Abstract:
:Maintaining tissue homeostasis is a critical process during infection and inflammation. Tissues with a high intrinsic turnover, such as the intestinal epithelium, must launch a rapid response to infections while simultaneously coordinating cell proliferation and differentiation decisions. In this study, we searched for genes required for regeneration of the Drosophila intestine, and thereby affecting overall organism survival after infection with pathogenic bacteria. We found that Dpp/Gbb (BMP) signaling is essential for normal midgut regeneration, and that infection induces the BMP signaling ligands Dpp and Gbb. We demonstrate that Dpp is induced in visceral muscle and required for signaling activation. Subsequently, Gbb is induced in enterocytes after oral infection. Loss-of Dpp signaling in ISCs and transient committed progenitors called enteroblasts (EBs), or in EBs alone, led to a blockage in EC differentiation or maturation. Furthermore, our data show that down-regulation of Dpp signaling in the precursor cells including EBs also resulted in an increased number of abnormally small Pdm1-positive cells, suggesting a role of Dpp/Gbb signaling in EC growth. In addition, we show that Dpp/Gbb signaling acted downstream or in parallel to the Notch pathway to promote EC differentiation and growth. Our results suggest that Dpp/BMP signaling plays an important role in EBs to maintain tissue integrity and homeostasis during pathogenic infections.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Zhou J,Florescu S,Boettcher AL,Luo L,Dutta D,Kerr G,Cai Y,Edgar BA,Boutros Mdoi
10.1016/j.ydbio.2014.12.017subject
Has Abstractpub_date
2015-03-15 00:00:00pages
189-203issue
2eissn
0012-1606issn
1095-564Xpii
S0012-1606(14)00647-2journal_volume
399pub_type
杂志文章abstract::Mammalian eggs remain arrested at metaphase of the second meiotic division (metII) for an indeterminate time before fertilization. During this period, which can last several hours, the continued attachment of sister chromatids is thought to be achieved by inhibition of the protease separase. Separase is known to be in...
journal_title:Developmental biology
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journal_title:Developmental biology
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journal_title:Developmental biology
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journal_title:Developmental biology
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pub_type: 杂志文章,评审
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journal_title:Developmental biology
pub_type: 杂志文章,评审
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journal_title:Developmental biology
pub_type: 杂志文章
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更新日期:1998-05-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.05.541
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2009.12.030
更新日期:2010-03-15 00:00:00
abstract::Pancreatic organogenesis relies on a complex interplay of cell-autonomous and extracellular signals. We demonstrate that the morphogen sonic hedgehog (Shh) is required for pancreatic development in zebrafish. Genetic mutants of Shh and its signaling pathway establish this dependence as specific to endocrine, but not e...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2002.0573
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journal_title:Developmental biology
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journal_title:Developmental biology
pub_type: 杂志文章
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journal_title:Developmental biology
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doi:10.1016/0012-1606(84)90094-0
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abstract::MicroRNA attenuation of protein translation has emerged as an important regulator of mesenchymal cell differentiation into the osteoblast lineage. A compelling question is the extent to which miR biogenesis is obligatory for bone formation. Here we show conditional deletion of the Dicer enzyme in osteoprogenitors by C...
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pub_type: 杂志文章
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