Uncovering transcription factor and microRNA risk regulatory pathways associated with osteoarthritis by network analysis.

abstract：:We investigated the capacity of K-252a, an inhibitor of rat brain protein kinase C (PKC), to influence polymorphonuclear neutrophil (PMN) PKC and PMN activation with chemically and structurally dissimilar agonists. K-252a inhibited PMN PKC (IC50 = 0.58 microM), and caused a concentration-dependent (0.1-10 microM) inhi...

journal_title：Biochemical and biophysical research communications

authors： Smith RJ,Justen JM,Sam LM

更新日期：1988-05-16 00:00:00

abstract：:The ATPase activity of F1 isolated from the thermophilic bacterium PS3 is stimulated at 30 degrees C by the anionic detergents cholate or deoxycholate. Maximal activity obtained with these detergents (35 mumol/min X mg) is similar to the activity reported for the optimal temperature, 75 degrees C. The activity is line...

journal_title：Biochemical and biophysical research communications

authors： Norling B

更新日期：1986-05-14 00:00:00

abstract：:The nuclear enzyme poly(ADP-ribosyl) transferase (pADPRT) catalyzes the formation of poly(ADP-ribose) from NAD+. Several nuclear proteins and pADPRT itself are targets for the modification by poly(ADP-ribosyl)ation. It is demonstrated here that poly(ADP-ribose) or pADPRT automodified with poly(ADP-ribose) interacts no...

journal_title：Biochemical and biophysical research communications

authors： Mayer-Kuckuk P,Ullrich O,Ziegler M,Grune T,Schweiger M

更新日期：1999-06-16 00:00:00

abstract：:The conformational dependence of the interresidue interproton distances in peptides, C alpha H ... Ni + 1 H and NiH ... Ni + 1 H, have been used to identify zones of sterically allowed phi, psi space, where both distances are less than 3A and expected to yield nuclear Overhauser effects (NOEs). L-residues in left-hand...

journal_title：Biochemical and biophysical research communications

authors： Ramakrishnan C,Sukumar M,Balaram P

更新日期：1987-12-31 00:00:00

abstract：:PQBP-1 was identified as a binding protein to the polyglutamine tract present in various transcription-related factors and causative genes for neurodegenerative disorders. This novel gene contains at least two functional domains, WW domain and carboxyl-terminal domain (CTD), strictly conserved beyond species. Although...

journal_title：Biochemical and biophysical research communications

authors： Waragai M,Junn E,Kajikawa M,Takeuchi S,Kanazawa I,Shibata M,Mouradian MM,Okazawa H

更新日期：2000-07-05 00:00:00

abstract：:Class II transactivator (CIITA) plays a critical role in controlling major histocompatibility complex (MHC) class II gene expression. In this study, two novel alternatively spliced variants of human interferon (IFN)-γ-inducible CIITA, one missing exon 7 (CIITAΔE7), the other with TAG inserted at exon 4/5 junction (CII...

journal_title：Biochemical and biophysical research communications

authors： Chiu BL,Li CH,Chang CC

更新日期：2013-10-11 00:00:00

abstract：:An inhibitor against the egg beta-D-N-acetylglucosaminidase (GlcNAcase) was purified from the vitelline coat of eggs of the ascidian, Halocynthia roretzi. The purified inhibitor gave a single band with an apparent molecular mass of 70 kDa on SDS-PAGE under non-reducing conditions. The GlcNAcase inhibitor potently inhi...

journal_title：Biochemical and biophysical research communications

authors： Matsuura K,Sawada H,Yokosawa H

更新日期：1995-08-04 00:00:00

abstract：:We have isolated a rabbit metallothionein-I gene from a lambda gt10 library. The coding sequence of this gene is interrupted by two introns occurring at amino acid positions 9 1/3 and 30 1/3. Comparison of the promoter sequence of this gene with the promoters of other metallothionein genes identified a number of oligo...

journal_title：Biochemical and biophysical research communications

authors： Tam YC,Chopra A,Hassan M,Thirion JP

更新日期：1988-05-31 00:00:00

abstract：:The molecular mechanisms by which chronic hypoxia, whether constant (CCH) or intermittent (CIH), alters the heart rhythm are still under debate. Expression level, control, maturational profile and intercoordination of 54 genes encoding heart rhythm determinants (HRDs) were analyzed in 36 mice subjected for 1, 2 or 4 w...

journal_title：Biochemical and biophysical research communications

authors： Iacobas DA,Iacobas S,Haddad GG

更新日期：2010-01-22 00:00:00

abstract：:The calmodulin-like domain of calpain is important for the association of the calpain large and small subunits. We expressed the calmodulin-like domains of the large subunits of rabbit mu- and m-calpains and their small subunits in E. coli and purified them to homogeneity. Unlike the full-length subunits, the calmodul...

