Abstract:
:Classical swine fever virus (CSFV) is the causative pathogen of classical swine fever (CSF), a highly contagious disease of swine. Mx proteins are interferon-induced dynamin-like GTPases present in all vertebrates with a wide range of antiviral activities. Although Zhao et al. (2011) have reported that human MxA can inhibit CSFV replication, whether porcine Mx1 (poMx1) has anti-CSFV activity remains unknown. In this study, we generated a cell line designated PK-15/EGFP-poMx1 which expressed porcine Mx1 protein constitutively, and we observed that the proliferation of progeny virus in this cell line was significantly inhibited as measured by virus titration, indirect immune fluorescence assay, Q-PCR and Western blot. Furthermore, when PTD-poMx1 fusion protein expressed in Escherichia coli (Zhang et al., 2013) was used to treat CSFV-infected PK-15 cells, the results showed that PTD-poMx1 inhibited CSFV replication in a dose-dependent manner. Additionally, the proliferation of progeny virus was inhibited as measured by virus titration and Q-PCR. Overall, the results demonstrated that poMx1 effectively inhibited CSFV replication, suggesting that poMx1 may be a valuable therapeutic agent against CSFV infection.
journal_name
Antiviral Resjournal_title
Antiviral researchauthors
He DN,Zhang XM,Liu K,Pang R,Zhao J,Zhou B,Chen PYdoi
10.1016/j.antiviral.2014.01.020subject
Has Abstractpub_date
2014-04-01 00:00:00pages
128-35eissn
0166-3542issn
1872-9096pii
S0166-3542(14)00032-1journal_volume
104pub_type
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pub_type: 临床试验,杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Antiviral research
pub_type: 杂志文章,评审
doi:10.1016/j.antiviral.2005.05.001
更新日期:2005-08-01 00:00:00
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journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2006.12.003
更新日期:2007-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:1987-03-01 00:00:00