Simultaneous determination of free and total prostate-specific antigen by a magnetic particle-based time-resolved fluoroimmunoassay.

abstract：:Staphylococcus lugdunensis is an unusually virulent coagulase-negative species, associated with severe infections. The present report describes the development of a single-step, species-specific PCR protocol for S. lugdunensis identification. fbl gene, encoding a fibrinogen-binding adhesin, was exploited and assessed ...

journal_title：Journal of clinical laboratory analysis

authors： Chatzigeorgiou KS,Siafakas N,Petinaki E,Zerva L

更新日期：2010-01-01 00:00:00

abstract：BACKGROUND:Bone marrow (BM) study plays an important role as initial investigation specimen of lymphoma as well as staging lymphoma. This study aimed to investigate the utility of BM studies for classification of lymphoma and evaluate features of BM involvement by lymphoma over a period of 11 years. METHODS:A total of...

journal_title：Journal of clinical laboratory analysis

authors： Kwoun WJ,Ahn JY,Park PW,Seo YH,Kim KH,Seo JY,Lee HT,Yoo KH

更新日期：2019-05-01 00:00:00

abstract：BACKGROUND:Oleander interferes with serum digoxin measurements using various immunoassays. The potential interference of oleander and its active ingredient, oleandrin, with a relatively new homogenous sequential chemiluminescent digoxin assay based on luminescent oxygen channeling technology (LOCI digoxin assay, Siemen...

journal_title：Journal of clinical laboratory analysis

authors： Dasgupta A,Welsh KJ,Hwang SA,Johnson M,Actor JK

更新日期：2014-01-01 00:00:00

abstract：BACKGROUND:The primary test for the laboratory confirmation of measles is immunoglobulin M (IgM) serology. It is therefore important to evaluate new commercial measles IgM immunoassays to ensure high-quality measles diagnostic testing. The purpose of this study was to evaluate the diagnostic performance of LIAISON IgM ...

journal_title：Journal of clinical laboratory analysis

authors： Sampedro A,Rodríguez-Granger J,Gómez C,Lara A,Gutierrez J,Otero A

更新日期：2013-11-01 00:00:00

abstract：:The incidence of aneuploidy was determined by flow cytometric analysis in paraffin-embedded tumor samples of 125 patients with non-Hodgkin lymphoma (NHL). There were 48 low-grade (LG) and 77 high-grade (HG) tumors. Aneuploidy was found in 34 (27%) NHL, 15 (31%) LG, and 19 (25%) HG tumors. The analysis of patient survi...

journal_title：Journal of clinical laboratory analysis

authors： Vucković J,Forenpoher G,Marusić M,Uzarević B,Sabioncello A,Stula N,Dubravcić M

更新日期：1995-01-01 00:00:00

abstract：BACKGROUNDS:Shortening of primary cilia in kidney epithelial cells is associated with kidney injury and involved with the induced level of α-tubulin in urine. Therefore, rapid detection and quantification of α-tubulin in the urine samples could be used to the preliminary diagnosis of kidney injury. METHODS:Cellulose-b...

journal_title：Journal of clinical laboratory analysis

authors： Choi ES,Al Faruque H,Kim JH,Cho JH,Park KM,Kim E

更新日期：2020-01-01 00:00:00

abstract：OBJECTIVE:The coronavirus disease 2019 (COVID-19) has rapidly developed into a pandemic. Increased levels of ferritin due to cytokine storm and secondary hemophagocytic lymphohistiocytosis were found in severe COVID-19 patients. Therefore, the aim of this study was to determine the role of ferritin in COVID-19. METHOD...

journal_title：Journal of clinical laboratory analysis

authors： Cheng L,Li H,Li L,Liu C,Yan S,Chen H,Li Y

更新日期：2020-10-01 00:00:00

abstract：BACKGROUND:Central nervous system (CNS) abnormalities are a group of serious birth defects associated with high rates of stillbirths, infant death, or abnormal development, and various disease-causing copy number variations play a much more important role in the etiology of CNS abnormalities. This study intends to pres...

