Enzymatic assay for conjugated bilirubin (Bc) in serum using bilirubin oxidase (BOD).

abstract：:The elevation of chylomicrons and chylomicron remnants in plasma would lead to hyperlipidemia and other complications. Apo B-48, which is translated and produced in the adult intestine from the same gene as Apo B-100, is considered to be an essential component of chylomicrons and chylomicron remnants. Using a peptide ...

journal_title：Journal of clinical laboratory analysis

authors： Uchida Y,Kurano Y,Ito S

更新日期：1998-01-01 00:00:00

abstract：BACKGROUND:Newborn screening tests have been designed to identify infants with severe disorders that are relatively prevalent and treatable or controllable. Comparing to other countries, the incidence of these diseases are very high in Turkey where the rate of consanguineous marriage is high. METHODS:In this article, ...

journal_title：Journal of clinical laboratory analysis

authors： Tezel B,Dilli D,Bolat H,Sahman H,Ozbaş S,Acıcan D,Ertek M,Köse MR,Dilmen U

更新日期：2014-01-01 00:00:00

abstract：BACKGROUND:This study was performed to investigate the epidemiology of enterovirus (EV) infections in children at a single hospital during recent 5 years. This study was preformed from December 2008 to November 2013. METHODS:We subjected 1,067 cerebrospinal fluid (CSF) specimens from 1,053 patients and 678 stool speci...

journal_title：Journal of clinical laboratory analysis

authors： Jeon JS,Kim JK,Yu J,Kim JW

更新日期：2016-03-01 00:00:00

abstract：:A sensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) for (anti-human T-cell leukemia virus type I) IgG (anti-HTLV-I IgG) in serum using recombinant gag p24(14-214) of HTLV-I is described. The recombinant gag p24(14-214) is soluble in the absence of detergents and allows the use of enzymes other ...

journal_title：Journal of clinical laboratory analysis

authors： Kohno T,Hirota K,Sakoda I,Yamasaki M,Yokoo Y,Ishikawa E

更新日期：1992-01-01 00:00:00

abstract：BACKGROUND:DRI cotinine assay is suitable only for screening for cotinine in urine specimens. We studied the reliability of DRI cotinine semiquantitative values by comparing them with the cotinine concentration obtained with a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method. METHODS:Semiquantitative c...

journal_title：Journal of clinical laboratory analysis

authors： Dixon RB,Dasgupta A

更新日期：2016-11-01 00:00:00

abstract：BACKGROUND:It has been identified that incidence of infertility was about 20% among couples worldwide, about 50% caused by male elements. However, conventional semen laboratory detections could not handle clinical needs, which led to more comprehensive parameters for male fertility evaluation. We aimed to investigate t...

journal_title：Journal of clinical laboratory analysis

authors： Lu R,Chen X,Yu W,Jiang F,Zhou X,Xu Y,Wang F

更新日期：2020-09-14 00:00:00

abstract：:The shortcomings of current methods of basophil enumeration detract from the clinical value of the basophil count. Moreover, sophisticated and costly techniques of automated basophil counting hardly can be validated for lack of a suitable reference method. We investigated whether a flow cytometric technique using doub...

journal_title：Journal of clinical laboratory analysis

authors： Hübl W,Andert S,Erath A,Streicher J,Bayer PM

更新日期：1996-01-01 00:00:00

abstract：OBJECTIVES:Existing evidence suggests that impaired vitamin D metabolism contribute to the development of atherosclerosis. Aortic intima-media thickness (IMT) is an earlier marker than carotid IMT of preclinical atherosclerosis. However, there is a lack of researches on direct investigation of relevance between serum 2...

journal_title：Journal of clinical laboratory analysis

authors： Kalkan GY,Gür M,Koyunsever NY,Şeker T,Gözükara MY,Uçar H,Kaypaklı O,Baykan AO,Akyol S,Türkoğlu C,Elbasan Z,Şahin DY,Çaylı M

更新日期：2015-07-01 00:00:00

abstract：BACKGROUND:Donor-specific anti-human leukocyte antigen (HLA) antibodies (DSAs) in recipients is a risk factor for donor stem cell graft failure in haploidentical hematopoietic stem cell transplantation (haplo-HSCT), and the treatment to reduce the levels of DSAs is not unanimous. This study was to analysis the role of ...

journal_title：Journal of clinical laboratory analysis

authors： Zhang R,He Y,Yang D,Jiang E,Ma Q,Pang A,Zhai W,Wei J,Feng S,Han M

更新日期：2020-07-01 00:00:00

abstract：BACKGROUND:A convenient biosensor for real-time measurement of biomarkers for in-field psychophysiological stress research and military operations is desirable. We evaluated a hand-held device for measuring salivary amylase as a stress marker in medical technicians undergoing combat casualty care training using two dif...

