Identification of plasma hsa_circ_0008673 expression as a potential biomarker and tumor regulator of breast cancer.

abstract：:A flow cytometric method for quantitation of glutathione (GSH) was applied to simultaneous analysis of the major leukocyte types in peripheral blood. Cellular thiols (predominantly GSH) were stained with monochlorobimane (MCIB), and thiol fluorescence was measured with a flow cytometer. The fluorescence of the thiols ...

journal_title：Journal of clinical laboratory analysis

authors： Scott RB,Collins JM,Matin S,White F,Swerdlow PS

更新日期：1990-01-01 00:00:00

abstract：BACKGROUND:Non-acrocentric satellited chromosomes mostly result from familial balanced insertions or translocations with p12 or p13 of any acrocentric. Although all non-acrocentrics have been involved, only 12 instances of chromosome 6 involvement are known. CASE PRESENTATION:A female infant exhibited clinical feature...

journal_title：Journal of clinical laboratory analysis

authors： Domínguez MG,Rivera H,Dávalos-Pulido RM,Dávalos-Rodríguez IP

更新日期：2020-08-01 00:00:00

abstract：:Circulating bilirubin is thought to function as a physiological antioxidant. One of the decomposition products of this process is the biopyrrins, which include two regioisomers: biotripyrrin-a (1,14,15,17-tetrahydro-2,7,13-trimethyl-1,14-deoxy-3-vinyl-16H-tripyrrin-8,12-dipropionic acid) and biotripyrrin-b (1,14,15,17...

journal_title：Journal of clinical laboratory analysis

authors： Ihara H,Matsumoto T,Morita Y,Hirano A,Okada M,Hashizume N,Shioji I,Yoshimura H

更新日期：2007-01-01 00:00:00

abstract：BACKGROUND:The human epididymis protein 4 (HE4) may have high specificity in the detection of malignant diseases, making the development of an immunoassay for HE4 essential. METHODS:In our study, a fusion gene was constructed encoded with the HE4 protein. This protein was then produced in the bacterial cells (Escheric...

journal_title：Journal of clinical laboratory analysis

authors： Zhou L,Lv Z,Shao J,Xu Y,Luo X,Zhang Y,Hu Y,Zhang W,Luo S,Fang J,Wang Y,Duan C,Huang R

更新日期：2016-09-01 00:00:00

abstract：BACKGROUND:It has been identified that incidence of infertility was about 20% among couples worldwide, about 50% caused by male elements. However, conventional semen laboratory detections could not handle clinical needs, which led to more comprehensive parameters for male fertility evaluation. We aimed to investigate t...

journal_title：Journal of clinical laboratory analysis

authors： Lu R,Chen X,Yu W,Jiang F,Zhou X,Xu Y,Wang F

更新日期：2020-09-14 00:00:00

abstract：BACKGROUND:To discover how NLRP3 and TNFRSF1A polymorphisms affect the efficacy of traditional medicine and etanercept for ankylosing spondylitis (AS) patients. METHODS:Single nucleotide polymorphism (SNP) and haplotype analyses were conducted based on determined NLRP3 and TNFRSF1A among AS patients. We subsequently a...

journal_title：Journal of clinical laboratory analysis

authors： Zhao S,Chen H,Wu G,Zhao C

更新日期：2017-11-01 00:00:00

abstract：BACKGROUND:Ursodeoxycholic acid (UDCA) has been widely recommended as the first-line drug for primary biliary cholangitis (PBC) in the current guidelines. However, its therapeutic effects are poor in nearly one-third of patients. The early identification and intervention of these patients is crucial for delaying diseas...

journal_title：Journal of clinical laboratory analysis

authors： Tian S,Liu Y,Sun K,Zhou X,Ma S,Zhang M,Zhou X,Wang L,Han Y

更新日期：2020-11-01 00:00:00

abstract：:The matrix metalloproteinase matrilysin (MMP-7) is a member of the matrix metalloproteinase gene family, which is believed to play an important role in tumor invasion and metastasis. We have previously found that matrilysin mRNA is specifically expressed in colorectal cancers and adenomas and that its message is local...

journal_title：Journal of clinical laboratory analysis

authors： Yamamoto H,Itoh F,Senota A,Adachi Y,Yoshimoto M,Endoh T,Hinoda Y,Yachi A,Imai K

更新日期：1995-01-01 00:00:00

abstract：BACKGROUND:There is a high prevalence of Hepatitis B virus (HBV) infection in China. The aim of this article is to reveal the HBV prevalence and its outcome in southern China by analyzing the HBV serum markers and their relationship to HBV genotype. METHODS:HBV serum markers, HBV-DNA and Alanine aminotransferase (ALT)...

journal_title：Journal of clinical laboratory analysis

authors： Lin S,Liu C,Shang H,Chen H,Yang B,Chen J,Chen Y,Chen D,Ou Q

更新日期：2013-03-01 00:00:00

abstract：:An automated measurement of total and free hydroxyproline in serum or urine is presented that uses flow injection analysis. After exclusion of nonspecific substances, hydroxyproline was oxidized by chloramine-T and L-cysteine with Ehrlich's reagent. The linearity obtained was from 3.8 mumole/L to 1.22 mmole/L with goo...

