Abstract：
:We studied the reliability of new indirect tests in the diagnosis of acute infection by Epstein-Barr virus (EBV). Studied for all samples were method 1, the heterophil antibodies (Abs) (Monolatex, Biokit, Germany); method 2, the IgM Abs to EBV with ELISA tests (antigen pools, Enzygnost, Behring-werke, Germany); method 3, EA (Biotest Diagnostics, Germany); and method 4, the IgG avidity test. The reliability of the four tests for the detection of primary infection by EBV was: sensitivity (method 1: 89.1%; method 2: 100%; method 3: 79.7%; method 4: 99%); specificity (method 1: 98%; method 2: 100%; method 3: 84%; method 4: 100%); positive predictive value (method 1: 97.6%; method 2: 100%; method 3: 73.6%; method 4: 100%), and negative predictive value (method 1: 90.7%; method 2: 100%; method 3: 84%; method 4: 99%). The IgG avidity test (method 4) is simple and automated in the laboratory and is very useful for ascertaining, from a single sample, the time since infection. It is criteria of recent primoinfection higher levels than 55% of IgG with low avidity for the antigen. The investigation of the Abs to antigen pools (method 2) by ELISA of virus had a high reliability, but the investigation of heterophil Abs by latex (method 1) and the Abs IgM to EA (method 2) were lacking in sensibility regarding their use in the diagnosis of the primoinfection.
journal_name
J Clin Lab Analjournal_title
Journal of clinical laboratory analysisauthors
Gutiérrez J,Rodríguez M,Maroto C,Piédrola Gsubject
Has Abstractpub_date
1997-01-01 00:00:00pages
78-81issue
2eissn
0887-8013issn
1098-2825pii
10.1002/(SICI)1098-2825(1997)11:2<78::AID-JCLA2>3.journal_volume
11pub_type
杂志文章abstract：BACKGROUND:Selection and verification of blood collection tubes is an important preanalytical issue in clinical laboratories. Today, gel tubes are commonly used with many advantages, although they are known to cause interference in immunoassay methods. In this study, we aimed to compare SSTs of two different suppliers ...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.22427
更新日期：2018-07-01 00:00:00
abstract：:In order to establish a rapid staining method sensitive enough to stain the protein bands on cellulose acetate membrane and suitable for the analysis of urinary proteins in healthy subjects, we conducted a detailed examination of Kovarik's method designed for the analysis of proteins in cerebrospinal fluid in detail. ...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2825(1996)10:6<403::AID-JCLA15>
更新日期：1996-01-01 00:00:00
abstract：BACKGROUND:Toxocariasis is the clinical term that is applied to infection in the human host with Toxocara species larvae. Serological tests are important tools for the diagnosis of toxocariasis. The aim of this study was to evaluate the excretory-secretory (ES) antigens of T. cati larvae using enzyme-linked immunosorbe...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.21844
更新日期：2016-05-01 00:00:00
abstract：:A modification of the assay system for peroxidase activity in human mixed saliva using 2,2'-azinobis-(-ethylbenzthiazoline-6-sulphonic acid) (ABTS) as the substrate is reported. The modifications permit differentiation between glandular peroxidase (SPX) and myeloperoxidase (MPX) activity. In addition, factors endogeno...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.1860050111
更新日期：1991-01-01 00:00:00
abstract：:Amyloid peptide is thought to play a critical role in neuronal death in Alzheimer's disease (AD), most likely through oxidative stress. Free radical-related injury leads to DNA breaks, which subsequently activates the repair enzyme poly(ADP-ribose) polymerase-1 (PARP-1). In this study, the relationship between genetic...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.20379
更新日期：2010-01-01 00:00:00
abstract：:The proteoglycan decorin inhibits TGF-beta; therefore, it could antagonize progression of fibrotic diseases associated with activation of TGF-beta(1). The effect of decorin transfection in human mesangial cells (HMCs) on the expression of genes related to kidney fibrosis was investigated. HMCs, isolated from glomeruli...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.10038
更新日期：2002-01-01 00:00:00
abstract：BACKGROUND:It is generally believed that the lower limit of postprandial plasma glucose is the same or higher than that of fasting plasma glucose (FPG). This study aimed to investigate the relationship between 2-h postprandial plasma glucose (2-hPG) and FPG. Insulin sensitivity and β-cell function were also evaluated. ...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.23404
更新日期：2020-09-01 00:00:00
