Crystallization and structure determination of RNA.

abstract：:Protein engineering strategies for increasing stability can be improved by replacing random mutagenesis and high-throughput screening by approaches that include bioinformatics and computational design. Mutations can be focused on regions in the structure that are most flexible and involved in the early steps of therma...

journal_title：Current opinion in structural biology

authors： Wijma HJ,Floor RJ,Janssen DB

更新日期：2013-08-01 00:00:00

abstract：:The sema domain was first defined from sequence by Kolodkin and colleagues in the early 1990s, and constitutes the distinctive structural and functional element of semaphorins, their plexin receptors and the receptor tyrosine kinases MET and RON, three protein families with major roles in development, tissue regenerat...

journal_title：Current opinion in structural biology

authors： Gherardi E,Love CA,Esnouf RM,Jones EY

更新日期：2004-12-01 00:00:00

abstract：:Improved rational drug design methods are needed to lower the cost and increase the success rate of drug discovery and development. Alchemical binding free energy calculations, one potential tool for rational design, have progressed rapidly over the past decade, but still fall short of providing robust tools for pharm...

journal_title：Current opinion in structural biology

authors： Chodera JD,Mobley DL,Shirts MR,Dixon RW,Branson K,Pande VS

更新日期：2011-04-01 00:00:00

abstract：:Structure-specific DNA nucleases play important roles in various DNA transactions such as DNA replication, repair and recombination. These enzymes recognize loops and branched DNA structures. Recent structural studies have provided detailed insights into the functions of these enzymes. Structures of Holliday junction ...

journal_title：Current opinion in structural biology

authors： Nishino T,Ishino Y,Morikawa K

更新日期：2006-02-01 00:00:00

abstract：:Since user-friendly atomic force microscopes came onto the market a few years ago, scientists have explored the response of many proteins to applied force. This field has now matured beyond the phenomenological with exciting recent developments, particularly with regards to research into biological questions. For exam...

journal_title：Current opinion in structural biology

authors： Forman JR,Clarke J

更新日期：2007-02-01 00:00:00

abstract：:Membrane protein structural biology is enjoying a steady acceleration in the rate of success. Nevertheless, numerous membrane protein targets are resistant to the traditional approach of directly crystallizing detergent solubilized and purified protein and the 'niche market' of lipidic phase crystallization is emergin...

journal_title：Current opinion in structural biology

authors： Johansson LC,Wöhri AB,Katona G,Engström S,Neutze R

更新日期：2009-08-01 00:00:00

abstract：:Almost two decades after their identification, the components of the mitotic checkpoint are finally revealing their structural secrets. The activation of Mad2, a central piece of the checkpoint protein machinery, is linked to the rare ability of this protein to adopt two distinct topologies. Current models of checkpoi...

journal_title：Current opinion in structural biology

authors： Mapelli M,Musacchio A

更新日期：2007-12-01 00:00:00

abstract：:Maintenance of telomeres by the enzyme telomerase is essential for genomic stability and cell viability in ciliates, vertebrates and yeast. The minimal components of telomerase required for catalytic activity are the telomerase reverse transcriptase (TERT) protein and the template-containing telomerase RNA (TER). Rece...

journal_title：Current opinion in structural biology

authors： Theimer CA,Feigon J

更新日期：2006-06-01 00:00:00

abstract：:Akt1-3 (Akt) are a subset of the AGC protein Ser/Thr kinase family and play important roles in cell growth, metabolic regulation, cancer, and other diseases. We describe some of the roles of Akt in cell signaling and the biochemical and structural mechanisms of the regulation of Akt catalysis by the phospholipid PIP3 ...

journal_title：Current opinion in structural biology

authors： Cole PA,Chu N,Salguero AL,Bae H

更新日期：2019-12-01 00:00:00

abstract：:Structures of two restriction endonucleases, BamHI and PvuII, were reported in the past year. This doubles the number of restriction endonuclease structures now known from two to four, and enables a comparative analysis of their structures and modes of DNA recognition. Despite a lack of sequence homology between the e...