journal_title：Journal of clinical laboratory analysis

authors： Peng HT,Savage E,Vartanian O,Smith S,Rhind SG,Tenn C,Bjamason S

更新日期：2016-05-01 00:00:00

abstract：:The Brazilian External Quality Assessment Program in Immunohematology (BEQAPI) was introduced with the objective of evaluating the quality of diagnosis in immunohematology. From 1992 to 2003, proficiency tests for ABO grouping, Rh (D, C, c, E, e), K phenotyping, direct antiglobulin testing (DAT), antibody screening (A...

journal_title：Journal of clinical laboratory analysis

authors： Melo L,Pellegrino J Jr,Bianco C,Castilho L

更新日期：2005-01-01 00:00:00

abstract：BACKGROUND:Technical innovation of autoimmune blistering dermatoses (ABDs) diagnosis aimed at multiplex approach. Two multiparametric ELISA tests are commercially available for ABDs serology. The aim was to compare diagnostic accuracy of multiparametric and monospecific ELISAs and to examine the diagnostic value/agreem...

journal_title：Journal of clinical laboratory analysis

authors： Gornowicz-Porowska J,Seraszek-Jaros A,Bowszyc-Dmochowska M,Bartkiewicz P,Kaczmarek E,Dmochowski M

更新日期：2018-05-01 00:00:00

abstract：:C-peptide is secreted in equimolar concentrations with insulin, and is often measured to assess pancreatic beta-cell function. C-peptide analysis is most often performed by radioimmunoassay (RIA) which has several disadvantages. We evaluated an automated, chemiluminescent immunoassay for C-peptide in terms of precisio...

journal_title：Journal of clinical laboratory analysis

authors： Hardy RW,Cohn M,Konrad RJ

更新日期：2000-01-01 00:00:00

abstract：BACKGROUND:N-terminal pro-Brain natriuretic peptide (NT-proBNP) is an important biomarker to indicate cardiac function, but its significance in cardiac surgery is still unclear. OBJECTIVE:To explore clinical significance of serum NT-proBNP and their dynamic characteristics in patients with cardiac valve replacement (C...

journal_title：Journal of clinical laboratory analysis

authors： Cai B,Wang L,Liu J,Shi Y,Guo Y

更新日期：2011-01-01 00:00:00

abstract：BACKGROUND:Early recognition and treatment for severe drug eruption are important in improving clinical outcomes. A few studies have reported laboratory parameters to evaluate the severity of drug eruptions. This study aimed to determine the association between serum ferritin and the severity of drug eruptions. METHOD...

journal_title：Journal of clinical laboratory analysis

authors： Pan Y,Tang S,Zheng S,Li S,Zhu D,Fang H,Qiao J

更新日期：2020-02-01 00:00:00

abstract：BACKGROUND:Nucleolar and spindle-associated protein 1 (NUSAP1) has been identified to be strongly implicated in the carcinogenesis of cervical carcinoma, breast cancer, and liver cancer, and shows a high expression level in bladder cancer, indicating that NUSAP1 might be a potent target for cancer treatment. Using bioi...

journal_title：Journal of clinical laboratory analysis

authors： Chen Y,Zhang W,Kadier A,Zhang H,Yao X

更新日期：2020-05-01 00:00:00

abstract：:Staphylococcus lugdunensis is an unusually virulent coagulase-negative species, associated with severe infections. The present report describes the development of a single-step, species-specific PCR protocol for S. lugdunensis identification. fbl gene, encoding a fibrinogen-binding adhesin, was exploited and assessed ...

journal_title：Journal of clinical laboratory analysis

authors： Chatzigeorgiou KS,Siafakas N,Petinaki E,Zerva L

更新日期：2010-01-01 00:00:00

abstract：BACKGROUND:Endometriosis (EMS) is a prevalent gynecological condition characterized by the growth of endometrial tissue outside the uterine cavity. This study aimed to clarify the targeted therapeutic effect of sunitinib in an endometriosis in vitro experiment. METHODS:Primary culture of ectopic endometrial cells and ...

journal_title：Journal of clinical laboratory analysis

authors： Li J,Abudula M,Fan X,Wang F,Chen Y,Liu L

更新日期：2020-11-01 00:00:00

abstract：:Using a specific serum anti-soluble T lymphocytes receptor for sheep erythrocytes (E) and SDS-PAGE, we detected radioactive bands of molecular weight 58,000 in immunoprecipitates of supernatant of heated human lymphocytes (SHL), in the supernatant of PHA stimulated lymphocyte cultures (SLC), normal human serum (NHS), ...

journal_title：Journal of clinical laboratory analysis

authors： Itano EN,Ono MA,Sumigawa M,Longo IM,Moura NC,Mendes NF

更新日期：1991-01-01 00:00:00

abstract：BACKGROUND:Iron overload is a major complication in patients with hemoglobin H (Hb H) disease and causes damage of tissues. METHODS:We investigated 26 Hb H patients and 75 controls to evaluate their oxidative stress and antioxidant statuses. RESULTS:There were significantly increased levels of superoxide anion in leu...

