Serum antibodies anti-H. pylori and anti-CagA: a comparison between four different assays.

abstract：:We studied antibody production in serial serum samples from patients with acute Epstein-Barr virus (EBV) and cytomegalovirus (CMV) infections. Sera were analyzed both by enzyme-linked immunosorbent assay (ELISA) using a synthetic peptide (P62) derived from the glycine-alanine repeating region of the Epstein-Barr nucle...

journal_title：Journal of clinical laboratory analysis

authors： Rhodes G,Smith RS,Rubin RE,Vaughan J,Horwitz CA

更新日期：1990-01-01 00:00:00

abstract：:Our objectives in this study were to compare results obtained by an enzyme immunoassay (EIA) for F-actin antibody (FAA) immunoglobulin G (IgG) to those determined by an indirect fluorescent antibody (IFA) assay for smooth muscle antibody (SMA) IgG, and to determine the prevalence of FAA in patient sera having serologi...

journal_title：Journal of clinical laboratory analysis

authors： Jaskowski TD,Konnick EQ,Ashwood ER,Litwin CM,Hill HR

更新日期：2007-01-01 00:00:00

abstract：:Alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH), which are most abundant in the liver, are the main enzymes involved in ethanol metabolism in humans. Gender-related differences in total liver ADH and ALDH activity among different animal species have been observed in many studies. We measured total ADH an...

journal_title：Journal of clinical laboratory analysis

authors： Chrostek L,Jelski W,Szmitkowski M,Puchalski Z

更新日期：2003-01-01 00:00:00

abstract：OBJECTIVE:This study aimed at exploring the correlation of microRNA (miR)-497/fibroblast growth factor-23 (FGF-23) axis with major adverse cardiac and cerebral event (MACCE) occurrence in end-stage renal disease (ESRD) patients who underwent continuous ambulatory peritoneal dialysis (CAPD). METHODS:Totally, 360 ESRD p...

journal_title：Journal of clinical laboratory analysis

authors： Liu D,Zhou S,Mao H

更新日期：2020-06-01 00:00:00

abstract：BACKGROUND:To discover how NLRP3 and TNFRSF1A polymorphisms affect the efficacy of traditional medicine and etanercept for ankylosing spondylitis (AS) patients. METHODS:Single nucleotide polymorphism (SNP) and haplotype analyses were conducted based on determined NLRP3 and TNFRSF1A among AS patients. We subsequently a...

journal_title：Journal of clinical laboratory analysis

authors： Zhao S,Chen H,Wu G,Zhao C

更新日期：2017-11-01 00:00:00

abstract：INTRODUCTION:Despite advances in diagnostic techniques, many cases of acute myeloid leukemia (AML) remain underdiagnosed in remote centers unequipped with these technologies. We hypothesized that the automated cellular indices with scatter plots and flags may aid in rapid and cost-effective screening of AML. METHODS:C...

journal_title：Journal of clinical laboratory analysis

authors： Virk H,Varma N,Naseem S,Bihana I,Sukhachev D

更新日期：2019-02-01 00:00:00

abstract：BACKGROUND:Reference intervals are important for interpretation of clinical laboratory tests. The platelet (PLT) indices such as the mean platelet volume (MPV) and platelet distribution width (PDW) are newer hematological parameters, which have been recently reported as clinically valuable biomarkers. However, there ar...

journal_title：Journal of clinical laboratory analysis

authors： Hong J,Min Z,Bai-shen P,Jie Z,Ming-ting P,Xian-zhang H,Xiao-ke H,Lan-lan W,Xin Z,Wei G,Rui Q,Wen-xiang C,Xin-zhong W,Yue-yun M,Hong S

更新日期：2015-01-01 00:00:00

abstract：BACKGROUND:Cell-free DNA (cfDNA) in the plasma of patients with both malignant and benign breast lesions was analyzed to determine whether the findings may have diagnostic and prognostic implications and to analyze the association between the levels of cfDNA and prognostic parameters. METHODS:Plasma samples were obtai...

journal_title：Journal of clinical laboratory analysis

authors： Hashad D,Sorour A,Ghazal A,Talaat I

更新日期：2012-11-01 00:00:00

abstract：:A flow cytometric method for quantitation of glutathione (GSH) was applied to simultaneous analysis of the major leukocyte types in peripheral blood. Cellular thiols (predominantly GSH) were stained with monochlorobimane (MCIB), and thiol fluorescence was measured with a flow cytometer. The fluorescence of the thiols ...

journal_title：Journal of clinical laboratory analysis

authors： Scott RB,Collins JM,Matin S,White F,Swerdlow PS

更新日期：1990-01-01 00:00:00

abstract：OBJECTIVE:To explore the clinical value of serum IgM and IgG to SARS-CoV-2 in COVID-19. METHODS:105 COVID-19 patients were enrolled as the disease group. 197 non-COVID-19 patients served as the control group. Magnetic chemiluminescent immunoassay (MCLIA) was used to detect the IgM and IgG. RESULTS:The peak of positiv...

