Abstract:
:Generally, non-catalytic carbohydrate binding module (CBM) specificity has been shown to parallel the catalytic activity of the carbohydrate active enzyme (CAZyme) module it is appended to. With the rapid expansion in metagenomic sequence space for the potential discovery of new CBMs in addition to the recent emergence of several new CBM families that display diverse binding profiles and novel functions, elucidating the function of these protein modules has become a much more challenging task. This review summarizes several approaches that have been reported for using primary structure to inform CBM specificity and streamlining their biophysical characterization. In addition we discuss general trends in binding site architecture and several newly identified functions for CBMs. Streams of investigation that will facilitate the development and refinement of sequence-based prediction tools are suggested.
journal_name
Curr Opin Struct Bioljournal_title
Current opinion in structural biologyauthors
Abbott DW,van Bueren ALdoi
10.1016/j.sbi.2014.07.004subject
Has Abstractpub_date
2014-10-01 00:00:00pages
32-40eissn
0959-440Xissn
1879-033Xpii
S0959-440X(14)00076-1journal_volume
28pub_type
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