Detection of Human Epididymis Protein 4 (HE4) in Human Serum Samples Using a Specific Monoclonal Antibody-Based Sandwich Enzyme-Linked Immunosorbent Assay (ELISA).

abstract：BACKGROUND:Oxidative stress is an imbalance between the reactive oxygen species and antioxidant system. In this study, total oxidative stress (TOS) and total antioxidant capacity (TAC) were investigated with a new and practical method in childhood iron-deficiency anemia. METHOD:During the study period 80 children betw...

journal_title：Journal of clinical laboratory analysis

authors： Akça H,Polat A,Koca C

更新日期：2013-05-01 00:00:00

abstract：OBJECTIVE:To explore the role of serum periostin in patients with aneurysmal subarachnoid hemorrhage (aSAH). METHOD:We conducted a retrospective study and 124 aSAH patients treated in Shenzhen People's hospital during March 1st 2015 to December 30th 2016 were included. Baseline information, neurological status and cli...

journal_title：Journal of clinical laboratory analysis

authors： Luo W,Wang H,Hu J

更新日期：2018-06-01 00:00:00

abstract：AIM:The present study aimed to investigate associations of the platelet-to-white blood cell ratio (PWR)-a novel hematological indicator of inflammatory responses-with 30-day outcomes in patients with HBV-associated decompensated cirrhosis (HBV-DeCi). METHODS:We recruited 131 patients with HBV-DeCi for this retrospecti...

journal_title：Journal of clinical laboratory analysis

authors： Zhang J,Qiu Y,He X,Mao W,Han Z

更新日期：2020-09-07 00:00:00

abstract：BACKGROUND:The performances of the QMS(®) Teicoplanin immunoassay recently developed on Cobas(®) 6000/8000 systems were evaluated and compared to a fluorescence polarization immunoassay (FPIA) [Teicoplanin Innofluor(®) Assay (Thermo Fisher Scientific, Indianapolis, IN)] on FLX analyzer (Abbott Laboratories, Abbott Park...

journal_title：Journal of clinical laboratory analysis

authors： Dailly E,Fraissinet F,Deslandes G,Bouquié R,Jolliet P

更新日期：2013-03-01 00:00:00

abstract：BACKGROUND:Automated flow cytometry-based urine analyzer is increasingly being used to identify and enumerate cells and particles in urine specimens. It measures electrical conductivity which could be transformed to osmolality. Using this machine, all urine specimens could be screened for osmolality without requiring a...

journal_title：Journal of clinical laboratory analysis

authors： Yoo DW,Lee SM,Moon SY,Kim IS,Chang CL

更新日期：2020-09-24 00:00:00

abstract：:The immune complex transfer enzyme immunoassay for antibody IgG to HIV-1 gp41 antigen was developed using two synthetic peptides. An aliquot (10 microl) of serum samples from HIV-1 seropositive subjects was incubated simultaneously with 2,4-dinitrophenyl-bovine serum albumin-synthetic HIV-1 gp41 peptide conjugates and...

journal_title：Journal of clinical laboratory analysis

authors： Hashida S,Ishikawa S,Hashinaka K,Nishikata I,Oka S,Ishikawa E

更新日期：1998-01-01 00:00:00

abstract：BACKGROUND:Toxocariasis is the clinical term that is applied to infection in the human host with Toxocara species larvae. Serological tests are important tools for the diagnosis of toxocariasis. The aim of this study was to evaluate the excretory-secretory (ES) antigens of T. cati larvae using enzyme-linked immunosorbe...

journal_title：Journal of clinical laboratory analysis

authors： Zibaei M,Sadjjadi SM,Sarkari B,Uga S

更新日期：2016-05-01 00:00:00

abstract：:The human insulin domains, signal peptide, B-chain, C-peptide, and A-chain, were highly expressed in Escherichia coli as recombinant proteins N-terminally fused to glutathione-S-transferase and a histidine-hexapeptide. The recombinant proteins were purified from insoluble cell fraction by affinity chromatography using...

journal_title：Journal of clinical laboratory analysis

authors： Walter M,Berg H,Northemann W

更新日期：1994-01-01 00:00:00

abstract：:One of the most significant achievements of the biochemist during the past two decades is the use to which immunologically based assays have been put in clinical diagnosis (Hood et al.: Immunology, 1984). The problem faced and surmounted by immunologists in effecting the transition from research tool to routine clinic...

journal_title：Journal of clinical laboratory analysis

authors： Nicholls PJ,Malcolm AD

更新日期：1989-01-01 00:00:00

abstract：:As several possible prognostic and therapeutic applications of interleukin-6 are currently under trial, the available methods for its quantification in biological fluids should be evaluated. In this report, the 7TD1 hybridoma bioassay is compared to an enzyme immunoassay for the determination of interleukin-6 in serum...

