Validation of a free phenytoin assay on Cobas c501 analyzer using calibrators from Cobas Integra free phenytoin assay by comparing its performance with fluorescence polarization immunoassay for free phenytoin on the TDx analyzer.

Abstract:

:For many years, fluorescence polarization immunoassay (FPIA) on the TDx analyzer has been used for determination of free phenytoin concentration. Recently Abbott Laboratories decided to discontinue the TDx analyzer and related assays on this analyzer. Free phenytoin assay is also available from Roche Diagnostics for application on the Cobas Integra analyzer (fluorescence polarization assay) but not on Cobas c510 analyzer. Free phenytoin calibrators from the Cobas Integra free phenytoin assay and the reagents from the KIMSphenytoin assay were used for the determination of free phenytoin on the Cobas c501 analyzer. The intra-run and inter-run precisions were both <7.2%. The assay was linear from 0.2 to 4 μg/ml. The free phenytoin assay on the Cobas c501 was compared with the FPIAassay on the TDx analyzer using sera from 25 patients receiving phenytoin (phenytoin concentration between 0.3 and 3.7 μg/ml). The following regression equation was observed: y = 0.9899 x + 0.0408 (r = 0.98, n = 25). In conclusion, the free phenytoin assay on the Cobas c501 analyzer is a valid alternative to free phenytoin assay on the TDx analyzer.

journal_name

J Clin Lab Anal

authors

Dasgupta A,Davis B,Chow L

doi

10.1002/jcla.21536

subject

Has Abstract

pub_date

2013-01-01 00:00:00

pages

1-4

issue

1

eissn

0887-8013

issn

1098-2825

journal_volume

27

pub_type

杂志文章
  • The SNP43 (G/A) polymorphism in CAPN10 gene confers an increased risk of cognitive impairment in cerebral small vessel disease.

    abstract:BACKGROUND:Cognitive impairment, significantly reducing processing speed and executive function, is the critical consequence of cerebral small vessel disease (SVD), in which genetic variations have been studied. In this study, we explore the role of SNP43 (G/A) and SNP63 (C/T) polymorphism in the CAPN10 on cognitive im...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.22615

    authors: Wu K,Cai Y

    更新日期:2018-11-01 00:00:00

  • The microarray identification circular RNA hsa_circ_0105015 up-regulated involving inflammation pathway in essential hypertension.

    abstract:BACKGROUND:Essential hypertension (EH) is an inflammatory disease, and endothelial dysfunction induced by chronic inflammation is one of the pathogeneses of EH. The expression of some inflammatory mediators may be regulated by the interaction of circular RNAs (circRNAs) and microRNAs (miRNAs). METHODS:An Agilent human...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.23603

    authors: He X,Bao X,Tao Z,Sun J,Zheng S,Zhong F,Zhang L

    更新日期:2020-11-24 00:00:00

  • Serum lipid hydroperoxide levels and paraoxonase activity in patients with lung, breast, and colorectal cancer.

    abstract:OBJECTIVES:The objectives of this article are to investigate the serum lipid hydroperoxide (LOOH) levels and paraoxonase-1 (PON1) and arylesterase (ARE) activity in patients with lung, breast, and colorectal cancer. DESIGN AND METHODS:Serum PON1 and ARE activities and LOOH levels were measured in 110 patients with can...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.21503

    authors: Balci H,Genc H,Papila C,Can G,Papila B,Yanardag H,Uzun H

    更新日期:2012-05-01 00:00:00

  • Comparison of a New and Rapid Method: Brucella Coombs Gel Test With Other Diagnostic Tests.

    abstract:BACKGROUND:The aim of this study was to detect reliability of Brucella Coombs gel test (BCGT) by comparing with with ELISA (IgG + IgM), Standard agglutination test, and Brucella immunocapture agglutination methods in serological diagnosis of brucellosis. METHODS:Brucella Coombs gel test (BCGT), Brucella ELISA (IgG + I...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.21934

    authors: Kalem F,Ergün AG,Durmaz S,Doğan M,Ertuğrul Ö,Gündem S

    更新日期:2016-09-01 00:00:00

  • Analysis of serum cytokine levels in primary biliary cirrhosis patients and healthy adults.

    abstract::By using commercially available ELISA kits, serum IL-6 and TNF-alpha levels in healthy adults, and the levels of various cytokines in patients with primary biliary cirrhosis or chronic viral liver diseases, were investigated. IL-6 levels of healthy subjects were distributed in a wide range, and the distribution patter...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:

    authors: Yamashiki M,Kosaka Y,Nishimura A,Watanabe S,Nomoto M,Ichida F

    更新日期:1998-01-01 00:00:00

  • Proteinase K inactivation of cytosolic aspartate aminotransferase isoenzyme for measurement of human serum mitochondrial aspartate aminotransferase.

