Optical imaging of COX-2: studies on an autofluorescent 2,3-diaryl-substituted indole-based cyclooxygenase-2 inhibitor.

abstract：:Osteoarthritis (OA) is the most common form of joint disease. The development of inflammation have been considered to play a key role during the progression of OA. Regulatory pathways are known to play crucial roles in many pathogenic processes. Thus, deciphering these risk regulatory pathways is critical for elucidat...

journal_title：Biochemical and biophysical research communications

authors： Song Z,Zhang C,He L,Sui Y,Lin X,Pan J

更新日期：2018-06-12 00:00:00

abstract：:Arthritis is a common autoimmune disease that is associated with progressive disability, systemic complications and early death. However, the underling mechanisms of arthritis are still unclear. Sirtuins are a NAD(+)-dependent class III deacetylase family, and regulate cellular stress, inflammation, genomic stability,...

journal_title：Biochemical and biophysical research communications

authors： Lin J,Sun B,Jiang C,Hong H,Zheng Y

更新日期：2013-11-29 00:00:00

abstract：:OPA1 is a cause gene for autosomal dominant optic atrophy and possesses eight alternative splicing variants. Here, we identified two isoforms of OPA1 proteins in HeLa cells and examined their submitochondrial localization and complex formations. RT-PCR shows that HeLa cells mainly express isoforms 7 and 1 of OPA1. Sin...

journal_title：Biochemical and biophysical research communications

authors： Satoh M,Hamamoto T,Seo N,Kagawa Y,Endo H

更新日期：2003-01-10 00:00:00

abstract：:Groucho is a corepressor that forms a macromolecular complex for its corepressor activity, in which HDAC1 is an essential component for the modulation of chromatin structure and transcriptional repression of target genes. Here, we show that Groucho is covalently conjugated with small ubiquitin-related modifier-1 (SUMO...

journal_title：Biochemical and biophysical research communications

authors： Ahn JW,Lee YA,Ahn JH,Choi CY

更新日期：2009-01-30 00:00:00

abstract：:Our previous study on the dynamic transcriptomes activated during human erythropoiesis suggested that transcription factor forkhead box O3 (FOXO3) possibly plays a role in erythroid differentiation. Functional studies in human cell line TF-1 indicated that FOXO3 knockdown repressed erythropoietin (EPO)-induced erythro...

journal_title：Biochemical and biophysical research communications

authors： Wang H,Li Y,Wang S,Zhang Q,Zheng J,Yang Y,Qi H,Qu H,Zhang Z,Liu F,Fang X

更新日期：2015-05-15 00:00:00

abstract：:Heat shock proteins (HSPs) are involved in protein folding, aggregation, transport and/or stabilization by acting as a molecular chaperone, leading to inhibition of apoptosis by both caspase dependent and/or independent pathways. HSPs are overexpressed in a wide range of human cancers and are implicated in tumor cell ...

journal_title：Biochemical and biophysical research communications

authors： Sarkar R,Mukherjee S,Biswas J,Roy M

更新日期：2012-10-12 00:00:00

abstract：:Viral vectors displaying specific ligand binding moieties have raised an increasing interest in the area of targeted gene therapy. In this report, we describe baculovirus vectors displaying either a functional single chain antibody fragment (scFv) specific for the carcinoembryonic antigen (CEA) or the synthetic IgG bi...

journal_title：Biochemical and biophysical research communications

authors： Ojala K,Mottershead DG,Suokko A,Oker-Blom C

更新日期：2001-06-15 00:00:00

abstract：:Plasminogen activation by tissue-plasminogen activator (t-PA) is accelerated by the presence of a macromolecular surface, which acts as a template that brings enzyme and substrate in close proximity. Modification of lysine residues, which are important for this template function, occurs in diabetic patients as a conse...

journal_title：Biochemical and biophysical research communications

authors： Bobbink IW,Tekelenburg WL,Sixma JJ,de Boer HC,Banga JD,de Groot PG

更新日期：1997-11-26 00:00:00

abstract：:Neurogenin-3 (ngn-3) expression is critical for endocrine development in the developing pancreas. We found that when ngn-3 was inhibited in an E11.5 pancreas, using either morpholino antisense or siRNA, it led to a significant decrease in endocrine differentiation after seven days in culture. Endocrine differentiation...

journal_title：Biochemical and biophysical research communications

authors： Prasadan K,Tulachan S,Guo P,Shiota C,Shah S,Gittes G

更新日期：2010-06-11 00:00:00

abstract：:N-Palmitoyl-(S)-seryl-(S)-seryl-(S)-asparaginyl-(S)-alanine (Palmitoyl-tetrapeptide) is an analogue of the N-terminal part of the lipoprotein from the outer membrane of Escherichia coli. It was prepared by chemical synthesis and tested for biological activity in in vitro lymphocyte culture systems. In spleen cells of ...

