Selection and partial characterization of calcium ionophore (A23187) resistant cells.

abstract：:The effect of silkworm hemolymph on baculovirus-induced insect cell apoptosis was investigated. The addition of silkworm hemolymph into the culture medium either before or during the baculovirus infection increased the host cell longevity; however, its addition after the infection was less effective. This can be expla...

journal_title：Biochemical and biophysical research communications

authors： Rhee WJ,Park TH

更新日期：2000-04-29 00:00:00

abstract：:Glypican 3 (GPC3), a GPI-anchored heparan sulfate proteoglycan, is expressed in the majority of hepatocellular carcinoma (HCC) tissues. Using MRL/lpr mice, we successfully generated a series of anti-GPC3 monoclonal antibodies (mAbs). GPC3 was partially cleaved between Arg358 and Ser359, generating a C-terminal 30-kDa ...

journal_title：Biochemical and biophysical research communications

authors： Nakano K,Orita T,Nezu J,Yoshino T,Ohizumi I,Sugimoto M,Furugaki K,Kinoshita Y,Ishiguro T,Hamakubo T,Kodama T,Aburatani H,Yamada-Okabe H,Tsuchiya M

更新日期：2009-01-09 00:00:00

abstract：:Epigenetic gene silencing by aberrant DNA methylation leads to loss of key cellular pathways in tumorigenesis. DNA methylation-mediated silenced genes in pancreatic cancer were searched for using the methyl-CpG targeted transcriptional activation (MeTA) method, and LHX6 (LIM homeobox 6), a transcription factor involve...

journal_title：Biochemical and biophysical research communications

authors： Abudurexiti Y,Gu Z,Chakma K,Hata T,Motoi F,Unno M,Horii A,Fukushige S

更新日期：2020-06-04 00:00:00

abstract：:Trilobolide and 2,5-di(tert-butyl)-1,4-benzohydroquinone (BHQ) are potent inhibitors of the Ca(2+)-ATPase of skeletal muscle sarcoplasmic reticulum. Desoxytrilobolide and 2,5-di(tert-butyl)-1,4-diacetylphenol (acetyl-BHQ) have much lower potencies than their parent compounds and 2,5-di(tert-butyl)-1,4-benzoquinone (BQ...

journal_title：Biochemical and biophysical research communications

authors： Wictome M,Holub M,East JM,Lee AG

更新日期：1994-03-15 00:00:00

abstract：:Polyclonal antibodies (pAbs) to riboflavin carrier protein (RCP) purified from human amniotic fluid were raised in two rabbits. These pAbs apart from recognizing the parent antigen, reacted significantly with chicken RCP as well. When all the 212 octapeptides overlapping by one amino acid residue and covering the enti...

journal_title：Biochemical and biophysical research communications

authors： Subramanian S,Adiga PR

更新日期：1999-08-27 00:00:00

abstract：:Incubation with protein kinase NII did not result in phosphorylation or inactivation of mouse kidney ornithine decarboxylase. Partially purified ornithine decarboxylase preparations contained a protein kinase activity and stimulated the activity of RNA polymerase I. However, these properties were due to contaminating ...

journal_title：Biochemical and biophysical research communications

authors： Seely JE,Stetler DA,Jacob ST,Pegg AE

更新日期：1984-04-16 00:00:00

abstract：:Transforming growth factor alpha (TGF alpha) induces dose- and time-dependent stimulation of prostacyclin (PGI2) production by cultured human umbilical vein endothelial cells. The lowest stimulatory concentration of TGF alpha was 0.1 ng/ml and the maximal response, a 2.7-fold rise, was obtained with 10 ng/ml. The stim...

journal_title：Biochemical and biophysical research communications

authors： Ristimäki A

更新日期：1989-05-15 00:00:00

abstract：:Cigarette smoke is a major risk factor for lung cancer and respiratory infections. This increased susceptibility may result from cigarette smoke-induced impairment of the immune system. In this study, we evaluated the effect of nicotine on the production of inflammatory mediators by activated macrophages. Pretreatment...

journal_title：Biochemical and biophysical research communications

authors： Sugano N,Shimada K,Ito K,Murai S

更新日期：1998-11-09 00:00:00

abstract：:The recent discovery that mutation Asn21 --> Ile in the human cationic trypsinogen (Tg) is associated with hereditary pancreatitis has brought into focus the functional role of amino acid 21 in mammalian Tgs. In the present paper, the effect of mutations Thr21 --> Asn and Thr21 --> Ile on the Ca(2+) dependence of zymo...

journal_title：Biochemical and biophysical research communications

authors： Sahin-Tóth M,Tóth M

更新日期：2000-08-28 00:00:00

abstract：:We examined the contribution of apoptosis- and oxidative stress-associated genes to apoptosis induction in trophoblast cells of human fetal membrane tissues undergoing apoptosis during in vitro incubation. RT-PCR analyses demonstrated an increased level of HO-1, Mn-SOD, Cox-2, iNOS, TNFalpha, TNFR1, IL-1beta, IL-6, Ba...

