A modification of the kinetic determination of pancuronium bromide based on its inhibitory effect on cholinesterase.

abstract：:Congenital heart disease (CHD) is a common cardiac defect found in infants and children. Despite advances in diagnosis and treatment, our understanding of the causative mechanism and etiology of CHD is limited. To determine the genetic etiology of CHD, we selected 11 consecutive short tandem-repeat polymorphic (STRP) ...

journal_title：Journal of clinical laboratory analysis

authors： Shi YR,Hsieh KS,Wu JY,Lee CC,Tsai CH,Yu MT,Chang JS,Tsai FJ

更新日期：2003-01-01 00:00:00

abstract：:During the past few years, interest in xenotransplantation of porcine islets of Langerhans for the future therapy of type I diabetes has increased markedly. Therefore, we established a semiautomated digestion method for isolating islets from the porcine pancreas. However, although the isolation technique was standardi...

journal_title：Journal of clinical laboratory analysis

authors： Heiser A,Ulrichs K,Müller-Ruchholtz W

更新日期：1994-01-01 00:00:00

abstract：AIM:To investigate the presence of antipituitary antibodies (APA) in the serum of patients undergoing gastroenteropathies (GEP) other than celiac disease (CD). METHODS:APA were analyzed in GEP patients (n = 103), CD patients (n = 94), idiopathic growth hormone (GH) deficiency patients (n = 21), and 98 age- and sex-mat...

journal_title：Journal of clinical laboratory analysis

authors： Aguado R,Fernández S,Estévez OA,Santamaría M,Ortega C

更新日期：2014-01-01 00:00:00

abstract：:Elevated serum chromogranin A (CgA) levels have been detected in patients with prostate cancer who have developed resistance to hormonal therapy. We would like to reexamine these cases by using serial specimens to determine whether such elevated levels are also detectable in prostate cancer patients not undergoing hor...

journal_title：Journal of clinical laboratory analysis

authors： Wu JT,Wu TL,Chang CP,Tsao KC,Sun CF

更新日期：1999-01-01 00:00:00

abstract：:Premortem diagnosis of the DiGeorge syndrome and its partial variants relies on the demonstration of a primary defect in cell-mediated immunity, generally in the setting of an infant with congenital heart disease, hypocalcemia, absence of a thymic shadow, and typical dysmorphic features. Although T-cell enumeration is...

journal_title：Journal of clinical laboratory analysis

authors： Slade HB,Greenwood JH,Beekman RH 3rd,McCoy JP Jr,Hudson JL,Pahwa S,Schwartz SA

更新日期：1989-01-01 00:00:00

abstract：BACKGROUND:Gastrin-17 (G-17) and Helicobacter pylori (H pylori) antibody are widely used in the screening of gastric diseases, especially in gastric cancer. In this study, we aimed to evaluate the value of G-17 and H pylori antibody in gastric disease screening. METHODS:Healthy males and females (1368 and 1212, respec...

journal_title：Journal of clinical laboratory analysis

authors： Liu W,Sun Y,Yuan Y

更新日期：2020-08-30 00:00:00

abstract：BACKGROUND:Today, the genetic and genomic research entered in a new era of high-throughput genotyping technology. However, mutagenic polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) is still a choice of genotyping method in molecular epidemiological research. It has been extensively used fo...

journal_title：Journal of clinical laboratory analysis

authors： Sharma N,Awasthi S,Phadke SR

更新日期：2016-03-01 00:00:00

abstract：BACKGROUND:After hematopoietic stem cell transplantation (HSCT), early detection of engraftment would be of critical value for clinicians. The aim of this study was to identify faster parameters for engraftment. METHODS:We evaluated blood cell parameters including complete blood count (CBC), differential counts, and v...

