Clamp loaders and sliding clamps.

abstract：:Intrinsically disordered regions (IDRs) are fundamental units of protein function and regulation. Despite their inability to form a unique stable tertiary structure in isolation, many IDRs adopt a defined conformation upon binding and achieve their function through their interactions with other biomolecules. However, ...

journal_title：Current opinion in structural biology

authors： Flock T,Weatheritt RJ,Latysheva NS,Babu MM

更新日期：2014-06-01 00:00:00

abstract：:DNA photolyases are extremely efficient light-driven DNA repair enzymes that use the energy of a blue-light photon to 'inject' an electron onto UV-damaged DNA, catalyzing the splitting of mutagenic pyrimidine dimers. By contrast, cryptochromes use blue light to trigger signaling cascades in multicellular organisms, fu...

journal_title：Current opinion in structural biology

authors： Essen LO

更新日期：2006-02-01 00:00:00

abstract：:ATP-binding cassette (ABC) transporters are central to many physiological processes, including the uptake of nutrients, the non-classical secretion of signaling molecules and toxins, multidrug resistance and the development of human disease. As one might expect from this spectrum of translocation events, these ubiquit...

journal_title：Current opinion in structural biology

authors： Schmitt L,Tampé R

更新日期：2002-12-01 00:00:00

abstract：:Magic-angle-spinning (MAS) solid-state NMR spectroscopy is a versatile technique to elucidate functionally important protein-ligand interactions in lipid membranes. Here, we review recent solid-state NMR studies of membrane protein interactions with cholesterol, lipids, transported substrates, and peptide ligands. The...

journal_title：Current opinion in structural biology

authors： Elkins MR,Hong M

更新日期：2019-08-01 00:00:00

abstract：:Proteins are chief actors in life that perform a myriad of exquisite functions. This diversity has been enabled through the evolution and diversification of protein folds. Analysis of sequences and structures strongly suggest that numerous protein pieces have been reused as building blocks and propagated to many moder...

journal_title：Current opinion in structural biology

authors： Romero-Romero S,Kordes S,Michel F,Höcker B

更新日期：2021-01-13 00:00:00

abstract：:Biology can teach the physical world of electronics, computing, materials science and manufacturing how to assemble complex functional devices and systems that operate at the molecular level. Our present capability to fabricate simple molecular tools, devices, materials and machines is primitive compared with the soph...

journal_title：Current opinion in structural biology

authors： Lowe CR

更新日期：2000-08-01 00:00:00

abstract：:The small multidrug resistance proteins constitute a family of bacterial antiporters that confer multidrug resistance by H(+)-linked drug efflux across the bacterial cytoplasmic membrane. The structure of EmrE, the family archetype, has been determined by electron crystallography and shows that EmrE in the membrane is...

journal_title：Current opinion in structural biology

authors： Tate CG

更新日期：2006-08-01 00:00:00

abstract：:G-protein-coupled receptors (GPCRs) participate in virtually all physiological processes. They constitute the largest and most structurally conserved family of signaling molecules. Several class C GPCRs have been shown to exist as dimers in their active form and growing evidence indicates that many, if not all, class ...

journal_title：Current opinion in structural biology

authors： Fotiadis D,Jastrzebska B,Philippsen A,Müller DJ,Palczewski K,Engel A

更新日期：2006-04-01 00:00:00

abstract：:Molecular dynamics simulation studies of protein-nucleic acid complexes are more complicated than studies of either component alone-the force field has to be properly balanced, the systems tend to become very large, and a careful treatment of solvent and of electrostatic interactions is necessary. Recent investigation...

journal_title：Current opinion in structural biology

authors： Mackerell AD Jr,Nilsson L

更新日期：2008-04-01 00:00:00

abstract：:Over the past two decades, mass spectrometry (MS) has emerged as a bone fide approach for structural biology. MS can inform on all levels of protein organization, and enables quantitative assessments of their intrinsic dynamics. The key advantages of MS are that it is a sensitive, high-resolution separation technique ...

