Identical IgM antibodies recognizing a glycine-alanine epitope are induced during acute infection with Epstein-Barr virus and cytomegalovirus.

abstract：BACKGROUND:Chronic hepatitis B (CHB) is one of the major infectious diseases in which CD4+ T helper (Th) cells play a crucial role. Th9 cells are a distinct subset of CD4+ Th cells with important physiological functions. OBJECTIVE:The study aimed to assess the potential involvement of Th9 cells in the inadequate immun...

journal_title：Journal of clinical laboratory analysis

authors： Cui M,Lv Y,Lu J,Zhang W,Duan Y,Huang Y,Yang L,Li M,Liu W,Liu D,Yan H

更新日期：2018-02-01 00:00:00

abstract：:We studied 103 patients seen in our Prostate Cancer Detection Clinic to determine whether a correlation exists between serum prostate-specific antigen (PSA) values and ultrasound-calculated prostate gland volume. Seventy men (68%) had a PSA value less than or equal to 4 ng/ml (our upper limit of normal). The men were ...

journal_title：Journal of clinical laboratory analysis

authors： Babaian RJ,Fritsche HA,Evans RB

更新日期：1990-01-01 00:00:00

abstract：BACKGROUND:Risperidone (RIS) is a widely used atypical antipsychotic drug. We developed and validated a sensitive and accurate LC-MS/MS method, which requires a small-volume of plasma and small-volume injection for measurement of RIS levels in ASD pediatric patients. We also investigated the relationship between RIS le...

journal_title：Journal of clinical laboratory analysis

authors： Vanwong N,Prommas S,Puangpetch A,Hongkaew Y,Nuntamool N,Nakorn CN,Ngamsamut N,Limsila P,Sukasem C

更新日期：2016-11-01 00:00:00

abstract：BACKGROUND:MicroRNAs play a vital role in coronary artery disease. Abnormal expression of microRNAs has been found to be associated with the occurrence of CAD. METHODS:We identified significantly differentially expressed microRNAs in plasma between 40 patients with CAD and 10 controls with NCA using RNA sequencing. Th...

journal_title：Journal of clinical laboratory analysis

authors： Zhong Z,Zhong W,Zhang Q,Zhang Q,Yu Z,Wu H

更新日期：2020-01-01 00:00:00

abstract：INTRODUCTION:The Mindray CAL 8000 is a cellular analysis line that consists of the BC-6800, an automated hematology analyzer, and the SC-120, an automated slidemaker/stainer. We evaluated the performances of the BC-6800 and the SC-120. METHODS:Four hundred and eight normal and abnormal samples were analyzed. The perfo...

journal_title：Journal of clinical laboratory analysis

authors： Lee HT,Park PW,Seo YH,Kim KH,Seo JY,Jeong JH,Kim MJ,Ahn JY

更新日期：2017-07-01 00:00:00

abstract：:Enteroviruses (EVs) are the most commonly identified cause of aseptic meningitis. Rapid detection and characterization of EV meningitis is essential in making decisions for patient management and treatment. A total of 52 cases of acute aseptic meningitis that occurred from March 2003 to April 2005 were investigated fo...

journal_title：Journal of clinical laboratory analysis

authors： Dumaidi K,Frantzidou F,Papa A,Diza E,Antoniadis A

更新日期：2006-01-01 00:00:00

abstract：BACKGROUND:Modern automated laboratory hematology analyzers allow the measurement of over 30 different hematological parameters useful in the diagnostic and clinical interpretation of patient symptoms. They use different methods to measure the same parameters. Thus, a comparison of complete blood count made by Mindray ...

journal_title：Journal of clinical laboratory analysis

authors： Ciepiela O,Kotuła I,Kierat S,Sieczkowska S,Podsiadłowska A,Jenczelewska A,Księżarczyk K,Demkow U