    abstract::We studied a new proteinase K assay method for human serum mitochondrial aspartate aminotransferase. We found that proteinase K showed no inactivation of human mitochondrial aspartate aminotransferase isoenzyme and complete inactivation of cytosolic aspartate aminotransferase. Previous studies have shown that selectiv...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.1860070202

    authors: Watazu Y,Uji Y,Okabe H,Shirahase Y,Kaneda N,Karmen A

    更新日期:1993-01-01 00:00:00

  • Reliability of low-avidity IgG and of IgA in the diagnosis of primary infection by rubella virus with adaptation of a commercial test.

    abstract::The detection of IgA and low-avidity IgG and antibodies in serum is a potentially useful marker of recent infection by a microorganism. We studied the reliability of IgG avidity and presence of IgA for the diagnosis of recent acute infection by rubella virus. Low-avidity IgG (Avy-EIA test) was determined with a modifi...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:

    authors: Gutiérrez J,Rodríguez MJ,De Ory F,Piédrola G,Maroto MC

    更新日期:1999-01-01 00:00:00

  • Serum levels of neopterin and interleukin-2 receptor in women with severe preeclampsia.

    abstract::Preeclampsia continues to be a major cause of maternal and perinatal mortality and morbidity worldwide. The etiopathogenesis of preeclampsia is not fully understood. Neopterin and interleukin-2 (IL-2) production reflects cellular immunity. Our purpose was to determine the levels of neopterin and interleukin-2 receptor...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.20053

    authors: Kaleli I,Kaleli B,Demir M,Yildirim B,Cevahir N,Demir S

    更新日期:2005-01-01 00:00:00

  • Angiotoxicity and atherogenicity of cholesterol oxides.

    abstract::Cholesterol in the diet can readily autoxidize and be absorbed and transported in plasma lipoproteins. Cholesterol oxides can also be endogenously produced in tissues via free-radical-induced reactions. Some cholesterol oxides, notably cholestane-3 beta, 5 alpha, 6 beta-triol and 25-hydroxycholesterol, have been shown...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章,评审

    doi:10.1002/jcla.1860050212

    authors: Peng SK,Hu B,Morin RJ

    更新日期:1991-01-01 00:00:00

  • The impact of IGF-I gene polymorphisms on coronary artery disease susceptibility.

    abstract:BACKGROUND:Coronary artery disease (CAD) was the second leading cause of death for the past 3 years in Taiwan. The insulin-like growth factor (IGF) system is considered a new risk factor of CAD because investigations show that the levels and bioactivity of IGF-I and IGFBP-3 (where IGFBP is insulin-like growth factor-bi...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.21581

    authors: Lin HL,Ueng KC,Wang HL,Chen TP,Yang SF,Chu SC,Hsieh YS

    更新日期:2013-03-01 00:00:00

  • High level of D-dimer predicts ischemic stroke in patients with infective endocarditis.

    abstract:BACKGROUND:Ischemic stroke is one of the most prominent and serious neurological complications of infective endocarditis (IE). Our study was designed to evaluate the predictive value of higher level of plasma D-dimer on admission for the development of ischemic stroke in patients with IE. METHODS:In this prospective s...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.23206

    authors: Xu N,Fu Y,Wang S,Li S,Cai D

    更新日期:2020-05-01 00:00:00

  • Performance Evaluation of a Salivary Amylase Biosensor for Stress Assessment in Military Field Research.

    abstract:BACKGROUND:A convenient biosensor for real-time measurement of biomarkers for in-field psychophysiological stress research and military operations is desirable. We evaluated a hand-held device for measuring salivary amylase as a stress marker in medical technicians undergoing combat casualty care training using two dif...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.21840

    authors: Peng HT,Savage E,Vartanian O,Smith S,Rhind SG,Tenn C,Bjamason S

    更新日期:2016-05-01 00:00:00

  • The impact of vascular endothelial growth factor +405 C/G polymorphism on long-term outcome and severity of coronary artery disease.

    abstract:BACKGROUND:The association between genetic variations of vascular endothelial growth factor (VEGF) gene and the risk for atherosclerosis has been hypothesized. We aimed to assess the relationship between rs2010963 (+405 C/G) polymorphism and presence, severity, and outcome of coronary artery disease (CAD) in an Iranian...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.22066

    authors: Kalayi Nia S,Ziaee S,Boroumand MA,Sotudeh Anvari M,Pourgholi L,Jalali A

    更新日期:2017-07-01 00:00:00

  • Study of serum argininosuccinate lyase determination for diagnosis of liver diseases.

