Abstract：
:The experimental and clinical effectiveness of nasal salmon calcitonin (SCT) for treatment of osteoporosis in humans has been well established, but none is known yet about the pharmacokinetic property in relation to therapeutic efficacy, especially when used in a therapeutic dose range. This preclinical study was designed to evaluate such a property, first of all in rats, using a novel heterogeneous two-site enzyme immunoassay that has allowed us to evaluate the pharmacokinetic property of parenteral SCT in rats due to the high sensitivity (the detection limit = 2 pg of SCT/ml of plasma). It was found that as early as 10 min after the nasal dosing of 1.25, 5, or 20 U/rat, the SCT immunoactivity became detectable in plasma and thereafter it waned rapidly with time. Hypocalcemia developed in a dose-dependent manner, but with a delay of approximately 20 min from the peak of the immunoactivity and lasted hours. The pharmacokinetic parameters measured for the doses (1.25, 5, and 20 U/rat) were as follows; the AUCs (pg.hr/ml) = 20.8, 89.0, and 189, and the MRTs (min) = 52, 54, and 45, respectively. The results appear to suggest: (1) the unexpected quick transfer of nasal SCT into and from the circulation, (2) a delayed onset of hypocalcemia and possibly its anti-osteopenic action, both of which may last longer, (3) that keeping the plasma SCT above the in vitro anti-osteoclastic level (approximately 1 pM) only for a few hours per 2 days would be enough for inducing the distinct anti-osteopenic effect in rats, and (4) the feasibility of designing the clinical study as to the pharmacokinetics and pharmacodynamics of nasal SCT on humans.
journal_name
J Clin Lab Analjournal_title
Journal of clinical laboratory analysisauthors
Nakamuta H,Kohno T,Ichikawa M,Hoshino T,Watabe K,Koida Mdoi
10.1002/(sici)1098-2825(1997)11:3<129::aid-jcla2>3subject
Has Abstractpub_date
1997-01-01 00:00:00pages
129-31issue
3eissn
0887-8013issn
1098-2825pii
10.1002/(SICI)1098-2825(1997)11:3<129::AID-JCLA2>3journal_volume
11pub_type
杂志文章abstract：BACKGROUND:The trends in usage of tumor markers, including CEA, SCC, NSE, Cyfra21-1, and ProGRP, in Chinese lung cancer patients in the real-world setting are not fully investigated. METHODS:A retrospective descriptive study was conducted using the database of Qilu Hospital of Shandong University, China between Januar...
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abstract：:Enteroviruses (EVs) are the most commonly identified cause of aseptic meningitis. Rapid detection and characterization of EV meningitis is essential in making decisions for patient management and treatment. A total of 52 cases of acute aseptic meningitis that occurred from March 2003 to April 2005 were investigated fo...
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abstract：BACKGROUND:A convenient biosensor for real-time measurement of biomarkers for in-field psychophysiological stress research and military operations is desirable. We evaluated a hand-held device for measuring salivary amylase as a stress marker in medical technicians undergoing combat casualty care training using two dif...
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abstract：BACKGROUND:Screening tests are available to determine immunity to vaccine-preventable diseases, such as mumps and rubella. We aimed to define better assay for detecting immune status of health care personnel to vaccine-preventable diseases. METHODS:Mumps and rubella antibodies of health care personnel at Shimane Unive...
journal_title：Journal of clinical laboratory analysis
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abstract：BACKGROUND:This study was performed to compare the clinical efficacies of anterior cruciate ligament (ACL) reconstruction with autologous ligament grafting at different time points. METHODS:Eighty-five patients with ACL were categorized into two groups: Group A (GA, n = 45), who underwent early-stage (≤3 weeks) surger...
journal_title：Journal of clinical laboratory analysis
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journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
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abstract：BACKGROUND:To study the role of toll-like receptor 4 (TLR4) and MicroRNA-155 (miR-155) in the peripheral blood mononuclear cell (PBMC)-mediated inflammation in coronary slow flow (CSF) and coronary arteriosclerosis. METHODS:Patients were divided into acute coronary syndrome (ACS), stable angina pectoris (SAP), CSF, an...
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abstract：BACKGROUND:The aim of this study was to explain the effects of microRNA-132 in renal cell carcinoma by regulating FOXM1 expression. METHODS:Thirty patients with renal cell carcinoma admitted to our hospital were enrolled, and their adjacent normal tissues and cancer tissues were taken. The expression of microRNA-132 w...
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更新日期：2015-05-01 00:00:00
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journal_title：Journal of clinical laboratory analysis
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更新日期：2016-09-01 00:00:00
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更新日期：2004-01-01 00:00:00
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更新日期：2016-11-01 00:00:00
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更新日期：2019-02-01 00:00:00
abstract：BACKGROUND:Adiponectin, an adipocyte-derived protein, is known to play a key role in the processes leading to atherosclerosis and coronary artery disease (CAD) through its anti-atherogenic, anti-inflammatory, antioxidative, and anti-apoptotic properties. In the current study, we have studied the association of two sing...
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更新日期：2018-09-01 00:00:00
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journal_title：Journal of clinical laboratory analysis
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doi：10.1002/jcla.21723
更新日期：2015-01-01 00:00:00
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journal_title：Journal of clinical laboratory analysis
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更新日期：2008-01-01 00:00:00
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更新日期：2016-03-01 00:00:00
abstract：:The detection of IgA and low-avidity IgG and antibodies in serum is a potentially useful marker of recent infection by a microorganism. We studied the reliability of IgG avidity and presence of IgA for the diagnosis of recent acute infection by rubella virus. Low-avidity IgG (Avy-EIA test) was determined with a modifi...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
