A Mutagenic Primer Assay for Genotyping of the CRHR1 Gene Rare Variant rs1876828 (A/G) in Asians: A Cost-Effective SNP Typing.

abstract：:For many years, fluorescence polarization immunoassay (FPIA) on the TDx analyzer has been used for determination of free phenytoin concentration. Recently Abbott Laboratories decided to discontinue the TDx analyzer and related assays on this analyzer. Free phenytoin assay is also available from Roche Diagnostics for a...

journal_title：Journal of clinical laboratory analysis

authors： Dasgupta A,Davis B,Chow L

更新日期：2013-01-01 00:00:00

abstract：INTRODUCTION:The Mindray CAL 8000 is a cellular analysis line that consists of the BC-6800, an automated hematology analyzer, and the SC-120, an automated slidemaker/stainer. We evaluated the performances of the BC-6800 and the SC-120. METHODS:Four hundred and eight normal and abnormal samples were analyzed. The perfo...

journal_title：Journal of clinical laboratory analysis

authors： Lee HT,Park PW,Seo YH,Kim KH,Seo JY,Jeong JH,Kim MJ,Ahn JY

更新日期：2017-07-01 00:00:00

abstract：BACKGROUND:Modern automated laboratory hematology analyzers allow the measurement of over 30 different hematological parameters useful in the diagnostic and clinical interpretation of patient symptoms. They use different methods to measure the same parameters. Thus, a comparison of complete blood count made by Mindray ...

journal_title：Journal of clinical laboratory analysis

authors： Ciepiela O,Kotuła I,Kierat S,Sieczkowska S,Podsiadłowska A,Jenczelewska A,Księżarczyk K,Demkow U

更新日期：2016-11-01 00:00:00

abstract：BACKGROUND:Circular RNAs (circRNAs) are emerging noncoding RNAs that are involved in many biological processes and diseases. The expression profile of circRNAs in preterm neonates with bronchopulmonary dysplasia (BPD) remains unresolved. METHODS:In BPD infants, peripheral venous blood was drawn and circRNAs were extra...

journal_title：Journal of clinical laboratory analysis

authors： Mao X,Guo Y,Qiu J,Zhao L,Xu J,Yin J,Lu K,Zhang M,Cheng R

更新日期：2020-07-01 00:00:00

abstract：:Polyfunctional hydrophilic microspheres of 125-nm diameter can be produced by copolymerization of acrylic monomers. Purified c-reactive protein (CRP) was covalently bound to these new micropheres, and the conjugate obtained was used as reagent in a microparticle-enhanced nephelometric immunoassay for human CRP. This a...

journal_title：Journal of clinical laboratory analysis

authors： Montagne P,Laroche P,Cuillière ML,Varcin P,Pau B,Duheille J

更新日期：1992-01-01 00:00:00

abstract：:We here describe an ion-exchange high-performance liquid chromatography technique with electrochemical detection for rapid quantification of glutathione, homocysteine, cysteinylglycine, and methionine. The analytical validation of the technique showed within-assay and between-assay coefficients of variation between 3....

journal_title：Journal of clinical laboratory analysis

authors： Houze P,Gamra S,Madelaine I,Bousquet B,Gourmel B

更新日期：2001-01-01 00:00:00

abstract：BACKGROUND:We propose a simple, sensitive, and fast high-performance liquid chromatography ultraviolet detection (HPLC-UV) method for the quantitative determination of bosutinib in human plasma. METHODS:Plasma samples were processed using an Oasis hydrophilic-lipophilic balance extraction cartridge (1 mL, 30 mg). Bosu...

journal_title：Journal of clinical laboratory analysis

authors： Sumimoto T,Nakahara R,Sato Y,Itoh H

更新日期：2018-01-01 00:00:00

abstract：BACKGROUND:Wolfram's syndrome (WFS) is a hereditary (autosomal recessive) neurodegenerative disorder. The clinical features are related to diabetes insipidus, diabetes mellitus, optic atrophy, and deafness (DIDMOAD) with other variable clinical manifestations. Pathogenic variants in the WFS1 gene, encoding wolframin, a...

