Comparison of a New and Rapid Method: Brucella Coombs Gel Test With Other Diagnostic Tests.

abstract：:The human insulin domains, signal peptide, B-chain, C-peptide, and A-chain, were highly expressed in Escherichia coli as recombinant proteins N-terminally fused to glutathione-S-transferase and a histidine-hexapeptide. The recombinant proteins were purified from insoluble cell fraction by affinity chromatography using...

journal_title：Journal of clinical laboratory analysis

authors： Walter M,Berg H,Northemann W

更新日期：1994-01-01 00:00:00

abstract：BACKGROUND:For the forensic aim, a sensitive and specific method using headspace gas chromatography coupled with mass spectrometry (GC/MS) has been developed for the quantitative determination of ethanol in blood using n-propanol as internal standard. GC was performed in isothermal mode with a GC run-time of 5.0 min. ...

journal_title：Journal of clinical laboratory analysis

authors： Xiao HT,He L,Tong RS,Yu JY,Chen L,Zou J,Li JQ,Bian Y,Zhang Y

更新日期：2014-09-01 00:00:00

abstract：BACKGROUND:Technical innovation of autoimmune blistering dermatoses (ABDs) diagnosis aimed at multiplex approach. Two multiparametric ELISA tests are commercially available for ABDs serology. The aim was to compare diagnostic accuracy of multiparametric and monospecific ELISAs and to examine the diagnostic value/agreem...

journal_title：Journal of clinical laboratory analysis

authors： Gornowicz-Porowska J,Seraszek-Jaros A,Bowszyc-Dmochowska M,Bartkiewicz P,Kaczmarek E,Dmochowski M

更新日期：2018-05-01 00:00:00

abstract：BACKGROUND:This study was performed to compare the clinical efficacies of anterior cruciate ligament (ACL) reconstruction with autologous ligament grafting at different time points. METHODS:Eighty-five patients with ACL were categorized into two groups: Group A (GA, n = 45), who underwent early-stage (≤3 weeks) surger...

journal_title：Journal of clinical laboratory analysis

authors： Hu B,Gao F,Li C,Zhang B,An M,Lu M,Liu Y,Liu Y

更新日期：2020-08-26 00:00:00

abstract：:Enteroviruses (EVs) are the most commonly identified cause of aseptic meningitis. Rapid detection and characterization of EV meningitis is essential in making decisions for patient management and treatment. A total of 52 cases of acute aseptic meningitis that occurred from March 2003 to April 2005 were investigated fo...

journal_title：Journal of clinical laboratory analysis

authors： Dumaidi K,Frantzidou F,Papa A,Diza E,Antoniadis A

更新日期：2006-01-01 00:00:00

abstract：:It has been proposed that sample storage may have some influence on the parameters of oxidative stress status (OSS) in biological fluids. We measured four important OSS parameters in plasma of 23 healthy subjects and repeated the measurements in the same samples kept at -70 degrees C after different time intervals. Hy...

journal_title：Journal of clinical laboratory analysis

authors： Firuzi O,Mladenka P,Riccieri V,Spadaro A,Petrucci R,Marrosu G,Saso L

更新日期：2006-01-01 00:00:00

abstract：BACKGROUND:This study aimed to investigate the relationship between HbA1c and RBC aggregation in type 2 diabetes mellitus (T2DM) patients by analysis of data from routine clinical tests and from in vitro experiments. METHODS:A total of 2,111 inpatients with type 2 diabetes were selected and among them, 364 patients (G...

journal_title：Journal of clinical laboratory analysis

authors： Li Q,Li L,Li Y

更新日期：2015-09-01 00:00:00

abstract：BACKGROUND:Risperidone (RIS) is a widely used atypical antipsychotic drug. We developed and validated a sensitive and accurate LC-MS/MS method, which requires a small-volume of plasma and small-volume injection for measurement of RIS levels in ASD pediatric patients. We also investigated the relationship between RIS le...

