Abstract:
BACKGROUND:Neurofilaments are the major cytoskeletal components of neurons, and cell injury leads to their release into the surrounding area. The aim of this study was to compare the cerebrospinal fluid (CSF) and serum (S) concentrations of neurofilament light chains (NFLs) and phosphorylated neurofilament heavy chains (pNFHs). METHODS:Neurofilament concentrations were measured in CSF and S samples from 172 patients using three enzyme-linked immunosorbent assays. Excel, Stata version 13, MedCal version 17.9.7., and NCSS 2007 software were used for the statistical analysis. RESULTS:There was a statistically significant correlation between the concentrations of CSF NFL and CSF pNFH (rs = 0.748; n = 89; P < 0.001), but Passing-Bablok regression showed systematic deviation between the values obtained using the two assays. This indicates that the assays were not interchangeable. CSF pNFH and S pNFH concentrations showed low correlation. The kappa statistic showed moderate conformity between CSF pNFH and CSF NFL concentrations (κ = 0.556). CONCLUSIONS:The CSF NFL and CSF pNFH assays gave clinically consistent results that reflected the degree of axonal damage, independent of any particular neurological diagnosis. The S pNFH assays had a lower predictive value due to the low correlation coefficient and the kappa index of the CSF pNFH method.
journal_name
J Clin Lab Analjournal_title
Journal of clinical laboratory analysisauthors
Kušnierová P,Zeman D,Hradílek P,Čábal M,Zapletalová Odoi
10.1002/jcla.22948subject
Has Abstractpub_date
2019-09-01 00:00:00pages
e22948issue
7eissn
0887-8013issn
1098-2825journal_volume
33pub_type
杂志文章abstract:BACKGROUND:Circular RNAs (circRNAs) are emerging noncoding RNAs that are involved in many biological processes and diseases. The expression profile of circRNAs in preterm neonates with bronchopulmonary dysplasia (BPD) remains unresolved. METHODS:In BPD infants, peripheral venous blood was drawn and circRNAs were extra...
journal_title:Journal of clinical laboratory analysis
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journal_title:Journal of clinical laboratory analysis
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journal_title:Journal of clinical laboratory analysis
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journal_title:Journal of clinical laboratory analysis
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更新日期:2003-01-01 00:00:00
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journal_title:Journal of clinical laboratory analysis
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journal_title:Journal of clinical laboratory analysis
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doi:
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journal_title:Journal of clinical laboratory analysis
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更新日期:2020-09-01 00:00:00
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