Abstract:
:A modification of the assay system for peroxidase activity in human mixed saliva using 2,2'-azinobis-(-ethylbenzthiazoline-6-sulphonic acid) (ABTS) as the substrate is reported. The modifications permit differentiation between glandular peroxidase (SPX) and myeloperoxidase (MPX) activity. In addition, factors endogenous to saliva such as thiocyanate did not adversely affect the modified assay system. The basic peroxidase assay utilized 1.0 mM ABTS and 0.1 mM H2O2 in 0.1 M sodium acetate, pH 4.7. The addition of 100 mM NaCl to the assay inhibited 90% of the activity of purified MPX but only 40% of the SPX activity in parotid saliva presumed to be free of MPX. Addition of Cl- to the assay had varied effects on the mixed saliva SPX activity (44-68% inhibition), suggesting that MPX is present in varying amounts in mixed saliva. Assay of mixed saliva from patients with different states of periodontal health indicated that the amount of Cl- inhibition is dependent on amount of inflammation and thus amount of MPX present. The results suggest that the modified assay yields a reliable estimate of relative SPX activity in mixed saliva.
journal_name
J Clin Lab Analjournal_title
Journal of clinical laboratory analysisauthors
Venezie RD,Jenzano JW,Lundblad RLdoi
10.1002/jcla.1860050111subject
Has Abstractpub_date
1991-01-01 00:00:00pages
57-9issue
1eissn
0887-8013issn
1098-2825journal_volume
5pub_type
杂志文章abstract:BACKGROUND:The aim of this study was to detect reliability of Brucella Coombs gel test (BCGT) by comparing with with ELISA (IgG + IgM), Standard agglutination test, and Brucella immunocapture agglutination methods in serological diagnosis of brucellosis. METHODS:Brucella Coombs gel test (BCGT), Brucella ELISA (IgG + I...
journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
doi:10.1002/jcla.21934
更新日期:2016-09-01 00:00:00
abstract:BACKGROUND:The aim of this study was to explain the effects of microRNA-132 in renal cell carcinoma by regulating FOXM1 expression. METHODS:Thirty patients with renal cell carcinoma admitted to our hospital were enrolled, and their adjacent normal tissues and cancer tissues were taken. The expression of microRNA-132 w...
journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
doi:10.1002/jcla.22969
更新日期:2020-01-01 00:00:00
abstract::The pathogenic role of immune-mediated mechanisms in chronic hepatitis C virus (HCV) infection has not yet been elucidated. In this study, we report different cytokine expression profiles from hemodialysis (HD) and non-HD HCV (+) patients. IL-1beta, IL-2, IL-4, IL-6, TNF-alpha, and TGF-beta1 serum levels, and liver bi...
journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
doi:10.1002/jcla.2060
更新日期:2002-01-01 00:00:00
abstract::We report nine donations with ABO inconsistency in reverse typing caused by partly or entirely missing antibodies. A and B antigens and antibodies were examined by serological blood typing, and ABO deoxyribonucleic acid (DNA) analyses were performed by sequence-specific priming and sequencing. A B101 allele was demons...
journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
doi:10.1002/jcla.20196
更新日期:2007-01-01 00:00:00
abstract:BACKGROUND:Reference intervals are critical for interpreting test results of a clinical laboratory. The aim of this study was to establish local reference intervals of complete blood count for healthy preschoolers in China. METHODS:Three thousand eight hundred and twenty-one blood specimens from children aged 4 months...
journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
doi:10.1002/jcla.22095
更新日期:2017-09-01 00:00:00
abstract:BACKGROUND:Miltenberger (Mi) series are the collective glycophorin hybrids in the MNS blood group system. Mi series are composed of several subtypes, for examples, GP.Mur, GP.Hop, and GP.Bun. The incompatibility of Mi series blood transfusion poses the risk of hemolysis. Due to the lack of standard antibodies for Mi se...
journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
doi:10.1002/jcla.21951
更新日期:2016-11-01 00:00:00
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journal_title:Journal of clinical laboratory analysis
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更新日期:2007-01-01 00:00:00
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journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
doi:10.1002/jcla.21676
更新日期:2014-07-01 00:00:00
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journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章,meta分析
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更新日期:2020-10-01 00:00:00
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journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
doi:10.1002/jcla.20395
更新日期:2010-01-01 00:00:00
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journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
doi:10.1002/jcla.20341
更新日期:2009-01-01 00:00:00
abstract::Diurnal variations in the excretion of bone resorption markers were assessed in order to identify the type of urine collection which provides the most information on bone resorption rate and its relation to measuring bone dynamics in a postmenopausal population. Sixty women, ages 43-67 and without disease or treatment...
journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
doi:10.1002/(sici)1098-2825(1998)12:3<145::aid-jcla3>3
更新日期:1998-01-01 00:00:00
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journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
doi:10.1002/jcla.23586
更新日期:2020-09-24 00:00:00
abstract:BACKGROUND:Delta neutrophil index (DNI) is a new inflammatory marker and the present study aimed to evaluate the predictive value of the DNI for the presence of a perforation in elderly with acute appendicitis. METHODS:This retrospective observational study was conducted on 108 consecutive elderly patients (≥65 years ...
journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
doi:10.1002/jcla.22177
更新日期:2018-01-01 00:00:00
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journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
doi:10.1002/jcla.20413
更新日期:2010-01-01 00:00:00
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journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
doi:10.1002/jcla.1860040613
更新日期:1990-01-01 00:00:00
abstract:BACKGROUND:The non-invasive diagnostic approach for early detection of endometrial cancer (EC) remains limited. To date, human epididymis protein 4 (HE4) has been intensively studied but its diagnostic is controversial in EC. DJ-1 is an oncoprotein secreted by cancer cells, recently identified as a potential diagnostic...
journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
doi:10.1002/jcla.22223
更新日期:2018-01-01 00:00:00
abstract:BACKGROUND:This study was performed to compare the clinical efficacies of anterior cruciate ligament (ACL) reconstruction with autologous ligament grafting at different time points. METHODS:Eighty-five patients with ACL were categorized into two groups: Group A (GA, n = 45), who underwent early-stage (≤3 weeks) surger...
journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
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更新日期:2020-08-26 00:00:00
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journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
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更新日期:2017-11-01 00:00:00
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journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
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journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2825(1996)10:1<1::AID-JCLA1>3.0
更新日期:1996-01-01 00:00:00
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journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
doi:10.1002/jcla.20216
更新日期:2008-01-01 00:00:00
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journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
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更新日期:2020-08-01 00:00:00
abstract::Antibodies to neutrophil cytoplasmic antigens (ANCA) targeted toward granule enzymes have been recognized as a valuable diagnostic tool in the detection of Wegener's granulomatosis and systemic vasculitides. However, the most commonly used method of detection, the indirect immunofluorescence assay, is prone to false-p...
journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
doi:10.1002/(sici)1098-2825(1998)12:1<54::aid-jcla9>3.
更新日期:1998-01-01 00:00:00
abstract:BACKGROUND:This study aimed to evaluate the association of circular RNA La-related RNA-binding protein 4 (circ-LARP4) with clinical features and prognosis in osteosarcoma patients, and further explore its effect on chemosensitivity in osteosarcoma cells. METHODS:Seventy-two osteosarcoma patients with Enneking stage II...
journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
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更新日期:2020-02-01 00:00:00
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journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
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更新日期:2019-05-01 00:00:00
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journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
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更新日期:2004-01-01 00:00:00
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journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
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journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
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更新日期:2016-11-01 00:00:00
abstract::T-lymphocyte function was investigated in a group of eight patients with polycythemia vera (PV) and 13 normals. Responsiveness of T-cells to stimulation by mitogen was significantly impaired in the PV patients (p = .02). Analysis of immune cell subpopulation composition by assessment of cell surface phenotype did not ...
journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
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更新日期:1989-01-01 00:00:00