journal_title：Biochemical and biophysical research communications

authors： Suo S,Koike H,Sorimachi H,Ishiura S,Suzuki K

更新日期：1999-04-02 00:00:00

abstract：:Carp acclimated to 10 degrees C gave 69k, 66k, and 62kDa light meromyosin (LMM) fragments in SDS-PAGE, while fish acclimated to 30 degrees C gave 74k, 69k, 66k, and 62kDa fragments. The microsequence analysis revealed that the 69k and 66kDa components from the 10 degrees C-acclimated carp contained an N-terminal amino...

journal_title：Biochemical and biophysical research communications

authors： Watabe S,Imai J,Nakaya M,Hirayama Y,Okamoto Y,Masaki H,Uozumi T,Hirono I,Aoki T

更新日期：1995-03-08 00:00:00

abstract：:The effects of substance P on cultured rabbit osteoclasts were investigated. The intracellular Ca(2+) concentration ([Ca(2+)](i)) which was monitored by the microfluorometric technique using fura-2, in osteoclasts elevated by the addition of substance P (0.3-5 microM). The EC(50) value of substance P was about 200 nM....

journal_title：Biochemical and biophysical research communications

authors： Mori T,Ogata T,Okumura H,Shibata T,Nakamura Y,Kataoka K

更新日期：1999-08-27 00:00:00

abstract：:The ras family of oncogenes, as well as the transplasma membrane electron transport system, have been implicated in cellular growth control. Here we present evidence that the Ha-ras oncoprotein increases the activity of the transplasma membrane electron transport system in the mouse embryonic fibroblast cell line C3H1...

journal_title：Biochemical and biophysical research communications

authors： Crowe RA,Taparowsky EJ,Crane FL

更新日期：1993-10-29 00:00:00

abstract：:Poly(ADP-ribose) polymerase (ADPRP) is a nuclear enzyme that transfers ADP-ribose from NAD+ to diverse nuclear proteins. Previously, the function of ADPRP was considered to relate exclusively to its catalytic activity. However, recent experiments have shown that ADPRP is actually an abundant DNA-binding protein, and t...

journal_title：Biochemical and biophysical research communications

authors： Nobori T,Yamanaka H,Carson DA

更新日期：1989-09-15 00:00:00

abstract：:p73 is one of the p53 family members which are transcription factors involved in the regulation of cell proliferation, apoptosis, and differentiation. In this study, we cloned the p73 cDNA from zebrafish ovary RNA. The consensus open reading frame (1923bp) encodes a polypeptide of 640 amino acids which shares 70-95% i...

journal_title：Biochemical and biophysical research communications

authors： Pan H,Dung HN,Hsu HM,Hsiao KM,Chen LY

更新日期：2003-07-25 00:00:00

abstract：:We infected a mixed culture of primary rat astrocytes and microglia with a replication-defective adenovirus carrying the rat glia maturation factor (GMF) cDNA. Affymetrix microarray analysis showed a big increase in the expression of several major histocompatibility complex (MHC) class II proteins along with interleuk...

journal_title：Biochemical and biophysical research communications

authors： Zaheer A,Mathur SN,Lim R

更新日期：2002-06-07 00:00:00

abstract：:Chondrocyte- or chondrosarcoma cell line (HCS)-specific DNA fragments were obtained using differential display-PCR. Nucleotide sequences of 32 species derived from HCS cells were determined. One of the sequence tags (tag no. 24) corresponded to the nucleotide sequence of connective tissue growth factor (CTGF). Norther...

journal_title：Biochemical and biophysical research communications

authors： Nakanishi T,Kimura Y,Tamura T,Ichikawa H,Yamaai Y,Sugimoto T,Takigawa M

更新日期：1997-05-08 00:00:00

abstract：:In a radioassay for Vasoactive Intestinal Peptide (VIP)-binding, eight out of 33 plasma samples from healthy human subjects exhibited specific binding ranging from 2.6% to 46.7% of total [125 I]VIP. This binding was competitively displaced by unlabeled VIP. The structurally homologous peptides, Peptide Histidine Isole...

journal_title：Biochemical and biophysical research communications

authors： Paul S,Heinz-Erian P,Said SI

更新日期：1985-07-16 00:00:00

abstract：:Efficiency of the induction protocol is crucial for the generation of insulin-producing cells (IPCs) from human dental pulp stem cells (hDPSCs). Here, we established the integrative induction protocol by merging genetic manipulation technique with our previous published 3-step induction protocol aiming to enhance the ...

journal_title：Biochemical and biophysical research communications

authors： Sawangmake C,Rodprasert W,Osathanon T,Pavasant P

更新日期：2020-09-10 00:00:00

abstract：:The bacteriophage T7 binary expression system is widely used in vitro for high level selective expression of cloned genes but its application to in vivo models has not yet been investigated. In the present work, we show that coinjection into fertilized zebrafish eggs of pE1T7R, an expression plasmid bearing the T7 RNA...

journal_title：Biochemical and biophysical research communications

authors： Verri T,Argenton F,Tomanin R,Scarpa M,Storelli C,Costa R,Colombo L,Bortolussi M