journal_title：Current opinion in structural biology

authors： Benesch JL,Ruotolo BT

更新日期：2011-10-01 00:00:00

abstract：:Since user-friendly atomic force microscopes came onto the market a few years ago, scientists have explored the response of many proteins to applied force. This field has now matured beyond the phenomenological with exciting recent developments, particularly with regards to research into biological questions. For exam...

journal_title：Current opinion in structural biology

authors： Forman JR,Clarke J

更新日期：2007-02-01 00:00:00

abstract：:Over recent years, nuclear magnetic resonance (NMR) spectroscopy has developed into a powerful mechanistic tool for the investigation of G protein-coupled receptors (GPCRs). NMR provides insights which underpin the dynamic nature of these important receptors and reveals experimental evidence for a complex conformation...

journal_title：Current opinion in structural biology

authors： Bostock MJ,Solt AS,Nietlispach D

更新日期：2019-08-01 00:00:00

abstract：:Given the increasing interest in protein-protein interactions, the prediction of these interactions from sequence and structural information has become a booming activity. CAPRI, the community-wide experiment for assessing blind predictions of protein-protein interactions, is playing an important role in fostering pro...

journal_title：Current opinion in structural biology

authors： Wodak SJ,Méndez R

更新日期：2004-04-01 00:00:00

abstract：:Channelrhodopsins have become an integral part of modern neuroscience approaches due to their ability to control neuronal activity in targeted cell populations. The recent determination of several channelrhodopsin X-ray structures now enables us to study their function with unprecedented molecular precision. We will d...

journal_title：Current opinion in structural biology

authors： Rappleye M,Berndt A

更新日期：2019-08-01 00:00:00

abstract：:Advances in the crystallization of biological macromolecules have come not only from the application of biochemical, molecular biological and immunological principles and techniques, but also from continued efforts to understand the crystallization process. Developments in crystallization methodologies, protocols, and...

journal_title：Current opinion in structural biology

authors： Gilliland GL,Ladner JE

更新日期：1996-10-01 00:00:00

abstract：:The amide bond with its planarity and lack of chemical reactivity is at the heart of protein structure. Chemical methylation of amides is known but was considered too harsh to be accessible to biology. Until last year there was no protein structure in the data bank with an enzymatically methylated amide. The discovery...

journal_title：Current opinion in structural biology

authors： Song H,Naismith JH

更新日期：2020-12-01 00:00:00

abstract：:Phage display is the longest-standing platform among molecular display technologies. Recent developments have extended its utility to proteins that were previously recalcitrant to phage display. The technique has played a dominant role in forming the field of synthetic binding protein engineering, where novel interfac...

journal_title：Current opinion in structural biology

authors： Sidhu SS,Koide S

更新日期：2007-08-01 00:00:00

abstract：:Recent developments in experimental and computational aspects of NMR spectroscopy have had a significant impact on the accuracy and speed of macromolecular structure determination in solution, particularly with regard to systems of high complexity (such as protein complexes). These include experiments designed to prov...

journal_title：Current opinion in structural biology

authors： Clore GM,Schwieters CD

更新日期：2002-04-01 00:00:00

abstract：:The 26S proteasome is the essential compartmental protease in eukaryotic cells required for the ubiquitin-dependent clearance of damaged polypeptides and obsolete regulatory proteins. Recently, a combination of high-resolution structural, biochemical, and biophysical studies has provided crucial new insights into the ...

journal_title：Current opinion in structural biology

authors： Greene ER,Dong KC,Martin A

更新日期：2020-04-01 00:00:00

abstract：:Continuing investigations into protein-protein interactions have revealed their key role in regulating a wide range of cellular processes. Although efforts to modulate these interactions are more challenging and much less mature than work on conventional drug discovery pathways, significant progress has been made on s...

journal_title：Current opinion in structural biology

authors： Zhao L,Chmielewski J

更新日期：2005-02-01 00:00:00

abstract：:ATP-dependent initiation factors help process replication origins and coordinate replisome assembly to control the onset of DNA synthesis. Although the specific properties and regulatory mechanisms of initiator proteins can vary greatly between different organisms, certain nucleotide-binding elements and assembly patt...

