Bacterial polysaccharide synthesis and export.

abstract：:There are now numerous examples of proteins that are unstructured or only partially structured under physiological conditions and yet are nevertheless functional. Such proteins are especially prevalent in eukaryotes. In many cases, intrinsically disordered proteins adopt folded structures upon binding to their biologi...

journal_title：Current opinion in structural biology

authors： Dyson HJ,Wright PE

更新日期：2002-02-01 00:00:00

abstract：:Almost two decades after their identification, the components of the mitotic checkpoint are finally revealing their structural secrets. The activation of Mad2, a central piece of the checkpoint protein machinery, is linked to the rare ability of this protein to adopt two distinct topologies. Current models of checkpoi...

journal_title：Current opinion in structural biology

authors： Mapelli M,Musacchio A

更新日期：2007-12-01 00:00:00

abstract：:Coiled coils have a rich history in the field of protein design and engineering. Novel structures, such as the first seven-helix coiled coil, continue to provide surprises and insights. Large-scale datasets quantifying the influence of systematic mutations on coiled-coil stability are a valuable new asset to the area....

journal_title：Current opinion in structural biology

authors： Grigoryan G,Keating AE

更新日期：2008-08-01 00:00:00

abstract：:The structure of the visual pigment rhodopsin in the dark state was first investigated by electron microscopy (EM). More recently, rhodopsin has been crystallised in two different space groups--a tetragonal P4(1) crystal form and a trigonal P3(1) packing arrangement. The structures of the pigment, determined by X-ray ...

journal_title：Current opinion in structural biology

authors： Schertler GF

更新日期：2005-08-01 00:00:00

abstract：:The torque-generating, direction-reversing switch proteins of the bacterial flagellar rotary motor form a cytoplasmic extension of the bacterial flagellar basal body. 10 A maps, obtained by electron cryomicroscopy, of the bacterial filament reveal an unusual alpha domain which forms the protein-subunit export channel....

journal_title：Current opinion in structural biology

authors： DeRosier DJ

更新日期：1995-04-01 00:00:00

abstract：:Salt-dependent electrostatic effects are a major factor in determining the stability, structure, reactivity, and binding behavior of nucleic acids. Increasingly detailed theoretical methods, especially those based on Monte Carlo and Poisson-Boltzmann methodologies, combined with powerful computational algorithms are b...

journal_title：Current opinion in structural biology

authors： Sharp KA,Honig B

更新日期：1995-06-01 00:00:00

abstract：:Intermediate filaments are built from one to several members of a multigene family encoding fibrous proteins that share a highly conserved hierarchic assembly plan for the formation of multistranded filaments from distinctly structured extended coiled coils. Despite the rather low primary sequence identity, intermedia...

journal_title：Current opinion in structural biology

authors： Herrmann H,Aebi U

更新日期：1998-04-01 00:00:00

abstract：:The application of biocatalysis for the synthesis of glycans and glycoconjugates is a well-established and successful strategy, both for small and large scale synthesis. Compared to chemical synthesis, is has the advantage of high selectivity, but biocatalysis had been largely limited to natural glycans both in terms ...

journal_title：Current opinion in structural biology

authors： Pallister E,Gray CJ,Flitsch SL

更新日期：2020-12-01 00:00:00

abstract：:Directed evolution is widely used to improve enzymes, particularly for industrial biocatalytic processes. Molecular biology advances present many new strategies for directed evolution. Commonly used techniques have led to many successful examples of enzyme improvement, yet there is still a need to improve both the eff...

journal_title：Current opinion in structural biology

authors： Dalby PA

更新日期：2011-08-01 00:00:00

abstract：:The past twelve months have seen a renewal of interest in the therapeutic potential of members of the tumour necrosis factor receptor family and their cytokine ligands. This biomedical interest has spawned a number of structural studies, which have significantly deepened our understanding of the molecular basis for th...

