Comparison of three commonly used PCR-based techniques to analyze MSI status in sporadic colorectal cancer.

abstract：:Gross cystic breast disease (GCBD) is the most common benign disease of the human female breast, and patients with GCBD have an increased risk of breast cancer. The aim of this study was to evaluate the distribution inside apocrine cells and in breast cyst fluids aspirated from gross cysts of prostate-specific antigen...

journal_title：Journal of clinical laboratory analysis

authors： Malatesta M,Mannello F,Sebastiani M,Gazzanelli G

更新日期：2001-01-01 00:00:00

abstract：BACKGROUND:Delta neutrophil index (DNI) is a new inflammatory marker and the present study aimed to evaluate the predictive value of the DNI for the presence of a perforation in elderly with acute appendicitis. METHODS:This retrospective observational study was conducted on 108 consecutive elderly patients (≥65 years ...

journal_title：Journal of clinical laboratory analysis

authors： Shin DH,Cho YS,Kim YS,Ahn HC,Oh YT,Park SO,Won MH,Cho JH,Kim YM,Seo JY,Lee YH

更新日期：2018-01-01 00:00:00

abstract：:In this study, the effect of the synthetic glucocorticoid hormone dexamethasone (DXM) on HSV replication was studied in a DXM receptor-positive mouse neuroblastoma (NB) cell line. In cells treated with 10(-7) M DXM and then infected with HSV, there was a statistically significant 9-18-fold increase in the amount of vi...

journal_title：Journal of clinical laboratory analysis

authors： Sawiris GP,Sydiskis RJ,Bashirelahi N

更新日期：1994-01-01 00:00:00

abstract：:Mycobacterium tuberculosis bacilli are seldom demonstrated in tuberculous pleural effusion (TPE) by conventional bacteriological methods. In this study, an indirect enzyme-linked immunosorbent assay (ELISA) was developed to detect IgG against four mycobacterial recombinant antigens (ESAT-6, PlcA, HspX and Tb8.4) in 69...

journal_title：Journal of clinical laboratory analysis

authors： Sumi S,Radhakrishnan VV

更新日期：2010-01-01 00:00:00

abstract：:The immune complex transfer enzyme immunoassay for antibody IgG to HIV-1 gp41 antigen was developed using two synthetic peptides. An aliquot (10 microl) of serum samples from HIV-1 seropositive subjects was incubated simultaneously with 2,4-dinitrophenyl-bovine serum albumin-synthetic HIV-1 gp41 peptide conjugates and...

journal_title：Journal of clinical laboratory analysis

authors： Hashida S,Ishikawa S,Hashinaka K,Nishikata I,Oka S,Ishikawa E

更新日期：1998-01-01 00:00:00

abstract：BACKGROUND:The GABA-A receptor signaling pathway regulates proliferation, differentiation, apoptosis, and responses to overt DNA damage during embryonic development. METHODS:To analyze the gene expression after intracytoplasmic sperm injection (ICSI) and in in vivo mouse embryos, the global pattern of gene expression ...

journal_title：Journal of clinical laboratory analysis

authors： Zhang Z,Wang T,Huang J,Huang Y,Zhang Q

更新日期：2020-09-20 00:00:00

abstract：BACKGROUND:Although recent studies have indicated that gut microbiome dysbiosis was significantly associated with the onset of type 2 diabetes mellitus (T2DM), information on the role of blood microbiome in T2DM development is scarce. METHODS:Fifty incident T2DM cases and 100 matched non-T2DM controls were selected fr...

journal_title：Journal of clinical laboratory analysis

authors： Qiu J,Zhou H,Jing Y,Dong C

更新日期：2019-05-01 00:00:00

abstract：BACKGROUND:We propose a simple, sensitive, and fast high-performance liquid chromatography ultraviolet detection (HPLC-UV) method for the quantitative determination of bosutinib in human plasma. METHODS:Plasma samples were processed using an Oasis hydrophilic-lipophilic balance extraction cartridge (1 mL, 30 mg). Bosu...

journal_title：Journal of clinical laboratory analysis

authors： Sumimoto T,Nakahara R,Sato Y,Itoh H

更新日期：2018-01-01 00:00:00

abstract：:The matrix metalloproteinase matrilysin (MMP-7) is a member of the matrix metalloproteinase gene family, which is believed to play an important role in tumor invasion and metastasis. We have previously found that matrilysin mRNA is specifically expressed in colorectal cancers and adenomas and that its message is local...

journal_title：Journal of clinical laboratory analysis

authors： Yamamoto H,Itoh F,Senota A,Adachi Y,Yoshimoto M,Endoh T,Hinoda Y,Yachi A,Imai K

更新日期：1995-01-01 00:00:00

abstract：BACKGROUND:Gastric cancer (GC) is one of the global mortality diseases and has a poor prognosis due to the lack of ideal tumor biomarkers. Circular RNAs (circRNAs) are an abundant kind of endogenous RNAs that recently are found play a crucial role in the cancer occurrence and development. Nevertheless, little is known ...