journal_title：Journal of clinical laboratory analysis

authors： Torkamandi S,Rezaei S,Mirfakhraie R,Bayat S,Piltan S,Gholami M

更新日期：2020-08-01 00:00:00

abstract：BACKGROUND:To determine the metabolic characteristics of patients with colon cancer (CC) and rectal cancer (RC) using gas chromatography-mass spectrometry (GC-MS)-based metabolomics. METHODS:In this study, serum samples were collected from 22 CC patients and 23 RC patients preoperatively and postoperatively and 45 hea...

journal_title：Journal of clinical laboratory analysis

authors： Wu J,Wu M,Wu Q

更新日期：2020-08-01 00:00:00

abstract：BACKGROUND:Cellular and brain metabolism of dopamine can be correlated with a number of neurodegenerative disorders, our study was to explore a simple and efficient method to detect dopamine in real samples. METHODS:A new quantum dots (CdTe QDs) could be prepared using the hydrothermal method, the electrochemical bios...

journal_title：Journal of clinical laboratory analysis

authors： Yu HW,Zhang Z,Jiang JH,Pan HZ,Chang D

更新日期：2018-03-01 00:00:00

abstract：:The genetic basis of infertility has received increasing recognition in recent years, particularly with the advent of assisted reproductive technology. It is now becoming obvious that genetic etiology for infertility is an important cause of disrupted spermatogenesis. Y-chromosome microdeletions and abnormal karyotype...

journal_title：Journal of clinical laboratory analysis

authors： Abid S,Maitra A,Meherji P,Patel Z,Kadam S,Shah J,Shah R,Kulkarni V,Baburao V,Gokral J

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:The current transfusion policy recommended for individuals with serologic weak-D phenotype is based on data derived from European-descent populations. Data referring to the distribution of RH alleles underlying weak-D phenotype among people of mixed origin are yet incomplete, and the applicability of Europea...

journal_title：Journal of clinical laboratory analysis

authors： Dezan MR,Oliveira VB,Gomes ÇN,Luz F,Gallucci AJ,Bonifácio SL,Alencar CS,Sabino EC,Pereira AC,Krieger JE,Rocha V,Mendrone-Junior A,Dinardo CL

更新日期：2018-11-01 00:00:00

abstract：BACKGROUND:The GABA-A receptor signaling pathway regulates proliferation, differentiation, apoptosis, and responses to overt DNA damage during embryonic development. METHODS:To analyze the gene expression after intracytoplasmic sperm injection (ICSI) and in in vivo mouse embryos, the global pattern of gene expression ...

journal_title：Journal of clinical laboratory analysis

authors： Zhang Z,Wang T,Huang J,Huang Y,Zhang Q

更新日期：2020-09-20 00:00:00

abstract：:Graphical representation of laboratory data speeds up recognition of abnormalities. The Chernoff face is an excellent tool to represent patient data; the human eye and mind are exquisitely sensitive to subtle changes in facial appearances and expression. One of the best uses of the faces is in illustrating trends in t...

journal_title：Journal of clinical laboratory analysis

authors： Lott JA,Durbridge TC

更新日期：1990-01-01 00:00:00

abstract：:The direct agglutination test (DAT) and the enzyme-linked immunosorbent assay (ELISA) were used for serodiagnosis of parasitologically confirmed Indian visceral leishmaniasis (VL) cases. All the sera of VL cases were positive by both the methods. DAT titres of VL cases were greater than or equal to 1:3,200, and ELISA ...