journal_title：Current opinion in structural biology

authors： Jones EY

更新日期：2000-12-01 00:00:00

abstract：:H+ transporting, reversible F-type ATPases (ATP synthases) and V-type ATPases share major structural features and function by similar mechanisms. Recent structural and genetic experiments provide new insights into the organization of the transmembrane and coupling sectors of the enzymes and the molecular mechanics of ...

journal_title：Current opinion in structural biology

authors： Fillingame RH

更新日期：1996-08-01 00:00:00

abstract：:Computational methods play an important role at all stages of the process of determining protein-protein interactions. They are used to predict potential interactions, to validate the results of high-throughput interaction screens and to analyze the protein networks inferred from interaction databases. ...

journal_title：Current opinion in structural biology

authors： Salwinski L,Eisenberg D

更新日期：2003-06-01 00:00:00

abstract：:Protein-protein interactions involving the catalytic domain of protein kinases are likely to be generally important in the regulation of signal transduction pathways, but are rather sparsely represented in crystal structures. Recently determined structures of the kinase domains of the mitogen-activated protein kinase ...

journal_title：Current opinion in structural biology

authors： Pellicena P,Kuriyan J

更新日期：2006-12-01 00:00:00

abstract：:The radical intermediates generated during the catalytic cycles of adenosylcobalamin-dependent enzymes occur in pairs. The positions of radicals residing on the cofactor, substrate or protein, relative to the position of the low-spin Co(2+) from the cob(II)alamin intermediate, can be extracted from electron paramagnet...

journal_title：Current opinion in structural biology

authors： Reed GH,Mansoorabadi SO

更新日期：2003-12-01 00:00:00

abstract：:The past two years have seen the extension of our knowledge on the cellular prion protein structure with new NMR data on both the hamster and human proteins. In addition, the folding dynamics of two cellular prion proteins have been elucidated. There are now several examples of recombinant prion proteins that are able...

journal_title：Current opinion in structural biology

authors： Jackson GS,Clarke AR

更新日期：2000-02-01 00:00:00

abstract：:The most important advances in the field of genome annotation over the past two years involve the use of cDNA sequences, protein structures and gene expression data to predict genes. These types of information not only improve gene identification, but they also give insights into variation in gene structure and functi...

journal_title：Current opinion in structural biology

authors： Gaasterland T,Oprea M

更新日期：2001-06-01 00:00:00

abstract：:Structural modeling of a cell is an evolving strategic direction in computational structural biology. It takes advantage of new powerful modeling techniques, deeper understanding of fundamental principles of molecular structure and assembly, and rapid growth of the amount of structural data generated by experimental t...

journal_title：Current opinion in structural biology

authors： Vakser IA,Deeds EJ

更新日期：2019-04-01 00:00:00

abstract：:Protein design efforts are beginning to yield molecules with many of the properties of natural proteins. Such experiments are informed by and contribute to our understanding of the sequence determinants of protein folding and stability. The most important design elements seem to be the proper placement of hydrophobic ...

journal_title：Current opinion in structural biology

authors： Cordes MH,Davidson AR,Sauer RT

更新日期：1996-02-01 00:00:00

abstract：:Protein-RNA interactions play essential roles in gene regulation and RNA metabolism. While high-resolution structures have revealed principles of RNA recognition by individual RNA binding domains (RBDs), the presence of multiple RBDs in many eukaryotic proteins suggests additional modes of RNA recognition by combinati...

journal_title：Current opinion in structural biology

authors： Mackereth CD,Sattler M

更新日期：2012-06-01 00:00:00

abstract：:Since user-friendly atomic force microscopes came onto the market a few years ago, scientists have explored the response of many proteins to applied force. This field has now matured beyond the phenomenological with exciting recent developments, particularly with regards to research into biological questions. For exam...