journal_title：Journal of clinical laboratory analysis

authors： Yu X,Ding H,Yang L,Yu Y,Zhou J,Yan Z,Guo J

更新日期：2020-03-01 00:00:00

abstract：BACKGROUND:Kawasaki disease (KD) is an acute, self-limited vasculitis in infants and young children. Interleukin-10 (IL-10) is a potent cytokine that exerts pleiotropic effects on immunoregulation and inflammation. Elevated IL-10 serum levels have been reported in the KD patients. METHODS:In this study, we investigate...

journal_title：Journal of clinical laboratory analysis

authors： Lin YJ,Lan YC,Lai CH,Lin TH,Huang SM,Liao CC,Lin CW,Hung CH,Tien N,Liu X,Chien WK,Chen JH,Tsai FJ

更新日期：2014-11-01 00:00:00

abstract：BACKGROUND:This study was aimed to investigate whether measurement of free testosterone and cortisol in saliva is a reliable alternative to their assessment in serum for monitoring physical fitness in professional athletes. METHODS:We studied 25 members of the soccer team Parma F.C., playing in Italian major football ...

journal_title：Journal of clinical laboratory analysis

authors： Lippi G,Dipalo M,Buonocore R,Gnocchi C,Aloe R,Delsignore R

更新日期：2016-09-01 00:00:00

abstract：BACKGROUND:Limited data are available for the diagnostic value, and for the diagnostic sensitivity and specificity of joint detection of serum lactate dehydrogenase (sLDH)/pleural fluid adenosine deaminase (pADA) and pleural fluid carcinoembryonic antigen (pCEA) in malignant pleural effusion (MPE). METHODS:We collecte...

journal_title：Journal of clinical laboratory analysis

authors： Zhang F,Hu L,Wang J,Chen J,Chen J,Wang Y

更新日期：2017-09-01 00:00:00

abstract：:The detection of IgA and low-avidity IgG and antibodies in serum is a potentially useful marker of recent infection by a microorganism. We studied the reliability of IgG avidity and presence of IgA for the diagnosis of recent acute infection by rubella virus. Low-avidity IgG (Avy-EIA test) was determined with a modifi...

journal_title：Journal of clinical laboratory analysis

authors： Gutiérrez J,Rodríguez MJ,De Ory F,Piédrola G,Maroto MC

更新日期：1999-01-01 00:00:00

abstract：BACKGROUND:Low concentration C-reactive protein (CRP) has favorable prognostic significance in patients with cardiovascular risks. METHODS:We compared the wr-CRP method with the hs-CRP method both on Roche Cobas c702 analyzer for the determination of low CRP concentration (<20 mg/L) including 200 patients treated in C...

journal_title：Journal of clinical laboratory analysis

authors： Li R,Xue Y,Wang T,Gong L,Peng P,Xiong P,Dai M,Shao T,Hu Y,Ye X

更新日期：2019-10-01 00:00:00

abstract：:Adenosine deaminase (ADA) exists as two isoenzymes, ADA(1) and ADA(2). It appears that the ADA(2) isoenzyme originates mainly from monocytes and macrophages. In tuberculous pleural effusions most of the ADA activity consists of ADA(2). The aim of this prospective study was to analyse ADA isoenzymes in the CSF of patie...

journal_title：Journal of clinical laboratory analysis

authors： Schutte CM,Ungerer JP,du Plessis H,van der Meyden CH

更新日期：2001-01-01 00:00:00

abstract：:Mycobacterium avium is an important intracellular pathogen, particularly in AIDS patients. It also shows the second frequency among nontuberculous mycobacteria infections in Korea. Point mutations of domain V region of the 23S rRNA gene has been known to confer clarithromycin resistance to M. avium. In order to isolat...

journal_title：Journal of clinical laboratory analysis

authors： Lee SH,Park YK,Kim CK,Kim HJ

更新日期：2011-01-01 00:00:00

abstract：:Previous studies have shown elevated serum levels of interleukin 6 (IL-6) and C-reactive protein (CRP) in patients with pancreatic cancer (PC). The aim of this study was to assess the diagnostic usefulness of pretreatment serum levels of IL-6 and CRP to differentiate between PC and chronic pancreatitis (CP) patients. ...

journal_title：Journal of clinical laboratory analysis

authors： Mroczko B,Groblewska M,Gryko M,Kedra B,Szmitkowski M

更新日期：2010-01-01 00:00:00

abstract：:We report nine donations with ABO inconsistency in reverse typing caused by partly or entirely missing antibodies. A and B antigens and antibodies were examined by serological blood typing, and ABO deoxyribonucleic acid (DNA) analyses were performed by sequence-specific priming and sequencing. A B101 allele was demons...

journal_title：Journal of clinical laboratory analysis

authors： Deng ZH,Zeng JQ,Yu Q,Su YQ,Liang YL,Lu L,Zhu WG,Yang BC

更新日期：2007-01-01 00:00:00

abstract：BACKGROUND:Primary adrenal lymphoma (PAL) is an infrequent malignant disease and there is no consensus classification or specialized treatment for it. However, PAL has been observed to have worse prognosis compared with other extrarenal malignant lymphomas and diffuse large B-cell lymphoma presents as the most common s...

