The impact of pre-analytical variations on biochemical analytes stability: A systematic review.

abstract：OBJECTIVES:In this paper, a novel, sensitive and rapid method to simultaneously determine the free and total prostate-specific antigen (fPSA and tPSA) in serum by combining a time-resolved fluoroimmunoassay (TRFIA) and immunomagnetic separation was described. METHODS:The new approach uses magnetic particles as an immo...

journal_title：Journal of clinical laboratory analysis

authors： Zhou B,Zhang J,Lv Z,Fan J,Zhang Y,Huang B

更新日期：2017-11-01 00:00:00

abstract：BACKGROUNDS:Shortening of primary cilia in kidney epithelial cells is associated with kidney injury and involved with the induced level of α-tubulin in urine. Therefore, rapid detection and quantification of α-tubulin in the urine samples could be used to the preliminary diagnosis of kidney injury. METHODS:Cellulose-b...

journal_title：Journal of clinical laboratory analysis

authors： Choi ES,Al Faruque H,Kim JH,Cho JH,Park KM,Kim E

更新日期：2020-01-01 00:00:00

abstract：:Rats exposed to constant light develop polycystic ovarian (PCO) disease with persistent estrus, representing an estrogen-dominant condition. Herein, we report that fluctuations seen in the vaginal microflora in cyclic rats were not observed in PCO rats with persistent estrus. The vaginal-cervical mucosa of PCO rats sh...

journal_title：Journal of clinical laboratory analysis

authors： Singh KB,Mahajan DK,Tewari RP

更新日期：1996-01-01 00:00:00

abstract：BACKGROUND:This study aimed to determine whether CD44 polymorphisms were correlated with hepatocellular carcinoma (HCC) and to reveal a new potential target for early prediction, prevention, and diagnosis of HCC. METHOD:This study involved 96 cases with chronic hepatitis B (CHB), 96 cases with hepatitis B virus-relate...

journal_title：Journal of clinical laboratory analysis

authors： Deng Y,Chen ZJ,Lan F,He QT,Chen SY,Du YF,Li S,Qin X

更新日期：2019-10-01 00:00:00

abstract：:A more sensitive and simpler immune complex transfer enzyme immunoassay for antithyroglobulin IgG in serum and its use for the measurement of antithyroglobulin IgG in healthy subjects and patients with thyroid diseases are described. Antithyroglobulin IgG in test serum was reacted simultaneously with dinitrophenylthyr...

journal_title：Journal of clinical laboratory analysis

authors： Kohno T,Mitsukawa T,Matsukura S,Tsunetoshi Y,Ishikawa E

更新日期：1989-01-01 00:00:00

abstract：:The aim of this study was to evaluate anti-HEV antibody profiles in urine specimens in comparison to corresponding serum samples to assess the utility of urine as a clinical specimen. Paired serum and urine specimens from 71 hepatitis E patients, 33 non-E hepatitis patients, 63 patients with nonhepatic diseases, and 2...

journal_title：Journal of clinical laboratory analysis

authors： Joshi MS,Walimbe AM,Arankalle VA,Chadha MS,Chitambar SD

更新日期：2002-01-01 00:00:00

abstract：BACKGROUND:After hematopoietic stem cell transplantation (HSCT), early detection of engraftment would be of critical value for clinicians. The aim of this study was to identify faster parameters for engraftment. METHODS:We evaluated blood cell parameters including complete blood count (CBC), differential counts, and v...

journal_title：Journal of clinical laboratory analysis

authors： Yahng SA,Lee JW,Kim Y,Kim M,Oh EJ,Park YJ,Lee JW,Cho B,Han K

更新日期：2014-11-01 00:00:00

abstract：AIM:To evaluate, retrospectively, the frequency of autoantibodies of antiphospholipid syndrome (APLS) in Tunisian patients with primary biliary cirrhosis (PBC). PATIENTS AND METHODS:We analyzed 80 PBC sera and 80 sera from blood donors. ELISA was used to determine the frequency of antibodies against cardiolipin (aCL I...

journal_title：Journal of clinical laboratory analysis

authors： Mankaï A,Manoubi W,Ghozzi M,Melayah S,Sakly W,Ghedira I

更新日期：2015-01-01 00:00:00

abstract：AIM:To investigate the presence of antipituitary antibodies (APA) in the serum of patients undergoing gastroenteropathies (GEP) other than celiac disease (CD). METHODS:APA were analyzed in GEP patients (n = 103), CD patients (n = 94), idiopathic growth hormone (GH) deficiency patients (n = 21), and 98 age- and sex-mat...

journal_title：Journal of clinical laboratory analysis

authors： Aguado R,Fernández S,Estévez OA,Santamaría M,Ortega C

更新日期：2014-01-01 00:00:00

abstract：BACKGROUND:Risperidone (RIS) is a widely used atypical antipsychotic drug. We developed and validated a sensitive and accurate LC-MS/MS method, which requires a small-volume of plasma and small-volume injection for measurement of RIS levels in ASD pediatric patients. We also investigated the relationship between RIS le...