journal_title：Journal of clinical laboratory analysis

authors： Vanwong N,Prommas S,Puangpetch A,Hongkaew Y,Nuntamool N,Nakorn CN,Ngamsamut N,Limsila P,Sukasem C

更新日期：2016-11-01 00:00:00

abstract：:The effects of TPA (12-0-tetradecanoylphorbol-13-acetate) and RA (retinoic acid) were investigated on the cell lines HL60 (acute promyelocytic leukemia) and K562 (erythroleukemia) and on cells from patients with several kinds of leukemia. There were 14 cases of acute lymphocytic leukemia (ALL), 2 cases of chronic lymp...

journal_title：Journal of clinical laboratory analysis

authors： Marques-Silva VM,De Souza MH,Teixeira MC,Arcuri RA,Rumjanek VM

更新日期：1990-01-01 00:00:00

abstract：BACKGROUND:Reference intervals are critical for interpreting test results of a clinical laboratory. The aim of this study was to establish local reference intervals of complete blood count for healthy preschoolers in China. METHODS:Three thousand eight hundred and twenty-one blood specimens from children aged 4 months...

journal_title：Journal of clinical laboratory analysis

authors： Wang GC,Li N,Niu C,Ma WB,Wang ZL,Guo H,Han LH

更新日期：2017-09-01 00:00:00

abstract：BACKGROUND:Liver biopsy is recommended in the majority of patients with chronic viral hepatitis for fibrosis evaluation. Because of the potential risks of liver biopsy, many studies related to non-invasive biomarkers of hepatic fibrosis have been performed. We aimed to assess the diagnostic value of serum biglycan as a...

journal_title：Journal of clinical laboratory analysis

authors： Ciftciler R,Ozenirler S,Yucel AA,Cengiz M,Erkan G,Buyukdemirci E,Sönmez C,Esendaglı GY

更新日期：2017-09-01 00:00:00

abstract：BACKGROUND:Although a diagnosis of infectious diseases is essential for timely treatment, the performance of diagnostic tests has been hardly evaluated due to variable results that are influenced by multiple factors in different conditions. In the present study, the performance of the Alinity i system, which is a newly...

journal_title：Journal of clinical laboratory analysis

authors： Nam M,Song DY,Song SH,Roh EY,Shin S,Park KU,Song EY

更新日期：2020-12-07 00:00:00

abstract：BACKGROUND:MYBPC3 mutations have been described in dilated cardiomyopathy (DCM) and hypertrophic cardiomyopathy (HCM). A mutation, c.3373G>A, has been reported to cause autosomal recessive form of HCM. Here, we report that this mutation can cause autosomal dominant form of DCM. METHODS:Next-generation sequencing using...

journal_title：Journal of clinical laboratory analysis

authors： Mahdieh N,Hosseini Moghaddam M,Motavaf M,Rabbani A,Soveizi M,Maleki M,Rabbani B,Alizadeh-Asl A

更新日期：2018-07-01 00:00:00

abstract：BACKGROUND:The GABA-A receptor signaling pathway regulates proliferation, differentiation, apoptosis, and responses to overt DNA damage during embryonic development. METHODS:To analyze the gene expression after intracytoplasmic sperm injection (ICSI) and in in vivo mouse embryos, the global pattern of gene expression ...

journal_title：Journal of clinical laboratory analysis

authors： Zhang Z,Wang T,Huang J,Huang Y,Zhang Q

更新日期：2020-09-20 00:00:00

abstract：BACKGROUND:Inflammation plays an important role in the development and progression of CRC. The members of inflammatory biomarkers, preoperative NLR and PLR, have been proved by numerous studies to be promising prognostic biomarkers for CRC. However, the diagnostic value of the two biomarkers in CRC remains unknown, and...