journal_title：Journal of clinical laboratory analysis

authors： Pal A,Mukerji K,Basu D,Naskar K,Mallick KK,Ghosh DK

更新日期：1991-01-01 00:00:00

abstract：:Clinicians recognize and compensate for limitations in estimating the glomerular filtration rate (GFR) using serum creatinine (sCr) measurements by the use of timed collections and mathematical manipulations of sCr. These limitations stem from that fact that sCr is affected by nonrenal influences, including muscle mas...

journal_title：Journal of clinical laboratory analysis

authors： Massey D

更新日期：2004-01-01 00:00:00

abstract：BACKGROUND:Identification of biomarkers for acute myeloid leukemia (AML) is important for treating this malignancy. Recent studies have reported that microRNAs (miRNAs) are stably detectable in the blood/plasma and can be used as biomarkers for various types of cancer including AML. The aim of this study was to analyze...

journal_title：Journal of clinical laboratory analysis

authors： Yu G,Yin Z,He H,Zheng Z,Chai Y,Xuan L,Lin R,Wang Q,Li J,Xu D

更新日期：2020-03-01 00:00:00

abstract：BACKGROUND:Alport syndrome (ATS) is a hereditary nephritis with hereditary and clinical heterogeneity; the early clinical symptoms are atypical, which can easily lead to misdiagnosis. The proband, a 6-year-old girl, was found to have microscopic hematuria, proteinuria, and visual impairment at about 5 years old; the re...

journal_title：Journal of clinical laboratory analysis

authors： Zhu Q,Zhou C,Wang J

更新日期：2020-09-06 00:00:00

abstract：BACKGROUNDS:β-Thalassemia is one of the most prevalent worldwide autosomal recessive disorders. It presents a great molecular heterogeneity resulting from more than 200 causative mutations in the β-globin gene. In Tunisia, β-thalassemia represents the most prevalent monogenic hemoglobin disorder with 2.21% of carriers....

journal_title：Journal of clinical laboratory analysis

authors： Sahli CA,Ben Salem I,Jouini L,Laouini N,Dabboubi R,Hadj Fredj S,Siala H,Othmeni R,Dakhlaoui B,Fattoum S,Bibi A,Messaoud T

更新日期：2016-09-01 00:00:00

abstract：BACKGROUND:The human epididymis protein 4 (HE4) may have high specificity in the detection of malignant diseases, making the development of an immunoassay for HE4 essential. METHODS:In our study, a fusion gene was constructed encoded with the HE4 protein. This protein was then produced in the bacterial cells (Escheric...

journal_title：Journal of clinical laboratory analysis

authors： Zhou L,Lv Z,Shao J,Xu Y,Luo X,Zhang Y,Hu Y,Zhang W,Luo S,Fang J,Wang Y,Duan C,Huang R

更新日期：2016-09-01 00:00:00

abstract：:Epithelial cells are typically connected through different types of cell junctions that are localized from the apical membrane to the basal surface. In this way, epithelium cells form the first barrier against pathogenic microorganisms and prevent their entry into internal organs and the circulatory system. Recent stu...

journal_title：Journal of clinical laboratory analysis

authors： Zheng M,Sun S,Zhou J,Liu M

更新日期：2020-10-17 00:00:00

abstract：:We described an automated enzymatic assay for conjugated bilirubin (Bc) in serum using the Iatro D-Bil kit, with bilirubin oxidase (EC 1.3.3.5 BOD) from Myrothecium species. The specificity of the enzyme in the Iatro D-Bil kit was examined by analyzing unconjugated bilirubin (Bu), delta bilirubin (Bdelta), and Bc with...

journal_title：Journal of clinical laboratory analysis

authors： Kimura S,Iyama S,Yamaguchi Y,Hayashi S,Yanagihara T

更新日期：1999-01-01 00:00:00

abstract：OBJECTIVES:To evaluate the associations of urinary markers (eg albumin), glomerular (eg transferrin [TRF], immunoglobulin G [IgG]), and tubular (eg α1-microglobulin [α1-MG], β2-microglobulin [β2-MG]) markers with the development of diabetic kidney disease (DKD) in type 2 diabetes patients, as assessed by estimated glom...