journal_title：Biochemical and biophysical research communications

authors： Bessler WG,Cox M,Wiesmüller KH,Jung G

更新日期：1984-05-31 00:00:00

abstract：:A new gene (GenBank Accession No. AF114264) was cloned from umbilical vein wall tissue by using RT-PCR. The gene shares high similarity to the gene encoding F-actin binding protein nexilin, so named as NELIN. A clone of 2737bp contains open reading frame of 1344bp extending from 412 to 1755. NELIN was expressed primar...

journal_title：Biochemical and biophysical research communications

authors： Wang W,Zhang W,Han Y,Chen J,Wang Y,Zhang Z,Hui R

更新日期：2005-05-20 00:00:00

abstract：:It has been suggested that curcumin and other sarcoplasmic/endoplasmic reticulum Ca(2+)-pump inhibitors could correct the defect in the most common mutation (DeltaF508) in cystic fibrosis (CF), and restore normal chloride transport. In the present study, the effect of curcumin was tested on baby hamster kidney (BHK) c...

journal_title：Biochemical and biophysical research communications

authors： Dragomir A,Björstad J,Hjelte L,Roomans GM

更新日期：2004-09-17 00:00:00

abstract：:We report the discovery, semi-purification and characterization of a novel Ca2+/calmodulin-dependent protein kinase (peak I kinase) using syntide 2 as a substrate from the rabbit heart. In the study of dependence of peak I kinase on the concentration of calmodulin, half-maximal activation was obtained at approx. 2.0 x...

journal_title：Biochemical and biophysical research communications

authors： Uemura A,Okazaki K,Takesue H,Matsubara T,Hidaka H

更新日期：1995-06-15 00:00:00

abstract：:We have selected calcium ionophore (A23187)-resistant cells (AR-7) in a human promyelocytic leukemia cell line, HL-60. AR-7 showed approximately 8.5-fold resistance to A23187 compared with the wild type cells after continuous exposure for 3 days. AR-7 had cross resistance to ionomycin (4.6-fold) and thapsigargin (340-...

journal_title：Biochemical and biophysical research communications

authors： Kubota M,Kataoka A,Okuda A,Bessho R,Lin YW,Wakazono Y,Usami I,Akiyama Y,Furusho K

更新日期：1995-08-15 00:00:00

abstract：:A novel application of atomic force microscopy (AFM) in the rapid, label-free detection and identification of viruses is described. Multiplexed, miniaturized antibody domains were constructed using "ink-jet" protein arraying technology. The solid-phase affinity substrate termed the "ViriChip" was used in the immunocap...

journal_title：Biochemical and biophysical research communications

authors： Nettikadan SR,Johnson JC,Mosher C,Henderson E

更新日期：2003-11-14 00:00:00

abstract：:Fractionation of rat uterine cells incubated at 22 degrees C with 0.2 nM [3H]-estradiol-17 beta (E2 beta) was performed to analyze the subcellular distribution of internalized hormone. The postnuclear supernatant of homogenates was resolved in Percoll density gradients into six major fractions defined by enzyme marker...

journal_title：Biochemical and biophysical research communications

authors： Pietras RJ,Szego CM

更新日期：1984-08-30 00:00:00

abstract：:Reactive oxygen species (ROS) contributes to endothelial dysfunction that is involved in the pathogeneses of hypertension. Vascular peroxidase 1 (VPO1) can utilize ROS to catalyze peroxidative reactions, possibly enhancing endothelial dysfunction. This study is to identify VPO1's involvement in endothelial dysfunction...

journal_title：Biochemical and biophysical research communications

authors： Yang L,Bai Y,Li N,Hu C,Peng J,Cheng G,Zhang G,Shi R

更新日期：2013-10-04 00:00:00

abstract：:Replacement of leucine in the chemotactic peptide For-Met-Leu-Phe by the sterically constrained amino acids alpha-aminoisobutyric acid and aminocyclohexanecarboxylic acid affords compounds of equal or greater activity than the parent. NMR studies indicate that the parent compound is present as a beta-sheet in solution...

journal_title：Biochemical and biophysical research communications

authors： Semus SF,Becker EL,Toniolo C,Freer RJ

更新日期：1988-12-15 00:00:00

abstract：:ABCA1 on the cell surface and in endosomes plays an essential role in the cell-mediated lipidation of apoA-I to form nascent HDL. Our previous studies of transgenic mice overexpressing ABCA1 suggested that ABCA1 in the liver plays a major role in regulating plasma HDL levels. The site of function of ABCA1 in the polar...

journal_title：Biochemical and biophysical research communications

authors： Neufeld EB,Demosky SJ Jr,Stonik JA,Combs C,Remaley AT,Duverger N,Santamarina-Fojo S,Brewer HB Jr

更新日期：2002-10-04 00:00:00

abstract：:The presence of highly specific serum binding proteins for growth hormone (GH) has been well characterized in many species. In the rat the major growth hormone-binding protein (GH-BP) is a truncated, variant form of the target tissue GH receptor and is derived by an alternative mRNA splicing event. The GHBP mRNA is co...