journal_title：Journal of clinical laboratory analysis

authors： Yahng SA,Lee JW,Kim Y,Kim M,Oh EJ,Park YJ,Lee JW,Cho B,Han K

更新日期：2014-11-01 00:00:00

abstract：BACKGROUND:Oxidative stress is an imbalance between the reactive oxygen species and antioxidant system. In this study, total oxidative stress (TOS) and total antioxidant capacity (TAC) were investigated with a new and practical method in childhood iron-deficiency anemia. METHOD:During the study period 80 children betw...

journal_title：Journal of clinical laboratory analysis

authors： Akça H,Polat A,Koca C

更新日期：2013-05-01 00:00:00

abstract：BACKGROUND:Acute respiratory distress syndrome (ARDS) is a critical condition characterized by bilateral pulmonary infiltrates and severe hypoxemia. This study aimed to evaluate the diagnostic and prognostic values of Club cell protein 16 (CC16) in critical care patients with ARDS. METHODS:In this retrospective observ...

journal_title：Journal of clinical laboratory analysis

authors： Lin J,Zhang W,Wang L,Tian F

更新日期：2018-02-01 00:00:00

abstract：BACKGROUND:Fork head/winged helix transcription factor (Foxp3) plays a pivotal role in regulatory T (Treg) cells. The present study aimed to assess the association of Crohn's disease (CD) with Foxp3 polymorphisms and its colonic expression in Chinese patients. METHODS:The Foxp3 polymorphisms, rs3761547, rs2232365, rs2...

journal_title：Journal of clinical laboratory analysis

authors： Xia S,Zhang D,Zheng S,Wu C,Lin Q,Ying S,Shao X,Jiang Y

更新日期：2019-05-01 00:00:00

abstract：BACKGROUND:The aim of this study is to evaluate the clinical significance of cystatin C(CysC) in the newborns who show normal serum creatinine (Cr) and who are in an intensive care unit. METHODS:From July 2009 to May 2010, a total of 106 patients (53 male and 53 female newborns) in a neonatal intensive care unit at Ky...

journal_title：Journal of clinical laboratory analysis

authors： Cho SY,Hahn WH,Lee HJ,Suh JT,Lee A,Cho BS,Suh JS

更新日期：2012-07-01 00:00:00

abstract：:There is an increasing demand for genetic testing of patients with hypercoagulability. We have developed a multiplex-polymerase chain reaction (PCR) technique that incorporates the state-of-the art of genetic testing for thrombosis mutations (Factor V Leiden [FVL] and G20210A F2 and C46T F12). The sequences are detect...

journal_title：Journal of clinical laboratory analysis

authors： López M,Giraldo P,Alvarez P,Cornudella R,Pocoví M,Martínez A,Fontcuberta J,Soria JM

更新日期：2007-01-01 00:00:00

abstract：BACKGROUND:Cognitive impairment, significantly reducing processing speed and executive function, is the critical consequence of cerebral small vessel disease (SVD), in which genetic variations have been studied. In this study, we explore the role of SNP43 (G/A) and SNP63 (C/T) polymorphism in the CAPN10 on cognitive im...

journal_title：Journal of clinical laboratory analysis

authors： Wu K,Cai Y

更新日期：2018-11-01 00:00:00

abstract：BACKGROUND:The GABA-A receptor signaling pathway regulates proliferation, differentiation, apoptosis, and responses to overt DNA damage during embryonic development. METHODS:To analyze the gene expression after intracytoplasmic sperm injection (ICSI) and in in vivo mouse embryos, the global pattern of gene expression ...

journal_title：Journal of clinical laboratory analysis

authors： Zhang Z,Wang T,Huang J,Huang Y,Zhang Q

更新日期：2020-09-20 00:00:00

abstract：BACKGROUND:This study aimed to investigate the plasma long non-coding RNA (lncRNA) expression profile in knee osteoarthritis (KOA) patients and the value of candidate lncRNAs for predicting KOA risk. METHODS:Plasma was obtained for RNA sequencing (RNA-seq) in eight KOA patients and eight healthy controls (Ctrls). Ten ...