journal_title：Journal of clinical laboratory analysis

authors： Jiang X,Zhang Q,Wang HB,Cui XF,Liu R

更新日期：2018-01-01 00:00:00

abstract：:α-Feto protein (AFP) is the widely used tumor marker in the diagnosis of hepatocellular carcinoma (HCC). The aim of this study was to assess the diagnostic and prognostic validity of a novel marker, serum Glypican-3 (GPC3) and to compare AFP in patients with HCC. One hundred and twenty-eight patients (75 patients with...

journal_title：Journal of clinical laboratory analysis

authors： Ozkan H,Erdal H,Koçak E,Tutkak H,Karaeren Z,Yakut M,Köklü S

更新日期：2011-01-01 00:00:00

abstract：BACKGROUND:Selection and verification of blood collection tubes is an important preanalytical issue in clinical laboratories. Today, gel tubes are commonly used with many advantages, although they are known to cause interference in immunoassay methods. In this study, we aimed to compare SSTs of two different suppliers ...

journal_title：Journal of clinical laboratory analysis

authors： Ercan M,Fırat Oğuz E,Akbulut ED,Yilmaz M,Turhan T

更新日期：2018-07-01 00:00:00

abstract：BACKGROUND:Spinal tuberculosis is the most common form of musculoskeletal tuberculosis. The expression of matrix metalloproteinase-1 (MMP-1) is increased in cells with Mycobacterium tuberculosis infection. MMP-1 plays a curial role in extracellular matrix degradation during the progression of tuberculosis. Although the...

journal_title：Journal of clinical laboratory analysis

authors： Zhou Y,Gao Q,He D,Deng A,Huang R,Li Y,Tan C,Guo C,Guo Q,Wang L,Yang G,Zhang H

更新日期：2017-11-01 00:00:00

abstract：:It is believed that boundary compositions of matrix proteins might play a role in stone formation; however, few proteomic studies concerning matrix proteins in urinary stones have been conducted. In this study, we extracted low molecular weight proteins from calcium oxalate stones and measured their characteristic pat...

journal_title：Journal of clinical laboratory analysis

authors： Chen WC,Lai CC,Tsai Y,Lin WY,Tsai FJ

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:N-terminal pro-Brain natriuretic peptide (NT-proBNP) is an important biomarker to indicate cardiac function, but its significance in cardiac surgery is still unclear. OBJECTIVE:To explore clinical significance of serum NT-proBNP and their dynamic characteristics in patients with cardiac valve replacement (C...

journal_title：Journal of clinical laboratory analysis

authors： Cai B,Wang L,Liu J,Shi Y,Guo Y

更新日期：2011-01-01 00:00:00

abstract：BACKGROUND:Chronic hepatitis B (CHB) is one of the major infectious diseases in which CD4+ T helper (Th) cells play a crucial role. Th9 cells are a distinct subset of CD4+ Th cells with important physiological functions. OBJECTIVE:The study aimed to assess the potential involvement of Th9 cells in the inadequate immun...

journal_title：Journal of clinical laboratory analysis

authors： Cui M,Lv Y,Lu J,Zhang W,Duan Y,Huang Y,Yang L,Li M,Liu W,Liu D,Yan H

更新日期：2018-02-01 00:00:00

abstract：:Previous studies have shown elevated serum levels of interleukin 6 (IL-6) and C-reactive protein (CRP) in patients with pancreatic cancer (PC). The aim of this study was to assess the diagnostic usefulness of pretreatment serum levels of IL-6 and CRP to differentiate between PC and chronic pancreatitis (CP) patients. ...

journal_title：Journal of clinical laboratory analysis

authors： Mroczko B,Groblewska M,Gryko M,Kedra B,Szmitkowski M

更新日期：2010-01-01 00:00:00