journal_title：Current opinion in structural biology

authors： Aggarwal AK

更新日期：1995-02-01 00:00:00

abstract：:Peptide-mediated interactions are gaining increased attention due to their predominant roles in the many regulatory processes that involve dynamic interactions between proteins. The structures of such interactions provide an excellent starting point for their characterization and manipulation, and can provide leads fo...

journal_title：Current opinion in structural biology

authors： London N,Raveh B,Schueler-Furman O

更新日期：2013-12-01 00:00:00

abstract：:G-protein-coupled receptors (GPCRs) participate in virtually all physiological processes. They constitute the largest and most structurally conserved family of signaling molecules. Several class C GPCRs have been shown to exist as dimers in their active form and growing evidence indicates that many, if not all, class ...

journal_title：Current opinion in structural biology

authors： Fotiadis D,Jastrzebska B,Philippsen A,Müller DJ,Palczewski K,Engel A

更新日期：2006-04-01 00:00:00

abstract：:Protein design efforts are beginning to yield molecules with many of the properties of natural proteins. Such experiments are informed by and contribute to our understanding of the sequence determinants of protein folding and stability. The most important design elements seem to be the proper placement of hydrophobic ...

journal_title：Current opinion in structural biology

authors： Cordes MH,Davidson AR,Sauer RT

更新日期：1996-02-01 00:00:00

abstract：:Proteins are chief actors in life that perform a myriad of exquisite functions. This diversity has been enabled through the evolution and diversification of protein folds. Analysis of sequences and structures strongly suggest that numerous protein pieces have been reused as building blocks and propagated to many moder...

journal_title：Current opinion in structural biology

authors： Romero-Romero S,Kordes S,Michel F,Höcker B

更新日期：2021-01-13 00:00:00

abstract：:The folding and assembly of myosin motor proteins is essential for most movement processes at the cellular, but also at the organism level. Importantly, myosins, which represent a very diverse family of proteins, require the activity of general and specialized folding factors to develop their full motor function. The ...

journal_title：Current opinion in structural biology

authors： Hellerschmied D,Clausen T

更新日期：2014-04-01 00:00:00

abstract：:Cells are equipped with cosolvents that modulate protein folding and aggregation to withstand water stress. The effect of cosolvents on the aggregation rates depends on whether the polypeptide sequence is an intrinsically disordered protein (IDP) or can fold into a specific native structure. Cosolvents, which act as d...

journal_title：Current opinion in structural biology

authors： Reddy G,Muttathukattil AN,Mondal B

更新日期：2020-02-01 00:00:00

abstract：:Structures of three different pleckstrin homology domains have been determined within the past year. They have a common core consisting of a seven-stranded and strongly bent beta-sheet and a C-terminal alpha-helix that packs against the beta-sheet. Phosphatidylinositol 4,5-bisphosphate and related compounds specifical...

journal_title：Current opinion in structural biology

authors： Saraste M,Hyvönen M

更新日期：1995-06-01 00:00:00

abstract：:The encounter complex of two proteins is a dynamic intermediate state that guides proteins to their binding site, thus enhancing the rate of complex formation. It is particularly useful for complexes that must balance a biological requirement for high turnover with the need for specific binding, such as electron trans...

journal_title：Current opinion in structural biology

authors： Schilder J,Ubbink M

更新日期：2013-12-01 00:00:00

abstract：:During protein evolution, novel domain arrangements are continuously formed. Rearrangements are important for the creation of molecular biodiversity and for functional molecular changes which underlie developmental shifts in the bauplan of organisms. Here we review the mechanisms by which new arrangements arise and th...

journal_title：Current opinion in structural biology

authors： Bornberg-Bauer E,Albà MM

更新日期：2013-06-01 00:00:00

abstract：:The application of biocatalysis for the synthesis of glycans and glycoconjugates is a well-established and successful strategy, both for small and large scale synthesis. Compared to chemical synthesis, is has the advantage of high selectivity, but biocatalysis had been largely limited to natural glycans both in terms ...