journal_title：Journal of clinical laboratory analysis

authors： Liu X,Li M,Zhang B,Zhang N,Feng Q

更新日期：2020-09-01 00:00:00

abstract：:Two methods for the extraction of RNA of vesicular stomatitis virus (VSV) Indiana1 and New Jersey and their simultaneous amplification by one-step polymerase chain reaction using reverse transcriptase were evaluated. A guanidine-thiocyanate-based RNA extraction (Qiagen RNeasy Mini Kit, Qiagen, Valencia, CA ) followed ...

journal_title：Journal of clinical laboratory analysis

authors： Arshed MJ,Magnuson RJ,Triantis J,Abubakar M,Van Campen H,Salman M

更新日期：2011-01-01 00:00:00

abstract：:The authors compare efficacy of two ELISA assays (one supplied by DIAMEDIX [Delta Biological s.r.l.], and the other by RADIM [RADIM I]) in detecting total anti-H. pylori antibodies, and of two further ELISA methods (one supplied by EUROSPITAL [Helori CTX IgG] and the other by RADIM [RADIM 2]) in identifying anti-CagA ...

journal_title：Journal of clinical laboratory analysis

authors： Basso D,Stefani A,Brigato L,Navaglia F,Greco E,Zambon CF,Piva MG,Toma A,Di Mario F,Plebani M

更新日期：1999-01-01 00:00:00

abstract：:An automated measurement of total and free hydroxyproline in serum or urine is presented that uses flow injection analysis. After exclusion of nonspecific substances, hydroxyproline was oxidized by chloramine-T and L-cysteine with Ehrlich's reagent. The linearity obtained was from 3.8 mumole/L to 1.22 mmole/L with goo...

journal_title：Journal of clinical laboratory analysis

authors： Uji Y,Karmen A,Okabe H,Hata K,Miura M,Ozaki K,Minamizaki M,Shibata T,Inayama S

更新日期：1994-01-01 00:00:00

abstract：OBJECTIVE:To explore the clinical value of serum IgM and IgG to SARS-CoV-2 in COVID-19. METHODS:105 COVID-19 patients were enrolled as the disease group. 197 non-COVID-19 patients served as the control group. Magnetic chemiluminescent immunoassay (MCLIA) was used to detect the IgM and IgG. RESULTS:The peak of positiv...

journal_title：Journal of clinical laboratory analysis

authors： Wu LX,Wang H,Gou D,Fu G,Wang J,Guo BQ

更新日期：2020-11-13 00:00:00

abstract：:The present study was designed to evaluate whether the use of acid citrate dextrose (ACD) Formula A may enhance the survival of platelets during storage, thus allowing the continuance of platelet studies over the period of 2-3 hours usually recommended. For this purpose the effects of time on in vitro platelet respons...

journal_title：Journal of clinical laboratory analysis

authors： Pignatelli P,Pulcinelli FM,Ciatti F,Pesciotti M,Ferroni P,Gazzaniga PP

更新日期：1996-01-01 00:00:00

abstract：:Using a fully automated reticulocyte counter, the roles of the reticulocyte count with maturation in pancytopenia were evaluated. Different groups of pancytopenia including aplastic anemia, infiltrative marrow disorder, hypersplenism, and megaloblastic anemia were recruited. All patients had bone marrow examinations f...

journal_title：Journal of clinical laboratory analysis

authors： Lin CK,Hsu HC,Chau WK,Jiang ML,Chiu CF

更新日期：1993-01-01 00:00:00

abstract：:The presence of high-molecular intestinal ALP (HIALP) overlapping with bone ALP in the alpha(2)beta region has been demonstrated. In this study we evaluated a method of separating HIALP after its conversion into ALP(5) by the action of protease. Serum samples from patients were mixed with protease at a ratio of 5:1 an...

