Abstract:
:Condensation of DNA in the nucleosome takes advantage of its double-helical architecture. The DNA deforms at sites where the base pairs face the histone octamer. The largest so-called kink-and-slide deformations occur in the vicinity of arginines that penetrate the minor groove. Nucleosome structures formed from the 601 positioning sequence differ subtly from those incorporating an AT-rich human α-satellite DNA. Restraints imposed by the histone arginines on the displacement of base pairs can modulate the sequence-dependent deformability of DNA and potentially contribute to the unique features of the different nucleosomes. Steric barriers mimicking constraints found in the nucleosome induce the simulated large-scale rearrangement of canonical B DNA to kink-and-slide states. The pathway to these states shows nonharmonic behavior consistent with bending profiles inferred from AFM measurements.
journal_name
Curr Opin Struct Bioljournal_title
Current opinion in structural biologyauthors
Olson WK,Zhurkin VBdoi
10.1016/j.sbi.2011.03.006subject
Has Abstractpub_date
2011-06-01 00:00:00pages
348-57issue
3eissn
0959-440Xissn
1879-033Xpii
S0959-440X(11)00042-Xjournal_volume
21pub_type
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journal_title:Current opinion in structural biology
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