Opportunities for structure-based design of protease-directed drugs.

abstract：:Recent developments in implicit solvent models may be compared in terms of accuracy and computational efficiency. Based on improvements in the accuracy of generalized Born methods and the speed of Poisson-Boltzmann solvers, it appears that the two techniques are converging to a point at which both will be suitable for...

journal_title：Current opinion in structural biology

authors： Baker NA

更新日期：2005-04-01 00:00:00

abstract：:Aromatic polyketides are secondary metabolites that afford some of the most common antibiotics and anti-cancer drugs currently in clinical use. Not least because of their medical importance, the biosynthesis of these compounds has attracted considerable interest during the past few years; important advances have been ...

journal_title：Current opinion in structural biology

authors： Schneider G

更新日期：2005-12-01 00:00:00

abstract：:Electrostatics plays a major role in the stabilization and function of biomolecules; as such, it remains a major focus of theoretical and computational studies of macromolecules. Electrostatic interactions are long range, and strongly dependent on the solvent and ions surrounding the biomolecule under study. During th...

journal_title：Current opinion in structural biology

authors： Koehl P

更新日期：2006-04-01 00:00:00

abstract：:A network of dynamic protein interactions with their protein partners, substrates, and ligands is known to be crucial for biological function. Revealing molecular and structural-based mechanisms at atomic resolution and in real-time is fundamental for achieving a basic understanding of cellular processes. These techni...

journal_title：Current opinion in structural biology

authors： Grossman M,Sela-Passwell N,Sagi I

更新日期：2011-10-01 00:00:00

abstract：:RNA motifs are directed and ordered stacked arrays of non-Watson-Crick base pairs forming distinctive foldings of the phosphodiester backbones of the interacting RNA strands. They correspond to the 'loops' - hairpin, internal and junction - that intersperse the Watson-Crick two-dimensional helices as seen in two-dimen...

journal_title：Current opinion in structural biology

authors： Leontis NB,Westhof E

更新日期：2003-06-01 00:00:00

abstract：:Intrinsically disordered proteins (IDPs) are increasingly realized to play diverse biological roles, ranging from molecular signaling to the formation of membraneless organelles. Their high degree of disorder makes them more challenging to study using the techniques of conventional structural biology, because any obse...

journal_title：Current opinion in structural biology

authors： Best RB

更新日期：2017-02-01 00:00:00

abstract：:Structures of two restriction endonucleases, BamHI and PvuII, were reported in the past year. This doubles the number of restriction endonuclease structures now known from two to four, and enables a comparative analysis of their structures and modes of DNA recognition. Despite a lack of sequence homology between the e...

journal_title：Current opinion in structural biology

authors： Aggarwal AK

更新日期：1995-02-01 00:00:00

abstract：:The recently determined structure of octameric mitochondrial creatine kinase has provided new insights into the functioning of this enzyme and its role in channelling energy from the mitochondria to the cytoplasm. Creatine kinase, a member of the family of guanidino kinases, is structurally similar to glutamine synthe...

journal_title：Current opinion in structural biology

authors： Kabsch W,Fritz-Wolf K

更新日期：1997-12-01 00:00:00

abstract：:Ribonuclease P (RNase P) is one of only two known universal ribozymes and was one of the first ribozymes to be discovered. It is involved in RNA processing, in particular the 5' maturation of tRNA. Unlike most other natural ribozymes, it recognizes and cleaves its substrate in trans. RNase P is a ribonucleoprotein com...

journal_title：Current opinion in structural biology

authors： Torres-Larios A,Swinger KK,Pan T,Mondragón A

更新日期：2006-06-01 00:00:00

abstract：:Transition states in protein folding are being analyzed experimentally and by computer simulation to reveal the sequence of events in the folding and unfolding of proteins at high resolution. Results of this analysis have enabled the reconstruction of folding pathways in vitro and open the prospect of analyzing pathwa...

