Parameters of oxidative stress status in healthy subjects: their correlations and stability after sample collection.

abstract：BACKGROUND:Delta neutrophil index (DNI) is a new inflammatory marker and the present study aimed to evaluate the predictive value of the DNI for the presence of a perforation in elderly with acute appendicitis. METHODS:This retrospective observational study was conducted on 108 consecutive elderly patients (≥65 years ...

journal_title：Journal of clinical laboratory analysis

authors： Shin DH,Cho YS,Kim YS,Ahn HC,Oh YT,Park SO,Won MH,Cho JH,Kim YM,Seo JY,Lee YH

更新日期：2018-01-01 00:00:00

abstract：:A radioactive polymerase chain reaction (PCR) method has been developed for the relative quantification of the human alpha-2 chain of type I collagen [hu alpha-2(I)] in cells. cDNAs generated by reverse transcription from the total pool of cytoplasmic RNA serve as a template for polymerase chain reaction amplification...

journal_title：Journal of clinical laboratory analysis

authors： Yu YL,Golden JA,Migchielsen AA,Goetzl EJ,Turck CW

更新日期：1991-01-01 00:00:00

abstract：BACKGROUND:This study was performed to investigate the epidemiology of enterovirus (EV) infections in children at a single hospital during recent 5 years. This study was preformed from December 2008 to November 2013. METHODS:We subjected 1,067 cerebrospinal fluid (CSF) specimens from 1,053 patients and 678 stool speci...

journal_title：Journal of clinical laboratory analysis

authors： Jeon JS,Kim JK,Yu J,Kim JW

更新日期：2016-03-01 00:00:00

abstract：BACKGROUND:The performances of the QMS(®) Teicoplanin immunoassay recently developed on Cobas(®) 6000/8000 systems were evaluated and compared to a fluorescence polarization immunoassay (FPIA) [Teicoplanin Innofluor(®) Assay (Thermo Fisher Scientific, Indianapolis, IN)] on FLX analyzer (Abbott Laboratories, Abbott Park...

journal_title：Journal of clinical laboratory analysis

authors： Dailly E,Fraissinet F,Deslandes G,Bouquié R,Jolliet P

更新日期：2013-03-01 00:00:00

abstract：BACKGROUND:DRI cotinine assay is suitable only for screening for cotinine in urine specimens. We studied the reliability of DRI cotinine semiquantitative values by comparing them with the cotinine concentration obtained with a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method. METHODS:Semiquantitative c...

journal_title：Journal of clinical laboratory analysis

authors： Dixon RB,Dasgupta A

更新日期：2016-11-01 00:00:00

abstract：BACKGROUND:Automated cell counters have become more and more sophisticated with passing years. The numerical and graphic data both provide useful clues for suspecting a diagnosis especially when the workload is very high. AIM:We present our experience of useful information provided by graphic displays of an automated ...

journal_title：Journal of clinical laboratory analysis

authors： Gupta M,Chauhan K,Singhvi T,Kumari M,Grover RK

更新日期：2018-06-01 00:00:00

abstract：:Rats exposed to constant light develop polycystic ovarian (PCO) disease with persistent estrus, representing an estrogen-dominant condition. Herein, we report that fluctuations seen in the vaginal microflora in cyclic rats were not observed in PCO rats with persistent estrus. The vaginal-cervical mucosa of PCO rats sh...

journal_title：Journal of clinical laboratory analysis

authors： Singh KB,Mahajan DK,Tewari RP

更新日期：1996-01-01 00:00:00

abstract：AIMS:Essential hypertension (EH) is a high prevalence disease facing a public health challenge. People were little known about the genetics of diagnosing the cause of EH. Circular RNAs that have a continuous cycle of covalent closure, without affected by RNA exonuclease, and are more stable and hard to degrade may invo...

journal_title：Journal of clinical laboratory analysis

authors： Bao X,He X,Zheng S,Sun J,Luo Y,Tan R,Zhao J,Zhong F,Zhang L

更新日期：2019-05-01 00:00:00

abstract：OBJECTIVE:This present study aimed to investigate the correlation of circular RNA SMARCA5 (circ-SMARCA5) with clinicopathological features and overall survival (OS), and the effect of circ-SMARCA5 on cell proliferation and chemotherapy sensitivity to cisplatin/gemcitabine in intrahepatic cholangiocarcinoma (ICC). METH...

journal_title：Journal of clinical laboratory analysis

authors： Lu Q,Fang T

更新日期：2020-04-01 00:00:00

abstract：:We report nine donations with ABO inconsistency in reverse typing caused by partly or entirely missing antibodies. A and B antigens and antibodies were examined by serological blood typing, and ABO deoxyribonucleic acid (DNA) analyses were performed by sequence-specific priming and sequencing. A B101 allele was demons...