journal_title：Journal of clinical laboratory analysis

authors： Uji Y,Karmen A,Okabe H,Hata K,Miura M,Ozaki K,Minamizaki M,Shibata T,Inayama S

更新日期：1994-01-01 00:00:00

abstract：BACKGROUND:Reference intervals are critical for interpreting test results of a clinical laboratory. The aim of this study was to establish local reference intervals of complete blood count for healthy preschoolers in China. METHODS:Three thousand eight hundred and twenty-one blood specimens from children aged 4 months...

journal_title：Journal of clinical laboratory analysis

authors： Wang GC,Li N,Niu C,Ma WB,Wang ZL,Guo H,Han LH

更新日期：2017-09-01 00:00:00

abstract：BACKGROUND & AIM:Hepatitis B virus-e-antigen (HBeAg) is an affordable viral marker to assess viral replication kinetics and response to antiviral therapy. In the absence of confirmatory assays, discrepant or false-positive HBeAg results are resolved by screening for other HBV markers. We standardized an in-house HBeAg ...

journal_title：Journal of clinical laboratory analysis

authors： Fletcher GJ,Anantharam R,Radhakrishnan K,Karunakaran A,Abraham P

更新日期：2016-11-01 00:00:00

abstract：:Determination of free digoxin levels in patients treated with digoxin immune FAb has long been a problematic area in clinical laboratory testing. The older radioimmunoassays resulted in inaccurate and variable results due to the competition of the administered drug with the radioactively labelled forms. The 1995 Physi...

journal_title：Journal of clinical laboratory analysis

authors： Honda SA,Rios CN,Murakami L,Morita T,Scottolini AG,Bhagavan NV

更新日期：1995-01-01 00:00:00

abstract：:Quantitation of hemoglobin alpha 2 delta 2 (HbA2) is a basic and confirmatory test in diagnosing the carrier state of beta-thalassemia. The present study was designed to investigate the effect of cigarette smoking on the diagnostic reliability of HbA2. A total of 2,867 (654 smokers and 2,213 never smokers) male subjec...

journal_title：Journal of clinical laboratory analysis

authors： Tarazi IS,Sirdah MM,El Jeadi H,Al Haddad RM

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:The many techniques used to diagnose the Factor V Leiden (FVL) mutation, the most common hereditary hypercoagulation disorder in Eurasians, and the most frequently requested genetic test reflect the evolving strategies in protein and DNA diagnosis. METHODS:Here, molecular methods to diagnose the FVL mutatio...

journal_title：Journal of clinical laboratory analysis

authors： Oh H,Smith CL

更新日期：2011-01-01 00:00:00

abstract：:The objectives of this research were to establish an automatic analysis method for the determination of serum argininosuccinate lyase (ASL) and to investigate the value of serum ASL test in the diagnosis of various liver disorders. According to the chemical reaction catalyzed by ASL, an enzyme-coupled reaction system ...

journal_title：Journal of clinical laboratory analysis

authors： Feng JF,Chen TM,Wen YA,Wang J,Tu ZG

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:Circular RNAs (circRNAs) are emerging noncoding RNAs that are involved in many biological processes and diseases. The expression profile of circRNAs in preterm neonates with bronchopulmonary dysplasia (BPD) remains unresolved. METHODS:In BPD infants, peripheral venous blood was drawn and circRNAs were extra...

journal_title：Journal of clinical laboratory analysis

authors： Mao X,Guo Y,Qiu J,Zhao L,Xu J,Yin J,Lu K,Zhang M,Cheng R

更新日期：2020-07-01 00:00:00

abstract：:The immune complex transfer enzyme immunoassay for antibody IgG to HIV-1 gp41 antigen was developed using two synthetic peptides. An aliquot (10 microl) of serum samples from HIV-1 seropositive subjects was incubated simultaneously with 2,4-dinitrophenyl-bovine serum albumin-synthetic HIV-1 gp41 peptide conjugates and...

journal_title：Journal of clinical laboratory analysis

authors： Hashida S,Ishikawa S,Hashinaka K,Nishikata I,Oka S,Ishikawa E

更新日期：1998-01-01 00:00:00

abstract：:Using a specific serum anti-soluble T lymphocytes receptor for sheep erythrocytes (E) and SDS-PAGE, we detected radioactive bands of molecular weight 58,000 in immunoprecipitates of supernatant of heated human lymphocytes (SHL), in the supernatant of PHA stimulated lymphocyte cultures (SLC), normal human serum (NHS), ...

journal_title：Journal of clinical laboratory analysis

authors： Itano EN,Ono MA,Sumigawa M,Longo IM,Moura NC,Mendes NF

更新日期：1991-01-01 00:00:00

abstract：:Ninety semen samples from male patients were studied. They were classified into 4 different groups; normospermic fertile, oligospermia, asthenospermia, and oligoasthenospermia. In addition to routine semen analysis, the hypo-osmotic swelling test was done for all these samples, measuring total protein and specific gra...