journal_title：Journal of clinical laboratory analysis

authors： Song T,Xu Y,Li Y,Jia L,Zheng J,Dang Y,Wan S,Zheng Y,Zhang J,Yang H

更新日期：2020-10-01 00:00:00

abstract：:Circulating bilirubin is thought to function as a physiological antioxidant. One of the decomposition products of this process is the biopyrrins, which include two regioisomers: biotripyrrin-a (1,14,15,17-tetrahydro-2,7,13-trimethyl-1,14-deoxy-3-vinyl-16H-tripyrrin-8,12-dipropionic acid) and biotripyrrin-b (1,14,15,17...

journal_title：Journal of clinical laboratory analysis

authors： Ihara H,Matsumoto T,Morita Y,Hirano A,Okada M,Hashizume N,Shioji I,Yoshimura H

更新日期：2007-01-01 00:00:00

abstract：BACKGROUND:The pulmonary surfactant especially lipids in amniotic fluid can reflect the development stage of fetal lung maturity (FLM). However, the conventional lecithin/sphingomyelin (L/S) ratio method by thin layer chromatography (TLC) is insufficient and inconvenient for FLM prediction in clinical practice. METHOD...

journal_title：Journal of clinical laboratory analysis

authors： Cao Z,Liu J,Xie X,Zhan S,Song W,Wu S,Sun Z,Dong Y,Tang G,Liu Y,Li L,Shen M,Zhai Y,Zou J,Liu X

更新日期：2020-04-01 00:00:00

abstract：BACKGROUND:Standards play an important role in detection of the BCR-ABL1 fusion gene (FG) transcript. However, the standards widely used in laboratories are mainly based on plasmids or cDNA, which cannot accurately reflect the process of RNA extraction and cDNA synthesis. Therefore, we aimed to develop armored RNA-base...

journal_title：Journal of clinical laboratory analysis

authors： Fu Y,Zhang R,Wu Q,Zhang J,Bao L,Li J

更新日期：2018-11-01 00:00:00

abstract：:A microparticle-enhanced nephelometric immunoassay was developed for lactoferrin quantitation in human milk. It is based on the nephelometric measurement of the light scattered during the competitive immuno-agglutination of a microparticle-lactoferrin conjugate with an antilactoferrin antiserum. This immunoassay is se...

journal_title：Journal of clinical laboratory analysis

authors： Cuillière ML,Montagne P,Molé C,Béné MC,Faure G

更新日期：1997-01-01 00:00:00

abstract：:A novel and ultrasensitive noncompetitive enzyme immunoassay (hetero-two-site complex transfer enzyme immunoassay) for alpha-human atrial natriuretic peptide (alpha-hANP) in plasma is described. alpha-hANP was biotinylated using N-hydroxysuccinimidobiotin and trapped onto an anti-alpha-hANP [6-28] IgG-coated polystyre...

journal_title：Journal of clinical laboratory analysis

authors： Hashida S,Ishikawa E

更新日期：1992-01-01 00:00:00

abstract：:It has been proposed that sample storage may have some influence on the parameters of oxidative stress status (OSS) in biological fluids. We measured four important OSS parameters in plasma of 23 healthy subjects and repeated the measurements in the same samples kept at -70 degrees C after different time intervals. Hy...

journal_title：Journal of clinical laboratory analysis

authors： Firuzi O,Mladenka P,Riccieri V,Spadaro A,Petrucci R,Marrosu G,Saso L

更新日期：2006-01-01 00:00:00

abstract：:By using commercially available ELISA kits, serum IL-6 and TNF-alpha levels in healthy adults, and the levels of various cytokines in patients with primary biliary cirrhosis or chronic viral liver diseases, were investigated. IL-6 levels of healthy subjects were distributed in a wide range, and the distribution patter...

