The impact of pre-analytical variations on biochemical analytes stability: A systematic review.

abstract：:Congenital disorders of glycosylation are a rapidly growing group of inherited (neuro)metabolic disorders characterized by defects in glycosylation of proteins and lipids. This study discusses an analytical problem in the differentiation between hypoglycosylation and transferrin (Tf) protein variants. Analysis of seru...

journal_title：Journal of clinical laboratory analysis

authors： Marklová E,Albahri Z

更新日期：2009-01-01 00:00:00

abstract：BACKGROUND:Cognitive impairment, significantly reducing processing speed and executive function, is the critical consequence of cerebral small vessel disease (SVD), in which genetic variations have been studied. In this study, we explore the role of SNP43 (G/A) and SNP63 (C/T) polymorphism in the CAPN10 on cognitive im...

journal_title：Journal of clinical laboratory analysis

authors： Wu K,Cai Y

更新日期：2018-11-01 00:00:00

abstract：BACKGROUND:Alport syndrome (ATS) is a hereditary nephritis with hereditary and clinical heterogeneity; the early clinical symptoms are atypical, which can easily lead to misdiagnosis. The proband, a 6-year-old girl, was found to have microscopic hematuria, proteinuria, and visual impairment at about 5 years old; the re...

journal_title：Journal of clinical laboratory analysis

authors： Zhu Q,Zhou C,Wang J

更新日期：2020-09-06 00:00:00

abstract：:A sensitive time-resolved fluorimetric immune-complex-transfer immunoassay for antithyroglobulin IgG in serum is described. Antithyroglobulin IgG in test serum was reacted with dinitrophenyl europium ion-labeled thyroglobulin. The complex formed of antithyroglobulin IgG and dinitrophenyl europium ion-labeled thyroglob...

journal_title：Journal of clinical laboratory analysis

authors： Kohno T,Mitsukawa T,Matsukura S,Ishikawa E

更新日期：1990-01-01 00:00:00

abstract：BACKGROUND:Cardiovascular disease is the most common cause of death worldwide, and the detection of LDL-C contributes to reducing risks. However, the LDL-C is rarely evaluated according to the gold standard method because it is costly and time-consuming. This study aimed to determine the agreement of LDL-C among three ...

journal_title：Journal of clinical laboratory analysis

authors： Sonsok S,Sarakarn P,Sanchaisuriya P

更新日期：2020-10-01 00:00:00

abstract：OBJECTIVE:To explore the role of serum periostin in patients with aneurysmal subarachnoid hemorrhage (aSAH). METHOD:We conducted a retrospective study and 124 aSAH patients treated in Shenzhen People's hospital during March 1st 2015 to December 30th 2016 were included. Baseline information, neurological status and cli...

journal_title：Journal of clinical laboratory analysis

authors： Luo W,Wang H,Hu J

更新日期：2018-06-01 00:00:00

abstract：:We studied a new proteinase K assay method for human serum mitochondrial aspartate aminotransferase. We found that proteinase K showed no inactivation of human mitochondrial aspartate aminotransferase isoenzyme and complete inactivation of cytosolic aspartate aminotransferase. Previous studies have shown that selectiv...

journal_title：Journal of clinical laboratory analysis

authors： Watazu Y,Uji Y,Okabe H,Shirahase Y,Kaneda N,Karmen A

更新日期：1993-01-01 00:00:00

abstract：BACKGROUND:Neurofilaments are the major cytoskeletal components of neurons, and cell injury leads to their release into the surrounding area. The aim of this study was to compare the cerebrospinal fluid (CSF) and serum (S) concentrations of neurofilament light chains (NFLs) and phosphorylated neurofilament heavy chains...

journal_title：Journal of clinical laboratory analysis

authors： Kušnierová P,Zeman D,Hradílek P,Čábal M,Zapletalová O

更新日期：2019-09-01 00:00:00

abstract：BACKGROUND:The aim of this study was to investigate the differential plasma levels of lipocalin 2 (LCN2) and its complex with MMP-9 (where MMP is matrix metalloproteinase) before and after antibiotic treatment in hospitalized adult patients with community-acquired pneumonia (CAP). METHOD:Plasma LCN2 and LCN2/MMP-9 com...

journal_title：Journal of clinical laboratory analysis

authors： Yeh YH,Chang JL,Hsiao PC,Tsao SM,Lin CH,Kao SJ,Chou MC,Yang SF,Chien MH

更新日期：2013-07-01 00:00:00

abstract：:A modification of the existing spectrophotometric kinetic method for the determination of pancuronium bromide (PCBr), based on pooled human serum cholinesterase (ChE, EC 3.1.1.8 acylcholine acylhydrolase) inhibition, was developed. Butyrylthiocholine iodide (concentration 1.667 mmol/L) was used as substrate and determ...