journal_title：Biochemical and biophysical research communications

authors： Yuan B,Ohyama K,Bessho T,Toyoda H

更新日期：2006-03-17 00:00:00

abstract：:Serotonin is known to be present in pancreatic β-cells and to play several physiological roles, including insulin secretion, β-cell proliferation, and paracrine inhibition of α-cells. However, the serotonin production of different cell lines and islets has not been compared based on age, sex, and diabetes related cond...

journal_title：Biochemical and biophysical research communications

authors： Kim YG,Moon JH,Kim K,Kim H,Kim J,Jeong JS,Lee J,Kang S,Park JS,Kim H

更新日期：2017-11-25 00:00:00

abstract：:Lecithin-cholesterol acyltransferase (LCAT) is responsible for the formation of cholesterol esters in plasma and is implicated in the removal of excess cholesterol from peripheral tissues. It is generally accepted that the catalytic mechanism of LCAT is similar to that of serine proteases and lipases involving a Ser, ...

journal_title：Biochemical and biophysical research communications

authors： Adimoolam S,Lee YP,Jonas A

更新日期：1998-02-13 00:00:00

abstract：:Integrins alpha(M)beta(2) plays important role on leukocytes, such as adhesion, migration, phagocytosis, and apoptosis. It was hypothesized that homomeric associations of integrin subunits provide a driving force for integrins activation, and simultaneously inducing the formation of integrins clusters. However, experi...

journal_title：Biochemical and biophysical research communications

authors： Fu G,Wang C,Wang GY,Chen YZ,He C,Xu ZZ

更新日期：2006-12-29 00:00:00

abstract：:The Apical Junctional Complex (AJC) encompassing the tight junction (TJ) and adherens junction (AJ) plays a pivotal role in regulating epithelial barrier function and epithelial cell proliferative processes through signaling events that remain poorly characterized. A potential regulator of AJC protein expression is Gl...

journal_title：Biochemical and biophysical research communications

authors： Severson EA,Kwon M,Hilgarth RS,Parkos CA,Nusrat A

更新日期：2010-07-02 00:00:00

abstract：:cDNA clones from Nicotiana plumbaginifolia have been isolated by hybridization to a yeast H+-ATPase gene. The largest one encodes a polypeptide (PMA2) of 956 amino acid residues which exhibits a homology of 73% with a limited protein sequence obtained from purified oat plasma membrane H+-ATPase (Schaller and Sussman, ...

journal_title：Biochemical and biophysical research communications

authors： Boutry M,Michelet B,Goffeau A

更新日期：1989-07-31 00:00:00

abstract：:Alzheimer's disease (AD) is one of the most common neurodegenerative diseases leading to dementia. Although cytotoxicity of amyloid β peptides has been intensively studied within pathophysiology of AD, the physiological function of amyloid precursor protein (APP) still remains unclarified. We have shown previously tha...

journal_title：Biochemical and biophysical research communications

authors： Kwak YD,Hendrix BJ,Sugaya K

更新日期：2014-05-09 00:00:00

abstract：:The late endosomal marker Rab7 has been long believed to be absent from the phagosome containing Mycobacterium tuberculosis (M.tb) in macrophage, but the detail kinetics remains elusive. Here, we found that Rab7 is transiently recruited to and subsequently released from M.tb phagosomes. For further understanding of th...

journal_title：Biochemical and biophysical research communications

authors： Seto S,Matsumoto S,Ohta I,Tsujimura K,Koide Y

更新日期：2009-09-18 00:00:00

abstract：:In epithelial cells progression through the G1 phase of the cell cycle and preparing the cell for the S phase is regulated by cyclin D1-cdk4. Cells that express the retinoblastoma protein (pRb) are dependent on cyclin D1-cdk4 activity for their proliferation while cells that do not express pRb are not. Overexpression ...

journal_title：Biochemical and biophysical research communications

authors： Kent LL,Hull-Campbell NE,Lau T,Wu JC,Thompson SA,Nori M

更新日期：1999-07-14 00:00:00

abstract：:The effect of 1-bromlalkanes on intracellular glutathione (GSH) was studied in freshly isolated rat hepatocytes. Treatment of cells with bromoalkanes depleted cellular GSH levels without causing cytotoxicity. The extent of GSH depletion was directly proportional to the concentration and increasing chain length of 1-br...

journal_title：Biochemical and biophysical research communications

authors： Khan S,O'Brien PJ

更新日期：1991-08-30 00:00:00

abstract：:The present study demonstrates that human platelets possess a specific L-arginine transport system able to provide adequate amounts of L-arginine for endogenous nitric oxide production. L-arginine uptake takes place through a saturable high affinity carrier-mediated Na(+)-independent process which is significantly inh...

journal_title：Biochemical and biophysical research communications

authors： Vasta V,Meacci E,Farnararo M,Bruni P

更新日期：1995-01-26 00:00:00

abstract：:Ras proteins can be modified at their COOH-terminal cysteine in the motif Cys-Ali-Ali-Xaa by a farnesyl isoprenoid. This modification is essential for membrane association and biological activity of ras proteins. A similar COOH-terminal amino acid sequence, Cys-Xaa-Ali-Xaa, exists in the ras-related GTP-binding protei...