journal_title：Journal of clinical laboratory analysis

authors： Chen Y,He HS,Hu QF,Wang G

更新日期：2020-05-01 00:00:00

abstract：:Congenital heart disease (CHD) is a common cardiac defect found in infants and children. Despite advances in diagnosis and treatment, our understanding of the causative mechanism and etiology of CHD is limited. To determine the genetic etiology of CHD, we selected 11 consecutive short tandem-repeat polymorphic (STRP) ...

journal_title：Journal of clinical laboratory analysis

authors： Shi YR,Hsieh KS,Wu JY,Lee CC,Tsai CH,Yu MT,Chang JS,Tsai FJ

更新日期：2003-01-01 00:00:00

abstract：BACKGROUND:To discover how NLRP3 and TNFRSF1A polymorphisms affect the efficacy of traditional medicine and etanercept for ankylosing spondylitis (AS) patients. METHODS:Single nucleotide polymorphism (SNP) and haplotype analyses were conducted based on determined NLRP3 and TNFRSF1A among AS patients. We subsequently a...

journal_title：Journal of clinical laboratory analysis

authors： Zhao S,Chen H,Wu G,Zhao C

更新日期：2017-11-01 00:00:00

abstract：BACKGROUND:Identification of dermatophytes at the species level, relying on macro- and microscopic properties of the colonies is time-consuming, questioned in many circumstances, and requires considerable expertise. In this study, we examined the potency of a new genetic marker, β-tubulin (BT2) gene, for differentiatio...

journal_title：Journal of clinical laboratory analysis

authors： Abastabar M,Mirhendi H,Rezaei-Matehkolaei A,Shidfar MR,Kordbacheh P,Makimura K

更新日期：2014-03-01 00:00:00

abstract：BACKGROUND:Nucleolar and spindle-associated protein 1 (NUSAP1) has been identified to be strongly implicated in the carcinogenesis of cervical carcinoma, breast cancer, and liver cancer, and shows a high expression level in bladder cancer, indicating that NUSAP1 might be a potent target for cancer treatment. Using bioi...

journal_title：Journal of clinical laboratory analysis

authors： Chen Y,Zhang W,Kadier A,Zhang H,Yao X

更新日期：2020-05-01 00:00:00

abstract：:Antibodies to neutrophil cytoplasmic antigens (ANCA) targeted toward granule enzymes have been recognized as a valuable diagnostic tool in the detection of Wegener's granulomatosis and systemic vasculitides. However, the most commonly used method of detection, the indirect immunofluorescence assay, is prone to false-p...

journal_title：Journal of clinical laboratory analysis

authors： Streicher J,Fabian B,Herkner K,Pointner H,Bayer PM

更新日期：1998-01-01 00:00:00

abstract：INTRODUCTION:The Mindray CAL 8000 is a cellular analysis line that consists of the BC-6800, an automated hematology analyzer, and the SC-120, an automated slidemaker/stainer. We evaluated the performances of the BC-6800 and the SC-120. METHODS:Four hundred and eight normal and abnormal samples were analyzed. The perfo...

journal_title：Journal of clinical laboratory analysis

authors： Lee HT,Park PW,Seo YH,Kim KH,Seo JY,Jeong JH,Kim MJ,Ahn JY

更新日期：2017-07-01 00:00:00

abstract：BACKGROUND:Automated cell counters have become more and more sophisticated with passing years. The numerical and graphic data both provide useful clues for suspecting a diagnosis especially when the workload is very high. AIM:We present our experience of useful information provided by graphic displays of an automated ...

journal_title：Journal of clinical laboratory analysis

authors： Gupta M,Chauhan K,Singhvi T,Kumari M,Grover RK

更新日期：2018-06-01 00:00:00

abstract：BACKGROUND:Normal serum alanine aminotransferase (ALT) levels differ with age, gender, and body mass index. Adjusting the upper limits of normal (ULN) for ALT needs further research in different populations. Aim of this work was to monitor the effect of successful chronic hepatitis C (CHC) treatment on the ALT levels i...

journal_title：Journal of clinical laboratory analysis

authors： El Kassas M,Alboraie M,Mostafa A,Ezzat R,El Tahan A,Afify S,Sweedy A,Kabbash I,Esmat G

更新日期：2018-03-01 00:00:00

abstract：:Vitamin D plays a vital role not only in bone health but also in pathophysiology of many other body functions. In recent years, there has been significant increase in testing of 25-hydroxyvitamin D (25-OH vitamin D), a marker of vitamin D deficiency. The most commonly used methods for the measurement of 25-OH vitamin ...

journal_title：Journal of clinical laboratory analysis

authors： Garg U,Munar A,Frazee C 3rd,Scott D

更新日期：2012-09-01 00:00:00

abstract：BACKGROUND:Today, the genetic and genomic research entered in a new era of high-throughput genotyping technology. However, mutagenic polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) is still a choice of genotyping method in molecular epidemiological research. It has been extensively used fo...

journal_title：Journal of clinical laboratory analysis

authors： Sharma N,Awasthi S,Phadke SR

更新日期：2016-03-01 00:00:00