Abstract:
:The 26S proteasome is the essential compartmental protease in eukaryotic cells required for the ubiquitin-dependent clearance of damaged polypeptides and obsolete regulatory proteins. Recently, a combination of high-resolution structural, biochemical, and biophysical studies has provided crucial new insights into the mechanisms of this fascinating molecular machine. A multitude of new cryo-electron microscopy structures provided snapshots of the proteasome during ATP-hydrolysis-driven substrate translocation, and detailed biochemical studies revealed the timing of individual degradation steps, elucidating the mechanisms for substrate selection and the commitment to degradation through conformational transitions. It was uncovered how ubiquitin removal from substrates is mechanically coupled to degradation, and cryo-electron tomography studies gave a glimpse of active proteasomes inside the cell, their subcellular localization, and interactions with protein aggregates. Here, we summarize these advances in our mechanistic understanding of the proteasome, with a particular focus on how its structural features and conformational transitions enable the multi-step degradation process.
journal_name
Curr Opin Struct Bioljournal_title
Current opinion in structural biologyauthors
Greene ER,Dong KC,Martin Adoi
10.1016/j.sbi.2019.10.004subject
Has Abstractpub_date
2020-04-01 00:00:00pages
33-41eissn
0959-440Xissn
1879-033Xpii
S0959-440X(19)30114-9journal_volume
61pub_type
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journal_title:Current opinion in structural biology
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