Using natural sequences and modularity to design common and novel protein topologies.

Abstract:

:Protein design is still a challenging undertaking, often requiring multiple attempts or iterations for success. Typically, the source of failure is unclear, and scoring metrics appear similar between successful and failed cases. Nevertheless, the use of sequence statistics, modularity and symmetry from natural proteins, combined with computational design both at the coarse-grained and atomistic levels is propelling a new wave of design efforts to success. Here we highlight recent examples of design, showing how the wealth of natural protein sequence and topology data may be leveraged to reduce the search space and increase the likelihood of achieving desired outcomes.

journal_name

Curr Opin Struct Biol

authors

Broom A,Trainor K,MacKenzie DW,Meiering EM

doi

10.1016/j.sbi.2016.05.007

subject

Has Abstract

pub_date

2016-06-01 00:00:00

pages

26-36

eissn

0959-440X

issn

1879-033X

pii

S0959-440X(16)30042-2

journal_volume

38

pub_type

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