Reliability of low-avidity IgG and of IgA in the diagnosis of primary infection by rubella virus with adaptation of a commercial test.

abstract：:For many years, fluorescence polarization immunoassay (FPIA) on the TDx analyzer has been used for determination of free phenytoin concentration. Recently Abbott Laboratories decided to discontinue the TDx analyzer and related assays on this analyzer. Free phenytoin assay is also available from Roche Diagnostics for a...

journal_title：Journal of clinical laboratory analysis

authors： Dasgupta A,Davis B,Chow L

更新日期：2013-01-01 00:00:00

abstract：:Enteroviruses (EVs) are the most commonly identified cause of aseptic meningitis. Rapid detection and characterization of EV meningitis is essential in making decisions for patient management and treatment. A total of 52 cases of acute aseptic meningitis that occurred from March 2003 to April 2005 were investigated fo...

journal_title：Journal of clinical laboratory analysis

authors： Dumaidi K,Frantzidou F,Papa A,Diza E,Antoniadis A

更新日期：2006-01-01 00:00:00

abstract：:A modification of the assay system for peroxidase activity in human mixed saliva using 2,2'-azinobis-(-ethylbenzthiazoline-6-sulphonic acid) (ABTS) as the substrate is reported. The modifications permit differentiation between glandular peroxidase (SPX) and myeloperoxidase (MPX) activity. In addition, factors endogeno...

journal_title：Journal of clinical laboratory analysis

authors： Venezie RD,Jenzano JW,Lundblad RL

更新日期：1991-01-01 00:00:00

abstract：BACKGROUND:Coronary heart disease (CHD) is primarily caused by atherosclerosis of coronary arteries. It is largely an inflammatory disease of the vascular wall. The inflammation is related to DNA methylation. Angiopoietin-like protein 2 (ANGPTL2) has various functions in several chronic inflammatory diseases. Macrophag...

journal_title：Journal of clinical laboratory analysis

authors： Zhou J,Chen L,Yang X,Huang X,Wang Z,Peng P,Lian J

更新日期：2019-03-01 00:00:00

abstract：:Recently, Siemens Diagnostics released a new calcium assay (CA_2) based on complex formation of calcium with Arsenazo III dye for use on the three automated, random access ADVIA Chemistry analyzers (1650, 2400, and 1200). We evaluated this method for analytical performance as well as potential interference from gadoli...

journal_title：Journal of clinical laboratory analysis

authors： Datta P,Dasgupta A

更新日期：2009-01-01 00:00:00

abstract：BACKGROUND:Prototheca species are a group of organisms ubiquitously existing in nature but have become a pathogenic threat to public health, which has aroused wide attention. Species identification and antifungal susceptibility have essential and valuable meanings to clinical diagnosis and treatment. METHODS:A case of...

journal_title：Journal of clinical laboratory analysis

authors： Zhao F,Chen M,Fu Y

更新日期：2020-11-01 00:00:00

abstract：BACKGROUND:Gastric cancer (GC) is one of the global mortality diseases and has a poor prognosis due to the lack of ideal tumor biomarkers. Circular RNAs (circRNAs) are an abundant kind of endogenous RNAs that recently are found play a crucial role in the cancer occurrence and development. Nevertheless, little is known ...

journal_title：Journal of clinical laboratory analysis

authors： Yu X,Ding H,Yang L,Yu Y,Zhou J,Yan Z,Guo J

更新日期：2020-03-01 00:00:00

abstract：:Congenital hypoplastic anemias are a rare and heterogeneous group of disorders. This paper reviews new molecular diagnostic tests in two distinct forms of congenital anemias, anemia due to transplacental infection with B19 parvovirus and Fanconi anemia. In both instances, molecular assays making use of amplification o...

journal_title：Journal of clinical laboratory analysis

authors： Auerbach AD,Verlander PC,Brown KE,Liu JM

更新日期：1997-01-01 00:00:00

abstract：BACKGROUND:We propose a simple, sensitive, and fast high-performance liquid chromatography ultraviolet detection (HPLC-UV) method for the quantitative determination of bosutinib in human plasma. METHODS:Plasma samples were processed using an Oasis hydrophilic-lipophilic balance extraction cartridge (1 mL, 30 mg). Bosu...

journal_title：Journal of clinical laboratory analysis

authors： Sumimoto T,Nakahara R,Sato Y,Itoh H

更新日期：2018-01-01 00:00:00

abstract：BACKGROUND:Chronic hepatitis B (CHB) is one of the major infectious diseases in which CD4+ T helper (Th) cells play a crucial role. Th9 cells are a distinct subset of CD4+ Th cells with important physiological functions. OBJECTIVE:The study aimed to assess the potential involvement of Th9 cells in the inadequate immun...

journal_title：Journal of clinical laboratory analysis

authors： Cui M,Lv Y,Lu J,Zhang W,Duan Y,Huang Y,Yang L,Li M,Liu W,Liu D,Yan H

更新日期：2018-02-01 00:00:00

abstract：:Interleukin (IL)-6 and IL-8 were measured in 101 serum samples collected from eight intensive-care unit patients using a polystyrene-based stick enzyme-linked immunosorbent assay (STICKELISA) system. This system consisted of an immobilized-antibody ELISA stick and a noncontact spectrophotometer. Cytokine concentration...