更新日期：2016-11-01 00:00:00

abstract：BACKGROUND:The study evaluated the impact of interferences on the analytical specificity of three commercial and commonly used creatinine methods (two Jaffe and one enzymatic). METHODS:Manufacturer creatinine methods plus modified methods were tested with the following interferences: spiking serum with bilirubin, albu...

journal_title：Journal of clinical laboratory analysis

authors： Srisawasdi P,Chaichanajarernkul U,Teerakanjana N,Vanavanan S,Kroll MH

更新日期：2010-01-01 00:00:00

abstract：:α-Feto protein (AFP) is the widely used tumor marker in the diagnosis of hepatocellular carcinoma (HCC). The aim of this study was to assess the diagnostic and prognostic validity of a novel marker, serum Glypican-3 (GPC3) and to compare AFP in patients with HCC. One hundred and twenty-eight patients (75 patients with...

journal_title：Journal of clinical laboratory analysis

authors： Ozkan H,Erdal H,Koçak E,Tutkak H,Karaeren Z,Yakut M,Köklü S

更新日期：2011-01-01 00:00:00

abstract：PURPOSE:The aim of this study was to investigate the value of biochemical profile of cyst fluid and diffusion-weighted imaging (DWI) in differentiating hepatic hydatid cysts (HCs) from liver simple cysts. MATERIALS AND METHODS:Forty-six patients underwent MR imaging. Twenty-nine patients had 29 hydatid cysts and 17 pa...

journal_title：Journal of clinical laboratory analysis

authors： Shanshan W,Hui L,Yan L,Li W,Yongfang R,Yan W,Kader M,Wenxiao J

更新日期：2018-01-01 00:00:00

abstract：:Recombinant HIV-1 p66 (rp66, a subunit of reverse transcriptase (RT), a heterodimer of p66 and p51) was produced in Escherichia coli in three different ways. First, rp66 was produced as a part of the fusion protein of lacZ protein and HIV-1 pol protein consisting of three components: protease (p10), RT (p51/p66), and ...

journal_title：Journal of clinical laboratory analysis

authors： Hashinaka K,Nishikata I,Hashida S,Adachi A,Oka S,Ishikawa E

更新日期：2000-01-01 00:00:00

abstract：BACKGROUND:This study aimed to determine whether CD44 polymorphisms were correlated with hepatocellular carcinoma (HCC) and to reveal a new potential target for early prediction, prevention, and diagnosis of HCC. METHOD:This study involved 96 cases with chronic hepatitis B (CHB), 96 cases with hepatitis B virus-relate...

journal_title：Journal of clinical laboratory analysis

authors： Deng Y,Chen ZJ,Lan F,He QT,Chen SY,Du YF,Li S,Qin X

更新日期：2019-10-01 00:00:00

abstract：:The shortcomings of current methods of basophil enumeration detract from the clinical value of the basophil count. Moreover, sophisticated and costly techniques of automated basophil counting hardly can be validated for lack of a suitable reference method. We investigated whether a flow cytometric technique using doub...

journal_title：Journal of clinical laboratory analysis

authors： Hübl W,Andert S,Erath A,Streicher J,Bayer PM

更新日期：1996-01-01 00:00:00

abstract：:We studied the reliability of new indirect tests in the diagnosis of acute infection by Epstein-Barr virus (EBV). Studied for all samples were method 1, the heterophil antibodies (Abs) (Monolatex, Biokit, Germany); method 2, the IgM Abs to EBV with ELISA tests (antigen pools, Enzygnost, Behring-werke, Germany); method...

journal_title：Journal of clinical laboratory analysis

authors： Gutiérrez J,Rodríguez M,Maroto C,Piédrola G

更新日期：1997-01-01 00:00:00

abstract：:The relationship between the levels of serum cystatin C and the prognostic stages of IgA nephropathy was determined in a multicenter trial in Japan. The levels of serum cystatin C in patients with IgA nephropathy were measured using the Dade Behring N Latex Cystain C assay. In 1995, the Joint Committee of the Special ...