abstract：:Using a fully automated reticulocyte counter, the roles of the reticulocyte count with maturation in pancytopenia were evaluated. Different groups of pancytopenia including aplastic anemia, infiltrative marrow disorder, hypersplenism, and megaloblastic anemia were recruited. All patients had bone marrow examinations f...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.1860070612
更新日期：1993-01-01 00:00:00
abstract：BACKGROUND:Neurofilaments are the major cytoskeletal components of neurons, and cell injury leads to their release into the surrounding area. The aim of this study was to compare the cerebrospinal fluid (CSF) and serum (S) concentrations of neurofilament light chains (NFLs) and phosphorylated neurofilament heavy chains...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.22948
更新日期：2019-09-01 00:00:00
abstract：:Hemoglobin F (HbF) is an effective inhibitor of HbS polymerization. Hydroxyurea (HU) is used to increase HbF synthesis and improve the clinical course of sickle cell disease (SCD) patients. We studied a series of laboratory parameters concerning HbF production and reticulocyte response, and compared data between two g...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.10070
更新日期：2003-01-01 00:00:00
abstract：BACKGROUND:Salmonella is an important foodborne pathogen that causes acute diarrhea in humans worldwide. This study analyzed the relationships of serotypes and antibiotic resistance with virulence genes of Salmonella isolated from children with salmonellosis. METHODS:Serological typing was performed using the slide-ag...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.23525
更新日期：2020-08-14 00:00:00
abstract：BACKGROUND:Non-acrocentric satellited chromosomes mostly result from familial balanced insertions or translocations with p12 or p13 of any acrocentric. Although all non-acrocentrics have been involved, only 12 instances of chromosome 6 involvement are known. CASE PRESENTATION:A female infant exhibited clinical feature...
journal_title：Journal of clinical laboratory analysis
pub_type：
doi：10.1002/jcla.23355
更新日期：2020-08-01 00:00:00
abstract：PURPOSE:The aim of this study was to investigate the value of biochemical profile of cyst fluid and diffusion-weighted imaging (DWI) in differentiating hepatic hydatid cysts (HCs) from liver simple cysts. MATERIALS AND METHODS:Forty-six patients underwent MR imaging. Twenty-nine patients had 29 hydatid cysts and 17 pa...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.22192
更新日期：2018-01-01 00:00:00
abstract：BACKGROUND:The Architect HIV Ag/Ab Combo has excellent performance for HIV screening; however, the false-positive rate (FPR) was high in low HIV prevalence setting. OBJECTIVES:The purpose of this study was to analyze the influence of sample-to-cutoff (s/co) ratios by Architect HIV Ag/Ab Combo with the results of confi...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.22866
更新日期：2019-06-01 00:00:00
abstract：:We studied antibody production in serial serum samples from patients with acute Epstein-Barr virus (EBV) and cytomegalovirus (CMV) infections. Sera were analyzed both by enzyme-linked immunosorbent assay (ELISA) using a synthetic peptide (P62) derived from the glycine-alanine repeating region of the Epstein-Barr nucle...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.1860040613
更新日期：1990-01-01 00:00:00
abstract：:The levels of several soluble cytokine receptors in body fluids of healthy individuals change with age. Clinical application of the measurement of the serum soluble interleukin-1 receptor type I (sIL-1RI) level depends critically on the samples used as the controls. At present, there is no information regarding the le...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.20315
更新日期：2009-01-01 00:00:00
abstract：:Congenital heart disease (CHD) is a common cardiac defect found in infants and children. Despite advances in diagnosis and treatment, our understanding of the causative mechanism and etiology of CHD is limited. To determine the genetic etiology of CHD, we selected 11 consecutive short tandem-repeat polymorphic (STRP) ...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.10062
更新日期：2003-01-01 00:00:00
abstract：BACKGROUND:Previous studies have demonstrated that dysfunctional regulatory T cells (Tregs) may be associated with Graves' disease (GD). In this study, we evaluated four serum Treg-associated miRNAs (miR-210, miR-182, miR-155, and miR-146a) expressions and assessed the potential of serum miRNAs as biomarkers of GD. ME...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.22266
更新日期：2018-02-01 00:00:00
abstract：BACKGROUND:Accumulated evidences indicate that long non-coding RNAs (lncRNAs) participate in many biological mechanisms. Moreover, it acts as an essential regulator in various human diseases such as gastric cancer (GC). Nevertheless, the comprehensive regulatory roles and clinical significance of most lncRNAs in GC are...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.23210