journal_title：Journal of clinical laboratory analysis

authors： al-Mogazy HM,al-Khadra S,Kenawy G,al-Zeftawy A,Mostafa T,Attia A,Attia AM

更新日期：1993-01-01 00:00:00

abstract：BACKGROUND:A-kinase-interacting protein 1 (AKIP1) has been reported as an oncogenetic factor in multiple cancers; however, no study has reported its role in non-small cell lung cancer (NSCLC) yet. This study aimed to evaluate the expression of AKIP1, and its correlation with tumor characteristics as well as prognosis i...

journal_title：Journal of clinical laboratory analysis

authors： Liu Y,Tian J,Qin D,Liu J,Xie Y

更新日期：2020-04-01 00:00:00

abstract：:The proteoglycan decorin inhibits TGF-beta; therefore, it could antagonize progression of fibrotic diseases associated with activation of TGF-beta(1). The effect of decorin transfection in human mesangial cells (HMCs) on the expression of genes related to kidney fibrosis was investigated. HMCs, isolated from glomeruli...

journal_title：Journal of clinical laboratory analysis

authors： Costacurta A,Priante G,D'Angelo A,Chieco-Bianchi L,Cantaro S

更新日期：2002-01-01 00:00:00

abstract：:In 23 patients with common migraine (CM), immune responsiveness and frequency of immunocompetent cells were investigated. In particular, phagocytosis and killing of Candida albicans by polymorphs (PMNs) and monocytes were analyzed. Also, the percentages of CD3+, CD4+, CD8+, natural killer, and CD15+ cells were evaluat...

journal_title：Journal of clinical laboratory analysis

authors： Covelli V,Maffione AB,Munno I,Jirillo E

更新日期：1990-01-01 00:00:00

abstract：BACKGROUND:Low concentration C-reactive protein (CRP) has favorable prognostic significance in patients with cardiovascular risks. METHODS:We compared the wr-CRP method with the hs-CRP method both on Roche Cobas c702 analyzer for the determination of low CRP concentration (<20 mg/L) including 200 patients treated in C...

journal_title：Journal of clinical laboratory analysis

authors： Li R,Xue Y,Wang T,Gong L,Peng P,Xiong P,Dai M,Shao T,Hu Y,Ye X

更新日期：2019-10-01 00:00:00

abstract：:We have evaluated a new latex nephelometric test for the quantitation of myoglobin in human serum. The assay consists of incubating the diluted serum sample (20-fold) for 12 min at room temperature with latex particles covalently coated with anti-myoglobin antibodies and then quantifying the change of light-scatter pr...

journal_title：Journal of clinical laboratory analysis

authors： Borque L,Rus A,Ruiz R

更新日期：1991-01-01 00:00:00

abstract：:The detection of IgA and low-avidity IgG and antibodies in serum is a potentially useful marker of recent infection by a microorganism. We studied the reliability of IgG avidity and presence of IgA for the diagnosis of recent acute infection by rubella virus. Low-avidity IgG (Avy-EIA test) was determined with a modifi...

journal_title：Journal of clinical laboratory analysis

authors： Gutiérrez J,Rodríguez MJ,De Ory F,Piédrola G,Maroto MC

更新日期：1999-01-01 00:00:00

abstract：:Hemoglobin F (HbF) is an effective inhibitor of HbS polymerization. Hydroxyurea (HU) is used to increase HbF synthesis and improve the clinical course of sickle cell disease (SCD) patients. We studied a series of laboratory parameters concerning HbF production and reticulocyte response, and compared data between two g...

journal_title：Journal of clinical laboratory analysis

authors： Borba R,Lima CS,Grotto HZ

更新日期：2003-01-01 00:00:00

abstract：BACKGROUND:MicroRNAs play a vital role in coronary artery disease. Abnormal expression of microRNAs has been found to be associated with the occurrence of CAD. METHODS:We identified significantly differentially expressed microRNAs in plasma between 40 patients with CAD and 10 controls with NCA using RNA sequencing. Th...

journal_title：Journal of clinical laboratory analysis

authors： Zhong Z,Zhong W,Zhang Q,Zhang Q,Yu Z,Wu H

更新日期：2020-01-01 00:00:00

abstract：BACKGROUND:Previous studies suggested that adiponectin (APN) could ameliorate ischemia/reperfusion injury and endothelial dysfunction in patients with acute myocardial infarction. However, the relationship between serum APN level and coronary flow after primary percutaneous coronary intervention (PPCI) in patients with...

journal_title：Journal of clinical laboratory analysis

authors： Liang S,Li H,Shen X,Liu R

更新日期：2019-06-01 00:00:00

abstract：:The direct agglutination test (DAT) and the enzyme-linked immunosorbent assay (ELISA) were used for serodiagnosis of parasitologically confirmed Indian visceral leishmaniasis (VL) cases. All the sera of VL cases were positive by both the methods. DAT titres of VL cases were greater than or equal to 1:3,200, and ELISA ...

journal_title：Journal of clinical laboratory analysis

authors： Pal A,Mukerji K,Basu D,Naskar K,Mallick KK,Ghosh DK

更新日期：1991-01-01 00:00:00