Abstract:
:A microparticle-enhanced nephelometric immunoassay was developed for plasminogen quantitation in human plasma. It is based on the nephelometric measurement of the light scattered by microparticle clusters formed during a sandwich reaction between plasminogen, microparticle--anti-plasminogen conjugate, and the free antibodies of anti-plasminogen rabbit antiserum. This immunoassay was sensitive (detection limit in reaction mixture, 34 micrograms/L) and could be performed in 500-fold diluted human plasma, excluding any interference or sample pretreatment. It allowed the quantitation of plasminogen on a large range of concentrations (17-550 mg/L), with a security in antigen excess reaching 1,100 mg/L, with accuracy (linear recovery in dilution-overloading assay and correlation with conventional immunonephelometry), and precision (within- and between-run coefficients of variation lower than 8%). A normal reference range from 54 to 148 mg/L (mean +/- 2 SD) was calculated from plasminogen concentration in plasma from 130 adults. Easy to perform (no washing or phase separation) and rapid (two steps of 30 minutes then 1 hour of incubation at room temperature), this microparticle-enhanced nephelometric immunoassay could be an interesting alternative method for human plasminogen quantitation.
journal_name
J Clin Lab Analjournal_title
Journal of clinical laboratory analysisauthors
Marchal E,Montagne P,Cuillière ML,Béné MC,Faure Gdoi
10.1002/(SICI)1098-2825(1996)10:2<85::AID-JCLA5>3.subject
Has Abstractpub_date
1996-01-01 00:00:00pages
85-90issue
2eissn
0887-8013issn
1098-2825pii
10.1002/(SICI)1098-2825(1996)10:2<85::AID-JCLA5>3.journal_volume
10pub_type
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