Histocompatibility screening by molecular techniques: use of polymerase chain reaction products and heteroduplex formation.

abstract：BACKGROUND:This study aimed to investigate the plasma long non-coding RNA (lncRNA) expression profile in knee osteoarthritis (KOA) patients and the value of candidate lncRNAs for predicting KOA risk. METHODS:Plasma was obtained for RNA sequencing (RNA-seq) in eight KOA patients and eight healthy controls (Ctrls). Ten ...

journal_title：Journal of clinical laboratory analysis

authors： Liu X,Li M,Zhang B,Zhang N,Feng Q

更新日期：2020-09-01 00:00:00

abstract：:The objectives of this research were to establish an automatic analysis method for the determination of serum argininosuccinate lyase (ASL) and to investigate the value of serum ASL test in the diagnosis of various liver disorders. According to the chemical reaction catalyzed by ASL, an enzyme-coupled reaction system ...

journal_title：Journal of clinical laboratory analysis

authors： Feng JF,Chen TM,Wen YA,Wang J,Tu ZG

更新日期：2008-01-01 00:00:00

abstract：:We investigated the relationship between the urokinase type plasminogen activator receptor (uPAR) in sera and tissues of patients with cervical cancer and the clinical and pathological features of the cancer. Immunohistochemistry (SABC method) was used to detect uPAR expression in cervical cancer and normal tissues; E...

journal_title：Journal of clinical laboratory analysis

authors： Jing J,Zheng S,Han C,Du L,Guo Y,Wang P

更新日期：2012-01-01 00:00:00

abstract：:Clinicians recognize and compensate for limitations in estimating the glomerular filtration rate (GFR) using serum creatinine (sCr) measurements by the use of timed collections and mathematical manipulations of sCr. These limitations stem from that fact that sCr is affected by nonrenal influences, including muscle mas...

journal_title：Journal of clinical laboratory analysis

authors： Massey D

更新日期：2004-01-01 00:00:00

abstract：BACKGROUND:Osteoarthritis (OA) is a popular arthrosis featured as pain, limited joint activity, and deformity. Cyclooxygenase-2 (COX-2) has been reported to be up-regulated in arthritic tissues and is integral to the progression of osteoarthritis (OA). Previous studies showed the COX-2 promoter G-765C polymorphism coul...

journal_title：Journal of clinical laboratory analysis

authors： Huang W,Deng C,Tian F,Gao W,Ding Z,Rao X,Yin Z

更新日期：2018-07-01 00:00:00

abstract：BACKGROUND:Circular RNAs (circRNAs) are emerging noncoding RNAs that are involved in many biological processes and diseases. The expression profile of circRNAs in preterm neonates with bronchopulmonary dysplasia (BPD) remains unresolved. METHODS:In BPD infants, peripheral venous blood was drawn and circRNAs were extra...

journal_title：Journal of clinical laboratory analysis

authors： Mao X,Guo Y,Qiu J,Zhao L,Xu J,Yin J,Lu K,Zhang M,Cheng R

更新日期：2020-07-01 00:00:00

abstract：:For many years, fluorescence polarization immunoassay (FPIA) on the TDx analyzer has been used for determination of free phenytoin concentration. Recently Abbott Laboratories decided to discontinue the TDx analyzer and related assays on this analyzer. Free phenytoin assay is also available from Roche Diagnostics for a...

journal_title：Journal of clinical laboratory analysis

authors： Dasgupta A,Davis B,Chow L

更新日期：2013-01-01 00:00:00

abstract：BACKGROUND:CD4(+) cell count, the common HIV infection screening test, is costly and unable to differentiate HIV monoinfection from its concurrent infection with hepatitis B or C virus. We aimed to ascertain diagnostic value of serum adenosine deaminase (ADA) activity as a useful tool to differentiate HIV mono- and co-...

journal_title：Journal of clinical laboratory analysis

authors： Abdi M,Rahbari R,Khatooni Z,Naseri N,Najafi A,Khodadadi I

更新日期：2016-05-01 00:00:00

abstract：:This study was designed to measure the correlation between left ventricular (LV) remodeling 3 months after successful reperfusion therapy, and the levels of serum N-terminal pro-brain natriuretic peptide (NT-proBNP) and high-sensitivity C-reactive protein (hs-CRP) at 3 days after acute myocardial infarction (AMI), and...

journal_title：Journal of clinical laboratory analysis

authors： Xiaozhou H,Jie Z,Li Z,Liyan C

更新日期：2006-01-01 00:00:00

abstract：BACKGROUND:Acute coronary syndrome (ACS) is the leading cause of death in developing and developed countries, yet assessing the risk of its development remains challenging. Several lines of evidence indicate that small, dense low-density lipoproteins (sd-LDL) are associated with increased cardiovascular disease risk. W...

journal_title：Journal of clinical laboratory analysis

authors： Wu B,Yu Z,Tong T,Tong X,Yang Y,Tang Y,Ren H,Liao Y,Liao J

更新日期：2020-03-01 00:00:00

abstract：:We have evaluated a new latex nephelometric test for the quantitation of myoglobin in human serum. The assay consists of incubating the diluted serum sample (20-fold) for 12 min at room temperature with latex particles covalently coated with anti-myoglobin antibodies and then quantifying the change of light-scatter pr...