journal_title：Journal of clinical laboratory analysis

authors： Miwa K,Shibayama N,Moriguchi T,Goto J,Yanagisawa M,Yamazaki Y,Jung G,Matsuda K

更新日期：2009-01-01 00:00:00

abstract：:Acid phosphatase was purified to electrophoretic homogeneity from human normal lung and spleen and was characterized biochemically and immunologically in comparison with prostate acid phosphatase (PAP). The apparent MW of lung acid phosphatase (LAP) and spleen acid phosphatase (SAP) was 110,000 and 100,000, respective...

journal_title：Journal of clinical laboratory analysis

authors： Lin MF,Li SS,Chu TM,Lee CL

更新日期：1990-01-01 00:00:00

abstract：BACKGROUND:In this study, the fresh stool samples from 254 children under 5 years of age with acute gastroenteritis which were delivered between October 2012 and December 2013 were collected. METHODS:In the stool samples, rotavirus antigens were investigated using two different immunochromatographic methods which are ...

journal_title：Journal of clinical laboratory analysis

authors： Artiran S,Atalay A,Gökahmetoglu S,Ozturk MA,Balci N,Cakir N,Kilic H,Durmaz R

更新日期：2017-03-01 00:00:00

abstract：OBJECTIVE:To explore relationships between immunological status, clinical features, radiographic damage, disease activity, and functional disability in Tunisian patients with rheumatoid arthritis (RA). MATERIALS AND METHODS:The study was carried out in 112 patients with RA. Demographic characteristics, disease duratio...

journal_title：Journal of clinical laboratory analysis

authors： Hamad MB,Marzouk S,Kaddour N,Masmoudi H,Fakhfakh F,Rebai A,Bahloul Z,Maalej A

更新日期：2014-01-01 00:00:00

abstract：:The technique that allows for in situ detection of PCR-amplified DNA and cDNA has improved remarkably quickly over the past 5 years. As of this writing, one can expect good results after a relatively quick "learning curve." The technique demands some skill in working with glass slides and cells/tissues. Thus, I recomm...

journal_title：Journal of clinical laboratory analysis

authors： Nuovo GJ

更新日期：1996-01-01 00:00:00

abstract：BACKGROUND:Fork head/winged helix transcription factor (Foxp3) plays a pivotal role in regulatory T (Treg) cells. The present study aimed to assess the association of Crohn's disease (CD) with Foxp3 polymorphisms and its colonic expression in Chinese patients. METHODS:The Foxp3 polymorphisms, rs3761547, rs2232365, rs2...

journal_title：Journal of clinical laboratory analysis

authors： Xia S,Zhang D,Zheng S,Wu C,Lin Q,Ying S,Shao X,Jiang Y

更新日期：2019-05-01 00:00:00

abstract：:N-terminal pro-brain natriuretic peptide (NT-proBNP) and BNP measurement could have a significant role in differentiating dyspnea between cardiac or pulmonary origin in the emergency room. The development of new and different commercial assays for these B-type natriuretic peptides offers the possibility of improving a...

journal_title：Journal of clinical laboratory analysis

authors： Sanz MP,Borque L,Rus A,Vicente B,Ramírez Y,Lasa L

更新日期：2006-01-01 00:00:00

abstract：:As several possible prognostic and therapeutic applications of interleukin-6 are currently under trial, the available methods for its quantification in biological fluids should be evaluated. In this report, the 7TD1 hybridoma bioassay is compared to an enzyme immunoassay for the determination of interleukin-6 in serum...

journal_title：Journal of clinical laboratory analysis

authors： Papadopoulos NG,Dedoussis GV,Baxevanis CN,Papamichail M

更新日期：1995-01-01 00:00:00

abstract：:The purpose of this study was to evaluate whether IL-1 beta (IL-1beta promoter and IL-1beta exon 5), IL-1 receptor antagonist (IL-1 Ra), and IL-4 (IL-4 promoter and IL-4 intron 3) gene polymorphisms act as markers of susceptibility to Kawasaki disease (KD), or of the severity of the disease. The study included 107 KD ...

journal_title：Journal of clinical laboratory analysis

authors： Wu SF,Chang JS,Wan L,Tsai CH,Tsai FJ

更新日期：2005-01-01 00:00:00

abstract：:The presence of high-molecular intestinal ALP (HIALP) overlapping with bone ALP in the alpha(2)beta region has been demonstrated. In this study we evaluated a method of separating HIALP after its conversion into ALP(5) by the action of protease. Serum samples from patients were mixed with protease at a ratio of 5:1 an...

journal_title：Journal of clinical laboratory analysis

authors： Ooi K,Shiraki K,Morishita Y,Nobori T

更新日期：2007-01-01 00:00:00

abstract：OBJECTIVES:Existing evidence suggests that impaired vitamin D metabolism contribute to the development of atherosclerosis. Aortic intima-media thickness (IMT) is an earlier marker than carotid IMT of preclinical atherosclerosis. However, there is a lack of researches on direct investigation of relevance between serum 2...