journal_title：Biochemical and biophysical research communications

authors： Didsbury JR,Uhing RJ,Snyderman R

更新日期：1990-09-14 00:00:00

abstract：:Human leucocytes contain two different MU-NANA neuraminidases, which can be distinguished by Concanavalin A binding. The Con A binding form is predominant in lymphocytes (more than 80%) and the non-binding form predominates in granulocytes. The pH optima of both these neuraminidases as well as their subcellular locali...

journal_title：Biochemical and biophysical research communications

authors： Verheijen FW,Janse HC,van Diggelen OP,Bakker HD,Loonen MC,Durand P,Galjaard H

更新日期：1983-12-16 00:00:00

abstract：:Acyl-CoA:cholesterol acyltransferase (ACAT) plays important roles in cellular cholesterol homeostasis. Two ACAT genes exist in mammals. We report here the genomic organization of human ACAT-2 gene and analysis of its promoter activity in various cell lines. The human ACAT-2 gene spans over 18 kb and contains 15 exons....

journal_title：Biochemical and biophysical research communications

authors： Song BL,Qi W,Yang XY,Chang CC,Zhu JQ,Chang TY,Li BL

更新日期：2001-03-30 00:00:00

abstract：:Inducible nitric oxide (iNOS) is closely correlated with chronic inflammation in hepatitis B virus X protein (HBx)-induced hepatocellular carcinoma (HCC). However, the molecular mechanisms through which iNOS contribute to hepatocarcinogenesis remain poorly understood. Therefore, we investigated the role of iNOS in sig...

journal_title：Biochemical and biophysical research communications

authors： Park YH,Shin HJ,Kim SU,Kim JM,Kim JH,Bang DH,Chang KT,Kim BY,Yu DY

更新日期：2013-05-31 00:00:00

abstract：:The kallikrein inhibitor found in Bauhinia bauhinioides seeds (BbKI) differs from classical Kunitz plant inhibitors in the lack of disulfide bridges in its structure [Biochim. Biophys. Acta 1477 (2000) 64-74]. In this study, we examined whether structural properties may be involved in inhibitory specificity and, if so...

journal_title：Biochemical and biophysical research communications

authors： Cagliari CI,De Caroli FP,Nakahata AM,Araújo MS,Nakaie CR,Sampaio MU,Sampaio CA,Oliva ML

更新日期：2003-11-07 00:00:00

abstract：:Reactive oxygen species (ROS) are a continuous hazard in eukaroytic cells by their ability to cause damage to biomolecules, in particular to DNA. Previous data indicated that the cytosolic serine peptidase tripeptidyl-peptidase II (TPPII) translocates into the nucleus of most tumor cell lines in response to gamma-irra...

journal_title：Biochemical and biophysical research communications

authors： Preta G,de Klark R,Chakraborti S,Glas R

更新日期：2010-08-27 00:00:00

abstract：:Expression of brain-derived neurotrophic factor (BDNF) mRNA is increased in the dorsal root ganglion (DRG) in response to peripheral inflammation. Nerve growth factor (NGF) from inflammatory tissue is thought to induce expression of BDNF. Recently, it was reported that the BDNF gene has eight non-coding exons that are...

journal_title：Biochemical and biophysical research communications

authors： Matsuoka Y,Yokoyama M,Kobayashi H,Omori M,Itano Y,Morita K,Mori H,Nakanishi T

更新日期：2007-10-26 00:00:00

abstract：:The growth of DU-145 human prostate carcinoma cells is reduced to 50% of control by 1 X 10(-6) M to 2 X 10(-6) M selenium and to 2% of control at 10(-4)M selenium. These cells show greater sensitivity to inhibition of growth or DNA synthesis by selenium than human W1-38 and HeLa cells and mouse mammary tumor cells. It...

journal_title：Biochemical and biophysical research communications

authors： Webber MM,Perez-Ripoll EA,James GT

更新日期：1985-07-31 00:00:00

abstract：:AHNAK is a giant protein of approximately 700 kDa identified in human neuroblastomas and skin epithelial cells. Recently, we found that AHNAK knock-out (AHNAK(-/-)) mice have a strong resistance to high-fat diet-induced obesity. In this study, we applied (1)H NMR-based metabolomics with multivariate statistical analys...

journal_title：Biochemical and biophysical research communications

authors： Kim IY,Jung J,Jang M,Ahn YG,Shin JH,Choi JW,Sohn MR,Shin SM,Kang DG,Lee HS,Bae YS,Ryu DH,Seong JK,Hwang GS

更新日期：2010-12-17 00:00:00

abstract：:CD36 expressed on adipocytes is thought to function as a fatty acid transporter (FAT). Here we report that adipocytes can endocytose and lysosomally degrade OxLDL, mainly mediated by CD36. Mouse 3T3-L1 preadipocytes showed marked increase in uptake and degradation of (125)I-OxLDL during their differentiation to adipoc...

journal_title：Biochemical and biophysical research communications

authors： Kuniyasu A,Hayashi S,Nakayama H

更新日期：2002-07-12 00:00:00