journal_title：Journal of clinical laboratory analysis

authors： Chiou SS,Tsao CJ,Tsai SM,Wu YR,Liao YM,Lin PC,Tsai LY

更新日期：2014-07-01 00:00:00

abstract：BACKGROUND:Salmonella is an important foodborne pathogen that causes acute diarrhea in humans worldwide. This study analyzed the relationships of serotypes and antibiotic resistance with virulence genes of Salmonella isolated from children with salmonellosis. METHODS:Serological typing was performed using the slide-ag...

journal_title：Journal of clinical laboratory analysis

authors： Yue M,Li X,Liu D,Hu X

更新日期：2020-08-14 00:00:00

abstract：:We here describe an ion-exchange high-performance liquid chromatography technique with electrochemical detection for rapid quantification of glutathione, homocysteine, cysteinylglycine, and methionine. The analytical validation of the technique showed within-assay and between-assay coefficients of variation between 3....

journal_title：Journal of clinical laboratory analysis

authors： Houze P,Gamra S,Madelaine I,Bousquet B,Gourmel B

更新日期：2001-01-01 00:00:00

abstract：BACKGROUND:Cell-free DNA (cfDNA) in the plasma of patients with both malignant and benign breast lesions was analyzed to determine whether the findings may have diagnostic and prognostic implications and to analyze the association between the levels of cfDNA and prognostic parameters. METHODS:Plasma samples were obtai...

journal_title：Journal of clinical laboratory analysis

authors： Hashad D,Sorour A,Ghazal A,Talaat I

更新日期：2012-11-01 00:00:00

abstract：BACKGROUND:Studies on gene polymorphism association are centered on childhood acute lymphoblastic leukemia (ALL), a common hematological malignancy in children younger than 16 years. Single-nucleotide polymorphisms (SNPs) in some genes, such as ARID5B and CDKN2B, are associated with the risk of childhood ALL. T-cell le...

journal_title：Journal of clinical laboratory analysis

authors： Mei E,Wei X,Gao J,Tian X,Li W,Liu L,Qian C

更新日期：2020-09-01 00:00:00

abstract：:In 23 patients with common migraine (CM), immune responsiveness and frequency of immunocompetent cells were investigated. In particular, phagocytosis and killing of Candida albicans by polymorphs (PMNs) and monocytes were analyzed. Also, the percentages of CD3+, CD4+, CD8+, natural killer, and CD15+ cells were evaluat...

journal_title：Journal of clinical laboratory analysis

authors： Covelli V,Maffione AB,Munno I,Jirillo E

更新日期：1990-01-01 00:00:00

abstract：:Vitamin D plays a vital role not only in bone health but also in pathophysiology of many other body functions. In recent years, there has been significant increase in testing of 25-hydroxyvitamin D (25-OH vitamin D), a marker of vitamin D deficiency. The most commonly used methods for the measurement of 25-OH vitamin ...

journal_title：Journal of clinical laboratory analysis

authors： Garg U,Munar A,Frazee C 3rd,Scott D

更新日期：2012-09-01 00:00:00

abstract：BACKGROUND:Low value of serum Clara cell protein (CC16) is associated with bronchial hyperreactivity in children. OBJECTIVE:To evaluate the serum CC16 in relation to atopy and previously manifested LRTD. METHODS:In the population of 163 healthy 5- to 24-month-old children, atopy was determined by Phadiatop-infant (se...

journal_title：Journal of clinical laboratory analysis

authors： Ilic N,Mihailovic N

更新日期：2017-11-01 00:00:00

abstract：BACKGROUND:Dried blood spots (DBS) are an important form of bio-sampling and valuable approach for storing blood samples for genetic studies. This has necessitated in developing an effective protocol to isolate genomic DNA (gDNA) from DBS samples.In this study, we have elucidated a dependable and non-hazardous "single ...

journal_title：Journal of clinical laboratory analysis

authors： Shaik M,Shivanna DK,Kamate M,Ab V,Tp KV

更新日期：2016-11-01 00:00:00

abstract：BACKGROUND:MYBPC3 mutations have been described in dilated cardiomyopathy (DCM) and hypertrophic cardiomyopathy (HCM). A mutation, c.3373G>A, has been reported to cause autosomal recessive form of HCM. Here, we report that this mutation can cause autosomal dominant form of DCM. METHODS:Next-generation sequencing using...

journal_title：Journal of clinical laboratory analysis

authors： Mahdieh N,Hosseini Moghaddam M,Motavaf M,Rabbani A,Soveizi M,Maleki M,Rabbani B,Alizadeh-Asl A

更新日期：2018-07-01 00:00:00

abstract：BACKGROUND:Delta neutrophil index (DNI) is a new inflammatory marker and the present study aimed to evaluate the predictive value of the DNI for the presence of a perforation in elderly with acute appendicitis. METHODS:This retrospective observational study was conducted on 108 consecutive elderly patients (≥65 years ...

journal_title：Journal of clinical laboratory analysis

authors： Shin DH,Cho YS,Kim YS,Ahn HC,Oh YT,Park SO,Won MH,Cho JH,Kim YM,Seo JY,Lee YH

更新日期：2018-01-01 00:00:00