journal_title：Journal of clinical laboratory analysis

authors： Deng ZH,Zeng JQ,Yu Q,Su YQ,Liang YL,Lu L,Zhu WG,Yang BC

更新日期：2007-01-01 00:00:00

abstract：BACKGROUND:Essential trace elements are vital to human health. In this study, our aim was to establish reference intervals of and to evaluate relationships among Ca, Fe, Cu, Mg, and Zn for children. METHODS:We collected blood samples of 3210 children aged 0-14 years from Lu'an, China, and concentrations of the above e...

journal_title：Journal of clinical laboratory analysis

authors： Zhai R,Zhang M,Liu J,Guang H,Li B,Chen D,Zhang S

更新日期：2017-03-01 00:00:00

abstract：:Gross cystic breast disease (GCBD) is the most common benign disease of the human female breast, and patients with GCBD have an increased risk of breast cancer. The aim of this study was to evaluate the distribution inside apocrine cells and in breast cyst fluids aspirated from gross cysts of prostate-specific antigen...

journal_title：Journal of clinical laboratory analysis

authors： Malatesta M,Mannello F,Sebastiani M,Gazzanelli G

更新日期：2001-01-01 00:00:00

abstract：:Using a specific serum anti-soluble T lymphocytes receptor for sheep erythrocytes (E) and SDS-PAGE, we detected radioactive bands of molecular weight 58,000 in immunoprecipitates of supernatant of heated human lymphocytes (SHL), in the supernatant of PHA stimulated lymphocyte cultures (SLC), normal human serum (NHS), ...

journal_title：Journal of clinical laboratory analysis

authors： Itano EN,Ono MA,Sumigawa M,Longo IM,Moura NC,Mendes NF

更新日期：1991-01-01 00:00:00

abstract：:We described an automated enzymatic assay for conjugated bilirubin (Bc) in serum using the Iatro D-Bil kit, with bilirubin oxidase (EC 1.3.3.5 BOD) from Myrothecium species. The specificity of the enzyme in the Iatro D-Bil kit was examined by analyzing unconjugated bilirubin (Bu), delta bilirubin (Bdelta), and Bc with...

journal_title：Journal of clinical laboratory analysis

authors： Kimura S,Iyama S,Yamaguchi Y,Hayashi S,Yanagihara T

更新日期：1999-01-01 00:00:00

abstract：:Novel molecular approaches have recently become available allowing improved major histocompatibility complex (MHC) matching of potential allogeneic bone marrow donors and recipients. Current cellular and serological assays are hindered by aberrant cell populations and limited reagents which only detect an individuals'...

journal_title：Journal of clinical laboratory analysis

authors： Rubocki RJ,Wisecarver JL,Hook DD,Cox SM,Beisel KW

更新日期：1992-01-01 00:00:00

abstract：:Ninety semen samples from male patients were studied. They were classified into 4 different groups; normospermic fertile, oligospermia, asthenospermia, and oligoasthenospermia. In addition to routine semen analysis, the hypo-osmotic swelling test was done for all these samples, measuring total protein and specific gra...

journal_title：Journal of clinical laboratory analysis

authors： al-Mogazy HM,al-Khadra S,Kenawy G,al-Zeftawy A,Mostafa T,Attia A,Attia AM

更新日期：1993-01-01 00:00:00

abstract：:Recently, Siemens Diagnostics released a new calcium assay (CA_2) based on complex formation of calcium with Arsenazo III dye for use on the three automated, random access ADVIA Chemistry analyzers (1650, 2400, and 1200). We evaluated this method for analytical performance as well as potential interference from gadoli...

journal_title：Journal of clinical laboratory analysis

authors： Datta P,Dasgupta A

更新日期：2009-01-01 00:00:00

abstract：:Hematological parameters are affected by different factors that include age, sex, smoking, ethnicity, and environmental altitude. It has been justified that each population must establish its own normal reference intervals to be used in clinical assessments and interpretations. Hematological reference intervals for ad...

journal_title：Journal of clinical laboratory analysis

authors： Sirdah MM,Tarazi IS,El Jeadi H,Al Haddad RM

更新日期：2008-01-01 00:00:00

abstract：:The levels of several soluble cytokine receptors in body fluids of healthy individuals change with age. Clinical application of the measurement of the serum soluble interleukin-1 receptor type I (sIL-1RI) level depends critically on the samples used as the controls. At present, there is no information regarding the le...

journal_title：Journal of clinical laboratory analysis

authors： Okamoto Y,Tanaka M,Miyahara N,Tanabe M,Gotoh Y,Nagai T,Fujita N,Fukui T,Masuzawa T

更新日期：2009-01-01 00:00:00

abstract：BACKGROUND:The aim of this study was to investigate the differential plasma levels of lipocalin 2 (LCN2) and its complex with MMP-9 (where MMP is matrix metalloproteinase) before and after antibiotic treatment in hospitalized adult patients with community-acquired pneumonia (CAP). METHOD:Plasma LCN2 and LCN2/MMP-9 com...

journal_title：Journal of clinical laboratory analysis

authors： Yeh YH,Chang JL,Hsiao PC,Tsao SM,Lin CH,Kao SJ,Chou MC,Yang SF,Chien MH

更新日期：2013-07-01 00:00:00

abstract：:An automated measurement of total and free hydroxyproline in serum or urine is presented that uses flow injection analysis. After exclusion of nonspecific substances, hydroxyproline was oxidized by chloramine-T and L-cysteine with Ehrlich's reagent. The linearity obtained was from 3.8 mumole/L to 1.22 mmole/L with goo...