journal_title：Journal of clinical laboratory analysis

authors： Peng HX,Yang L,He BS,Pan YQ,Ying HQ,Sun HL,Lin K,Hu XX,Xu T,Wang SK

更新日期：2017-09-01 00:00:00

abstract：:In order to analyze the epitope structure of carcinoembryonic antigen (CEA) and the idiotype network system, seven anti-idiotypic monoclonal antibodies (anti-Id MoAbs) were generated from a BALB/c mouse immunized with anti-CEA MoAb P1-356, which recognized a synthetic peptide P1 of CEA, domain I. These MoAbs were divi...

journal_title：Journal of clinical laboratory analysis

authors： Tsujisaki M,Masuya J,Okada Y,Jinnohara T,Sasaki S,Yachi A,Imai K

更新日期：2002-01-01 00:00:00

abstract：OBJECTIVE:The coronavirus disease 2019 (COVID-19) has rapidly developed into a pandemic. Increased levels of ferritin due to cytokine storm and secondary hemophagocytic lymphohistiocytosis were found in severe COVID-19 patients. Therefore, the aim of this study was to determine the role of ferritin in COVID-19. METHOD...

journal_title：Journal of clinical laboratory analysis

authors： Cheng L,Li H,Li L,Liu C,Yan S,Chen H,Li Y

更新日期：2020-10-01 00:00:00

abstract：BACKGROUND:Although it is widely acknowledged that physical activity confers several health benefits, it remains uncertain whether strenuous and physically demanding exercise might determine biological effects that might turn to be ultimately unfavorable for health. Copeptin and midregion proadrenomedullin (MR-proADM) ...

journal_title：Journal of clinical laboratory analysis

authors： Lippi G,Schena F,Salvagno GL,Sanchis-Gomar F,Guidi GC

更新日期：2015-01-01 00:00:00

abstract：BACKGROUND:Although recent studies have indicated that gut microbiome dysbiosis was significantly associated with the onset of type 2 diabetes mellitus (T2DM), information on the role of blood microbiome in T2DM development is scarce. METHODS:Fifty incident T2DM cases and 100 matched non-T2DM controls were selected fr...

journal_title：Journal of clinical laboratory analysis

authors： Qiu J,Zhou H,Jing Y,Dong C

更新日期：2019-05-01 00:00:00

abstract：:Mycobacterium avium is an important intracellular pathogen, particularly in AIDS patients. It also shows the second frequency among nontuberculous mycobacteria infections in Korea. Point mutations of domain V region of the 23S rRNA gene has been known to confer clarithromycin resistance to M. avium. In order to isolat...

journal_title：Journal of clinical laboratory analysis

authors： Lee SH,Park YK,Kim CK,Kim HJ

更新日期：2011-01-01 00:00:00

abstract：:The experimental and clinical effectiveness of nasal salmon calcitonin (SCT) for treatment of osteoporosis in humans has been well established, but none is known yet about the pharmacokinetic property in relation to therapeutic efficacy, especially when used in a therapeutic dose range. This preclinical study was desi...

journal_title：Journal of clinical laboratory analysis

authors： Nakamuta H,Kohno T,Ichikawa M,Hoshino T,Watabe K,Koida M

更新日期：1997-01-01 00:00:00

abstract：BACKGROUND:Delta neutrophil index (DNI) is a new inflammatory marker and the present study aimed to evaluate the predictive value of the DNI for the presence of a perforation in elderly with acute appendicitis. METHODS:This retrospective observational study was conducted on 108 consecutive elderly patients (≥65 years ...

journal_title：Journal of clinical laboratory analysis

authors： Shin DH,Cho YS,Kim YS,Ahn HC,Oh YT,Park SO,Won MH,Cho JH,Kim YM,Seo JY,Lee YH

更新日期：2018-01-01 00:00:00

abstract：:Our objectives in this study were to compare results obtained by an enzyme immunoassay (EIA) for F-actin antibody (FAA) immunoglobulin G (IgG) to those determined by an indirect fluorescent antibody (IFA) assay for smooth muscle antibody (SMA) IgG, and to determine the prevalence of FAA in patient sera having serologi...