journal_title：Biochemical and biophysical research communications

authors： Tiong TS,Herington AC

更新日期：1991-10-31 00:00:00

abstract：:IL-17, which exerts strong pro-inflammatory effects, has emerged as an important mediator in inflammation-associated cancer. However, the characteristics of IL-17-producing cells, the relevance of IL-17 to clinical parameters and its function in the development and progression of colorectal carcinoma still remain to b...

journal_title：Biochemical and biophysical research communications

authors： Liu J,Duan Y,Cheng X,Chen X,Xie W,Long H,Lin Z,Zhu B

更新日期：2011-04-08 00:00:00

abstract：:Peptidyl fluoromethyl ketones, improved reagents for inactivating cysteinyl and serine proteinases, have provided unexpected results when applied to intact trypanosomes. A lethal effect was observed but limited to the infectious phase of the parasitic growth cycle. Since the inhibitors are known only to act on proteas...

journal_title：Biochemical and biophysical research communications

authors： Ashall F,Angliker H,Shaw E

更新日期：1990-07-31 00:00:00

abstract：:We recently developed the cellular model MVLN-15 in which estrogenic action can be detected by bioluminescence. Using this cellular model, we characterized the inhibitory effect of retinoic acid on the estrogen-dependent induction of luciferase transcription. We present evidence that i) the inhibitory effect of retino...

journal_title：Biochemical and biophysical research communications

authors： Demirpence E,Pons M,Balaguer P,Gagne D

更新日期：1992-02-28 00:00:00

abstract：:Chronic infection with hepatitis B virus (HBV) is associated with the majority of cases of hepatocellular carcinoma (HCC) in China. Despite this, there is no effective method for the early detection of HBV-induced liver cancer. Aberrant fucosylation is known to occur during the development of HCC. We, therefore, devel...

journal_title：Biochemical and biophysical research communications

authors： Liao J,Zhang R,Qian H,Cao L,Zhang Y,Xu W,Li J,Wu M,Yin Z

更新日期：2012-04-06 00:00:00

abstract：:Necrotic cells are known to develop characteristic membrane blebs. We measured protein concentration within necrotic blebs and found that it can be reduced by as much as twenty-fold compared to the main cell body (CB). These results raise two questions: 1. Why do proteins vacate the bleb? 2. How can osmotic equilibriu...

journal_title：Biochemical and biophysical research communications

authors： Rana PS,Mudrak NJ,Lopez R,Lynn M,Kershner L,Model MA

更新日期：2017-10-21 00:00:00

abstract：:Neu2 mRNA from the mouse thymus, as we have reported [K. Kotani, A. Kuroiwa, T. Saito, Y. Matsuda, T. Koda, S. Kijimoto-Ochiai, Cloning, chromosomal mapping, and characteristic 5'-UTR sequence of murine cytosolic sialidase, Biochem. Biophys. Res. Commun. 286 (2001) 250-258], has a novel sequence at the 5' terminus tha...

journal_title：Biochemical and biophysical research communications

authors： Koda T,Kijimoto-Ochiai S,Uemura S,Inokuchi J

更新日期：2009-10-02 00:00:00

abstract：:The late endosomal marker Rab7 has been long believed to be absent from the phagosome containing Mycobacterium tuberculosis (M.tb) in macrophage, but the detail kinetics remains elusive. Here, we found that Rab7 is transiently recruited to and subsequently released from M.tb phagosomes. For further understanding of th...

journal_title：Biochemical and biophysical research communications

authors： Seto S,Matsumoto S,Ohta I,Tsujimura K,Koide Y

更新日期：2009-09-18 00:00:00

abstract：:To investigate the role of activation of c-Jun NH2-terminal kinase 1 (JNK1) in mediating cisplatin-induced apoptosis and the possibility of induction of JNK activity in triggering relation to DNA damage and drug resistance. We investigated the difference of cisplatin-induced activation of JNK pathway and H2O2 alterati...

journal_title：Biochemical and biophysical research communications

authors： Li F,Meng L,Zhou J,Xing H,Wang S,Xu G,Zhu H,Wang B,Chen G,Lu YP,Ma D

更新日期：2005-10-07 00:00:00

abstract：:MicroRNAs (miRNAs) play critical roles in the proliferation of bovine preadipocytes. miR-2400 is a novel and unique miRNA from bovines. In the present study, we separated and identified preadipocytes from bovine samples. miR-2400 overexpression increased the rate of preadipocyte proliferation, which was analyzed with ...

journal_title：Biochemical and biophysical research communications

authors： Wei Y,Cui YF,Tong HL,Zhang WW,Yan YQ

更新日期：2016-09-23 00:00:00

abstract：:In the current study, we have examined the role of serotonin in regulating the levels of methyl farnesoate and ecdysteroids in the giant mud crab Scylla serrata and validated that serotonin indeed is a reproductive hormone. Administration of serotonin elevated circulatory levels of methyl farnesoate and ecdysteroids i...

journal_title：Biochemical and biophysical research communications

authors： Girish BP,Swetha CH,Reddy PS

更新日期：2017-09-02 00:00:00