journal_title：Journal of clinical laboratory analysis

authors： Papadopoulos NG,Dedoussis GV,Baxevanis CN,Papamichail M

更新日期：1995-01-01 00:00:00

abstract：:The use of serum alkaline phosphatase (ALP) isoenzymes as markers of breast cancer metastases and treatment efficacy has received little attention. Twenty-six breast cancer women (56+/-13 years, all post-menopausal) were prospectively evaluated during their first and third course of chemotherapy (4-week interval). Ser...

journal_title：Journal of clinical laboratory analysis

authors： Le Bricon T,Gay-Bellile C,Cottu P,Benlakehal M,Guillon H,Houzé P

更新日期：2010-01-01 00:00:00

abstract：BACKGROUND:The aim of this study was to explain the effects of microRNA-132 in renal cell carcinoma by regulating FOXM1 expression. METHODS:Thirty patients with renal cell carcinoma admitted to our hospital were enrolled, and their adjacent normal tissues and cancer tissues were taken. The expression of microRNA-132 w...

journal_title：Journal of clinical laboratory analysis

authors： Yu Y,Lu W,Zhou X,Huang H,Shen S,Guo L

更新日期：2020-01-01 00:00:00

abstract：:The technique that allows for in situ detection of PCR-amplified DNA and cDNA has improved remarkably quickly over the past 5 years. As of this writing, one can expect good results after a relatively quick "learning curve." The technique demands some skill in working with glass slides and cells/tissues. Thus, I recomm...

journal_title：Journal of clinical laboratory analysis

authors： Nuovo GJ

更新日期：1996-01-01 00:00:00

abstract：BACKGROUND:Little is known about the involvement of herpes simplex virus (HSV) or Mycobacterium tuberculosis (MTB) as potentially curable causes of central nervous system (CNS) infections in sub-Saharan Africa. OBJECTIVE:In this study, we developed a PCR assay dedicated to simultaneous testing of HSV1/HSV2 and MTB in ...

journal_title：Journal of clinical laboratory analysis

authors： Zida S,Kolia-Diafouka P,Kania D,Sotto A,Foulongne V,Bolloré K,Ouangraoua S,Méda N,Carrère-Kremer S,Van de Perre P,Tuaillon E

更新日期：2019-03-01 00:00:00

abstract：BACKGROUND:Delta neutrophil index (DNI) is a new inflammatory marker and the present study aimed to evaluate the predictive value of the DNI for the presence of a perforation in elderly with acute appendicitis. METHODS:This retrospective observational study was conducted on 108 consecutive elderly patients (≥65 years ...

journal_title：Journal of clinical laboratory analysis

authors： Shin DH,Cho YS,Kim YS,Ahn HC,Oh YT,Park SO,Won MH,Cho JH,Kim YM,Seo JY,Lee YH

更新日期：2018-01-01 00:00:00

abstract：BACKGROUND:This study was performed to investigate the epidemiology of enterovirus (EV) infections in children at a single hospital during recent 5 years. This study was preformed from December 2008 to November 2013. METHODS:We subjected 1,067 cerebrospinal fluid (CSF) specimens from 1,053 patients and 678 stool speci...

journal_title：Journal of clinical laboratory analysis

authors： Jeon JS,Kim JK,Yu J,Kim JW

更新日期：2016-03-01 00:00:00

abstract：:Enteroviruses (EVs) are the most commonly identified cause of aseptic meningitis. Rapid detection and characterization of EV meningitis is essential in making decisions for patient management and treatment. A total of 52 cases of acute aseptic meningitis that occurred from March 2003 to April 2005 were investigated fo...

journal_title：Journal of clinical laboratory analysis

authors： Dumaidi K,Frantzidou F,Papa A,Diza E,Antoniadis A

更新日期：2006-01-01 00:00:00

abstract：:Adenosine deaminase (ADA) exists as two isoenzymes, ADA(1) and ADA(2). It appears that the ADA(2) isoenzyme originates mainly from monocytes and macrophages. In tuberculous pleural effusions most of the ADA activity consists of ADA(2). The aim of this prospective study was to analyse ADA isoenzymes in the CSF of patie...

journal_title：Journal of clinical laboratory analysis

authors： Schutte CM,Ungerer JP,du Plessis H,van der Meyden CH

更新日期：2001-01-01 00:00:00

abstract：:A microparticle-enhanced nephelometric immunoassay was developed for plasminogen quantitation in human plasma. It is based on the nephelometric measurement of the light scattered by microparticle clusters formed during a sandwich reaction between plasminogen, microparticle--anti-plasminogen conjugate, and the free ant...

journal_title：Journal of clinical laboratory analysis

authors： Marchal E,Montagne P,Cuillière ML,Béné MC,Faure G

更新日期：1996-01-01 00:00:00

abstract：BACKGROUND:Osteoarthritis (OA) is a popular arthrosis featured as pain, limited joint activity, and deformity. Cyclooxygenase-2 (COX-2) has been reported to be up-regulated in arthritic tissues and is integral to the progression of osteoarthritis (OA). Previous studies showed the COX-2 promoter G-765C polymorphism coul...