更新日期：1997-08-28 00:00:00

abstract：:Matrix vesicles (MVs) are cell-derived membranous entities crucial for mineral formation in the extracellular matrix. One of the dominant groups of constitutive proteins present in MVs, recognised as regulators of mineralization in norm and pathology, are annexins. In this report, besides the annexins already describe...

journal_title：Biochemical and biophysical research communications

authors： Cmoch A,Strzelecka-Kiliszek A,Palczewska M,Groves P,Pikula S

更新日期：2011-09-09 00:00:00

abstract：:The integrity and the surface charge distribution of native low density lipoprotein (LDL) are prerequisites of its binding to the LDL receptor. Oxidation is one of the most important physiological effects resulting in an altered structure and metabolism of LDL. To reveal forces responsible for maintaining the intact s...

journal_title：Biochemical and biophysical research communications

authors： Lakatos S,Fürész J,Pállinger E,Rischák K,Schweitzer K,Szollár L

更新日期：1995-11-02 00:00:00

abstract：:Uromodulin (Tamm-Horsfall protein), the most abundant constituent of human urine, is synthesized exclusively in the kidney tubular epithelium and its amino acid sequence suggests a capacity for cell adhesion. We investigated adhesion between human uromodulin and neutrophils by allowing uromodulin, immobilized on micro...

journal_title：Biochemical and biophysical research communications

authors： Toma G,Bates JM Jr,Kumar S

更新日期：1994-04-15 00:00:00

abstract：:Contractile properties differ between skeletal, cardiac and smooth muscles as well as between various skeletal muscle fiber types. This functional diversity is thought to be mainly related to different speeds of myosin head pulling cycles, with the molecular mechanism of force generation being essentially the same. In...

journal_title：Biochemical and biophysical research communications

authors： Galler S,Andruchov O,Stephenson GM,Stephenson DG

更新日期：2009-07-17 00:00:00

abstract：:At 10 microM, 1-0-oleoyl-, 1-0-palmitoyl-, and 1-0-myristoyl-2-0-acetyl-glycerol weakly stimulated neutrophils to release lysozyme, an enzyme in secondary granules, but had no such effect on the release of a primary granule enzyme, beta-glucuronidase. The glycerides (1-10 microM) had a second effect on both granule po...

journal_title：Biochemical and biophysical research communications

authors： O'Flaherty JT,Schmitt JD,McCall CE,Wykle RL

更新日期：1984-08-30 00:00:00

abstract：:This study describes the purification and characterization of an aminopeptidase from human cerebrospinal fluid capable of degrading delta-sleep-inducing-peptide (DSIP). The enzyme has an apparent molecular weight of approximately 80,000 dalton. It is sensitive towards amastatin, bestatin and EDTA and is optimally acti...

journal_title：Biochemical and biophysical research communications

authors： Nyberg F,Thörnwall M,Hetta J

更新日期：1990-03-30 00:00:00

abstract：:Porin or VDAC, the pore forming protein in the outer mitochondrial membrane, was identified as a binding site for microtubule-associated protein 2 (MAP2). By affinity chromatography using covalently bound MAP2, two proteins were selectively purified with molecular weights 63 and 70 KD. The 63 KD protein was identified...

journal_title：Biochemical and biophysical research communications

authors： Lindén M,Karlsson G

更新日期：1996-01-26 00:00:00

abstract：:Regarding the molecular mechanism of dynamin in receptor-mediated endocytosis, GTPase activity of dynamin has been thought to have a critical role in endocytic vesicle internalization. However, a recent report suggested that GTP-binding to dynamin itself activates the dynamin to recruit molecular machinery necessary f...

journal_title：Biochemical and biophysical research communications

authors： Jeong MJ,Yoo J,Lee SS,Lee KI,Cho A,Kwon BM,Moon MJ,Park YM,Han MY

更新日期：2001-04-27 00:00:00

abstract：:Human follitropin receptor (hFSHR) cDNA was inserted into the genome of Autographa californica nuclear polyhedrosis virus under the control of the polyhedrin promoter. Spodoptera frugiperda (Sf9) insect cells expressed the hFSHR at the membrane level, 2-3 days after infection. Ligand binding analysis demonstrated satu...

journal_title：Biochemical and biophysical research communications

authors： Christophe S,Robert P,Maugain S,Bellet D,Koman A,Bidart JM

更新日期：1993-10-15 00:00:00

abstract：:An internal RNA standard proved less suitable in bacterial gene expression experiments. We therefore developed a method for simultaneous RNA and gDNA (genomic DNA) isolation from in vitro and in vivo samples containing staphylococci and combined it with quantitative PCR. The reliability of gDNA for bacterial quantific...

journal_title：Biochemical and biophysical research communications

authors： Vandecasteele SJ,Peetermans WE,Merckx R,Van Ranst M,Van Eldere J

更新日期：2002-03-01 00:00:00