journal_title：Journal of clinical laboratory analysis

authors： Jaskowski TD,Konnick EQ,Ashwood ER,Litwin CM,Hill HR

更新日期：2007-01-01 00:00:00

abstract：:Hypoxia and ischemia play important roles in the onset and progression of glaucoma. Insulin-like growth factors (IGF) are important neurotrophic agents that respond to hypoxia-ischemia. In this study, we enrolled 60 primary open angle glaucoma (POAG) patients and 104 healthy volunteers from the China Medical College H...

journal_title：Journal of clinical laboratory analysis

authors： Tsai FJ,Lin HJ,Chen WC,Chen HY,Fan SS

更新日期：2003-01-01 00:00:00

abstract：:A simple, rapid assay for the pancreatic isoenzyme of human serum amylase was developed. The assay utilized an immunoabsorbent prepared by coating latex beads with a monoclonal antibody specific for pancreatic amylase. Treatment of patient serum with immunoabsorbent removed pancreatic amylase, and measurement of resid...

journal_title：Journal of clinical laboratory analysis

authors： Landt M,Hortin GL,Smith CH,Pashos G,Vaidya HC,Rosenblum JL

更新日期：1994-01-01 00:00:00

abstract：BACKGROUND:Essential hypertension (EH) is an inflammatory disease, and endothelial dysfunction induced by chronic inflammation is one of the pathogeneses of EH. The expression of some inflammatory mediators may be regulated by the interaction of circular RNAs (circRNAs) and microRNAs (miRNAs). METHODS:An Agilent human...

journal_title：Journal of clinical laboratory analysis

authors： He X,Bao X,Tao Z,Sun J,Zheng S,Zhong F,Zhang L

更新日期：2020-11-24 00:00:00

abstract：:Adenosine deaminase (ADA) exists as two isoenzymes, ADA(1) and ADA(2). It appears that the ADA(2) isoenzyme originates mainly from monocytes and macrophages. In tuberculous pleural effusions most of the ADA activity consists of ADA(2). The aim of this prospective study was to analyse ADA isoenzymes in the CSF of patie...

journal_title：Journal of clinical laboratory analysis

authors： Schutte CM,Ungerer JP,du Plessis H,van der Meyden CH

更新日期：2001-01-01 00:00:00

abstract：OBJECTIVE:This present study aimed to investigate the correlation of circular RNA SMARCA5 (circ-SMARCA5) with clinicopathological features and overall survival (OS), and the effect of circ-SMARCA5 on cell proliferation and chemotherapy sensitivity to cisplatin/gemcitabine in intrahepatic cholangiocarcinoma (ICC). METH...

journal_title：Journal of clinical laboratory analysis

authors： Lu Q,Fang T

更新日期：2020-04-01 00:00:00

abstract：:Quantitative determination of albumin (ALB) in human urine is important to assess kidney functions in a variety of diseases. Recently, Siemens released an improved Microalbumin assay (microALB_2) to measure urinary ALB on the automated, random access ADVIA 1650/1800, ADVIA 2400, and ADVIA 1200 Chemistry Systems. We ev...

journal_title：Journal of clinical laboratory analysis

authors： Datta P,Dasgupta A

更新日期：2009-01-01 00:00:00

abstract：:We studied antibody production in serial serum samples from patients with acute Epstein-Barr virus (EBV) and cytomegalovirus (CMV) infections. Sera were analyzed both by enzyme-linked immunosorbent assay (ELISA) using a synthetic peptide (P62) derived from the glycine-alanine repeating region of the Epstein-Barr nucle...

journal_title：Journal of clinical laboratory analysis

authors： Rhodes G,Smith RS,Rubin RE,Vaughan J,Horwitz CA

更新日期：1990-01-01 00:00:00