journal_title：Journal of clinical laboratory analysis

authors： Wu LX,Wang H,Gou D,Fu G,Wang J,Guo BQ

更新日期：2020-11-13 00:00:00

abstract：BACKGROUND:Technical innovation of autoimmune blistering dermatoses (ABDs) diagnosis aimed at multiplex approach. Two multiparametric ELISA tests are commercially available for ABDs serology. The aim was to compare diagnostic accuracy of multiparametric and monospecific ELISAs and to examine the diagnostic value/agreem...

journal_title：Journal of clinical laboratory analysis

authors： Gornowicz-Porowska J,Seraszek-Jaros A,Bowszyc-Dmochowska M,Bartkiewicz P,Kaczmarek E,Dmochowski M

更新日期：2018-05-01 00:00:00

abstract：:Congenital hypoplastic anemias are a rare and heterogeneous group of disorders. This paper reviews new molecular diagnostic tests in two distinct forms of congenital anemias, anemia due to transplacental infection with B19 parvovirus and Fanconi anemia. In both instances, molecular assays making use of amplification o...

journal_title：Journal of clinical laboratory analysis

authors： Auerbach AD,Verlander PC,Brown KE,Liu JM

更新日期：1997-01-01 00:00:00

abstract：BACKGROUND:Non-acrocentric satellited chromosomes mostly result from familial balanced insertions or translocations with p12 or p13 of any acrocentric. Although all non-acrocentrics have been involved, only 12 instances of chromosome 6 involvement are known. CASE PRESENTATION:A female infant exhibited clinical feature...

journal_title：Journal of clinical laboratory analysis

authors： Domínguez MG,Rivera H,Dávalos-Pulido RM,Dávalos-Rodríguez IP

更新日期：2020-08-01 00:00:00

abstract：BACKGROUND:Screening tests are available to determine immunity to vaccine-preventable diseases, such as mumps and rubella. We aimed to define better assay for detecting immune status of health care personnel to vaccine-preventable diseases. METHODS:Mumps and rubella antibodies of health care personnel at Shimane Unive...

journal_title：Journal of clinical laboratory analysis

authors： Kumakura S,Shibata H,Isobe T,Hirose M,Ohe M,Nishimura N,Onoda K,Nagai A,Yamaguchi S

更新日期：2013-09-01 00:00:00

abstract：OBJECTIVE:To explore the role of serum periostin in patients with aneurysmal subarachnoid hemorrhage (aSAH). METHOD:We conducted a retrospective study and 124 aSAH patients treated in Shenzhen People's hospital during March 1st 2015 to December 30th 2016 were included. Baseline information, neurological status and cli...

journal_title：Journal of clinical laboratory analysis

authors： Luo W,Wang H,Hu J

更新日期：2018-06-01 00:00:00

abstract：:The two serum proteins of the complement cascade in the highest concentrations, C3 and C4, respond to various conditions in much the same manner as do other positive acute-phase proteins. A major difference is that they are relatively sluggish in response to cytokine drive, requiring several days rather than hours to ...

journal_title：Journal of clinical laboratory analysis

authors： Ritchie RF,Palomaki GE,Neveux LM,Navolotskaia O,Ledue TB,Craig WY

更新日期：2004-01-01 00:00:00

abstract：:The Brazilian External Quality Assessment Program in Immunohematology (BEQAPI) was introduced with the objective of evaluating the quality of diagnosis in immunohematology. From 1992 to 2003, proficiency tests for ABO grouping, Rh (D, C, c, E, e), K phenotyping, direct antiglobulin testing (DAT), antibody screening (A...

journal_title：Journal of clinical laboratory analysis

authors： Melo L,Pellegrino J Jr,Bianco C,Castilho L

更新日期：2005-01-01 00:00:00

abstract：BACKGROUND:The human epididymis protein 4 (HE4) may have high specificity in the detection of malignant diseases, making the development of an immunoassay for HE4 essential. METHODS:In our study, a fusion gene was constructed encoded with the HE4 protein. This protein was then produced in the bacterial cells (Escheric...

journal_title：Journal of clinical laboratory analysis

authors： Zhou L,Lv Z,Shao J,Xu Y,Luo X,Zhang Y,Hu Y,Zhang W,Luo S,Fang J,Wang Y,Duan C,Huang R

更新日期：2016-09-01 00:00:00

abstract：:The present study was designed to evaluate whether the use of acid citrate dextrose (ACD) Formula A may enhance the survival of platelets during storage, thus allowing the continuance of platelet studies over the period of 2-3 hours usually recommended. For this purpose the effects of time on in vitro platelet respons...

journal_title：Journal of clinical laboratory analysis

authors： Pignatelli P,Pulcinelli FM,Ciatti F,Pesciotti M,Ferroni P,Gazzaniga PP

更新日期：1996-01-01 00:00:00

abstract：BACKGROUND:Acute coronary syndrome (ACS) is the leading cause of death in developing and developed countries, yet assessing the risk of its development remains challenging. Several lines of evidence indicate that small, dense low-density lipoproteins (sd-LDL) are associated with increased cardiovascular disease risk. W...

