. In the first stage, unc...

journal_title：Current opinion in structural biology

authors： Schneidman-Duhovny D,Pellarin R,Sali A

更新日期：2014-10-01 00:00:00

abstract：:The past twelve months have seen a renewal of interest in the therapeutic potential of members of the tumour necrosis factor receptor family and their cytokine ligands. This biomedical interest has spawned a number of structural studies, which have significantly deepened our understanding of the molecular basis for th...

journal_title：Current opinion in structural biology

authors： Jones EY

更新日期：2000-12-01 00:00:00

abstract：:Since user-friendly atomic force microscopes came onto the market a few years ago, scientists have explored the response of many proteins to applied force. This field has now matured beyond the phenomenological with exciting recent developments, particularly with regards to research into biological questions. For exam...

journal_title：Current opinion in structural biology

authors： Forman JR,Clarke J

更新日期：2007-02-01 00:00:00