journal_title：Journal of clinical laboratory analysis

authors： Uji Y,Karmen A,Okabe H,Hata K,Miura M,Ozaki K,Minamizaki M,Shibata T,Inayama S

更新日期：1994-01-01 00:00:00

abstract：BACKGROUND:Renal failure is a severe complication of symptomatic myeloma, related to higher mortality. Recovery from dialysis dependence can lead to enormous survival benefits. We investigated the effect factors for probability of dialysis independence. METHODS:Retrospective data on 45 newly diagnosed MM (NDMM) patien...

journal_title：Journal of clinical laboratory analysis

authors： Song J,Jiang F,Liu H,Ding K,Ren Y,Li L,Wang G,Shao Z,Fu R

更新日期：2020-02-01 00:00:00

abstract：BACKGROUND:We evaluated the new body fluid module on Sysmex UF1000-i (UF1000i-BF) for analysis of white blood cell (WBC) and red blood cell (RBC) in cerebrospinal fluid (CSF). METHODS:WBC and RBC counting were compared between UF1000i-BF and Fuchs-Rosenthal counting chamber in 67 CSF samples. This study also included ...

journal_title：Journal of clinical laboratory analysis

authors： Buoro S,Apassiti Esposito S,Alessio M,Crippa A,Ottomano C,Lippi G

更新日期：2016-09-01 00:00:00

abstract：BACKGROUND:A convenient biosensor for real-time measurement of biomarkers for in-field psychophysiological stress research and military operations is desirable. We evaluated a hand-held device for measuring salivary amylase as a stress marker in medical technicians undergoing combat casualty care training using two dif...

journal_title：Journal of clinical laboratory analysis

authors： Peng HT,Savage E,Vartanian O,Smith S,Rhind SG,Tenn C,Bjamason S

更新日期：2016-05-01 00:00:00

abstract：:Clinicians recognize and compensate for limitations in estimating the glomerular filtration rate (GFR) using serum creatinine (sCr) measurements by the use of timed collections and mathematical manipulations of sCr. These limitations stem from that fact that sCr is affected by nonrenal influences, including muscle mas...

journal_title：Journal of clinical laboratory analysis

authors： Massey D

更新日期：2004-01-01 00:00:00

abstract：:In order to establish a rapid staining method sensitive enough to stain the protein bands on cellulose acetate membrane and suitable for the analysis of urinary proteins in healthy subjects, we conducted a detailed examination of Kovarik's method designed for the analysis of proteins in cerebrospinal fluid in detail. ...

journal_title：Journal of clinical laboratory analysis

authors： Hiratsuka N,Shiba K,Shinomura K,Hosaki S

更新日期：1996-01-01 00:00:00

abstract：BACKGROUND:Non-acrocentric satellited chromosomes mostly result from familial balanced insertions or translocations with p12 or p13 of any acrocentric. Although all non-acrocentrics have been involved, only 12 instances of chromosome 6 involvement are known. CASE PRESENTATION:A female infant exhibited clinical feature...

journal_title：Journal of clinical laboratory analysis

authors： Domínguez MG,Rivera H,Dávalos-Pulido RM,Dávalos-Rodríguez IP

更新日期：2020-08-01 00:00:00

abstract：BACKGROUND:The human epididymis protein 4 (HE4) may have high specificity in the detection of malignant diseases, making the development of an immunoassay for HE4 essential. METHODS:In our study, a fusion gene was constructed encoded with the HE4 protein. This protein was then produced in the bacterial cells (Escheric...

journal_title：Journal of clinical laboratory analysis

authors： Zhou L,Lv Z,Shao J,Xu Y,Luo X,Zhang Y,Hu Y,Zhang W,Luo S,Fang J,Wang Y,Duan C,Huang R

更新日期：2016-09-01 00:00:00

abstract：:The experimental and clinical effectiveness of nasal salmon calcitonin (SCT) for treatment of osteoporosis in humans has been well established, but none is known yet about the pharmacokinetic property in relation to therapeutic efficacy, especially when used in a therapeutic dose range. This preclinical study was desi...

journal_title：Journal of clinical laboratory analysis

authors： Nakamuta H,Kohno T,Ichikawa M,Hoshino T,Watabe K,Koida M

更新日期：1997-01-01 00:00:00

abstract：:Polyfunctional hydrophilic microspheres of 125-nm diameter can be produced by copolymerization of acrylic monomers. Purified c-reactive protein (CRP) was covalently bound to these new micropheres, and the conjugate obtained was used as reagent in a microparticle-enhanced nephelometric immunoassay for human CRP. This a...

journal_title：Journal of clinical laboratory analysis

authors： Montagne P,Laroche P,Cuillière ML,Varcin P,Pau B,Duheille J

更新日期：1992-01-01 00:00:00