journal_title：Current opinion in structural biology

authors： Duderstadt KE,Berger JM

更新日期：2013-02-01 00:00:00

abstract：:Specialized metabolic enzymes and metabolite diversity evolve through a variety of mechanisms including promiscuity, changes in substrate specificity, modifications of gene expression and gene duplication. For example, gene duplication and substrate binding site changes led to the evolution of the glucosinolate biosyn...

journal_title：Current opinion in structural biology

authors： Leong BJ,Last RL

更新日期：2017-12-01 00:00:00

abstract：:ATP-binding cassette (ABC)-type ATPases are chemo-mechanical engines for diverse biological pathways. ABC ATPase domains act not only in ABC transporters but also in DNA mismatch, nucleotide excision and double-strand break repair enzymes, as well as in chromosome segregation. Atomic-resolution crystal structures sugg...

journal_title：Current opinion in structural biology

authors： Hopfner KP,Tainer JA

更新日期：2003-04-01 00:00:00

abstract：:Fundamental biological processes are driven by diverse molecular machineries. In recent years, single-molecule fluorescence spectroscopy has matured as a unique tool in biology to study how structural dynamics of molecular complexes drive various biochemical reactions. In this review, we highlight underlying developme...

journal_title：Current opinion in structural biology

authors： Choi J,Grosely R,Puglisi EV,Puglisi JD

更新日期：2019-10-01 00:00:00

abstract：:The spliceosome executes eukaryotic precursor messenger RNA (pre-mRNA) splicing to remove noncoding introns through two sequential transesterification reactions, branching and exon ligation. The fidelity of this process is based on the recognition of the conserved sequences in the intron and dynamic compositional and ...

journal_title：Current opinion in structural biology

authors： Wan R,Bai R,Shi Y

更新日期：2019-12-01 00:00:00

abstract：:Protein design is still a challenging undertaking, often requiring multiple attempts or iterations for success. Typically, the source of failure is unclear, and scoring metrics appear similar between successful and failed cases. Nevertheless, the use of sequence statistics, modularity and symmetry from natural protein...

journal_title：Current opinion in structural biology

authors： Broom A,Trainor K,MacKenzie DW,Meiering EM

更新日期：2016-06-01 00:00:00

abstract：:Advances in electron microscopes, detectors and data processing algorithms have greatly facilitated the structural determination of many challenging integral membrane proteins that have been evasive to crystallization. These breakthroughs facilitate the application and development of various membrane protein solubiliz...

journal_title：Current opinion in structural biology

authors： Autzen HE,Julius D,Cheng Y

更新日期：2019-10-01 00:00:00

abstract：:Photolyases repair cytotoxic and mutagenic UV-induced photolesions in DNA by using an amazing light-dependent repair mechanism. It involves light absorption, electron transfer from an excited reduced and deprotonated FADH(-) to the flipped-out photolesion, followed by the fragmentation of the photolesions. Cryptochrom...

journal_title：Current opinion in structural biology

authors： Müller M,Carell T

更新日期：2009-06-01 00:00:00

abstract：:Eukaryotic mRNAs contain a 5' cap structure that protects the transcript against rapid exonucleolytic degradation. The regulation of cellular mRNA levels therefore depends on a precise control of the mRNA decapping pathways. The major mRNA decapping enzyme in eukaryotic cells is Dcp2. It is regulated by interactions w...

journal_title：Current opinion in structural biology

authors： Wurm JP,Sprangers R

更新日期：2019-12-01 00:00:00

abstract：:The polyadenosine (poly(A)) tail found on the 3'-end of almost all eukaryotic mRNAs is important for mRNA stability and regulation of translation. mRNA 3'-end processing occurs co-transcriptionally and involves more than 20 proteins to specifically recognize the polyadenylation site, cleave the pre-mRNA, add a poly(A)...

journal_title：Current opinion in structural biology

authors： Kumar A,Clerici M,Muckenfuss LM,Passmore LA,Jinek M

更新日期：2019-12-01 00:00:00