    abstract::The objectives of this research were to establish an automatic analysis method for the determination of serum argininosuccinate lyase (ASL) and to investigate the value of serum ASL test in the diagnosis of various liver disorders. According to the chemical reaction catalyzed by ASL, an enzyme-coupled reaction system ...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.20245

    authors: Feng JF,Chen TM,Wen YA,Wang J,Tu ZG

    更新日期:2008-01-01 00:00:00

  • Decorin transfection in human mesangial cells downregulates genes playing a role in the progression of fibrosis.

    abstract::The proteoglycan decorin inhibits TGF-beta; therefore, it could antagonize progression of fibrotic diseases associated with activation of TGF-beta(1). The effect of decorin transfection in human mesangial cells (HMCs) on the expression of genes related to kidney fibrosis was investigated. HMCs, isolated from glomeruli...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.10038

    authors: Costacurta A,Priante G,D'Angelo A,Chieco-Bianchi L,Cantaro S

    更新日期:2002-01-01 00:00:00

  • Association of CD44 polymorphisms and susceptibility to HBV-related hepatocellular carcinoma in the Chinese population.

    abstract:BACKGROUND:This study aimed to determine whether CD44 polymorphisms were correlated with hepatocellular carcinoma (HCC) and to reveal a new potential target for early prediction, prevention, and diagnosis of HCC. METHOD:This study involved 96 cases with chronic hepatitis B (CHB), 96 cases with hepatitis B virus-relate...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.22977

    authors: Deng Y,Chen ZJ,Lan F,He QT,Chen SY,Du YF,Li S,Qin X

    更新日期:2019-10-01 00:00:00

  • Reference Intervals of and Relationships among Essential Trace Elements in Whole Blood of Children Aged 0-14 years.

    abstract:BACKGROUND:Essential trace elements are vital to human health. In this study, our aim was to establish reference intervals of and to evaluate relationships among Ca, Fe, Cu, Mg, and Zn for children. METHODS:We collected blood samples of 3210 children aged 0-14 years from Lu'an, China, and concentrations of the above e...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.22043

    authors: Zhai R,Zhang M,Liu J,Guang H,Li B,Chen D,Zhang S

    更新日期:2017-03-01 00:00:00

  • Reduced expression of circRNA hsa_circ_0067582 in human gastric cancer and its potential diagnostic values.

    abstract:BACKGROUND:Gastric cancer (GC) is one of the global mortality diseases and has a poor prognosis due to the lack of ideal tumor biomarkers. Circular RNAs (circRNAs) are an abundant kind of endogenous RNAs that recently are found play a crucial role in the cancer occurrence and development. Nevertheless, little is known ...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.23080

    authors: Yu X,Ding H,Yang L,Yu Y,Zhou J,Yan Z,Guo J

    更新日期:2020-03-01 00:00:00

  • Immunochromatographic assay to detect α-tubulin in urine for the diagnosis of kidney injury.

    abstract:BACKGROUNDS:Shortening of primary cilia in kidney epithelial cells is associated with kidney injury and involved with the induced level of α-tubulin in urine. Therefore, rapid detection and quantification of α-tubulin in the urine samples could be used to the preliminary diagnosis of kidney injury. METHODS:Cellulose-b...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.23015

    authors: Choi ES,Al Faruque H,Kim JH,Cho JH,Park KM,Kim E

    更新日期:2020-01-01 00:00:00

  • Differentiation of myeloperoxidase and glandular peroxidase in biological fluids: application to human saliva.

    abstract::A modification of the assay system for peroxidase activity in human mixed saliva using 2,2'-azinobis-(-ethylbenzthiazoline-6-sulphonic acid) (ABTS) as the substrate is reported. The modifications permit differentiation between glandular peroxidase (SPX) and myeloperoxidase (MPX) activity. In addition, factors endogeno...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.1860050111

    authors: Venezie RD,Jenzano JW,Lundblad RL

    更新日期:1991-01-01 00:00:00

  • The clinical value of lncRNA MALAT1 and its targets miR-125b, miR-133, miR-146a, and miR-203 for predicting disease progression in chronic obstructive pulmonary disease patients.

    abstract:OBJECTIVE:The study aimed to explore the correlations of long non-coding RNA MALAT1 (lncRNA MALAT1) and its targets microRNA (miR)-125b, miR-133, miR-146a, and miR-203 with acute exacerbation risk, inflammation, and disease severity of chronic obstructive pulmonary disease (COPD). METHODS:Plasma samples were obtained ...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.23410

    authors: Liu S,Liu M,Dong L

    更新日期:2020-09-01 00:00:00

  • Histocompatibility screening by molecular techniques: use of polymerase chain reaction products and heteroduplex formation.

    abstract::Novel molecular approaches have recently become available allowing improved major histocompatibility complex (MHC) matching of potential allogeneic bone marrow donors and recipients. Current cellular and serological assays are hindered by aberrant cell populations and limited reagents which only detect an individuals'...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.1860060515

    authors: Rubocki RJ,Wisecarver JL,Hook DD,Cox SM,Beisel KW

    更新日期:1992-01-01 00:00:00

  • Decreased serum exosomal miR-29a expression and its clinical significance in papillary thyroid carcinoma.