journal_title：Journal of clinical laboratory analysis

authors： Kalkan GY,Gür M,Koyunsever NY,Şeker T,Gözükara MY,Uçar H,Kaypaklı O,Baykan AO,Akyol S,Türkoğlu C,Elbasan Z,Şahin DY,Çaylı M

更新日期：2015-07-01 00:00:00

abstract：BACKGROUND:So far, there has been no tool to estimate activity at diagnosis and predict all-cause mortality in patients with ANCA-associated vasculitis (AAV). Hence, we determined the initial predictors of them in patients with AAV. METHODS:We retrospectively reviewed the medical records of 182 patients with AAV. Seve...

journal_title：Journal of clinical laboratory analysis

authors： Kim MK,Choi H,Kim JY,Song JJ,Park YB,Lee SW

更新日期：2020-01-01 00:00:00

abstract：:We found that the Rhabdomyosarcoma (RD) cell line expresses human acetylcholine receptor (AChR) based on the following evidences: 1. Soluble AChR can be isolated from RD cells following the isolation procedure for AChR from human muscle; 2. Intact RD cells bind to alpha-bungarotoxin (alpha Butx) in a time-dependent an...

journal_title：Journal of clinical laboratory analysis

authors： Wu JT,Astill M,Lloyd C,Salmon VC

更新日期：1993-01-01 00:00:00

abstract：BACKGROUND:Bone marrow (BM) study plays an important role as initial investigation specimen of lymphoma as well as staging lymphoma. This study aimed to investigate the utility of BM studies for classification of lymphoma and evaluate features of BM involvement by lymphoma over a period of 11 years. METHODS:A total of...

journal_title：Journal of clinical laboratory analysis

authors： Kwoun WJ,Ahn JY,Park PW,Seo YH,Kim KH,Seo JY,Lee HT,Yoo KH

更新日期：2019-05-01 00:00:00

abstract：BACKGROUND:Liver biopsy is recommended in the majority of patients with chronic viral hepatitis for fibrosis evaluation. Because of the potential risks of liver biopsy, many studies related to non-invasive biomarkers of hepatic fibrosis have been performed. We aimed to assess the diagnostic value of serum biglycan as a...

journal_title：Journal of clinical laboratory analysis

authors： Ciftciler R,Ozenirler S,Yucel AA,Cengiz M,Erkan G,Buyukdemirci E,Sönmez C,Esendaglı GY

更新日期：2017-09-01 00:00:00

abstract：BACKGROUND:Normal serum alanine aminotransferase (ALT) levels differ with age, gender, and body mass index. Adjusting the upper limits of normal (ULN) for ALT needs further research in different populations. Aim of this work was to monitor the effect of successful chronic hepatitis C (CHC) treatment on the ALT levels i...

journal_title：Journal of clinical laboratory analysis

authors： El Kassas M,Alboraie M,Mostafa A,Ezzat R,El Tahan A,Afify S,Sweedy A,Kabbash I,Esmat G

更新日期：2018-03-01 00:00:00

abstract：INTRODUCTION:Despite advances in diagnostic techniques, many cases of acute myeloid leukemia (AML) remain underdiagnosed in remote centers unequipped with these technologies. We hypothesized that the automated cellular indices with scatter plots and flags may aid in rapid and cost-effective screening of AML. METHODS:C...

journal_title：Journal of clinical laboratory analysis

authors： Virk H,Varma N,Naseem S,Bihana I,Sukhachev D

更新日期：2019-02-01 00:00:00

abstract：BACKGROUND:Contradictory results have been reported previously in the analyses of cross-reactivity among Blomia tropicalis (Blo t), Dermatophagoides pteronyssinus (Der p), and Dermatophagoides farinae (Der f). This study aims to investigate the characteristics of co-sensitization and the IgE cross-reactivity among them...

journal_title：Journal of clinical laboratory analysis

authors： Liu X,Zheng P,Zheng SG,Zhai Y,Zhao X,Chen Y,Cai C,Wu Z,Huang Z,Zou X,Liao C,Sun B

更新日期：2019-11-01 00:00:00

abstract：BACKGROUND:Information on serum albumin and trace elements among children suffering from Sickle Cell Anemia (SCA) was poorly documented in Africa. The aim of this study was to describe and to compare different values of trace elements with published reports from other parts of the world. METHODS:We carried out a case-...

journal_title：Journal of clinical laboratory analysis

authors： Sungu JK,Mukuku O,Mutombo AM,Mawaw P,Aloni MN,Luboya ON

更新日期：2018-01-01 00:00:00

abstract：BACKGROUND:Salmonella is an important foodborne pathogen that causes acute diarrhea in humans worldwide. This study analyzed the relationships of serotypes and antibiotic resistance with virulence genes of Salmonella isolated from children with salmonellosis. METHODS:Serological typing was performed using the slide-ag...

journal_title：Journal of clinical laboratory analysis

authors： Yue M,Li X,Liu D,Hu X

更新日期：2020-08-14 00:00:00