更新日期：2020-05-01 00:00:00
abstract：BACKGROUND:The current transfusion policy recommended for individuals with serologic weak-D phenotype is based on data derived from European-descent populations. Data referring to the distribution of RH alleles underlying weak-D phenotype among people of mixed origin are yet incomplete, and the applicability of Europea...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.22596
更新日期：2018-11-01 00:00:00
abstract：:Two methods for the extraction of RNA of vesicular stomatitis virus (VSV) Indiana1 and New Jersey and their simultaneous amplification by one-step polymerase chain reaction using reverse transcriptase were evaluated. A guanidine-thiocyanate-based RNA extraction (Qiagen RNeasy Mini Kit, Qiagen, Valencia, CA ) followed ...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.20439
更新日期：2011-01-01 00:00:00
abstract：BACKGROUND:Normal serum alanine aminotransferase (ALT) levels differ with age, gender, and body mass index. Adjusting the upper limits of normal (ULN) for ALT needs further research in different populations. Aim of this work was to monitor the effect of successful chronic hepatitis C (CHC) treatment on the ALT levels i...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.22296
更新日期：2018-03-01 00:00:00
abstract：:The newly developed immunoblot assay, RIBA SIA (recombinant and synthetic polypeptide immunoblot assay), Chiron, Calif., was compared with the commercially available second generation recombinant immunoblot assay (RIBA-2) for the detection of antibody to hepatitis C virus (anti-HCV). The two immunoblot tests were also...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.1860070306
更新日期：1993-01-01 00:00:00
abstract：:We aimed to evaluate whether a PEG-IAT method, modified for gel technique, could replace the two-stage enzyme method for routine identification of red blood cell (RBC) antibodies.A total of 161 selected patient samples were analyzed with the two-stage enzyme tube technique and the PEG-IAT in gel techniques. In 65 samp...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.20017
更新日期：2004-01-01 00:00:00
abstract：BACKGROUND:Alport syndrome (ATS) is a hereditary nephritis with hereditary and clinical heterogeneity; the early clinical symptoms are atypical, which can easily lead to misdiagnosis. The proband, a 6-year-old girl, was found to have microscopic hematuria, proteinuria, and visual impairment at about 5 years old; the re...
journal_title：Journal of clinical laboratory analysis
pub_type：
doi：10.1002/jcla.23558
更新日期：2020-09-06 00:00:00
abstract：:It is believed that boundary compositions of matrix proteins might play a role in stone formation; however, few proteomic studies concerning matrix proteins in urinary stones have been conducted. In this study, we extracted low molecular weight proteins from calcium oxalate stones and measured their characteristic pat...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.20214
更新日期：2008-01-01 00:00:00
abstract：:A sensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) for (anti-human T-cell leukemia virus type I) IgG (anti-HTLV-I IgG) in serum using recombinant gag p24(14-214) of HTLV-I is described. The recombinant gag p24(14-214) is soluble in the absence of detergents and allows the use of enzymes other ...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.1860060509
更新日期：1992-01-01 00:00:00
abstract：BACKGROUND:Automated flow cytometry-based urine analyzer is increasingly being used to identify and enumerate cells and particles in urine specimens. It measures electrical conductivity which could be transformed to osmolality. Using this machine, all urine specimens could be screened for osmolality without requiring a...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.23586
更新日期：2020-09-24 00:00:00
abstract：:Previously, an ultrasensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) for p24 antigen of HIV-1 was developed. The immune complex comprising 2,4-dinitrophenyl-biotinyl-bovine serum albumin-rabbit anti-p24 Fab' conjugate, p24 antigen, and rabbit anti-p24 Fab' -beta-D-galactosidase conjugate was t...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2825(1996)10:5<302::AID-JCLA11>
更新日期：1996-01-01 00:00:00
abstract：:A novel severe acute respiratory syndrome-associated coronavirus (SARS-CoV) has been discovered. The detection of both antigens and antibodies in SARS-CoV from human specimens with suspected SARS plays an important role in preventing infection. We developed a novel rapid immunochromatographic test (RICT) based on the ...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.20070
更新日期：2005-01-01 00:00:00