journal_title：Journal of clinical laboratory analysis

authors： Borque L,Rus A,Ruiz R

更新日期：1991-01-01 00:00:00

abstract：:A sensitive time-resolved fluorimetric immune-complex-transfer immunoassay for antithyroglobulin IgG in serum is described. Antithyroglobulin IgG in test serum was reacted with dinitrophenyl europium ion-labeled thyroglobulin. The complex formed of antithyroglobulin IgG and dinitrophenyl europium ion-labeled thyroglob...

journal_title：Journal of clinical laboratory analysis

authors： Kohno T,Mitsukawa T,Matsukura S,Ishikawa E

更新日期：1990-01-01 00:00:00

abstract：:United States Food and Drug Administration (FDA) in 2010 approved the use of immunosuppressant drug everolimus, which requires therapeutic drug monitoring in whole blood. Taking advantage of structural similarity between sirolimus and everolimus we attempted to rapidly estimate everolimus concentration from apparent s...

journal_title：Journal of clinical laboratory analysis

authors： Dasgupta A,Moreno V,Balark S,Smith A,Sonilal M,Tejpal N,Van Buren CT

更新日期：2011-01-01 00:00:00

abstract：BACKGROUND:The use of dried blood spots (DBS) for the assay of lysosomal enzymes has facilitated the implementation of pilot studies for newborn screening for lysosomal storage disorders in various developed countries. The aim of the study was to determine the influence of ambient temperature during DBS preparation and...

journal_title：Journal of clinical laboratory analysis

authors： Supriya M,De T,Christopher R

更新日期：2018-01-01 00:00:00

abstract：:Polyfunctional hydrophilic microspheres of 125-nm diameter can be produced by copolymerization of acrylic monomers. Purified c-reactive protein (CRP) was covalently bound to these new micropheres, and the conjugate obtained was used as reagent in a microparticle-enhanced nephelometric immunoassay for human CRP. This a...

journal_title：Journal of clinical laboratory analysis

authors： Montagne P,Laroche P,Cuillière ML,Varcin P,Pau B,Duheille J

更新日期：1992-01-01 00:00:00

abstract：BACKGROUND:Modern automated laboratory hematology analyzers allow the measurement of over 30 different hematological parameters useful in the diagnostic and clinical interpretation of patient symptoms. They use different methods to measure the same parameters. Thus, a comparison of complete blood count made by Mindray ...

journal_title：Journal of clinical laboratory analysis

authors： Ciepiela O,Kotuła I,Kierat S,Sieczkowska S,Podsiadłowska A,Jenczelewska A,Księżarczyk K,Demkow U

更新日期：2016-11-01 00:00:00

abstract：:We studied 103 patients seen in our Prostate Cancer Detection Clinic to determine whether a correlation exists between serum prostate-specific antigen (PSA) values and ultrasound-calculated prostate gland volume. Seventy men (68%) had a PSA value less than or equal to 4 ng/ml (our upper limit of normal). The men were ...

journal_title：Journal of clinical laboratory analysis

authors： Babaian RJ,Fritsche HA,Evans RB

更新日期：1990-01-01 00:00:00

abstract：BACKGROUND:Although recent studies have indicated that gut microbiome dysbiosis was significantly associated with the onset of type 2 diabetes mellitus (T2DM), information on the role of blood microbiome in T2DM development is scarce. METHODS:Fifty incident T2DM cases and 100 matched non-T2DM controls were selected fr...

journal_title：Journal of clinical laboratory analysis

authors： Qiu J,Zhou H,Jing Y,Dong C

更新日期：2019-05-01 00:00:00

abstract：OBJECTIVES:Existing evidence suggests that impaired vitamin D metabolism contribute to the development of atherosclerosis. Aortic intima-media thickness (IMT) is an earlier marker than carotid IMT of preclinical atherosclerosis. However, there is a lack of researches on direct investigation of relevance between serum 2...

journal_title：Journal of clinical laboratory analysis

authors： Kalkan GY,Gür M,Koyunsever NY,Şeker T,Gözükara MY,Uçar H,Kaypaklı O,Baykan AO,Akyol S,Türkoğlu C,Elbasan Z,Şahin DY,Çaylı M

更新日期：2015-07-01 00:00:00

abstract：:Several retrospective studies have shown that a high level of microsatellite instability (MSI-H) is an important prognostic factor of a more favorable outcome in stage II and III colorectal cancer (CRC) patients. In this study, three commonly used polymerase chain reaction (PCR)-based MSI analysis techniques were comp...

journal_title：Journal of clinical laboratory analysis

authors： Deschoolmeester V,Baay M,Wuyts W,Van Marck E,Pelckmans P,Lardon F,Vermorken JB

更新日期：2006-01-01 00:00:00

abstract：:We report nine donations with ABO inconsistency in reverse typing caused by partly or entirely missing antibodies. A and B antigens and antibodies were examined by serological blood typing, and ABO deoxyribonucleic acid (DNA) analyses were performed by sequence-specific priming and sequencing. A B101 allele was demons...