journal_title：Journal of clinical laboratory analysis

authors： Yamashiki M,Kosaka Y,Nishimura A,Watanabe S,Nomoto M,Ichida F

更新日期：1998-01-01 00:00:00

abstract：:Our objectives in this study were to compare results obtained by an enzyme immunoassay (EIA) for F-actin antibody (FAA) immunoglobulin G (IgG) to those determined by an indirect fluorescent antibody (IFA) assay for smooth muscle antibody (SMA) IgG, and to determine the prevalence of FAA in patient sera having serologi...

journal_title：Journal of clinical laboratory analysis

authors： Jaskowski TD,Konnick EQ,Ashwood ER,Litwin CM,Hill HR

更新日期：2007-01-01 00:00:00

abstract：BACKGROUND:Coronary heart disease (CHD) is primarily caused by atherosclerosis of coronary arteries. It is largely an inflammatory disease of the vascular wall. The inflammation is related to DNA methylation. Angiopoietin-like protein 2 (ANGPTL2) has various functions in several chronic inflammatory diseases. Macrophag...

journal_title：Journal of clinical laboratory analysis

authors： Zhou J,Chen L,Yang X,Huang X,Wang Z,Peng P,Lian J

更新日期：2019-03-01 00:00:00

abstract：:Medical treatment for Helicobacter pylori (Hp) infection is now recommended in several types of gastroduodenal disease, and its success is usually monitored by hystology. The end-points of our work were to identify the most suitable serum index of Hp eradication among pepsinogen A (PGA), pepsinogen C (PGC), PGA/PGC ra...

journal_title：Journal of clinical laboratory analysis

authors： Plebani M,Basso D,Scrigner M,Toma A,Di Mario F,Dal Bò N,Samloff IM

更新日期：1996-01-01 00:00:00

abstract：BACKGROUND:This study aimed to investigate the relationship between HbA1c and RBC aggregation in type 2 diabetes mellitus (T2DM) patients by analysis of data from routine clinical tests and from in vitro experiments. METHODS:A total of 2,111 inpatients with type 2 diabetes were selected and among them, 364 patients (G...

journal_title：Journal of clinical laboratory analysis

authors： Li Q,Li L,Li Y

更新日期：2015-09-01 00:00:00

abstract：BACKGROUND:Selection and verification of blood collection tubes is an important preanalytical issue in clinical laboratories. Today, gel tubes are commonly used with many advantages, although they are known to cause interference in immunoassay methods. In this study, we aimed to compare SSTs of two different suppliers ...

journal_title：Journal of clinical laboratory analysis

authors： Ercan M,Fırat Oğuz E,Akbulut ED,Yilmaz M,Turhan T

更新日期：2018-07-01 00:00:00

abstract：:Clinicians recognize and compensate for limitations in estimating the glomerular filtration rate (GFR) using serum creatinine (sCr) measurements by the use of timed collections and mathematical manipulations of sCr. These limitations stem from that fact that sCr is affected by nonrenal influences, including muscle mas...

journal_title：Journal of clinical laboratory analysis

authors： Massey D

更新日期：2004-01-01 00:00:00

abstract：:Type IV collagen is a major component released from the glomerular and tubular basement membranes. To investigate the alteration of renal type IV collagen turnover in early stage diabetic nephropathy, urinary type IV collagen was measured by a highly sensitive one-step sandwich enzyme immunoassay (EIA). Urinary sample...

journal_title：Journal of clinical laboratory analysis

authors： Iijima T,Suzuki S,Sekizuka K,Hishiki T,Yagame M,Jinde K,Saotome N,Suzuki D,Sakai H,Tomino Y

更新日期：1998-01-01 00:00:00

abstract：BACKGROUND:Gastrin-17 (G-17) and Helicobacter pylori (H pylori) antibody are widely used in the screening of gastric diseases, especially in gastric cancer. In this study, we aimed to evaluate the value of G-17 and H pylori antibody in gastric disease screening. METHODS:Healthy males and females (1368 and 1212, respec...

