Abstract:
:Heparan sulfate (HS) is a ubiquitous glycosaminoglycan that serves as a cellular attachment site for a number of significant human pathogens, including respiratory syncytial virus (RSV), human parainfluenza virus 3 (hPIV3), and herpes simplex virus (HSV). Decoy receptors can target pathogens by binding to the receptor pocket on viral attachment proteins, acting as 'molecular sinks' and preventing the pathogen from binding to susceptible host cells. Decoy receptors functionalized with HS could bind to pathogens and prevent infection, so we generated decoy liposomes displaying HS-octasaccharide (HS-octa). These decoy liposomes significantly inhibited RSV, hPIV3, and HSV infectivity in vitro to a greater degree than the original HS-octa building block. The degree of inhibition correlated with the density of HS-octa displayed on the liposome surface. Decoy liposomes with HS-octa inhibited infection of viruses to a greater extent than either full-length heparin or HS-octa alone. Decoy liposomes were effective when added prior to infection or following the initial infection of cells in vitro. By targeting the well-conserved receptor-binding sites of HS-binding viruses, decoy liposomes functionalized with HS-octa are a promising therapeutic antiviral agent and illustrate the utility of the liposome delivery platform.
journal_name
Antiviral Resjournal_title
Antiviral researchauthors
Hendricks GL,Velazquez L,Pham S,Qaisar N,Delaney JC,Viswanathan K,Albers L,Comolli JC,Shriver Z,Knipe DM,Kurt-Jones EA,Fygenson DK,Trevejo JM,Wang JP,Finberg RWdoi
10.1016/j.antiviral.2015.01.008subject
Has Abstractpub_date
2015-04-01 00:00:00pages
34-44eissn
0166-3542issn
1872-9096pii
S0166-3542(15)00009-1journal_volume
116pub_type
杂志文章abstract::The bovine immunodeficiency virus (BIV) and human immunodeficiency virus types 1 and 2 (HIV-1 and -2) are members of the lentivirus genus of retroviruses. Although DNA sequences of these viruses have diverged considerably, the BIV genome organization, function of structural and regulatory genes, and replication cycle ...
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journal_title:Antiviral research
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
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更新日期:2003-06-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2003-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:2015-06-01 00:00:00
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更新日期:2006-10-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1016/j.antiviral.2007.09.001
更新日期:2008-02-01 00:00:00
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pub_type: 杂志文章,随机对照试验
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pub_type: 杂志文章
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