journal_title：Current opinion in structural biology

authors： Fersht AR

更新日期：1995-02-01 00:00:00

abstract：:The most important advances in the field of genome annotation over the past two years involve the use of cDNA sequences, protein structures and gene expression data to predict genes. These types of information not only improve gene identification, but they also give insights into variation in gene structure and functi...

journal_title：Current opinion in structural biology

authors： Gaasterland T,Oprea M

更新日期：2001-06-01 00:00:00

abstract：:Specific, noncovalent binding of biomolecules can only be understood by considering structural, thermodynamic, and kinetic issues. The theoretical foundations for such analyses have been clarified in the past year. Computational techniques for both particle-based and continuum models continue to improve and to yield u...

journal_title：Current opinion in structural biology

authors： McCammon JA

更新日期：1998-04-01 00:00:00

abstract：:The postsynaptic density (PSD) is a protein-rich assembly below the postsynaptic membrane, formed of large scaffolding proteins. These proteins carry a combination of protein interaction domains, which may interact with several alternative partners; the structure of the protein assembly can be regulated in an activity...

journal_title：Current opinion in structural biology

authors： Kursula P

更新日期：2019-02-01 00:00:00

abstract：:Complementary developments in comparative genomics, protein structure determination and in-depth comparison of protein sequences and structures have provided a better understanding of the prevailing trends in the emergence and diversification of protein domains. The investigation of deep relationships among different ...

journal_title：Current opinion in structural biology

authors： Aravind L,Mazumder R,Vasudevan S,Koonin EV

更新日期：2002-06-01 00:00:00

abstract：:The past twelve months have seen a renewal of interest in the therapeutic potential of members of the tumour necrosis factor receptor family and their cytokine ligands. This biomedical interest has spawned a number of structural studies, which have significantly deepened our understanding of the molecular basis for th...

journal_title：Current opinion in structural biology

authors： Jones EY

更新日期：2000-12-01 00:00:00

abstract：:Membranes are sites of intense signaling activity within the cell, serving as dynamic scaffolds for the recruitment of signaling molecules and their substrates. The specific and reversible localization of these signaling molecules to membranes is critical for the appropriate activation of downstream signaling pathways...

journal_title：Current opinion in structural biology

authors： Leonard TA,Hurley JH

更新日期：2011-12-01 00:00:00

abstract：:During protein evolution, novel domain arrangements are continuously formed. Rearrangements are important for the creation of molecular biodiversity and for functional molecular changes which underlie developmental shifts in the bauplan of organisms. Here we review the mechanisms by which new arrangements arise and th...

journal_title：Current opinion in structural biology

authors： Bornberg-Bauer E,Albà MM

更新日期：2013-06-01 00:00:00

abstract：:Coiled coils have a rich history in the field of protein design and engineering. Novel structures, such as the first seven-helix coiled coil, continue to provide surprises and insights. Large-scale datasets quantifying the influence of systematic mutations on coiled-coil stability are a valuable new asset to the area....

journal_title：Current opinion in structural biology

authors： Grigoryan G,Keating AE

更新日期：2008-08-01 00:00:00

abstract：:The recent and growing evidence that the efficacy of a drug can be correlated to target binding kinetics has seeded the development of a multitude of novel methods aimed at computing rate constants for receptor-ligand binding processes, as well as gaining an understanding of the binding and unbinding pathways and the ...

journal_title：Current opinion in structural biology

authors： Bruce NJ,Ganotra GK,Kokh DB,Sadiq SK,Wade RC

更新日期：2018-04-01 00:00:00

abstract：:During the past few years, there have been exciting developments in the field of flavoenzymology. New flavoenzymes have been discovered that are implicated in a variety of biological processes, including cell signaling, chromatin remodeling and cell development. The structures of several of these new flavoenzymes have...

journal_title：Current opinion in structural biology

authors： De Colibus L,Mattevi A

更新日期：2006-12-01 00:00:00

abstract：:Mucin-type O-glycosylation is a post-translational modification (PTM) that is predicted to occur in more than the 80% of the proteins that pass through the Golgi apparatus. This PTM is initiated by a family of polypeptide GalNAc-transferases (GalNAc-Ts) that modify Ser and Thr residues of proteins through the addition...