journal_title：Journal of clinical laboratory analysis

authors： Liu W,Sun Y,Yuan Y

更新日期：2020-08-30 00:00:00

abstract：AIMS:Essential hypertension (EH) is a high prevalence disease facing a public health challenge. People were little known about the genetics of diagnosing the cause of EH. Circular RNAs that have a continuous cycle of covalent closure, without affected by RNA exonuclease, and are more stable and hard to degrade may invo...

journal_title：Journal of clinical laboratory analysis

authors： Bao X,He X,Zheng S,Sun J,Luo Y,Tan R,Zhao J,Zhong F,Zhang L

更新日期：2019-05-01 00:00:00

abstract：:A sensitive time-resolved fluorimetric immune-complex-transfer immunoassay for antithyroglobulin IgG in serum is described. Antithyroglobulin IgG in test serum was reacted with dinitrophenyl europium ion-labeled thyroglobulin. The complex formed of antithyroglobulin IgG and dinitrophenyl europium ion-labeled thyroglob...

journal_title：Journal of clinical laboratory analysis

authors： Kohno T,Mitsukawa T,Matsukura S,Ishikawa E

更新日期：1990-01-01 00:00:00

abstract：BACKGROUND:Reference intervals are critical for interpreting test results of a clinical laboratory. The aim of this study was to establish local reference intervals of complete blood count for healthy preschoolers in China. METHODS:Three thousand eight hundred and twenty-one blood specimens from children aged 4 months...

journal_title：Journal of clinical laboratory analysis

authors： Wang GC,Li N,Niu C,Ma WB,Wang ZL,Guo H,Han LH

更新日期：2017-09-01 00:00:00

abstract：:We investigated the relationship between the urokinase type plasminogen activator receptor (uPAR) in sera and tissues of patients with cervical cancer and the clinical and pathological features of the cancer. Immunohistochemistry (SABC method) was used to detect uPAR expression in cervical cancer and normal tissues; E...

journal_title：Journal of clinical laboratory analysis

authors： Jing J,Zheng S,Han C,Du L,Guo Y,Wang P

更新日期：2012-01-01 00:00:00

abstract：:The Venereal Disease Research Laboratory (VDRL) test has long been considered the best serological test for the diagnosis of neurosyphilis. The goal of this study was to find out if the Rapid Plasma Reagin (RPR) could be an alternative to the VDRL. Cerebrospinal fluid (CSF) and sera samples from patients in the follow...

journal_title：Journal of clinical laboratory analysis

authors： Castro R,Prieto ES,da Luz Martins Pereira F

更新日期：2008-01-01 00:00:00

abstract：BACKGROUNDS:β-Thalassemia is one of the most prevalent worldwide autosomal recessive disorders. It presents a great molecular heterogeneity resulting from more than 200 causative mutations in the β-globin gene. In Tunisia, β-thalassemia represents the most prevalent monogenic hemoglobin disorder with 2.21% of carriers....

journal_title：Journal of clinical laboratory analysis

authors： Sahli CA,Ben Salem I,Jouini L,Laouini N,Dabboubi R,Hadj Fredj S,Siala H,Othmeni R,Dakhlaoui B,Fattoum S,Bibi A,Messaoud T

更新日期：2016-09-01 00:00:00

abstract：OBJECTIVE:A common problem in clinical laboratories is maintaining the stability of analytes during pre-analytical processes. The aim of this study was to systematically summarize the results of a set of studies about the biochemical analytes stability. METHODS:A literature search was performed on the Advanced search ...

journal_title：Journal of clinical laboratory analysis

authors： Hedayati M,Razavi SA,Boroomand S,Kheradmand Kia S

更新日期：2020-09-01 00:00:00