journal_title：Journal of clinical laboratory analysis

authors： Tomino Y,Suzuki S,Gohda T,Kobayashi M,Horikoshi S,Imai H,Saito T,Kawamura T,Yorioka N,Harada T,Yasumoto Y,Kida H,Kobayashi Y,Endoh M,Sato H,Saito K

更新日期：2001-01-01 00:00:00

abstract：BACKGROUND:Cell-free DNA (cfDNA) circulating in blood is currently used for noninvasive diagnostic and prognostic tests. Minimizing background DNA is vital for detection of low abundance cfDNA. We investigated whether a new blood collection device could reduce background levels of genomic DNA (gDNA) in plasma compared ...

journal_title：Journal of clinical laboratory analysis

authors： Norton SE,Luna KK,Lechner JM,Qin J,Fernando MR

更新日期：2013-07-01 00:00:00

abstract：:Ninety semen samples from male patients were studied. They were classified into 4 different groups; normospermic fertile, oligospermia, asthenospermia, and oligoasthenospermia. In addition to routine semen analysis, the hypo-osmotic swelling test was done for all these samples, measuring total protein and specific gra...

journal_title：Journal of clinical laboratory analysis

authors： al-Mogazy HM,al-Khadra S,Kenawy G,al-Zeftawy A,Mostafa T,Attia A,Attia AM

更新日期：1993-01-01 00:00:00

abstract：:We described an automated enzymatic assay for conjugated bilirubin (Bc) in serum using the Iatro D-Bil kit, with bilirubin oxidase (EC 1.3.3.5 BOD) from Myrothecium species. The specificity of the enzyme in the Iatro D-Bil kit was examined by analyzing unconjugated bilirubin (Bu), delta bilirubin (Bdelta), and Bc with...

journal_title：Journal of clinical laboratory analysis

authors： Kimura S,Iyama S,Yamaguchi Y,Hayashi S,Yanagihara T

更新日期：1999-01-01 00:00:00

abstract：OBJECTIVE:This study aimed at exploring the correlation of microRNA (miR)-497/fibroblast growth factor-23 (FGF-23) axis with major adverse cardiac and cerebral event (MACCE) occurrence in end-stage renal disease (ESRD) patients who underwent continuous ambulatory peritoneal dialysis (CAPD). METHODS:Totally, 360 ESRD p...

journal_title：Journal of clinical laboratory analysis

authors： Liu D,Zhou S,Mao H

更新日期：2020-06-01 00:00:00

abstract：:Urinary proteins from six patients with esophageal cancer and two with stomach cancer were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Analyses were performed on days-1 to 3, 5, 7, 10, 14, and 21 (or 22) after surgery. The protein patterns were scanned by densitometry and divided ...

journal_title：Journal of clinical laboratory analysis

authors： Yokomizo K,Iijima S,Sakai N,Kurihara Y,Hitratsuka N,Nagai K,Iwai T,Shiba K

更新日期：2005-01-01 00:00:00

abstract：BACKGROUND:This study aimed to investigate the status of internal quality control (IQC) for cardiac biomarkers from 2011 to 2016 so that we can have overall knowledge of the precision level of measurements in China and set appropriate precision specifications. METHODS:Internal quality control data of cardiac biomarker...

journal_title：Journal of clinical laboratory analysis

authors： Li T,Wang W,Zhao H,He F,Zhong K,Yuan S,Wang Z

更新日期：2018-05-01 00:00:00

abstract：OBJECTIVES:In this paper, a novel, sensitive and rapid method to simultaneously determine the free and total prostate-specific antigen (fPSA and tPSA) in serum by combining a time-resolved fluoroimmunoassay (TRFIA) and immunomagnetic separation was described. METHODS:The new approach uses magnetic particles as an immo...

journal_title：Journal of clinical laboratory analysis

authors： Zhou B,Zhang J,Lv Z,Fan J,Zhang Y,Huang B

更新日期：2017-11-01 00:00:00

abstract：BACKGROUND:The association between genetic variations of vascular endothelial growth factor (VEGF) gene and the risk for atherosclerosis has been hypothesized. We aimed to assess the relationship between rs2010963 (+405 C/G) polymorphism and presence, severity, and outcome of coronary artery disease (CAD) in an Iranian...