journal_title：Current opinion in structural biology

authors： de Las Rivas M,Lira-Navarrete E,Gerken TA,Hurtado-Guerrero R

更新日期：2019-06-01 00:00:00

abstract：:There are now numerous examples of proteins that are unstructured or only partially structured under physiological conditions and yet are nevertheless functional. Such proteins are especially prevalent in eukaryotes. In many cases, intrinsically disordered proteins adopt folded structures upon binding to their biologi...

journal_title：Current opinion in structural biology

authors： Dyson HJ,Wright PE

更新日期：2002-02-01 00:00:00

abstract：:Improved rational drug design methods are needed to lower the cost and increase the success rate of drug discovery and development. Alchemical binding free energy calculations, one potential tool for rational design, have progressed rapidly over the past decade, but still fall short of providing robust tools for pharm...

journal_title：Current opinion in structural biology

authors： Chodera JD,Mobley DL,Shirts MR,Dixon RW,Branson K,Pande VS

更新日期：2011-04-01 00:00:00

abstract：:Photolyases repair cytotoxic and mutagenic UV-induced photolesions in DNA by using an amazing light-dependent repair mechanism. It involves light absorption, electron transfer from an excited reduced and deprotonated FADH(-) to the flipped-out photolesion, followed by the fragmentation of the photolesions. Cryptochrom...

journal_title：Current opinion in structural biology

authors： Müller M,Carell T

更新日期：2009-06-01 00:00:00

abstract：:For various reasons, it seems sensible to redesign or design proteins from the inside out. Past approaches in this field have involved iterations of mutagenesis and characterisation to 'evolve' designs. Increasingly, combinatorial approaches are being taken to select 'fit' sequences from libraries of variant proteins....

journal_title：Current opinion in structural biology

authors： Woolfson DN

更新日期：2001-08-01 00:00:00

abstract：:Optogenetic dimerizers are modular domains that can be utilized in a variety of versatile ways to modulate cellular biochemistry. Because of their modularity, many applications using these tools can be easily transferred to new targets without extensive engineering. While a number of photodimerizer systems are current...

journal_title：Current opinion in structural biology

authors： Spiltoir JI,Tucker CL

更新日期：2019-08-01 00:00:00

abstract：:Fucose is a common terminal modification on protein and lipid glycans. Fucose can also be directly linked to protein via an O-linkage to Serine or Threonine residues located within consensus sequences contained in Epidermal Growth Factor-like (EGF) repeats and Thrombospondin Type 1 Repeats (TSRs). In this context, fuc...

journal_title：Current opinion in structural biology

authors： Holdener BC,Haltiwanger RS

更新日期：2019-06-01 00:00:00

abstract：:Synapses are cell adhesion structures specialized for signal transmission between neurons. At the synapse, presynaptic and postsynaptic terminals of neurons are functionally connected but spatially separated and form a cleft. Membrane receptor-like cell adhesion molecules and secreted proteins in the synaptic cleft (s...

journal_title：Current opinion in structural biology

authors： Yamagata A,Fukai S

更新日期：2019-02-01 00:00:00

abstract：:Magic-angle-spinning (MAS) solid-state NMR spectroscopy is a versatile technique to elucidate functionally important protein-ligand interactions in lipid membranes. Here, we review recent solid-state NMR studies of membrane protein interactions with cholesterol, lipids, transported substrates, and peptide ligands. The...

journal_title：Current opinion in structural biology

authors： Elkins MR,Hong M

更新日期：2019-08-01 00:00:00

abstract：:Antibody engineering has been an extremely intensive research area for many years. Recent achievements discussed in this review include: (i) significant improvements in the field of selection of antigen-specific antibody fragments on bacteriophages; (ii) new structural work, in particular using NMR; (iii) the cloning ...

journal_title：Current opinion in structural biology

authors： Nilsson B

更新日期：1995-08-01 00:00:00