journal_title：Journal of clinical laboratory analysis

authors： Vanwong N,Prommas S,Puangpetch A,Hongkaew Y,Nuntamool N,Nakorn CN,Ngamsamut N,Limsila P,Sukasem C

更新日期：2016-11-01 00:00:00

abstract：BACKGROUND:The aim of this study is to evaluate the clinical significance of cystatin C(CysC) in the newborns who show normal serum creatinine (Cr) and who are in an intensive care unit. METHODS:From July 2009 to May 2010, a total of 106 patients (53 male and 53 female newborns) in a neonatal intensive care unit at Ky...

journal_title：Journal of clinical laboratory analysis

authors： Cho SY,Hahn WH,Lee HJ,Suh JT,Lee A,Cho BS,Suh JS

更新日期：2012-07-01 00:00:00

abstract：:Recombinant HIV-1 p66 (rp66, a subunit of reverse transcriptase (RT), a heterodimer of p66 and p51) was produced in Escherichia coli in three different ways. First, rp66 was produced as a part of the fusion protein of lacZ protein and HIV-1 pol protein consisting of three components: protease (p10), RT (p51/p66), and ...

journal_title：Journal of clinical laboratory analysis

authors： Hashinaka K,Nishikata I,Hashida S,Adachi A,Oka S,Ishikawa E

更新日期：2000-01-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay (ELISA) for the detection of antitetanus antibodies was developed using aluminum-absorbed tetanus toxoid as the coating antigen. The assay was tested by measuring antitetanus antibody levels in serum obtained from subjects before and after immunization with the aluminum-absorbed te...

journal_title：Journal of clinical laboratory analysis

authors： Thiele GM,Rogers J,Collins M,Yasuda N,Smith D,McDonald TL

更新日期：1990-01-01 00:00:00

abstract：:Recently, Siemens Diagnostics released a new calcium assay (CA_2) based on complex formation of calcium with Arsenazo III dye for use on the three automated, random access ADVIA Chemistry analyzers (1650, 2400, and 1200). We evaluated this method for analytical performance as well as potential interference from gadoli...

journal_title：Journal of clinical laboratory analysis

authors： Datta P,Dasgupta A

更新日期：2009-01-01 00:00:00

abstract：:N-terminal pro-brain natriuretic peptide (NT-proBNP) and BNP measurement could have a significant role in differentiating dyspnea between cardiac or pulmonary origin in the emergency room. The development of new and different commercial assays for these B-type natriuretic peptides offers the possibility of improving a...

journal_title：Journal of clinical laboratory analysis

authors： Sanz MP,Borque L,Rus A,Vicente B,Ramírez Y,Lasa L

更新日期：2006-01-01 00:00:00

abstract：:Clinicians recognize and compensate for limitations in estimating the glomerular filtration rate (GFR) using serum creatinine (sCr) measurements by the use of timed collections and mathematical manipulations of sCr. These limitations stem from that fact that sCr is affected by nonrenal influences, including muscle mas...

journal_title：Journal of clinical laboratory analysis

authors： Massey D

更新日期：2004-01-01 00:00:00

abstract：BACKGROUND:The trends in usage of tumor markers, including CEA, SCC, NSE, Cyfra21-1, and ProGRP, in Chinese lung cancer patients in the real-world setting are not fully investigated. METHODS:A retrospective descriptive study was conducted using the database of Qilu Hospital of Shandong University, China between Januar...

journal_title：Journal of clinical laboratory analysis

authors： Wang H,Zhang J,Li X,Zhang C,Zheng S,Chi Y,Sheng X,Zhang Y

更新日期：2020-11-01 00:00:00

abstract：:A novel and ultrasensitive noncompetitive enzyme immunoassay (hetero-two-site complex transfer enzyme immunoassay) for alpha-human atrial natriuretic peptide (alpha-hANP) in plasma is described. alpha-hANP was biotinylated using N-hydroxysuccinimidobiotin and trapped onto an anti-alpha-hANP [6-28] IgG-coated polystyre...

journal_title：Journal of clinical laboratory analysis

authors： Hashida S,Ishikawa E

更新日期：1992-01-01 00:00:00

abstract：BACKGROUND:The many techniques used to diagnose the Factor V Leiden (FVL) mutation, the most common hereditary hypercoagulation disorder in Eurasians, and the most frequently requested genetic test reflect the evolving strategies in protein and DNA diagnosis. METHODS:Here, molecular methods to diagnose the FVL mutatio...

journal_title：Journal of clinical laboratory analysis

authors： Oh H,Smith CL

更新日期：2011-01-01 00:00:00

abstract：BACKGROUND:This study was aimed to investigate whether measurement of free testosterone and cortisol in saliva is a reliable alternative to their assessment in serum for monitoring physical fitness in professional athletes. METHODS:We studied 25 members of the soccer team Parma F.C., playing in Italian major football ...