journal_title：Current opinion in structural biology

authors： Forman JR,Clarke J

更新日期：2007-02-01 00:00:00

abstract：:The postsynaptic density (PSD) is a protein-rich assembly below the postsynaptic membrane, formed of large scaffolding proteins. These proteins carry a combination of protein interaction domains, which may interact with several alternative partners; the structure of the protein assembly can be regulated in an activity...

journal_title：Current opinion in structural biology

authors： Kursula P

更新日期：2019-02-01 00:00:00

abstract：:Emerging data suggest that the mechanisms by which RNA-binding proteins (RBPs) interact with RNA and the rules governing specificity might be substantially more complex than those underlying their DNA-binding counterparts. Even our knowledge of what constitutes the RNA-bound proteome is contentious; recent studies sug...

journal_title：Current opinion in structural biology

authors： Helder S,Blythe AJ,Bond CS,Mackay JP

更新日期：2016-06-01 00:00:00

abstract：:Antibody engineering has been an extremely intensive research area for many years. Recent achievements discussed in this review include: (i) significant improvements in the field of selection of antigen-specific antibody fragments on bacteriophages; (ii) new structural work, in particular using NMR; (iii) the cloning ...

journal_title：Current opinion in structural biology

authors： Nilsson B

更新日期：1995-08-01 00:00:00

abstract：:Biology has, and continues to be, shaped by evolutionary mechanisms. Within the past decade, local fitness landscapes have become experimentally tractable and are providing new perspectives on evolutionary mechanisms. Powered by next-generation sequencing, the impacts of all individual amino acid substitutions on func...

journal_title：Current opinion in structural biology

authors： Canale AS,Cote-Hammarlof PA,Flynn JM,Bolon DN

更新日期：2018-02-01 00:00:00

abstract：:The three-dimensional structures of several biotin-binding proteins are now known, giving insights into the molecular architecture of the binding sites for biotin. In combination with biochemical and computational approaches, these structural insights provide the basis for our present understanding of biotin-protein i...

journal_title：Current opinion in structural biology

authors： Lindqvist Y,Schneider G

更新日期：1996-12-01 00:00:00

abstract：:Channel-forming bacterial toxins undergo a series of remarkable changes in solubility, oligomerization state, structure and dynamics during the processes of membrane binding, assembly, membrane insertion and channel formation. Recent high-resolution crystal structures of channel-forming toxins, in both water-soluble a...

journal_title：Current opinion in structural biology

authors： Gouaux E

更新日期：1997-08-01 00:00:00

abstract：:The spliceosome executes eukaryotic precursor messenger RNA (pre-mRNA) splicing to remove noncoding introns through two sequential transesterification reactions, branching and exon ligation. The fidelity of this process is based on the recognition of the conserved sequences in the intron and dynamic compositional and ...

journal_title：Current opinion in structural biology

authors： Wan R,Bai R,Shi Y

更新日期：2019-12-01 00:00:00

abstract：:This review focuses on pharmacophore approaches in researching protein interfaces that bind protein ligands. Pharmacophore descriptions of binding interfaces that employ molecular dynamics simulation can account for effects of solvation and conformational flexibility. In addition, these calculations provide an approxi...

journal_title：Current opinion in structural biology

authors： Shrestha R,Fajardo JE,Fiser A

更新日期：2021-01-21 00:00:00

abstract：:Protein engineering strategies for increasing stability can be improved by replacing random mutagenesis and high-throughput screening by approaches that include bioinformatics and computational design. Mutations can be focused on regions in the structure that are most flexible and involved in the early steps of therma...

journal_title：Current opinion in structural biology

authors： Wijma HJ,Floor RJ,Janssen DB

更新日期：2013-08-01 00:00:00

abstract：:Transmembrane helix-helix interactions mediate the folding and assembly of membrane proteins. Recognition motifs range from GxxxG and leucine zippers to polar side chains and salt bridges. Some canonical membrane proteins contain local charge clusters that are important for folding and function, and which have to be c...

journal_title：Current opinion in structural biology

authors： Walther TH,Ulrich AS

更新日期：2014-08-01 00:00:00