journal_title：Journal of clinical laboratory analysis

authors： Deng ZH,Zeng JQ,Yu Q,Su YQ,Liang YL,Lu L,Zhu WG,Yang BC

更新日期：2007-01-01 00:00:00

abstract：BACKGROUND:Kawasaki disease (KD) is an acute, self-limited vasculitis in infants and young children. Interleukin-10 (IL-10) is a potent cytokine that exerts pleiotropic effects on immunoregulation and inflammation. Elevated IL-10 serum levels have been reported in the KD patients. METHODS:In this study, we investigate...

journal_title：Journal of clinical laboratory analysis

authors： Lin YJ,Lan YC,Lai CH,Lin TH,Huang SM,Liao CC,Lin CW,Hung CH,Tien N,Liu X,Chien WK,Chen JH,Tsai FJ

更新日期：2014-11-01 00:00:00

abstract：BACKGROUND:Though recent reports have indicated a higher prevalence of latent tuberculosis infection (LTBI) in laboratory personnel than in other healthcare workers, these studies included only a limited number of laboratory personnel. METHODS:We have thus focused on the laboratory personnel, who had a high level of e...

journal_title：Journal of clinical laboratory analysis

authors： Moon HW,Kim H,Hur M,Yun YM,Lee A

更新日期：2011-11-01 00:00:00

abstract：BACKGROUND:Normal serum alanine aminotransferase (ALT) levels differ with age, gender, and body mass index. Adjusting the upper limits of normal (ULN) for ALT needs further research in different populations. Aim of this work was to monitor the effect of successful chronic hepatitis C (CHC) treatment on the ALT levels i...

journal_title：Journal of clinical laboratory analysis

authors： El Kassas M,Alboraie M,Mostafa A,Ezzat R,El Tahan A,Afify S,Sweedy A,Kabbash I,Esmat G

更新日期：2018-03-01 00:00:00

abstract：BACKGROUND:This study aimed to investigate the relationship between HbA1c and RBC aggregation in type 2 diabetes mellitus (T2DM) patients by analysis of data from routine clinical tests and from in vitro experiments. METHODS:A total of 2,111 inpatients with type 2 diabetes were selected and among them, 364 patients (G...

journal_title：Journal of clinical laboratory analysis

authors： Li Q,Li L,Li Y

更新日期：2015-09-01 00:00:00

abstract：BACKGROUND:Enzyme-linked immunosorbent assay (ELISA) has traditionally been used to detect myeloperoxidase (MPO) and proteinase 3 (PR3) antibodies, although it is time-consuming and physically demanding. As a novel and highly effective immunoassay, we compared chemiluminescent immunoassay (CIA) with ELISA to verify the...

journal_title：Journal of clinical laboratory analysis

authors： Hou X,Liu J,Wang T,Zhou J,Cui L

更新日期：2020-10-09 00:00:00

abstract：:Using a specific serum anti-soluble T lymphocytes receptor for sheep erythrocytes (E) and SDS-PAGE, we detected radioactive bands of molecular weight 58,000 in immunoprecipitates of supernatant of heated human lymphocytes (SHL), in the supernatant of PHA stimulated lymphocyte cultures (SLC), normal human serum (NHS), ...

journal_title：Journal of clinical laboratory analysis

authors： Itano EN,Ono MA,Sumigawa M,Longo IM,Moura NC,Mendes NF

更新日期：1991-01-01 00:00:00

abstract：BACKGROUND:Identification of dermatophytes at the species level, relying on macro- and microscopic properties of the colonies is time-consuming, questioned in many circumstances, and requires considerable expertise. In this study, we examined the potency of a new genetic marker, β-tubulin (BT2) gene, for differentiatio...

journal_title：Journal of clinical laboratory analysis

authors： Abastabar M,Mirhendi H,Rezaei-Matehkolaei A,Shidfar MR,Kordbacheh P,Makimura K

更新日期：2014-03-01 00:00:00

abstract：:The proteoglycan decorin inhibits TGF-beta; therefore, it could antagonize progression of fibrotic diseases associated with activation of TGF-beta(1). The effect of decorin transfection in human mesangial cells (HMCs) on the expression of genes related to kidney fibrosis was investigated. HMCs, isolated from glomeruli...

journal_title：Journal of clinical laboratory analysis

authors： Costacurta A,Priante G,D'Angelo A,Chieco-Bianchi L,Cantaro S

更新日期：2002-01-01 00:00:00

abstract：BACKGROUND:This study was aimed to investigate whether measurement of free testosterone and cortisol in saliva is a reliable alternative to their assessment in serum for monitoring physical fitness in professional athletes. METHODS:We studied 25 members of the soccer team Parma F.C., playing in Italian major football ...

journal_title：Journal of clinical laboratory analysis

authors： Lippi G,Dipalo M,Buonocore R,Gnocchi C,Aloe R,Delsignore R

更新日期：2016-09-01 00:00:00