journal_title：Journal of clinical laboratory analysis

authors： Lippi G,Dipalo M,Buonocore R,Gnocchi C,Aloe R,Delsignore R

更新日期：2016-09-01 00:00:00

abstract：BACKGROUND:Alport syndrome (ATS) is a hereditary nephritis with hereditary and clinical heterogeneity; the early clinical symptoms are atypical, which can easily lead to misdiagnosis. The proband, a 6-year-old girl, was found to have microscopic hematuria, proteinuria, and visual impairment at about 5 years old; the re...

journal_title：Journal of clinical laboratory analysis

authors： Zhu Q,Zhou C,Wang J

更新日期：2020-09-06 00:00:00

abstract：BACKGROUND:Automated cell counters have become more and more sophisticated with passing years. The numerical and graphic data both provide useful clues for suspecting a diagnosis especially when the workload is very high. AIM:We present our experience of useful information provided by graphic displays of an automated ...

journal_title：Journal of clinical laboratory analysis

authors： Gupta M,Chauhan K,Singhvi T,Kumari M,Grover RK

更新日期：2018-06-01 00:00:00

abstract：BACKGROUND:Liver biopsy is recommended in the majority of patients with chronic viral hepatitis for fibrosis evaluation. Because of the potential risks of liver biopsy, many studies related to non-invasive biomarkers of hepatic fibrosis have been performed. We aimed to assess the diagnostic value of serum biglycan as a...

journal_title：Journal of clinical laboratory analysis

authors： Ciftciler R,Ozenirler S,Yucel AA,Cengiz M,Erkan G,Buyukdemirci E,Sönmez C,Esendaglı GY

更新日期：2017-09-01 00:00:00

abstract：:Numerous detection methods for Helicobacter Pylori (H. pylori) have been developed with varying degrees of purported diagnostic utility. We have developed a rapid nonradioactive in situ hybridization (ISH) method for H. pylori detection in paraffin-embedded tissue and assessed its relative diagnostic performance by re...

journal_title：Journal of clinical laboratory analysis

authors： Barrett DM,Faigel DO,Metz DC,Montone K,Furth EE

更新日期：1997-01-01 00:00:00

abstract：:As several possible prognostic and therapeutic applications of interleukin-6 are currently under trial, the available methods for its quantification in biological fluids should be evaluated. In this report, the 7TD1 hybridoma bioassay is compared to an enzyme immunoassay for the determination of interleukin-6 in serum...

journal_title：Journal of clinical laboratory analysis

authors： Papadopoulos NG,Dedoussis GV,Baxevanis CN,Papamichail M

更新日期：1995-01-01 00:00:00

abstract：BACKGROUND:A convenient biosensor for real-time measurement of biomarkers for in-field psychophysiological stress research and military operations is desirable. We evaluated a hand-held device for measuring salivary amylase as a stress marker in medical technicians undergoing combat casualty care training using two dif...

journal_title：Journal of clinical laboratory analysis

authors： Peng HT,Savage E,Vartanian O,Smith S,Rhind SG,Tenn C,Bjamason S

更新日期：2016-05-01 00:00:00

abstract：:Quantitative determination of albumin (ALB) in human urine is important to assess kidney functions in a variety of diseases. Recently, Siemens released an improved Microalbumin assay (microALB_2) to measure urinary ALB on the automated, random access ADVIA 1650/1800, ADVIA 2400, and ADVIA 1200 Chemistry Systems. We ev...

journal_title：Journal of clinical laboratory analysis

authors： Datta P,Dasgupta A

更新日期：2009-01-01 00:00:00

abstract：:It is believed that boundary compositions of matrix proteins might play a role in stone formation; however, few proteomic studies concerning matrix proteins in urinary stones have been conducted. In this study, we extracted low molecular weight proteins from calcium oxalate stones and measured their characteristic pat...

journal_title：Journal of clinical laboratory analysis

authors： Chen WC,Lai CC,Tsai Y,Lin WY,Tsai FJ

更新日期：2008-01-01 00:00:00

abstract：:Mycobacterium avium is an important intracellular pathogen, particularly in AIDS patients. It also shows the second frequency among nontuberculous mycobacteria infections in Korea. Point mutations of domain V region of the 23S rRNA gene has been known to confer clarithromycin resistance to M. avium. In order to isolat...

journal_title：Journal of clinical laboratory analysis

authors： Lee SH,Park YK,Kim CK,Kim HJ

更新日期：2011-01-01 00:00:00

abstract：BACKGROUND:The Architect HIV Ag/Ab Combo has excellent performance for HIV screening; however, the false-positive rate (FPR) was high in low HIV prevalence setting. OBJECTIVES:The purpose of this study was to analyze the influence of sample-to-cutoff (s/co) ratios by Architect HIV Ag/Ab Combo with the results of confi...

journal_title：Journal of clinical laboratory analysis

authors： Wang L,Wang JY,Tian XD,Ruan JX,Yu Y,Yan F

更新日期：2019-06-01 00:00:00