journal_title：Journal of clinical laboratory analysis

authors： Huang W,Deng C,Tian F,Gao W,Ding Z,Rao X,Yin Z

更新日期：2018-07-01 00:00:00

abstract：BACKGROUND:Acute respiratory distress syndrome (ARDS) is a critical condition characterized by bilateral pulmonary infiltrates and severe hypoxemia. This study aimed to evaluate the diagnostic and prognostic values of Club cell protein 16 (CC16) in critical care patients with ARDS. METHODS:In this retrospective observ...

journal_title：Journal of clinical laboratory analysis

authors： Lin J,Zhang W,Wang L,Tian F

更新日期：2018-02-01 00:00:00

abstract：BACKGROUND:The sialylation of serum proteins and lipids changes in liver diseases of different etiologies and could change the total sialic acid (TSA), lipid-bound SA (LSA), and free SA (FSA) levels in the sera. However, little is known of the relationship of serum SAs concentrations and the severity of liver disease. ...

journal_title：Journal of clinical laboratory analysis

authors： Chrostek L,Supronowicz L,Panasiuk A,Cylwik B,Gruszewska E,Szmitkowski M

更新日期：2014-11-01 00:00:00

abstract：:It is believed that boundary compositions of matrix proteins might play a role in stone formation; however, few proteomic studies concerning matrix proteins in urinary stones have been conducted. In this study, we extracted low molecular weight proteins from calcium oxalate stones and measured their characteristic pat...

journal_title：Journal of clinical laboratory analysis

authors： Chen WC,Lai CC,Tsai Y,Lin WY,Tsai FJ

更新日期：2008-01-01 00:00:00

abstract：BACKGROUNDS:Shortening of primary cilia in kidney epithelial cells is associated with kidney injury and involved with the induced level of α-tubulin in urine. Therefore, rapid detection and quantification of α-tubulin in the urine samples could be used to the preliminary diagnosis of kidney injury. METHODS:Cellulose-b...

journal_title：Journal of clinical laboratory analysis

authors： Choi ES,Al Faruque H,Kim JH,Cho JH,Park KM,Kim E

更新日期：2020-01-01 00:00:00

abstract：:In this study, the effect of the synthetic glucocorticoid hormone dexamethasone (DXM) on HSV replication was studied in a DXM receptor-positive mouse neuroblastoma (NB) cell line. In cells treated with 10(-7) M DXM and then infected with HSV, there was a statistically significant 9-18-fold increase in the amount of vi...

journal_title：Journal of clinical laboratory analysis

authors： Sawiris GP,Sydiskis RJ,Bashirelahi N

更新日期：1994-01-01 00:00:00

abstract：:Radioimmunoassay (RIA) that involves purification of the analyte by organic solvent extraction is widely used. Although the extraction RIA is reliable when properly validated, it is time consuming and radioactive, we measure urinary total testosterone with a highly sensitive rapid and accurate time-resolved fluorescen...

journal_title：Journal of clinical laboratory analysis

authors： Bao S,Peng Y,Sheng S,Lin Q

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:Reference intervals are important for interpretation of clinical laboratory tests. The platelet (PLT) indices such as the mean platelet volume (MPV) and platelet distribution width (PDW) are newer hematological parameters, which have been recently reported as clinically valuable biomarkers. However, there ar...

journal_title：Journal of clinical laboratory analysis

authors： Hong J,Min Z,Bai-shen P,Jie Z,Ming-ting P,Xian-zhang H,Xiao-ke H,Lan-lan W,Xin Z,Wei G,Rui Q,Wen-xiang C,Xin-zhong W,Yue-yun M,Hong S

更新日期：2015-01-01 00:00:00

abstract：:The levels of several soluble cytokine receptors in body fluids of healthy individuals change with age. Clinical application of the measurement of the serum soluble interleukin-1 receptor type I (sIL-1RI) level depends critically on the samples used as the controls. At present, there is no information regarding the le...

journal_title：Journal of clinical laboratory analysis

authors： Okamoto Y,Tanaka M,Miyahara N,Tanabe M,Gotoh Y,Nagai T,Fujita N,Fukui T,Masuzawa T

更新日期：2009-01-01 00:00:00

abstract：:We studied a new proteinase K assay method for human serum mitochondrial aspartate aminotransferase. We found that proteinase K showed no inactivation of human mitochondrial aspartate aminotransferase isoenzyme and complete inactivation of cytosolic aspartate aminotransferase. Previous studies have shown that selectiv...

journal_title：Journal of clinical laboratory analysis

authors： Watazu Y,Uji Y,Okabe H,Shirahase Y,Kaneda N,Karmen A

更新日期：1993-01-01 00:00:00

abstract：BACKGROUND:The GABA-A receptor signaling pathway regulates proliferation, differentiation, apoptosis, and responses to overt DNA damage during embryonic development. METHODS:To analyze the gene expression after intracytoplasmic sperm injection (ICSI) and in in vivo mouse embryos, the global pattern of gene expression ...

journal_title：Journal of clinical laboratory analysis

authors： Zhang Z,Wang T,Huang J,Huang Y,Zhang Q

更新日期：2020-09-20 00:00:00