    abstract:BACKGROUND:Aberrant levels of circulating microRNAs (miRNAs) are potential biomarkers in papillary thyroid carcinoma (PTC) diagnosis and therapy. The aim of this study was to evaluate serum exosomal miR-29a expression as a non-invasive biomarker for PTC diagnosis and prognosis. METHODS:Quantitative reverse transcripti...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.23560

    authors: Wen Q,Wang Y,Li X,Jin X,Wang G

    更新日期:2020-12-23 00:00:00

  • Insulin-like growth factor-II gene polymorphism is associated with primary open angle glaucoma.

    abstract::Hypoxia and ischemia play important roles in the onset and progression of glaucoma. Insulin-like growth factors (IGF) are important neurotrophic agents that respond to hypoxia-ischemia. In this study, we enrolled 60 primary open angle glaucoma (POAG) patients and 104 healthy volunteers from the China Medical College H...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.10106

    authors: Tsai FJ,Lin HJ,Chen WC,Chen HY,Fan SS

    更新日期:2003-01-01 00:00:00

  • Enterovirus meningitis in Greece from 2003-2005: diagnosis, CSF laboratory findings, and clinical manifestations.

    abstract::Enteroviruses (EVs) are the most commonly identified cause of aseptic meningitis. Rapid detection and characterization of EV meningitis is essential in making decisions for patient management and treatment. A total of 52 cases of acute aseptic meningitis that occurred from March 2003 to April 2005 were investigated fo...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.20129

    authors: Dumaidi K,Frantzidou F,Papa A,Diza E,Antoniadis A

    更新日期:2006-01-01 00:00:00

  • Hormonal modulation of the vaginal bacterial flora in experimental polycystic ovarian disease.

    abstract::Rats exposed to constant light develop polycystic ovarian (PCO) disease with persistent estrus, representing an estrogen-dominant condition. Herein, we report that fluctuations seen in the vaginal microflora in cyclic rats were not observed in PCO rats with persistent estrus. The vaginal-cervical mucosa of PCO rats sh...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/(SICI)1098-2825(1996)10:5<233::AID-JCLA1>3

    authors: Singh KB,Mahajan DK,Tewari RP

    更新日期:1996-01-01 00:00:00

  • Scanning for α-Hemoglobin Variants by High-Resolution Melting Analysis.

    abstract:BACKGROUND:Definitive detection of hemoglobin (Hb) variants requires DNA sequencing. High-resolution melting (HRM) analysis of polymerase chain reaction (PCR) amplicons was applied to detect and discriminate among uncommon α-Hb variants found in Thailand. METHODS:Uncommon suspected α-Hb variants observed in Hb typing ...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.21914

    authors: Yimniam W,Jindadamrongwech S

    更新日期:2016-09-01 00:00:00

  • Associations of urinary, glomerular, and tubular markers with the development of diabetic kidney disease in type 2 diabetes patients.

    abstract:OBJECTIVES:To evaluate the associations of urinary markers (eg albumin), glomerular (eg transferrin [TRF], immunoglobulin G [IgG]), and tubular (eg α1-microglobulin [α1-MG], β2-microglobulin [β2-MG]) markers with the development of diabetic kidney disease (DKD) in type 2 diabetes patients, as assessed by estimated glom...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.22191

    authors: Jiang X,Zhang Q,Wang HB,Cui XF,Liu R

    更新日期:2018-01-01 00:00:00

  • Measurement of free and total hydroxyproline by automated flow injection of serum or urine samples from maintenance hemodialysis patients with renal osteodystrophy.

    abstract::An automated measurement of total and free hydroxyproline in serum or urine is presented that uses flow injection analysis. After exclusion of nonspecific substances, hydroxyproline was oxidized by chloramine-T and L-cysteine with Ehrlich's reagent. The linearity obtained was from 3.8 mumole/L to 1.22 mmole/L with goo...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.1860080504

    authors: Uji Y,Karmen A,Okabe H,Hata K,Miura M,Ozaki K,Minamizaki M,Shibata T,Inayama S

    更新日期:1994-01-01 00:00:00

  • New molecular diagnostic tests for two congenital forms of anemia.

    abstract::Congenital hypoplastic anemias are a rare and heterogeneous group of disorders. This paper reviews new molecular diagnostic tests in two distinct forms of congenital anemias, anemia due to transplacental infection with B19 parvovirus and Fanconi anemia. In both instances, molecular assays making use of amplification o...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章,评审

    doi:

    authors: Auerbach AD,Verlander PC,Brown KE,Liu JM

    更新日期:1997-01-01 00:00:00