journal_title：Current opinion in structural biology

authors： Pallister E,Gray CJ,Flitsch SL

更新日期：2020-12-01 00:00:00

abstract：:This review focuses on pharmacophore approaches in researching protein interfaces that bind protein ligands. Pharmacophore descriptions of binding interfaces that employ molecular dynamics simulation can account for effects of solvation and conformational flexibility. In addition, these calculations provide an approxi...

journal_title：Current opinion in structural biology

authors： Shrestha R,Fajardo JE,Fiser A

更新日期：2021-01-21 00:00:00

abstract：:The modulation of enzyme function is a key regulatory feature of biological systems. The ability to engineer synthetic enzymes that can be controlled by any arbitrary signal would enable a wide array of sensing applications and therapeutics and provide us with powerful tools for the basic study of biology. Here severa...

journal_title：Current opinion in structural biology

authors： Ostermeier M

更新日期：2009-08-01 00:00:00

abstract：:This review focuses on recent structural insights into regulation and nucleic acid binding of Superfamily 2 (SF2)-type helicases as they relate to chromatin remodelers. We review structural features of the Chd1 chromatin remodeler regarding regulation of the ATPase motor, and discuss related strategies observed for ot...

journal_title：Current opinion in structural biology

authors： Hauk G,Bowman GD

更新日期：2011-12-01 00:00:00

abstract：:Electrostatics plays a major role in the stabilization and function of biomolecules; as such, it remains a major focus of theoretical and computational studies of macromolecules. Electrostatic interactions are long range, and strongly dependent on the solvent and ions surrounding the biomolecule under study. During th...

journal_title：Current opinion in structural biology

authors： Koehl P

更新日期：2006-04-01 00:00:00

abstract：:The alpha helices of transmembrane proteins interact to form higher order structures. These interactions are frequently mediated by packing motifs (such as GxxxG) and polar residues. Recent structural data have revealed that small sidechains are able to both stabilize helical membrane proteins and allow conformational...

journal_title：Current opinion in structural biology

authors： Curran AR,Engelman DM

更新日期：2003-08-01 00:00:00

abstract：:Recently, major advances have been made toward increasing our understanding of small ribozyme structure and function. The first general acid-base catalytic mechanism for a ribozyme has been defined. Shifted nucleotide pK(a) values have been found to be surprisingly frequent structural elements. Finally, the dynamic na...

journal_title：Current opinion in structural biology

authors： Butcher SE

更新日期：2001-06-01 00:00:00

abstract：:Recent advances in algorithmic techniques and statistics of sequence alignment have helped to make near-optimal alignments an important tool in the delineation of biologically valid alignments. Near-optimal alignments serve to judge the reliability of portions of a sequence alignment. The statistical assessment of sub...

journal_title：Current opinion in structural biology

authors： Vingron M

更新日期：1996-06-01 00:00:00

abstract：:Biology can teach the physical world of electronics, computing, materials science and manufacturing how to assemble complex functional devices and systems that operate at the molecular level. Our present capability to fabricate simple molecular tools, devices, materials and machines is primitive compared with the soph...

journal_title：Current opinion in structural biology

authors： Lowe CR

更新日期：2000-08-01 00:00:00

abstract：:As the fundamental packing units of DNA in eukaryotes, nucleosomes play a central role in governing DNA accessibility in a variety of cellular processes. Our understanding of the mechanisms underlying this complex regulation has been aided by unique structural and dynamic perspectives offered by single molecule techni...

journal_title：Current opinion in structural biology

authors： Killian JL,Li M,Sheinin MY,Wang MD

更新日期：2012-02-01 00:00:00

abstract：:Recent advances in ab initio direct methods have enabled the solution of crystal structures of small proteins from native X-ray data alone, that is, without the use of fragments of known structure or the need to prepare heavy-atom or selenomethionine derivatives, provided that the data are available to atomic resoluti...

journal_title：Current opinion in structural biology

authors： Usón I,Sheldrick GM

更新日期：1999-10-01 00:00:00