journal_title：Journal of clinical laboratory analysis

authors： Ooi K,Shiraki K,Morishita Y,Nobori T

更新日期：2007-01-01 00:00:00

abstract：BACKGROUND:The primary test for the laboratory confirmation of measles is immunoglobulin M (IgM) serology. It is therefore important to evaluate new commercial measles IgM immunoassays to ensure high-quality measles diagnostic testing. The purpose of this study was to evaluate the diagnostic performance of LIAISON IgM ...

journal_title：Journal of clinical laboratory analysis

authors： Sampedro A,Rodríguez-Granger J,Gómez C,Lara A,Gutierrez J,Otero A

更新日期：2013-11-01 00:00:00

abstract：BACKGROUND:The prevalence of diabetes is increasing in China. Glucose control is very important in diabetic patients. The aim of this study was to compare the accuracy of five glucose meters used in Chinese hospitals with a reference method, in the absence and presence of various factors that may interfere with the met...

journal_title：Journal of clinical laboratory analysis

authors： Lv H,Zhang GJ,Kang XX,Yuan H,Lv YW,Wang WW,Randall R

更新日期：2013-09-01 00:00:00

abstract：OBJECTIVE:Dynamic monitoring of the concentration variation of IgM and IgG in patients with SARS-CoV-2 infections and exploring their diagnostic value for coronavirus disease-19 (COVID-19). METHODS:A total of 15 patients with SARS-CoV-2 infection were enrolled as the COVID-19 group, and 50 patients were enrolled as th...

journal_title：Journal of clinical laboratory analysis

authors： Lijia S,Lihong S,Huabin W,Xiaoping X,Xiaodong L,Yixuan Z,Pin H,Yina X,Xiaoyun S,Junqi W

更新日期：2020-10-01 00:00:00

abstract：BACKGROUNDS:Shortening of primary cilia in kidney epithelial cells is associated with kidney injury and involved with the induced level of α-tubulin in urine. Therefore, rapid detection and quantification of α-tubulin in the urine samples could be used to the preliminary diagnosis of kidney injury. METHODS:Cellulose-b...

journal_title：Journal of clinical laboratory analysis

authors： Choi ES,Al Faruque H,Kim JH,Cho JH,Park KM,Kim E

更新日期：2020-01-01 00:00:00

abstract：OBJECTIVE:The coronavirus disease 2019 (COVID-19) has rapidly developed into a pandemic. Increased levels of ferritin due to cytokine storm and secondary hemophagocytic lymphohistiocytosis were found in severe COVID-19 patients. Therefore, the aim of this study was to determine the role of ferritin in COVID-19. METHOD...

journal_title：Journal of clinical laboratory analysis

authors： Cheng L,Li H,Li L,Liu C,Yan S,Chen H,Li Y

更新日期：2020-10-01 00:00:00

abstract：BACKGROUND:Reference intervals are important for interpretation of clinical laboratory tests. The platelet (PLT) indices such as the mean platelet volume (MPV) and platelet distribution width (PDW) are newer hematological parameters, which have been recently reported as clinically valuable biomarkers. However, there ar...

journal_title：Journal of clinical laboratory analysis

authors： Hong J,Min Z,Bai-shen P,Jie Z,Ming-ting P,Xian-zhang H,Xiao-ke H,Lan-lan W,Xin Z,Wei G,Rui Q,Wen-xiang C,Xin-zhong W,Yue-yun M,Hong S

更新日期：2015-01-01 00:00:00

abstract：:Quantitative determination of albumin (ALB) in human urine is important to assess kidney functions in a variety of diseases. Recently, Siemens released an improved Microalbumin assay (microALB_2) to measure urinary ALB on the automated, random access ADVIA 1650/1800, ADVIA 2400, and ADVIA 1200 Chemistry Systems. We ev...

journal_title：Journal of clinical laboratory analysis

authors： Datta P,Dasgupta A

更新日期：2009-01-01 00:00:00