journal_title：Journal of clinical laboratory analysis

authors： Wu B,Yu Z,Tong T,Tong X,Yang Y,Tang Y,Ren H,Liao Y,Liao J

更新日期：2020-03-01 00:00:00

abstract：:Hypoxia and ischemia play important roles in the onset and progression of glaucoma. Insulin-like growth factors (IGF) are important neurotrophic agents that respond to hypoxia-ischemia. In this study, we enrolled 60 primary open angle glaucoma (POAG) patients and 104 healthy volunteers from the China Medical College H...

journal_title：Journal of clinical laboratory analysis

authors： Tsai FJ,Lin HJ,Chen WC,Chen HY,Fan SS

更新日期：2003-01-01 00:00:00

abstract：:One of the most significant achievements of the biochemist during the past two decades is the use to which immunologically based assays have been put in clinical diagnosis (Hood et al.: Immunology, 1984). The problem faced and surmounted by immunologists in effecting the transition from research tool to routine clinic...

journal_title：Journal of clinical laboratory analysis

authors： Nicholls PJ,Malcolm AD

更新日期：1989-01-01 00:00:00

abstract：BACKGROUND:Lupus nephritis is one of the most serious manifestations of systemic lupus erythematosus (SLE). Novel biomarkers are necessary to enhance the diagnostic accuracy, prognostic stratification, monitoring of treatment response, and detection of early renal flares. METHODS:Our study was conducted on 90 particip...

journal_title：Journal of clinical laboratory analysis

authors： Mohammed MF,Belal D,Bakry S,Marie MA,Rashed L,Eldin RE,El-Hamid SA

更新日期：2014-07-01 00:00:00

abstract：:Clinicians recognize and compensate for limitations in estimating the glomerular filtration rate (GFR) using serum creatinine (sCr) measurements by the use of timed collections and mathematical manipulations of sCr. These limitations stem from that fact that sCr is affected by nonrenal influences, including muscle mas...

journal_title：Journal of clinical laboratory analysis

authors： Massey D

更新日期：2004-01-01 00:00:00

abstract：BACKGROUND:The aim of this study is to evaluate the clinical significance of cystatin C(CysC) in the newborns who show normal serum creatinine (Cr) and who are in an intensive care unit. METHODS:From July 2009 to May 2010, a total of 106 patients (53 male and 53 female newborns) in a neonatal intensive care unit at Ky...

journal_title：Journal of clinical laboratory analysis

authors： Cho SY,Hahn WH,Lee HJ,Suh JT,Lee A,Cho BS,Suh JS

更新日期：2012-07-01 00:00:00

abstract：:It is believed that boundary compositions of matrix proteins might play a role in stone formation; however, few proteomic studies concerning matrix proteins in urinary stones have been conducted. In this study, we extracted low molecular weight proteins from calcium oxalate stones and measured their characteristic pat...

journal_title：Journal of clinical laboratory analysis

authors： Chen WC,Lai CC,Tsai Y,Lin WY,Tsai FJ

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:Modern automated laboratory hematology analyzers allow the measurement of over 30 different hematological parameters useful in the diagnostic and clinical interpretation of patient symptoms. They use different methods to measure the same parameters. Thus, a comparison of complete blood count made by Mindray ...

journal_title：Journal of clinical laboratory analysis

authors： Ciepiela O,Kotuła I,Kierat S,Sieczkowska S,Podsiadłowska A,Jenczelewska A,Księżarczyk K,Demkow U

更新日期：2016-11-01 00:00:00

abstract：:The technique that allows for in situ detection of PCR-amplified DNA and cDNA has improved remarkably quickly over the past 5 years. As of this writing, one can expect good results after a relatively quick "learning curve." The technique demands some skill in working with glass slides and cells/tissues. Thus, I recomm...

journal_title：Journal of clinical laboratory analysis

authors： Nuovo GJ

更新日期：1996-01-01 00:00:00

abstract：:Recombinant HIV-1 p66 (rp66, a subunit of reverse transcriptase (RT), a heterodimer of p66 and p51) was produced in Escherichia coli in three different ways. First, rp66 was produced as a part of the fusion protein of lacZ protein and HIV-1 pol protein consisting of three components: protease (p10), RT (p51/p66), and ...

journal_title：Journal of clinical laboratory analysis

authors： Hashinaka K,Nishikata I,Hashida S,Adachi A,Oka S,Ishikawa E

更新日期：2000-01-01 00:00:00

abstract：BACKGROUND:It has been identified that incidence of infertility was about 20% among couples worldwide, about 50% caused by male elements. However, conventional semen laboratory detections could not handle clinical needs, which led to more comprehensive parameters for male fertility evaluation. We aimed to investigate t...

journal_title：Journal of clinical laboratory analysis

authors： Lu R,Chen X,Yu W,Jiang F,Zhou X,Xu Y,Wang F

更新日期：2020-09-14 00:00:00