journal_title：Journal of clinical laboratory analysis

authors： Stankov-Jovanovic VP,Nikolic-Mandic SD,Mandic LjM,Mitic VD

更新日期：2007-01-01 00:00:00

abstract：BACKGROUND:This study was performed to investigate the epidemiology of enterovirus (EV) infections in children at a single hospital during recent 5 years. This study was preformed from December 2008 to November 2013. METHODS:We subjected 1,067 cerebrospinal fluid (CSF) specimens from 1,053 patients and 678 stool speci...

journal_title：Journal of clinical laboratory analysis

authors： Jeon JS,Kim JK,Yu J,Kim JW

更新日期：2016-03-01 00:00:00

abstract：:We aimed to evaluate whether a PEG-IAT method, modified for gel technique, could replace the two-stage enzyme method for routine identification of red blood cell (RBC) antibodies.A total of 161 selected patient samples were analyzed with the two-stage enzyme tube technique and the PEG-IAT in gel techniques. In 65 samp...

journal_title：Journal of clinical laboratory analysis

authors： Gustafsson L,Wikman A,Lundahl J

更新日期：2004-01-01 00:00:00

abstract：:Acid phosphatase was purified to electrophoretic homogeneity from human normal lung and spleen and was characterized biochemically and immunologically in comparison with prostate acid phosphatase (PAP). The apparent MW of lung acid phosphatase (LAP) and spleen acid phosphatase (SAP) was 110,000 and 100,000, respective...

journal_title：Journal of clinical laboratory analysis

authors： Lin MF,Li SS,Chu TM,Lee CL

更新日期：1990-01-01 00:00:00

abstract：:Cholesterol in the diet can readily autoxidize and be absorbed and transported in plasma lipoproteins. Cholesterol oxides can also be endogenously produced in tissues via free-radical-induced reactions. Some cholesterol oxides, notably cholestane-3 beta, 5 alpha, 6 beta-triol and 25-hydroxycholesterol, have been shown...

journal_title：Journal of clinical laboratory analysis

authors： Peng SK,Hu B,Morin RJ

更新日期：1991-01-01 00:00:00

abstract：:A simple, rapid assay for the pancreatic isoenzyme of human serum amylase was developed. The assay utilized an immunoabsorbent prepared by coating latex beads with a monoclonal antibody specific for pancreatic amylase. Treatment of patient serum with immunoabsorbent removed pancreatic amylase, and measurement of resid...

journal_title：Journal of clinical laboratory analysis

authors： Landt M,Hortin GL,Smith CH,Pashos G,Vaidya HC,Rosenblum JL

更新日期：1994-01-01 00:00:00

abstract：:Hemoglobin F (HbF) is an effective inhibitor of HbS polymerization. Hydroxyurea (HU) is used to increase HbF synthesis and improve the clinical course of sickle cell disease (SCD) patients. We studied a series of laboratory parameters concerning HbF production and reticulocyte response, and compared data between two g...

journal_title：Journal of clinical laboratory analysis

authors： Borba R,Lima CS,Grotto HZ

更新日期：2003-01-01 00:00:00

abstract：BACKGROUND:Today, the genetic and genomic research entered in a new era of high-throughput genotyping technology. However, mutagenic polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) is still a choice of genotyping method in molecular epidemiological research. It has been extensively used fo...

journal_title：Journal of clinical laboratory analysis

authors： Sharma N,Awasthi S,Phadke SR

更新日期：2016-03-01 00:00:00

abstract：:This study compared the use of conventional urine microscopy, the KOVA system, Neubauer hemacytometry, and the Yellow IRIS for the determination of white blood cell (WBC) and red blood cell (RBC) counts in urine samples. Both KOVA WBC and RBC counts correlated better with the IRIS counts than with conventional microsc...

journal_title：Journal of clinical laboratory analysis

authors： McGinley M,Wong LL,McBride JH,Rodgerson DO

更新日期：1992-01-01 00:00:00

abstract：BACKGROUND:Inflammation plays an important role in the development and progression of CRC. The members of inflammatory biomarkers, preoperative NLR and PLR, have been proved by numerous studies to be promising prognostic biomarkers for CRC. However, the diagnostic value of the two biomarkers in CRC remains unknown, and...

journal_title：Journal of clinical laboratory analysis

authors： Peng HX,Yang L,He BS,Pan YQ,Ying HQ,Sun HL,Lin K,Hu XX,Xu T,Wang SK

更新日期：2017-09-01 00:00:00

abstract：:p21, an important regulator of the cell cycle, acts as a mediator of the growth-suppressing and -promoting functions of p53. We aimed to investigate the association between codon 31 polymorphisms of p21 gene and endometriosis. Women were divided into two groups: endometriosis (n = 102) and nonendometriosis (n = 119). ...

