Abstract:
:We here describe an ion-exchange high-performance liquid chromatography technique with electrochemical detection for rapid quantification of glutathione, homocysteine, cysteinylglycine, and methionine. The analytical validation of the technique showed within-assay and between-assay coefficients of variation between 3.1 and 4.3%, and 3.7 and 8.6%, respectively. Percentages of recovery for overload and dilution tests were between 87 and 120%. Detection limits were 1 micromol/L for methionine and 0.5 micromol/L for other compounds. There was no interference with any physiological and pharmacological substances possessing a thiol function. Aminothiol concentrations determined in 100 control subjects (50 women and 50 men) showed no age- or sex-rated differences for except for homocysteine which was increased (+ 28%) in oldest subjects of both sexes. In 60 patients at risk (30 with chronic renal failure, 30 with diabetes), homocysteine concentration was significantly increased. No variation in other aminothiols was observed in diabetic subjects. Methionine was decreased and cysteinylglycine was increased in patients with chronic renal failure. The present technique-rapid, easy to use, and reliable-appears suitable for routine application in the exploration of aminothiol metabolic pathways including mechanisms of hyperhomocysteinemia.
journal_name
J Clin Lab Analjournal_title
Journal of clinical laboratory analysisauthors
Houze P,Gamra S,Madelaine I,Bousquet B,Gourmel Bdoi
10.1002/jcla.1018subject
Has Abstractpub_date
2001-01-01 00:00:00pages
144-53issue
3eissn
0887-8013issn
1098-2825pii
10.1002/jcla.1018journal_volume
15pub_type
杂志文章abstract::We evaluated the Technicon DPA-1 immunoassay analyzer on its analytical characteristics. Therefore we studied three assays: albumin in cerebrospinal fluid and IgG and transferrin in serum. When tested with the Cusum test for linearity albumin, IgG, and transferrin measurements showed no deviation from linearity. Close...
journal_title:Journal of clinical laboratory analysis
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journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
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更新日期:1994-01-01 00:00:00
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journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
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journal_title:Journal of clinical laboratory analysis
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journal_title:Journal of clinical laboratory analysis
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journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
doi:10.1002/jcla.1860040503
更新日期:1990-01-01 00:00:00
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journal_title:Journal of clinical laboratory analysis
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doi:10.1002/jcla.23057
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journal_title:Journal of clinical laboratory analysis
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doi:10.1002/jcla.22842
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journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章,评审
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journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
doi:10.1002/(sici)1098-2825(1998)12:6<378::aid-jcla8>3
更新日期:1998-01-01 00:00:00
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journal_title:Journal of clinical laboratory analysis
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doi:10.1002/(SICI)1098-2825(1996)10:5<302::AID-JCLA11>
更新日期:1996-01-01 00:00:00
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journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
doi:10.1002/jcla.20080
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journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
doi:10.1002/jcla.21710
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abstract::T-lymphocyte function was investigated in a group of eight patients with polycythemia vera (PV) and 13 normals. Responsiveness of T-cells to stimulation by mitogen was significantly impaired in the PV patients (p = .02). Analysis of immune cell subpopulation composition by assessment of cell surface phenotype did not ...
journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
doi:10.1002/jcla.1860030204
更新日期:1989-01-01 00:00:00
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journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
doi:10.1002/jcla.22841
更新日期:2019-05-01 00:00:00