Cost-Effective In-House Neutralization Assay for the Confirmation of HBeAg.

Abstract:

BACKGROUND & AIM:Hepatitis B virus-e-antigen (HBeAg) is an affordable viral marker to assess viral replication kinetics and response to antiviral therapy. In the absence of confirmatory assays, discrepant or false-positive HBeAg results are resolved by screening for other HBV markers. We standardized an in-house HBeAg neutralization assay (HBeAg-NT) to confirm HBeAg in clinical samples. METHODS:The performance and reliability of this assay were evaluated by first WHO International Standard for HBeAg (first WHO-IS HBeAg) from Paul Ehrlich Institute and clinical samples (n = 150) from chronic HBV carriers. Of these, 71 HBeAg-positive sera were used for HBeAg-NT. RESULTS:Concentrations spanning 0.25-10 U of first WHO-IS HBeAg and clinical samples (S/Co ranges from 1.00 to 10.00) were neutralized completely in the HBeAg-NT. CONCLUSIONS:HBeAg-NT is a simple, cost-effective, and reliable direct approach to confirm HBeAg in clinical samples which precludes the need for screening additional HBV markers in low resource settings.

journal_name

J Clin Lab Anal

authors

Fletcher GJ,Anantharam R,Radhakrishnan K,Karunakaran A,Abraham P

doi

10.1002/jcla.21995

subject

Has Abstract

pub_date

2016-11-01 00:00:00

pages

1146-1149

issue

6

eissn

0887-8013

issn

1098-2825

journal_volume

30

pub_type

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