journal_title：Journal of clinical laboratory analysis

authors： Hsieh YY,Tsai FJ,Chang CC,Chen WC,Tsai CH,Tsai HD,Lin CC

更新日期：2001-01-01 00:00:00

abstract：BACKGROUND:To determine the metabolic characteristics of patients with colon cancer (CC) and rectal cancer (RC) using gas chromatography-mass spectrometry (GC-MS)-based metabolomics. METHODS:In this study, serum samples were collected from 22 CC patients and 23 RC patients preoperatively and postoperatively and 45 hea...

journal_title：Journal of clinical laboratory analysis

authors： Wu J,Wu M,Wu Q

更新日期：2020-08-01 00:00:00

abstract：BACKGROUND:The primary test for the laboratory confirmation of measles is immunoglobulin M (IgM) serology. It is therefore important to evaluate new commercial measles IgM immunoassays to ensure high-quality measles diagnostic testing. The purpose of this study was to evaluate the diagnostic performance of LIAISON IgM ...

journal_title：Journal of clinical laboratory analysis

authors： Sampedro A,Rodríguez-Granger J,Gómez C,Lara A,Gutierrez J,Otero A

更新日期：2013-11-01 00:00:00

abstract：:Apoptosis of epithelial hepatocytes plays a pivotal role in acute as well as in chronic liver diseases. The cleavage of cytokeratin-18 (CK-18) by caspases is an early event in the apoptotic process. We therefore sought to investigate serum levels of CK-18 and 20S proteasome in patients with liver cirrhosis, primary gr...

journal_title：Journal of clinical laboratory analysis

authors： Hetz H,Hoetzenecker K,Hacker S,Faybik P,Pollreisz A,Moser B,Roth G,Hoetzenecker W,Lichtenauer M,Klinger M,Krenn CG,Ankersmit HJ

更新日期：2007-01-01 00:00:00

abstract：:During the past few years, interest in xenotransplantation of porcine islets of Langerhans for the future therapy of type I diabetes has increased markedly. Therefore, we established a semiautomated digestion method for isolating islets from the porcine pancreas. However, although the isolation technique was standardi...

journal_title：Journal of clinical laboratory analysis

authors： Heiser A,Ulrichs K,Müller-Ruchholtz W

更新日期：1994-01-01 00:00:00

abstract：BACKGROUND:Previous studies have demonstrated that dysfunctional regulatory T cells (Tregs) may be associated with Graves' disease (GD). In this study, we evaluated four serum Treg-associated miRNAs (miR-210, miR-182, miR-155, and miR-146a) expressions and assessed the potential of serum miRNAs as biomarkers of GD. ME...

journal_title：Journal of clinical laboratory analysis

authors： Zheng L,Zhuang C,Wang X,Ming L

更新日期：2018-02-01 00:00:00

abstract：:By using commercially available ELISA kits, serum IL-6 and TNF-alpha levels in healthy adults, and the levels of various cytokines in patients with primary biliary cirrhosis or chronic viral liver diseases, were investigated. IL-6 levels of healthy subjects were distributed in a wide range, and the distribution patter...

journal_title：Journal of clinical laboratory analysis

authors： Yamashiki M,Kosaka Y,Nishimura A,Watanabe S,Nomoto M,Ichida F

更新日期：1998-01-01 00:00:00

abstract：BACKGROUND:Selection and verification of blood collection tubes is an important preanalytical issue in clinical laboratories. Today, gel tubes are commonly used with many advantages, although they are known to cause interference in immunoassay methods. In this study, we aimed to compare SSTs of two different suppliers ...

journal_title：Journal of clinical laboratory analysis

authors： Ercan M,Fırat Oğuz E,Akbulut ED,Yilmaz M,Turhan T

更新日期：2018-07-01 00:00:00

abstract：:For many years, fluorescence polarization immunoassay (FPIA) on the TDx analyzer has been used for determination of free phenytoin concentration. Recently Abbott Laboratories decided to discontinue the TDx analyzer and related assays on this analyzer. Free phenytoin assay is also available from Roche Diagnostics for a...

journal_title：Journal of clinical laboratory analysis

authors： Dasgupta A,Davis B,Chow L

更新日期：2013-01-01 00:00:00

abstract：:Radioimmunoassay (RIA) that involves purification of the analyte by organic solvent extraction is widely used. Although the extraction RIA is reliable when properly validated, it is time consuming and radioactive, we measure urinary total testosterone with a highly sensitive rapid and accurate time-resolved fluorescen...

journal_title：Journal of clinical laboratory analysis

authors： Bao S,Peng Y,Sheng S,Lin Q

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:Cell-free DNA (cfDNA) in the plasma of patients with both malignant and benign breast lesions was analyzed to determine whether the findings may have diagnostic and prognostic implications and to analyze the association between the levels of cfDNA and prognostic parameters. METHODS:Plasma samples were obtai...

journal_title：Journal of clinical laboratory analysis

authors： Hashad D,Sorour A,Ghazal A,Talaat I

更新日期：2012-11-01 00:00:00