journal_title：Journal of clinical laboratory analysis

authors： Kalayi Nia S,Ziaee S,Boroumand MA,Sotudeh Anvari M,Pourgholi L,Jalali A

更新日期：2017-07-01 00:00:00

abstract：BACKGROUND:The use of dried blood spots (DBS) for the assay of lysosomal enzymes has facilitated the implementation of pilot studies for newborn screening for lysosomal storage disorders in various developed countries. The aim of the study was to determine the influence of ambient temperature during DBS preparation and...

journal_title：Journal of clinical laboratory analysis

authors： Supriya M,De T,Christopher R

更新日期：2018-01-01 00:00:00

abstract：:Congenital heart disease (CHD) is a common cardiac defect found in infants and children. Despite advances in diagnosis and treatment, our understanding of the causative mechanism and etiology of CHD is limited. To determine the genetic etiology of CHD, we selected 11 consecutive short tandem-repeat polymorphic (STRP) ...

journal_title：Journal of clinical laboratory analysis

authors： Shi YR,Hsieh KS,Wu JY,Lee CC,Tsai CH,Yu MT,Chang JS,Tsai FJ

更新日期：2003-01-01 00:00:00

abstract：AIM:To evaluate, retrospectively, the frequency of autoantibodies of antiphospholipid syndrome (APLS) in Tunisian patients with primary biliary cirrhosis (PBC). PATIENTS AND METHODS:We analyzed 80 PBC sera and 80 sera from blood donors. ELISA was used to determine the frequency of antibodies against cardiolipin (aCL I...

journal_title：Journal of clinical laboratory analysis

authors： Mankaï A,Manoubi W,Ghozzi M,Melayah S,Sakly W,Ghedira I

更新日期：2015-01-01 00:00:00

abstract：BACKGROUND:Oxidative stress is an imbalance between the reactive oxygen species and antioxidant system. In this study, total oxidative stress (TOS) and total antioxidant capacity (TAC) were investigated with a new and practical method in childhood iron-deficiency anemia. METHOD:During the study period 80 children betw...

journal_title：Journal of clinical laboratory analysis

authors： Akça H,Polat A,Koca C

更新日期：2013-05-01 00:00:00

abstract：:A flow cytometric method for quantitation of glutathione (GSH) was applied to simultaneous analysis of the major leukocyte types in peripheral blood. Cellular thiols (predominantly GSH) were stained with monochlorobimane (MCIB), and thiol fluorescence was measured with a flow cytometer. The fluorescence of the thiols ...

journal_title：Journal of clinical laboratory analysis

authors： Scott RB,Collins JM,Matin S,White F,Swerdlow PS

更新日期：1990-01-01 00:00:00

abstract：:Staphylococcus lugdunensis is an unusually virulent coagulase-negative species, associated with severe infections. The present report describes the development of a single-step, species-specific PCR protocol for S. lugdunensis identification. fbl gene, encoding a fibrinogen-binding adhesin, was exploited and assessed ...

journal_title：Journal of clinical laboratory analysis

authors： Chatzigeorgiou KS,Siafakas N,Petinaki E,Zerva L

更新日期：2010-01-01 00:00:00

abstract：:The pathogenic role of immune-mediated mechanisms in chronic hepatitis C virus (HCV) infection has not yet been elucidated. In this study, we report different cytokine expression profiles from hemodialysis (HD) and non-HD HCV (+) patients. IL-1beta, IL-2, IL-4, IL-6, TNF-alpha, and TGF-beta1 serum levels, and liver bi...

journal_title：Journal of clinical laboratory analysis

authors： Spanakis NE,Garinis GA,Alexopoulos EC,Patrinos GP,Menounos PG,Sklavounou A,Manolis EN,Gorgoulis VG,Valis D

更新日期：2002-01-01 00:00:00