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Exogenous interferences with Jaffe creatinine assays: addition of sodium dodecyl sulfate to reagent eliminates bilirubin and total protein interference with Jaffe methods.

Abstract：

BACKGROUND:The study evaluated the impact of interferences on the analytical specificity of three commercial and commonly used creatinine methods (two Jaffe and one enzymatic). METHODS:Manufacturer creatinine methods plus modified methods were tested with the following interferences: spiking serum with bilirubin, albumin, glucose, hemoglobin and lipid, and patient sera with maximum concentrations of bilirubin, 1,090 micromol/l and protein, 117.8 g/l. RESULTS:Hemoglobin, 7.5 g/l and lipaemic with triglyceride concentration of 6.27 mmol/l, did not interfere with all assays. Glucose >33.3 mmol/l increased creatinine recovery for Dimension method. Samples spiked with bilirubin imparted a negative bias for Dimension and Architect methods but imparted a positive bias for Vitros assay. However, using patient sera, negative bias with bilirubin was found for all methods, from which Architect method gave the highest effect (R(2)=0.861), followed by Vitros (R(2)=0.239) and Dimension (R(2)=0.163). Protein provided the positive bias for all creatinine measurements that increased with increasing concentration (R(2) ranging from 0.104 to 0.182, P<0.0001). Addition of sodium dodecyl sulfate (SDS) in alkaline-picrate reagent reduced the effect of bilirubin and protein for kinetic Jaffe method. Although adding potassium ferricyanide was well effective for eliminating negative interference of bilirubin, it was prone to interference from protein. CONCLUSIONS:Endogenous interferences continue to plague creatinine accuracy measurement in both Jaffe and enzymatic methods, and consequentially the estimated glomerular filtration rate. The addition of SDS to the alkaline-pirate reagent was shown to be effective in reducing bilirubin and protein interferences.

journal_name

J Clin Lab Anal

journal_title

Journal of clinical laboratory analysis

authors

Srisawasdi P,Chaichanajarernkul U,Teerakanjana N,Vanavanan S,Kroll MH

doi

10.1002/jcla.20350

subject

Has Abstract

pub_date

2010-01-01 00:00:00

pages

123-33

issue

eissn

0887-8013

issn

1098-2825

journal_volume

pub_type

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Exogenous interferences with Jaffe creatinine assays: addition of sodium dodecyl sulfate to reagent eliminates bilirubin and total protein interference with Jaffe methods.